Objective To investigate the influence of thymosin injection combined with chemotherapy on the immune function and quality of life in patients with ovarian cancer. Methods A total of 206 patients with ovarian cancer were chosen and were divided into the control group (n = 103) and the observation group (n = 103) according to the random number table. The control group received chemotherapy. Based on the treatment of control group, the observation group was additionally treated with thymosin injection, the immune function and quality of life were analyzed and compared between two groups. Results The rates of effectivity and disease control were higher in the observation group than those in the control group [44.66 % (46/103) vs 30.10 % (31/103), 86.41 % (89/103) vs 66.99 % (69/103)], the differences were statistically significant (χ2 =3.942, 4.138, P = 0.022, 0.015), and number of cases of adverse reactions were lower in observation group than those in the control group (22 cases vs 35 cases, χ2= 3.601, P = 0.026), and the improvement rate of quality of life of patients were higher in the observation group than that in the control group [60.19 % (62/103) vs 40.78 % (42/103), χ2 = 3.974, P = 0.021]. After treatment, the expression levels of IgA, IgG, IgM and CD3, CD4, CD4/CD8, NK cells were significantly higher in the observation group than those in the control group, the difference was statistically significant (all P < 0.05). Conclusion Thymosin injection combined with chemotherapy is helpful to improve the immune function and the quality of life in patients with ovarian cancer, it is worthy of wider promotion and application.

Objective To discuss action of microelement Se in serum and milk of gestational diabetes melli-tus parturient.Methods The serum and milk samples were collected from 50 gestational diabetes mellitus parturient and 50 healthy parturient.The selenium contents were detected by radioimmunoassay.Results The serum Se content in gestational diabetes mellitus parturient was obviously lower than that in healthy parturient(t =2.128,P <0.05). The milk Se content in gestational diabetes mellitus parturient was lower than that in healthy parturient(t =1.014, P >0.05).Conclusion Selenium deficiency persisted in gestational diabetes mellitus parturient.But Selenium defi-ciency did not exist in milk of gestational diabetes mellitus parturient.So encouraging breastfeeding,elevating selenium containing food intake,and supplement the selenium preparation properly maybe prevent and delay type 2 diabetes in gestational diabetes mellitus parturient.

Objective To investigate the influence of Norcantharidin(NCTD)on apoptosis-related gene expression of gastric cancer cell line SGC-7901.Methods The experiment was divided into control group,5-Fu group,NCTD group and 5-Fu+NCTD group.The inhibitory rate,apoptosis rate and expression of survivin,bcl-2,and caspase-3 were detected by MTT assay,flow cytometry and SABC immunohistochemical method,respectively.Results NCTD showed that the inhibitory effect on growth of SGC-7901 cells with a dose-and time-effective dependent manner.The apoptotic rate increased from(8.30?1.49)% to(20.56? 1.32)%.The expressions of survivin decreased from(86.57?4.39)% to(26.11?2.27)% and bcl-2 from(85.35? 3.25)% to(30.26?1.83)%,while caspase-3 increased from(54.49?3.07)% to(92.78?2.47)%,5-Fu had the synergistic effects with NCTD.Conclusion NCTD can inhibit the growth of SGC-7901 cell lines.5-Fu had the synergistic effects with NCTD.The mechanism might correlate with induction of cell apoptosis via effect of the expression of apoptotic-related genes such as survivin,bcl-2 and caspase-3.

Mitogen-activated protein kinase (MAPK) s a serine/tyrosine kinase which s expressed widely and regulates various signal transduction pathways in mammalian cell. As one of the key branches of MAPK pathway, p38 MAPK signaling pathway plays an important role in various physiological and pathological processes, such as cell proliferation, differentiation, apoptosis and cell cycle regulation. In recent years, the research on p38MAPK signaling pathway in cell growth, metabolism and function of osteoclast, osteoblast and chondrocyte related to bone metabolism has attracted increasing attention. This article reviews the relationship beween p38MAPK and bone metabolism, in order to investigate the mechanism of action of p38MAPK in bone metabolism related diseases.

Objective To investigate the situation and demands of the nursing services for both urban and rural elderly people with chronic diseases in China,so as to provide basis of establishing the new elderly care mode. Methods 1902 urban and rural elderly with chronic diseases in almost 29 provinces had been enrolled by the convenience and stratified sampling method to complete the investigation. Results The utilization of relevant nursing care and the demand of psychological counseling and hospice care in cities and towns were higher than those of villages.The demand of common chronic diseases in towns were all higher than that in cities and villages.The demand of pain management in cities wee lower than that in towns and villages.73.3％ of elderly in the survey will choose community to subsequent recovery after discharge.The physical,psychological health and economic status might be the pivotal factors. Conclusions At present,endowment in community becomes an inevitable trend in China.Meanwhile,there are still lots of problems in our community health care services,so it remind relevant staffs that some measures should be taken to change such situations.

[Summary] With the development of diagnosis and treatment levels and the improvement of survival rates of breast cancer , related lymphedema has received increasing attention .In a long term, it is regarded as the primary complication after the breast cancer therapy , which affects the quality of life of patients .Due to lack of consensus in many aspects worldwide , it continues to be a challenge to diagnose and treat the disease .This article aimed to summarize the diagnosis and therapeutics of breast cancer related lymphedema .

Objective To give a summary of the current researches on hematopoietic stem cell niche.Methods Through extensive reviewing the related domestic and abroad literatures,the present summary reviews the compo- nents of hematopoietic stem cell niche,related cell factors and related cell signaling pathway participating in regula- tion of hematopoietic stem cell.Results The activities of hematopoietic stem cells are regulated by the niche(micro- environment),which is composed of hematopoietic stem cell and its surrounding cells.The regulation cannot be completed through one signaling pathway.Also,the self-renewal and differentiation of hematopoietic stem cell can- not be completed only through osteoblastic cells.Conclusion The niche regulates hematopoietic stem cells by differ- ent ways.With study in-depth,we will comprehensively understand the nature of stem cells and the study will pro- vide a broader space to stem cell-based therapy.

AIM: To establish a method of simultaneously determining ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 in Tangniaole Granule (Radix Trichosanthis, Rhizoma Dioscoreae, Radix et Rhizoma Ginseng Rubra, Rhizoma Anemarrhenae, Radix Astragali, etc.). METHODS: Three components: ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Tangniaole Granule were determined simultaneously by HPLC, using a Diamonsil C_ 18 ( 4.6 mm?250 mm, 5 ?m), acetonitrile-water as a mobile phase. The detection wavelength was at 203 nm. RESULTS: The relationship among the concentrations and the peak areas of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were linear respectively. The RSD of precision, stability, repeatability and recovery were all less than 3%. CONCLUSION: The method is simultaneous determination for three components in Tangniaole Granule, and can be applied to the quality control of Tangniaole Granule.

China is entering into an aging society, and the incidence of hypertension and atherosclerosis were increased year by year. The incidence of transient ischemic attack (TIA) patients with carotid stenosis increased year by year. Carotid artery stenosis or occlusion can cause reduction of local brain tissue perfusion resulting from TIA. CT cerebral perfusion imaging and SPECT could detect earlier the chronic hyper fusion of carotid stenosis TIA, forecast risk coefficient and intervention and prevent the occurrence of cerebral infarction, to evaluate effect of carotid endarterectomy and carotid s carotid artery stenting. It is very important to monitor cerebral hemodynamic changes. The technology has the features of blood infusion situation, advantages, curative effect evaluation and development trend in carotid stenosis TIA reviewed by CT perfusion imaging and SPECT.

0bjective: To observe the effects of parotid saliva(PARS), extraparotid saliva(EPS) and purified high molecular weight mucin(MG1) on the aggregation of streptococcus mutans . Methods: PARS, EPS and MG1 were prepared routinely and applied in the cultures of S. mutans Inbritt, S.mutans LM and S.sobrinus OMZ 176 respectively for 2 h. The aggregation of the bacteria was measured spectrophotometrically.Results: The aggregation (%) of S.mutans Inbritt, S.mutans LM and S.sobronus OMZ 176 induced by EPS was 32.80 , 57.87 and 35.46 , that by MGI 25.68, 32.77 and 24.16 , that by PARS 13.52, 22.23 and 16.00, respectively. Conclusion: The effect of MGI on the aggregation of streptoccus mutans is weaker than that of EPS and stronger than that of PARS. The aggregation may be primarily induced by mucins.