Objective To explore the changes of the internal structure and research topics of oncology from 2005 to 2014,and offer reference for the development of the related studies and research policies. Methods The articles published in 203 journals in the area of oncologyfrom 2005 to 2014 included by JCR were chosen.Firstly,we cleaned the author keywords using the TDA software.After that,we standardized and categorized the keywords according to the Medical Subject Headings (MeSH)manually.In the end,we builted the co-occurrence matrixes of high-frequency words in three stages:2005—2007,2008—2010 and 2011 —2014, and drew the evolution maps and strategic diagrams of each stage to analyze the changes of the internal structure and research topics of oncology.Results While the word frequence increasing rapidly,the main keywords reduced gradually from 2005 to 2014.The traditional treatments such as chemotherapy and radiotherapy,the diagnostic and the epidemiological investigation were the core subject of oncology.With the screening of neoplasms,the research on the cell physiological phenomena and the genetic phenomena gained vigorous expan-sion.Breast neoplasms,the topic whose deep exploration also led the development of other topics,has always been the hard nut as well as the hot topic of the oncology.During this period,researches on the screening of neo-plasms and the genetic phenomena were developed rapidly.Conclusion The content of oncology will be more thoroughly with some merge,division,development,declining and perishing of the internal themes occurred as well.The boundaries among the sub-structure are becoming more and more blurred and the screening of neo-plasms and the genetic phenomena has gradually become the dominant trend in general practices.

In the paper,we will deeply research sorts of ethical problems in medical genetics and their origins through a view of the human dual evolutions,and bring forward the viewpoint that any ethical problem can be originated from the conflict between human biological evolution and cultural evolution.Based on fully reasoning the relation between ethical problems in medical genetics and human evolution,the authors put forward a series of corresponding strategies to solve the ethical problems in medical genetics.

Objective To establish immortalized bone marrow mesenchymal stem cells (BMSCs) from SD rats in vitro for further research on the characteristics and clinical application of BMSCs.Methods By using L ipo-fectamine TM 2000 -mediated gene transfection, plasmid pCMVSV40T/PUR containing the simian virus 40 large T antigene gene (SV40Tag) was transfected into BMSCs.BMSCs were screened by puromycin and then cultured on an extended scale.Their cell morphology and growth conditions were observed.Growth curve of cells was graphed.The expression of SV40Tag in transfected cells was identified by enzyme digestion method for the detection of tumor forma-tion.Results The growth rate of the experimental group of BMSCs after the 7th generation was significantly higher than that of the control group.No tumorigenicity was found in BMSCs after the 7th generation.Conclusion In vitro, the immortalized BMSCs by pCMVSV40T/PUR can provide basis on a large scale for its application in clinic and sci-entific research.

Angelman syndrome is a neurodevelopmental disorder characterized by developmental delay, intellectual disability, dyskinesia, speech impairment, pleasant expression, epilepsy and abnormal electroencephalogram.The early symptoms of this disease are not typical, and attention should be paid to identification.In this article, the recent advances in clinical, ubiquitin-protein ligase E3A gene, genetic characteristics, genetic counseling and the treatment strategies of Angelman syndrome will be reviewed.

Glycotechnology is a new branch of biotechnology, emerged early 1990's. In this article, the international background of glycotechnology is briefly introduced and history of glycotechnology in China is reviewed.
Biotechnology ; China ; Glycomics ; history ; History, 20th Century ; History, 21st Century

China ; Humans ; Practice Guidelines as Topic ; Rhinitis, Allergic ; diagnosis ; therapy

Objective To explore the effect of aconitine on serum zinc finger protein 41 and secreted protein acidic and rich in cysteine in ventricular septal defect model rats.Methods 80 ventricular septal defect model rats were randomly divided into two groups, experimental group ( n=40) were treated with 0.05 mg/kg aconitine via tail vein injection for 7 consecutive days;control group (n=40) were treated with normal saline via tail vein injection for 7 consecutive days.Then, the abdominal aorta blood of each rat was collected, and the contents of zinc finger protein 41 and SPARC in two groups were detected by Western blot and ELISA method,seperately.Results Western blot results showed that the expression of zinc finger protein 41 and SPARC in serum samples of experimental group were significantly lower than that of control group ( P<0.05 ) .ELISA results showed that the contents of zinc finger protein 41 and SPARC of experimental group was significantly lower than that of control group ( P<0.05 ), respectively.Conclusion Aconitine can decrease the expression of serum zinc finger protein 41 and SPARC in ventricular septal defect model rat.

Objective To analyze distribution of human papilloma virus(HPV) subtypes in women infected by HPV in Guigang area ,and to determine the dominant type in this region .Methods 21 genital HPV genotypes were screened in 13 156 women in out‐patient clinic in Guigang area from July 2013 to July 2014 by using rapid flow‐through hybridization technique of nucleic acid mole‐cules ,and high‐risk HPV detection and typing was carried out for screening cervical cancer .Results A total of 4 682 HPV‐positive women were identified and the infection rate was 35 .59% .All of 21 genetypes were checked out ,dominated by single infection .The percentages of HPV genotypes from high to low was HPV‐52 (16 .28% ) ,HPV‐16 (14 .01% ) ,HPV‐58 (8 .48% ) ,HPV8304 (7 .58% ) ,HPV‐6(6 .79% )and HPV‐11(5 .72% ) .Conclusion The dominant genetype of HPV of women in this area is HPV‐52 .

Combined with practical situation of our hospital,scientific management of economic benefits is applied to medical equipment storehouse with good effect.We traced and analyzed the economic benefits of medical equipment utilization,strengthened the supervision power,and formed a suitable management model for medical equipment in our hospital.

Infectious diseases are characterized as their pathogens.So the detection of pathogen is very important for the diagnosis of infectious diseases.Molecular diagnosis based on polymerase chain reaction (PCR) is playing an important role in the diagnosis,therapy,and prevention for infectious diseases.The concept of quasispecies must be put into consideration for quasispecies are universal for almost every pathogen and important for the diagnosis.Resistance is emerging along with anti-microbial therapy and has comprehensive impaet for treatment of infectious diseases.Diagnosis of resistance will be helpful for seleetion of right drug,and modification of therapy protocol,and reduction of resistance.Genotyping technique is meaningful for individualized therapy for patients with infectious diseases.