Objective To compare the clinical effect of different doses of oxycodone on endotracheal intubation during general anesthesia induction period.Methods Ninety-five patients with endotracheal intubation general anesthesia were selected as the research subjects,and the patients were randomly divided into group A,group B and group C.31 patients in group A received intravenous administration of oxycodone 0.20mg/kg,32 patients in group B received intravenous administration of oxycodone 0.25mg/kg,and 32 patients in group C received intravenous administration of oxycodone 0.30mg/kg.The adrenaline,norepinephrine,dopamine concentration,as well as mean arterial pressure and heart rate at different time points were compared among the three groups.Results The mean arterial pressure and heart rate at T0 time point were decreased to varying extent compared with those at T1 time point(t =23.618,7.412;22.928,9.063;32.636,9.422,P =0.000,0.000;0.000,0.000;0.000,0.000).The heart rate,mean arterial pressure at T2,T3 and T4 time points in group A were increased compared with T0 time point (t =13.834,6.506,7.015;17.604,14.796,16.323,P =0.000,0.000,0.000;0.000,0.000,0.000).The adrenaline,norepinephrine and dopamine at T2,T3 and T4 time points in group A were increased compared witb T0 time point (t =121.662,64.533,15.687;31.498,16.933,9.286;39.712,116.049,147.102,P =0.000,0.000,0.000;0.000,0.000,0.000;0.000,0.000,0.000).The heart rate,mean arterial pressure,adrenaline,norepinephrine and dopamine at T2,T3 and T4 time points in group B and group C were decreased significantly compared with those in group A (t =19.586,31.972,16.844,27.087;14.046,14.822,15.377,23.826;7.756,14.396,13.268,23.342,100.573,1 04.010,37.612,38.892,107.377,117.969;56.135,56.757,24.223,25.300,95.057,82.088;22.878,25.731,5.532,5.934,134.763,83.628,P =0.000,0.000,0.000,0.000,0.000,0.000;0.000,0.000,0.000,0.000,0.000,0.000;0.000,0.000,0.000,0.000,0.000,0.000;0.000,0.000,0.000,0.000;0.000,0.000,0.000,0.000;0.000,0.000,0.000,0.000),and the differences were statistically significant (all P ＜ 0.05).Conclusion During the induction of general anesthesia,the appropriate dose of oxycodone in patients with endotracheal intubation was 0.25mg/kg,which could effectively maintain the hemodynamic stability,such as mean arterial pressure,and could reduce the stress response.

Objective To investigate the relationship between plasma P substance(SP),atrial natriuretic peptide(ANP) and renal function in chronic renal failure(CRF).Methods SP and ANP were measured by radioi m munoassay in normal control and CRF before and after hemodialysis(HD),serum creatinine(Cr) was determined simultaneously.Results SP before HD was significantly higher than that of control(P

Objective To evaluate the effect of oral levothyroxine plus intrathyroidal injection of dexamethasone on conversion of subclinical hypothroidism caused by Hashimoto's thyroiditis.Methods 80 subjects with Hashimoto thyroiditis were randomly divided into 2 groups.Subjects in levothyroxine group(40 cases) were treated with oral levothyroxine sodium,subjects in dexamethasone group(40 cases) were treated with oral levothyroxine plus intrathyroidal injection of dexamethasone.Serum free triiodothyronine(FT_3),free thyroxine(FT_4),sentient thyrotropin(sTSH) and other parameters were evaluated before treatment and after treatment in patients with Hashimoto's thyroiditis.Results The content of FT_3,FT_4 after treatment were obviously higher than that before treatment(t=2.123,P

Objective To study the status of telomerase activity during the development of thyroid tumors,and to determine whether telomerase activity can be used clinically as a molecular marker in the differential diagnosis of thyroid cancer.Methods Telomerase activity was measured in 37 thyroid carcinomas,33 benign thyroid lesions and 30 normal thyroid tissue samples by means of telomerase repeat amplification procedure-polymerase chain reaction(TRAP-PCR).The assay was also applied to 15 fine needle aspirates(FNAs) of thyroid carcinomas to test its sensitivity.Results Thirty-one of 37 thyroid carcinomas(83.8%),7 of 33 benign thyroid lesions(21.2%),and 4 of 30 adjacent normal thyroid tissue asmples expressed telomerase activity,15 FNAs also had positive telomerase activity,just as their corresponding tissue specimens.The quantitative analysis showed that the telomerase activity was significantly higher in thyroid carcinomas than that in benign thyroid tissue samples.And medullary carcinomas and anaplastic carcinomas had higher levels of telomerase activity than papillary carcinomas.Conclusion Telomerase activity is a good marker for thyroid carcinomas.The quantitative TRAP-PCR might have more potential application in the differential diagnosis of tumors and the estimation of tumor progression and prognosis.And this sensitive assay could become a useful new modality for supplementing microscopic cytopathology in the detection of cancer cells in small tissue samples and FNAs.

Drag-loaded nanoparticle is a new delivery cartier of drug and biomacromoleculc,which have many fortes such as target ability,permeability,controlled release ability and function of promoting cell differentiation.Polypeptide combined nanoparticles can deliver drugs to the targeted tissues by conjugating with specific ligands.Growth factor combined nanoparticles may have better curative effect by maintaining the activity of the growth factors longer.Nanoparticles can effectively deliver genes to the cells in the injured tissue for the enhancemem of the specific function of the cells.Three dimension nanometer fibrous structure combined with nanoparticle based drug delivery could significantly promote the growth of the cell.Magnetic nanoparticles are widely used for the targeted delivery of drugs or genes and separation of biomolecules.Drug loaded nanoparticles have been increasinsly used in tissue enginerring which have shown a tremendous potential.In the present paper,we reviewed the application of drug-loaded nanoparticles in the field of tissue engineering.

Objective To investigate the diagnostic and therapeutic methods for liver injury.Methods The clinical data of 80 patients with liver injury were retrospectively analyzed.Results The liver injury was srede Ⅰ in 12 patients.grade Ⅱ in 25 patients,grade Ⅲ in 20 patients,srede Ⅳ in 14 patients,and grade V in 9 cases.16 patients were not treated with operation,24 patients were treated with hepatic artery ligation,and 3 patients were treated with plugging.75 patients were cured,and 5 patients died.Conclusion Liver injury should be clearly diagnosed as early as possible,correct therapeutic methods should be selected according to the state of the injury,and operative manner should be reasonably selected according to intraoperative conditions.The treatment of complications and postoperative protection to the function of important organs should be paid attention to.

ObjectiveTo investigate the effect of early fluid resuscitation on the prognosis of SAP patients.MethodsPatients who were admitted to our hospital within 72 h after the onset of the SAP were enrolled.The parameters for the fluid resuscitation were as follow: heart rate ＜120 beat/h,urine output ≥ 1 ml ·h-1 · kg-1,hematocrit ≤35％ and mean arterial pressure 65 ～ 85 mmHg ( 1 mmHg =0.133 kPa).The effects of different time of reaching fluid resuscitation ( ＜48 h,48 -72 h,＞72 h) and parameters achieved (0 ～ 1parameter,2 parameters,3 parameters,4 parameters) on the prognosis were analyzed.ResultsThere were 70 patient enrolled in this study and 41 ( 58.6％ ) developed complications,20 ( 28.6％ ) were referred to surgical operation and 10( 14.3％ ) died.The median hospital stay was 23.5 d,and the median medical cost was 71.9(5.7～567.4 thousands RMB).The rates of surgical intervention in ＜48 h,48 ～72 h,＞72 h groups were 20.0％,33.3％,75.0％,and the rates of acute kidney injury were 20.0％,25.0％,75.0％,while the rates of complications were 50.0％,83.3％,100％,and the difference among the 3 groups was statistically significant (P ＜ 0.05 ).The rates of surgical intervention in patients achieving 0 ～ 1,2,3,4parameters within 48h of SAP onset were 50.0％,26.3％,13.0％,25.0％,and the rates of acute kidney injury were 45.0％,31.6％,17.4％,0,while the rates of ARDS were 35.0％,31.6％,13.0％,0,which showing a significant decreasing trend.ConclusionsAn appropriate fluid resuscitation in the first 48 h after the onset of SAP was benefit for improving the treatment effects and patients' outcome.

The transmitting network is reconstructed to make the network syetem convenient,stable and swift when transmitting CT image.

OBJECTIVE To study the effect of optimal decontamination methods on the management of implantation instruments of orthopedics.METHODS To clean the instruments by the Campany(control group) and by the nurse after hospital training(management group),and compare the decontamination quality of the two methods by visual inspection using magnifying glass.RESULTS The qualification rate of instruments of the management group was more than control group(P

Objective To explore the clinical effect of drug combination psychological intervention in colorectal cancer patients.MethodsRetrospective analysis of 60 cases surgical histopathology diagnosis of colorectal cancer patients with clinical diagnosis and treatment data from January 2014 to January 2016 in the Shaoxing Second Hospital, all patients were treated with surgery, including postoperative chemotherapy, hyperthermia treatment as the control group;In the control group based on supplemented by traditional Chinese medicine Fuzheng Xiao′ai Tang, psychological intervention for the observation group, each group of 30 cases.ResultsThe scores of quality of life and self-care ability of the two groups were significantly higher than those of the control group before and after treatment, and the scores of self-care ability were significantly higher in the observation group than in the control group (P<0.05) The levels of CEA, CA199 and CA242 were significantly lower than those of the control group before and after treatment, the level of tumor markers was significantly lower than that of the control group (P<0.05).The total effective rate was 73.33% in the observation group, which was significantly higher than that in the control group (43.33%, P<0.05).The observation group was significantly higher than the control group (P<0.05) Mental health was significantly lower than the control group,(P<0.05).ConclusionChinese medicine Fuzheng Xiao′ai Tang combined with chemotherapy and psychological intervention in the treatment of colorectal cancer after the effect is significant and safe.