Objective To comparatively investigate the characteristics of bone metabolism in forelimb malformation WHBE rabbits based on the OPG/RANK/RANKL system.Methods Healthy male 2-2.5-month old WHBE rabbit ( HWR) , Healthy Japanese rabbit ( HJR) and forelimb malformation WHBE rabbit ( FMWR) were used in this study and divided into 3 groups, HWR, HJR and FMWR groups, with 10 rabbits in each group.The shape of forelimb and mean gray value observed from X-ray film were examined, and the bone tissue micro-morphology was analyzed using HE staining.Re-al-time fluorescence quantitative PCR was used to determine the expression of osteoprotegerin ( OPG) and receptor activator of NF-KB ligand ( RANKL) mRNA.The expressions of OPG, receptor activator of NF-KB ( RANK) and RANKL protein in serum and bone tissue were assayed by enzyme-linked immunoassay ( ELISA) and immunohistochemistry, respectively. Results Compared with the HWR and HJR groups, rabbits in the FMWR group appeared abnormal in toutuous forelimb and thinner bone cortex.The mean gray values of X-ray in the FMWR group were lower than that in the HWR group ( P<0.05).There were significant differences between the FMWR and healthy rabbits (HWR and HJR) in the following pa-rameters:RANKL mRNA expression and RANKL/OPG mRNA ratio in the liver(P<0.05, P<0.01), serum protein expression of RANK and RANKL and RANKL/OPG ratio (P<0.05, P<0.01), and positive index of expression of RANKL protein and RANKL/OPG ratio in the bone tissue(P<0.05, P<0.01).Furthermore, the gene expression lev-els of OPG and RANKL of HWR were significantly higher than that of HJR(P<0.05, P<0.01).Conclusions The FMWR show some abnormal symptoms in bone metabolism as well as decrease of bone quality and histological changes of bone micro-structure, due to the significant increase of RANKL/OPG ratio of FMWR.The breed differences between WHBE and Japanese rabbits in RANKL mRNA expression level may be one of factors inducing limb malformation in the WHBE rabbits.

Objective Try to discover the safe and effective inhibitors of lymphangiogenesis. Methods Lymphatic endotheial cells from pig thoracic duct were isolated and cultured. The observation of specimens by LM and EM was made. The control group, endostatin and PF-4 experimental groups were set. Methods of the scraped line and MTT were used to examine their inhibitive effect on the lymphangiogenesis. Results The P value is

ysis, which had important reference values for further studying biological functions of UP Ⅱ gene and targeted therapeutic strategy for TCC of bladder.

Objective To investigate the effect of evidence-based pain nursing on postoperative pain and wound healing in hemorrhoids patients with hemorrhoid surgery. Methods A total of120 hemorrhoid cases treated from January to April 2018 in our hospital were randomly divided into observation group and control group. The control group (n = 60) received routine nursing during perioperative period, another 60 cases in the observation group were treated with evidence-based pain nursing on the basis of routine nursing. The postoperative pain and wound healing were compared between the two groups. Results The 24, 48 h pain scores as well as highest pain score of the observation group were all lower than that of the control group (P ＜ 0. 05). The pain scores of the first dressing change and the first bowel movement were lower than that of the control group (P ＜ 0. 05).The edema scores of the observation group at 24 and 48 hours after operation were lower, the wound score before discharge was lower, and the wound healing time was shorter than that of the control group (P ＜ 0. 05). Conclusion Evidence-based nursing can reduce postoperative pain degree, reduce wound edema and promote healing of hemorrhoid patients.

Objective To investigate the effect of evidence-based pain nursing on postoperative pain and wound healing in hemorrhoids patients with hemorrhoid surgery. Methods A total of120 hemorrhoid cases treated from January to April 2018 in our hospital were randomly divided into observation group and control group. The control group (n = 60) received routine nursing during perioperative period, another 60 cases in the observation group were treated with evidence-based pain nursing on the basis of routine nursing. The postoperative pain and wound healing were compared between the two groups. Results The 24, 48 h pain scores as well as highest pain score of the observation group were all lower than that of the control group (P ＜ 0. 05). The pain scores of the first dressing change and the first bowel movement were lower than that of the control group (P ＜ 0. 05).The edema scores of the observation group at 24 and 48 hours after operation were lower, the wound score before discharge was lower, and the wound healing time was shorter than that of the control group (P ＜ 0. 05). Conclusion Evidence-based nursing can reduce postoperative pain degree, reduce wound edema and promote healing of hemorrhoid patients.