Objective To assess the post-licensure safety and immunogenicity of freeze-dried live attenuated hepatitis A vaccine(H_2 strain) in order to popularize it as an immunization program vaccine.Methods Totally 5 000 healthy infants and young children aged 18-24 months were injected with single dose of freeze-dried live attenuated hepatitis A vaccine, and the adverse events of 0-14 d after vaccination were monitored. Among them, 200 cases were selected into immunogenic subgroups, and the serum hepatitis A virus(HAV) specific IgG antibody(anti-HAV IgG) was measured 8 weeks before and after immunization.Results A total of 5 000 participants completed inoculation, and 4 999 participants completed safety observation, among which, 424 cases of adverse reactions were reported, and the incidence of solicited adverse reactions was 8. 48%. The common local solicited adverse reactions were redness, tenderness, itching, rash and swelling at the inoculation site, while the systemic adverse reactions were mainly fever and anorexia. The severity was mainly grade 1-2, and no serious adverse events related to the vaccine occurred. Among 200 immunogenic subgroups, 188 participants were included in the per protocol set(PPS), the seroconversion rate of anti-HAV IgG antibody was 98. 91% [95% confidence interval(CI): 96. 11%-99. 87%], with a geometric mean titer(GMT) of 83. 67 mIU/mL(95% CI: 77. 05-90. 85 mIU/mL).Conclusion A single dose freeze-dried live attenuated hepatitis A vaccine(H_2 strain) presents good safety and immunogenicity for infants and young children aged 18-24 months.

In the paper,we will deeply research sorts of ethical problems in medical genetics and their origins through a view of the human dual evolutions,and bring forward the viewpoint that any ethical problem can be originated from the conflict between human biological evolution and cultural evolution.Based on fully reasoning the relation between ethical problems in medical genetics and human evolution,the authors put forward a series of corresponding strategies to solve the ethical problems in medical genetics.

Objective To establish immortalized bone marrow mesenchymal stem cells (BMSCs) from SD rats in vitro for further research on the characteristics and clinical application of BMSCs.Methods By using L ipo-fectamine TM 2000 -mediated gene transfection, plasmid pCMVSV40T/PUR containing the simian virus 40 large T antigene gene (SV40Tag) was transfected into BMSCs.BMSCs were screened by puromycin and then cultured on an extended scale.Their cell morphology and growth conditions were observed.Growth curve of cells was graphed.The expression of SV40Tag in transfected cells was identified by enzyme digestion method for the detection of tumor forma-tion.Results The growth rate of the experimental group of BMSCs after the 7th generation was significantly higher than that of the control group.No tumorigenicity was found in BMSCs after the 7th generation.Conclusion In vitro, the immortalized BMSCs by pCMVSV40T/PUR can provide basis on a large scale for its application in clinic and sci-entific research.

Objective To explore the changes of the internal structure and research topics of oncology from 2005 to 2014,and offer reference for the development of the related studies and research policies. Methods The articles published in 203 journals in the area of oncologyfrom 2005 to 2014 included by JCR were chosen.Firstly,we cleaned the author keywords using the TDA software.After that,we standardized and categorized the keywords according to the Medical Subject Headings (MeSH)manually.In the end,we builted the co-occurrence matrixes of high-frequency words in three stages:2005—2007,2008—2010 and 2011 —2014, and drew the evolution maps and strategic diagrams of each stage to analyze the changes of the internal structure and research topics of oncology.Results While the word frequence increasing rapidly,the main keywords reduced gradually from 2005 to 2014.The traditional treatments such as chemotherapy and radiotherapy,the diagnostic and the epidemiological investigation were the core subject of oncology.With the screening of neoplasms,the research on the cell physiological phenomena and the genetic phenomena gained vigorous expan-sion.Breast neoplasms,the topic whose deep exploration also led the development of other topics,has always been the hard nut as well as the hot topic of the oncology.During this period,researches on the screening of neo-plasms and the genetic phenomena were developed rapidly.Conclusion The content of oncology will be more thoroughly with some merge,division,development,declining and perishing of the internal themes occurred as well.The boundaries among the sub-structure are becoming more and more blurred and the screening of neo-plasms and the genetic phenomena has gradually become the dominant trend in general practices.

Angelman syndrome is a neurodevelopmental disorder characterized by developmental delay, intellectual disability, dyskinesia, speech impairment, pleasant expression, epilepsy and abnormal electroencephalogram.The early symptoms of this disease are not typical, and attention should be paid to identification.In this article, the recent advances in clinical, ubiquitin-protein ligase E3A gene, genetic characteristics, genetic counseling and the treatment strategies of Angelman syndrome will be reviewed.

Child ; Glucocorticoids ; therapeutic use ; Histamine Antagonists ; therapeutic use ; Humans ; Rhinitis, Allergic, Perennial ; drug therapy

Objective Anastomotic leakage is a serious complication after esophagectomy , we try to observe the early diag-nosis and treatment of intra-thoracic anastomotic leakage following esophagectomy through placing an esophageal bed drainage tube with low negative pressure on , and to explore the clinical value of esophageal bed drainage tube with low negative pressure . Methods We retrospectively analyzed the clinical data of 216 patients with esophageal carcinoma receiving intra-thoracic esophagogastro-anasto-mosis in our hospital from Mar .2013 to Nov.2015 .There were 124 patients were placed an esophageal bed drainage tube with low nega-tive pressure and a closed thoracic drainage tube after surgery ( low negative pressure group ) , and 92 patients were just place a closed thoracic drainange tube ( control group ) .Compared the differences of the incidence rate of intra-thoracic anastomotic leakage , the time of confirmed diagnosis and the time of recovery after anastomotic leakage ( time from confirmed diagnosis to recover oral intake ) between the two groups. Results There was no difference in incidences of anastomotic leakage in two groups (5.65%vs 4.34%, P>0.05), however, diagnosis of anastomotic leakage in experiment group was earlier than that of control group ([5.43±1.62]d vs [8.25±2.22]d, P<0.05) and the period of time for recovery after anastomotic leakage was shorter than control group ([29.29±10.18]d vs [78.50±20.27] d, P<0.05). Conclusion Placing a esophageal bed drainage tube with low negtive pressure in esophagectomy could be valuable for early diagnosis and treatment of anastomotic leakage as well as shorting the recovery course of anastomotic leakage after esophagectomy .

Physical beauty does not only equal to the shape of body fit for the formal principles of aesthetics, but also the embodiment of culture, and its essence lies in body which embodies wisdom. So plastic surgery does not equal to shape body according to the formal principles of aesthetics, it has to be directed by somaesthetics. Prudence in dealing with the cultural and time-varying differences of physical beauty embodies and even creates the culture of physical beauty.

OBJECTIVE: To study the cutaneous toxicity of Compound p-cymene cream.METHODS: Different dosage of Compound p-cymene cream was applied on normal skin and the damaged skin of hairless area of rabbits,and the presence of acute toxicity.RESULTS: Compound p-cymene cream induced neither acute toxicity nor cutaneous irritant reaction.CONCLUSION: Compound p-cymene cream is a safe locally applied external preparation.

Based on the historic research on the concepts of production and creation,the paper reveals the theoretical logic and inherent contradictions of the Fabricated Man.Then it proves the moral philosophy foundation of fabricating man by "physical transformation" with the study of historic evolvement of the rational creation.Finally,it proves the possible crisis in the practice of human gene technique by discussing the antinomy of "fabricating campaign".