2.Bioactivity of several podophyllotoxin analogues
Yanqing LIU ; Shougang ZHANG ; Jie CHENG ; Hang XIAO ; Rong GAO
Journal of Medical Postgraduates 2003;0(03):-
Objective: The bioactivity of four podophyllotoxin analogues were tested against 3rd instar larvae of Culex pipiens pallens and 5 h instar larvae of Pieris rapae L.Methods:WHO bioassay and leaf-dlpping method. Results: ①Deoxypodophyllotoxin and ?-apopicropodophyllotoxin exhibited toxicity(against) Culex pipiens pallens,and their LC_(50) were 0.001 48 and 0.001 68 g/L,respectively.②All the four podophyllotoxin analogues displayed inhibitory effect on the growth and development against Culex pipiens pallens,their pupation rates were delayed comparing with control.③Deoxypodophyllotoxin,?-apopicropodophyllotoxin and Podophyllotoxin exhibited toxicity against Pieris rapae L,the LC_(50) 96 h after treatment were 0.045 4?0.078 2 and 0.159 7 g/L,respectively.④All the four podophyllotoxin analogues showed antifeedant activity against Pieris rapae L,their AFC_(50)were 0.016 1,0.018 7,0.039 4 and(0.273 9) g /L,respectively.⑤All four podophyllotoxin analogues displayed inhibitory effect on the growth and development against Pieris rapae L,but the extent of each compound were very different. Conclusion: Based on the data obtained in this investigation,it is possible that the dissimilarity in the structure of the analogues leads to their different bioactivity.
3.Effect of short hairpin RNA on growth and proliferation of human bone marrow mesenchymal stem cells
Jie CHENG ; Zezhang TAO ; Honggang DUAN ; Bokui XIAO ; Shiming CHEN
Chinese Journal of Tissue Engineering Research 2008;12(16):3158-3162
BACKGROUND:Tetramethylpyrazine (TMP) can inhibit the expression of vascular endothelial growth factor (VEGF), but it is uncertain that TMP inhibit the growth and proliferation of HL-60 leukemic cells induced by VEGF.OBJECTIVE:To observe the effect of TMP on the proliferation of HL-60 leukemic cells induced by VEGF.DESIGN:Repetitive measurement and observation.SETTING:School of Medicine, Wuhan University of Science and Technology.MATERIALS:The experiment was carried out in the Molecular Biology Laboratory Center, School of Medicine, Wuhan University of Science and Technology from March to June in 2007. Human leukemic cell line HL-60 cells were purchased from Shanghai Institute of Cell Biology. TMP hydrochloride injection was produced by Wuxi Seventh Pharmaceutical Products Limited (Lot number:011014), protamine sulfate injection was produced by Shanghai First Biochemical Pharmaceuticals (Batch number:010302), and immunohistochemistry kit was purchased from Boster company.METHODS:①Human leukemic cell line HL-60 cells at log phase were used for the experiments. Cells were treated with 100 μg/L VEGF, and then TMP at final concentrations of 1.5, 15, 150 mg/L was added into culture medium. While the cells in medium without TMP were taken as blank control group, and the cells in medium with 20 mg/L protamine as positive control group. Meanwhile cells without treatment of VEGF were served as VEGF control group. After cells were incubated for 48 hours, the growth inhibiting rate of HL-60 cells was detected by MTT assay.②After HL-60 cells were treated with TMP at the final concentrations of 1.5, 15, 150 mg/L for 24 hours, the protein expression of VEGF in HL-60 cells was examined by SP immunohistochemistry.MAIN OUTCOME MEASURES:①Growth inhibiting rate of HL-60 cells.②Protein expression of VEGF.RESULTS:①Growth inhibiting rate of HL-60 cells:After HL-60 cells induced by VEGF were treated with 15 and 150 mg/L TMP, the absorbance value was significantly lower than that in VEGF control group (P < 0.05).②Protein expression of VEGF:After HL-60 cells were treated with TMP for 24 hours, the protein expression of VEGF was down-regulated with increasing TMP concentration in a dependent manner. Significant differences were observed in the protein expression of VEGF between cells treated by TMP and the controls (P < 0.01).CONCLUSION:shRNA, by targeting hTERT mRNA, has no noticeable influence on growth and proliferation of hMSCs, and might be safe for the somatic cells which are normal but do not express hTERT.
5.Molecular pathology of multifocal prostate cancer and its clinical application.
Liang CHENG ; Xiao-dong TENG ; Jie ZHOU
Chinese Journal of Pathology 2011;40(7):436-439
Aneuploidy
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Gene Rearrangement
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Glutathione S-Transferase pi
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metabolism
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Humans
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Male
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Methylation
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Neoplasm Grading
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Neoplasm Staging
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Neoplasms, Multiple Primary
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genetics
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metabolism
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pathology
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surgery
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Oncogene Proteins, Fusion
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genetics
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Prostate-Specific Antigen
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metabolism
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Prostatic Intraepithelial Neoplasia
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genetics
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pathology
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Prostatic Neoplasms
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genetics
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metabolism
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pathology
;
surgery
6.Dissecting functional chemome of Xiaoqinglong decoction analogous formulae using network formulaology approach.
Xiao-hui FAN ; Shun XIAO ; Ni AI ; Jie LIAO ; Yi-yu CHENG
China Journal of Chinese Materia Medica 2015;40(13):2634-2638
Analogous formulae (AF) refer to a set of traditional Chinese medicine (TCM) formulae sharing similar herbs and/or indications. Dissecting functional chemome of analogous formulae could enhance the understanding of the intrinsic nature of TCM. In this study, taking 5 Xiaoqinglong decoction analogous formulae (XQL AF) including Xiaoqinglong decoction, Mahuang Xingren Shigao Gancao decoction, Mahuang Fuzi decoction, Houpu Mahuang decoction and Daqinglong decoction as example, we systematically investigated the relationship between compounds and indications using network formulaology approach. The functional chemome of XQL AF were revealed by network analysis and molecular docking. This successful application in XQL AF suggests network formulaology could be a useful tool for AF-related research and therefore provide a new way to discover the scientific foundation of Zhang Zhongjing's herbal formulae.
Chemistry, Pharmaceutical
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Drugs, Chinese Herbal
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analysis
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Medicine, Chinese Traditional
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Molecular Docking Simulation
7.Age determination by radiographic feature of thyroid cartilage in male Chinese Han popullation.
Jie CHENG ; Xiao-dong ZHAO ; Xiao-qing SU
Journal of Forensic Medicine 2003;19(1):16-21
OBJECTIVE:
To seek a exact method of age determination.
METHODS:
This sample consists of 73 male Chinese han individuals whose ages have been known. Eight positions were selected from their radiograms which have been divided into three or six grade and then set up a mathematical model to deduce age by multiple linear regression analysis.
RESULTS:
The standard deviation of male thyroid cartilage is 1.854 and the multiple correctation coefficient is 0.9971.
CONCLUSION
The observation proved that age determination by radiographic feature of thyroid cartiage may be suitable for male Chinese han population from 13 to 67 years old.
Adolescent
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Adult
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Age Determination by Skeleton/methods*
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Aged
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Asian People
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Humans
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Male
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Middle Aged
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Reference Values
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Thyroid Cartilage/diagnostic imaging*
8.Characteristics of viral shedding in people infected with SARS-CoV-2 during difference stages
CHEN Xi ; ZHANG Yi-cheng ; ZHANG Jie ; ZHOU Min ; HE Qing ; LUO Jie ; XIAO Chong-kun ; ZHANG Zheng-dong
China Tropical Medicine 2023;23(3):310-
Abstract: Viral shedding of SARS-CoV-2 is a continuous dynamic process, which can be divided into latent stage, initial stage, peak stage and decreasing stage according to the characteristics of viral shedding. After being infected with SARS-CoV-2, the infected person generally stays in the latent period for 1-3 days, which is characterized by continuous negative nucleic acid test results and no infectiousness, and the risk of infection for close contacts is very low. At the initial stage of viral shedding is characterized by a rapid decline in the Ct value of nucleic acid tests in a short time, and clinical symptoms gradually appear. The infectiousness of the infected person gradually increases during this period, and the risk of infection for close contacts also gradually increases, but it is still in the early stage of infection, the possibility of viral shedding is low, and the risk of infection of secondary close contacts is low. The peak of viral shedding is characterized by low Ct value in nucleic acid test and obvious clinical symptoms; during this period, the infected person is the most infectious, and the risk of infection of the contact is the highest, so the scope of close contacts should be expanded appropriately. The decreasing period is characterized by the gradual increase of Ct value of nucleic acid test and the gradual disappearance of clinical symptoms; during this period, the infectiousness of the infected person gradually decreases to disappear. In an outbreak, an infected person in the decreasing phase is more likely to be an early infected person in the transmission chain. If infected individuals in the decreasing phase are found in an area without a SARS-CoV-2 epidemic, it suggests that the local outbreak epidemic has been spreading for some time and may be larger in scale. According to the characteristics of viral shedding, risk personnel can be determined more scientifically and accurately, so as to minimize the risk and reduce the waste of epidemic prevention resources.
9.Research progress on musk secretion mechanism of forest musk deer.
Hang JIE ; Xiao-Lan FENG ; Gui-Jun ZHAO ; De-Jun ZENG ; Cheng-Lu ZHANG ; Qiang CHEN
China Journal of Chinese Materia Medica 2014;39(23):4522-4525
Forest musk deer (Moschus berezovskii), a rare wild medicinal animal, is listed under the category of the state key protected wildlife list of China. Musk, secreted by the musk glands, is with high economic and medicinal value and used as precious traditional medicine in China. In order to meet the needs of musk in Chinese traditional medicine, forest musk deer farming was conducted in 1950s, but the research progress on musk secretion mechanism was slow. Therefore, by reviewing the histological and anatomical structure of forest musk deer musk gland, the relationship between sex hormones and the musk secretion process, and the molecular mechanism of the musk secretion, the existing problems in investigating the musk secretion mechanism were analyzed and the development trends in this field were also discussed, in order to provide a reference for further studies on the musk secretion mechanism and improve musk production of forest musk deer.
Animals
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Deer
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metabolism
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Exocrine Glands
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anatomy & histology
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chemistry
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secretion
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Fatty Acids, Monounsaturated
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chemistry
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metabolism
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Male
10.Improvements in the establishment and evaluation of cerebral ischemia models made by thread occlusion in rats
Bin HE ; Jinsong ZHANG ; Binxia SHAO ; Jie CHENG ; Fei HUANG ; Hang XIAO ; Rang GAO ; Xiang LU
Chinese Journal of Emergency Medicine 2009;18(12):1248-1251
Objective To modify the methods of operation and establishment of cerebral ischemia rat models made by thread occlusion. Method We randomly divided 120 male SD rats into a common group (n = 50), an improvement group (n = 60) and a sham-operated group (n - 10). Rats in the common and improvement groups were made into models using the common and improvement methods separately. All models were evaluated on the basis of physical sign indices and 2,3,5-triphenyltetrazolium chloride (TTC) staining. The TTC staining results were taken as gold standards. Then, we compared the achievement ratios of the two groups, and computed the sensitivity and specificity of every physical sign index based on these standards. The χ~2 or correction χ~2 test was used to compare the ratios. Results (1) The achievement ratio in the improvement group was significantly higher than that in the common group (71.67% vs. 52.00%, P = 0.034). (2) The sensitivity of evaluation for both common and improvement methods was 98.55%. However, the specificity of evaluation for the improvement method was significantly higher than that for the common method (100.00% vs. 40.00%, P =0.000). Conclusions The establishment achievement ratio and evaluation correctness of models are obviously elevated by modification of the operation and establishment methods.