1.An Insight of the First Community Infected COVID-19 Patient in Beijing by Imported Case: Role of Deep Learning-Assisted CT Diagnosis.
Da Sheng LI ; Da Wei WANG ; Na Na WANG ; Hai Wang XU ; He HUANG ; Jian Ping DONG ; Chen XIA
Chinese Medical Sciences Journal 2021;36(1):66-71
In the era of coronavirus disease 2019 (COVID-19) pandemic, imported COVID-19 cases pose great challenges to many countries. Chest CT examination is considered to be complementary to nucleic acid test for COVID-19 detection and diagnosis. We report the first community infected COVID-19 patient by an imported case in Beijing, which manifested as nodular lesions on chest CT imaging at the early stage. Deep Learning (DL)-based diagnostic systems quantitatively monitored the progress of pulmonary lesions in 6 days and timely made alert for suspected pneumonia, so that prompt medical isolation was taken. The patient was confirmed as COVID-19 case after nucleic acid test, for which the community transmission was prevented timely. The roles of DL-assisted diagnosis in helping radiologists screening suspected COVID cases were discussed.
Adult
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Beijing
;
COVID-19/diagnostic imaging*
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COVID-19 Testing/methods*
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Community-Acquired Infections/diagnostic imaging*
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Deep Learning
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Humans
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Lung/diagnostic imaging*
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Male
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Tomography, X-Ray Computed/methods*
3.Establishment and preliminary application of quantitative real-time PCR assay for the detection of SARS-CoV-2 subgenomic nucleocapsid RNA.
Xiao Juan ZHU ; Yin CHEN ; Bin WU ; Yi Yue GE ; Tao WU ; Qiao QIAO ; Kang Chen ZHAO ; Lun Biao CUI
Chinese Journal of Preventive Medicine 2023;57(2):268-272
Objective: To establish a rapid and specific quantitative real-time PCR (qPCR) method for the detection of SARS-CoV-2 subgenomic nucleocapsid RNA (SgN) in patients with COVID-19 or environmental samples. Methods: The qPCR assay was established by designing specific primers and TaqMan probe based on the SARS-CoV-2 genomic sequence in Global Initiative of Sharing All Influenza Data (GISAID) database. The reaction conditions were optimized by using different annealing temperature, different primers and probe concentrations and the standard curve was established. Further, the specificity, sensitivity and repeatability were also assessed. The established SgN and genomic RNA (gRNA) qPCR assays were both applied to detect 21 environmental samples and 351 clinical samples containing 48 recovered patients. In the specimens with both positive gRNA and positive SgN, 25 specimens were inoculated on cells. Results: The primers and probes of SgN had good specificity for SARS-CoV-2. The minimum detection limit of the preliminarily established qPCR detection method for SgN was 1.5×102 copies/ml, with a coefficient of variation less than 1%. The positive rate of gRNA in 372 samples was 97.04% (361/372). The positive rates of SgN in positive environmental samples and positive clinical samples were 36.84% (7/19) and 49.42% (169/342), respectively. The positive rate and copy number of SgN in Wild strain were lower than those of SARS-CoV-2 Delta strain. Among the 25 SgN positive samples, 12 samples within 5 days of sampling time were all isolated with virus; 13 samples sampled for more than 12 days had no cytopathic effect. Conclusion: A qPCR method for the detection of SARS-CoV-2 SgN has been successfully established. The sensitivity, specificity and repeatability of this method are good.
Humans
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SARS-CoV-2/genetics*
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COVID-19/diagnosis*
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Subgenomic RNA
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Real-Time Polymerase Chain Reaction/methods*
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RNA, Viral/genetics*
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Sensitivity and Specificity
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Nucleocapsid/chemistry*
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COVID-19 Testing
4.Fecal Nucleic Acid Test as a Complementary Standard for Cured COVID-19 Patients.
Mei HAN ; Jing Bo ZOU ; Huan LI ; Xiao Yu WEI ; Song YANG ; Hui Zheng ZHANG ; Peng Sen WANG ; Qian QIU ; Le Le WANG ; Yao Kai CHEN ; Pin Liang PAN
Biomedical and Environmental Sciences 2020;33(12):935-939
Adolescent
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Adult
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Aged
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COVID-19/virology*
;
COVID-19 Nucleic Acid Testing/methods*
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Child
;
Coronavirus Nucleocapsid Proteins/genetics*
;
Feces/virology*
;
Female
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Humans
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Male
;
Middle Aged
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Phosphoproteins/genetics*
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RNA, Viral/genetics*
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SARS-CoV-2/isolation & purification*
;
Young Adult
5.Environmental Monitoring of A Laboratory for New Coronavirus Nucleic Acid Testing.
Pei Yong NING ; Ai Ping YU ; Yuan WANG ; Li Ru GUO ; Dan SHAN ; Mei KONG ; Ling Qi YU ; Li Kun LYU ; Ming ZOU ; Xu SU ; Chun Nan FEI ; Yu Hui ZHOU ; Bai Qi WANG
Biomedical and Environmental Sciences 2020;33(10):771-774