The zymograms of lactate dehydrogenase (LDH) and esterase (EST) isoenzymes in different physiologic state from two species of Anopheles mosquitoes were studied with polyacrylamide gel electro -phoresis. Some zymogram variation of LDH from single female mosquito was found after engorged blood meals for 48 h, that consisted of two main bands whose relative mobility (Rm) was different. The isozymograms of EST showed some similar characteristics of sibling which divided into three groups ESTⅠ, ESTⅡand ESTⅢ, respectively. The two species of mosquitoes could be differentiated from each other by the particular band in EST Ⅱ group of An. anthropophagus.

A simple and reliable procedure for the isolation of pure DNA from human fetal liver is described.A high pure and natural DNA with an average molecular weight of 4 ?106 can be easily obtained after the sample is treated with SDS and trypsin and fractionated by a sepharose 4B column.The product meets the requirement for general research work of molecular biology.

This paper is to report the results of the clinical application of the Shexiang hemostasis-removing plaster. 265 cases of dermatohemostasis including chilblain, erythema multiforme, erythema nodosum, and so on were treated locally with Shexiang hemostasis-removing plaster. 20 cases of dermatohemostasis were treated with Shexiang analgesic plaster and served as control.It was found the rates of marked improvement and improvement after the application of Shexiang hemostasis-removing plaster were 85.61% and 96.60%, while those after Shxiang analgesic plaster treatment were 65% and 90% respectively. It is bovious that Shexiang hemostasis-removing plaster is more effective in treating dermatohemostasis than Shexiang analgesic plaster. The difference between the 2 is statistically significant(P

Acute kidney injury(AKI) can lead to remote organ damage.The specific mechanism by which the AKI results in distant organ damage is yet to be determined.In animal models,it has become increasingly clear that AKI is not an isolated event but results in remote organ dysfunction involving the heart,lungs,liver,and brain.This review summarizes the effects of AKI on remote organs.

Coronal restoration of endodontically treated teeth may be viewed as one of the main parameters that influence the outcome of endodontic treatment. The purposes of restoring endodontically-treated teeth include preventing recontamination of the root canal system and periapical area, replacing the compromised dental hard tissue, restoring the coronal morphology and function, providing necessary strength for the restoration/tooth complex for functional stress, and avoiding crown and/or root fracture. This article reviewed recent researches on the restoration of endodontically treated teeth, provided evidence for clinical practice on topics as when to restore them, basic principles to be considered during treatment planning, and specific restoration options for both anterior and posterior teeth under different functional occulsal load conditions. Several issues should be taken into account during the decision making process, such as remaining tooth tissue, functional masticatory forces, comprehensive oral rehabilitation, and esthetic requirements.
Decision Making ; Dental Restoration, Permanent ; Humans ; Root Canal Therapy ; Tooth Crown ; Tooth, Nonvital

Asthma ; epidemiology ; prevention & control ; therapy ; Child ; China ; epidemiology ; Drug Therapy, Combination ; Humans ; Immunotherapy

Objective To compare the regulatory functions of Danhong and Salvia Ligustrazin on abnormal coagulation factors Ⅶ caused by ACS.Methods All cases were randomly divided into group A and B and a control group.Each group contains 60 patients.The control group was treated with conventional western therapies.On this basis,group A Was treated with Danhong injection and group B was treatedwith Salvia Ligustrazin injection.Compare the regulatory function of its group on abnormal level of coagulation factor Ⅶ caused by ACS.Results Group B showed better effects in its regulating abnormal level of coagulation factor Ⅶ as compaared to group A(χ~2=3.67,P＜0.05).Both groups did better than the control group in adjusting coagulation factors Ⅶ (P＜0.05).Conclusion Salvia ligustrazine has better effect in regulating coagulation factors Ⅶ than Dan Hong injection,which is better than conventional western medical therapy.

Objective To explore different pathogenesis of chronic hepatitis B(CHB)and asymptomatic H BV carriers(ASCs)by identifying differentially expressed genes.Methods Subtracted library was constructed by suppression subtraetive hybridization(SSH),and α-defensin was identified by dot blot hybridization.Peripheral blood was collected from 46 CHB patients and 11 ASCs.and the expressions of α-defensin mRNA in peripheral blood mononuclear cell and protein in plasma were determined by the real time RT-PCR and enzyme-linked immunosorbent assay(ELISA).Results Real time RT-PCR showed that the expression of α-defensin mRNA in blood samples of CHB was 1.4-fold higher than that of ASCs.As shown by ELISA,the plasma level of α-defensin in CHB was higher than that of ASCs [(216.40±81.25)μg/L vs.(156.00±57.26)μg/L,t=2.23,P<0.05].Conclusion α-defensin may involve in the pathogenesis of CHB,for it iS over-expressed in CHB patients.

Objective To study the effect of different therapies for posterior lumbar disc herniation.Methods A total of 8 000 patients with lumbar disc posterior surgery were enrolled in this research at our hospital.The technical points of the patients in different times were analysed.The results of the operation were observed.Results From January 1980 to December 1990,patients underwent laminectomy.From January 1991 to December 2000,patients were treated with laminectomy and semi-lamina.From January 2001 to December 2016,the open window accounted for 86.0％,expanding the window or semi-lamina accounted for 11.0％,and the whole laminar plate only accounted for 3.0％.After 2000,the accuracy of the diagnosis was 98.0％,the excellent rate of operation was 99.8％,the average operation time was(80±15)min,and the blood loss was(100±20)mL.Among them,the rate of I/A healing was 99.8％,dural tear rate was 4.0％,nerve root injury rate was 0.5％,and cerebrospinal fluid leakage rate was 0.9％.The incidence of complications in the cases after 2000 was significantly lower.Conclusion By summarizing the operation technique of posterior lumbar disc herniation,the operation error can be avoided and the incidence of complications of surgical injury can be reduced.

Objective To optimize and establish the extraction of total flavones in Lonicera japonica. Methods The ultraviolet spectrophotometry was used to determinate the total flavonoids. The effect of extraction time, the amount of solvent and extraction times,pH were tested by the orthogonal design.ResultsThe total flavonoids showed a linear relationship,the optimal extraction condition of total flavones in Lonicera japonica was with 16 times volume of sample weight water for three times, each time was 2 h.Conclusions The method is simple and applicable to the extraction of total flavones in Lonicera japonica.