Objective To analyze the characteristics of pulmonary function and the clinical significance of non‐specific pattern (NSP) ,and provide basis for the clinical research .Methods A total of 120 pulmonary function tests of adult patients from January 2013 to June 2014 were selected and divided into NSP group(n=20) and normal lung function group(n=100) according to their lung function behavior .The pulmonary function test results ,clinical diagnosis and radiological manifestations were analyzed .The relevant factors affecting NSP occurred were selected and logistic regression analysis was used to analyze multiple factors .Results The FEVl ,VC ,TLC ,FVC ,MVV and FEVl/FVC in normal group were significantly higher than that of NSP group and the RV/TCL was lower than NSP group ,the difference was statistically significant (P<0 .05) .Patients in NSP group were with obstructive and (or) restrictive clinical manifestations .There were 9 patients were without lung disease .Univariate analysis showed that age , BMI ,smoking history ,smoking ,obstructive diseases ,and restrictive disease in NSP group were significantly higher than that in nor‐mal group ,the difference were statistically significant (P<0 .05) .Multivariate analysis showed that age ,BMI ,history of smoking , obstructive diseases and restrictive disease were independent risk factors for NSP (P<0 .05) .Conclusion Old age ,smoking and o‐besity could increase the incidence rate of NSP .The clinical characteristics include obstructive and restrictive ventilation defects ,and need to be identified positively .

Objective:To analyze the demands of maintenance management system of medical equipment, and realize the scientization and normalization of plan design for maintenance management system of medical equipment.Methods: On the basis of current status of maintenance management system of medical equipment, the demands of various department during management process were analyzed, and then a systematic maintenance management plan was designed to face to all of medical equipment in hospital.Results: The preponderances of this system were discussed from three aspects: equipment maintenance, equipment archive and equipment benefit.Conclusion: The application of this system can accelerate the progress of maintenance for medical equipment, enhance maintenance awareness of clinical medical staffs for medical equipment, improve the transparency of equipment scrapping and disposal, and provide effective references for demonstration and purchase of medical equipment.

In order to evaluate the applied value of soluble intracellular adhesion molecule-1 (sICAM-1) in acute rejection (AR) following liver transplantation, the expression of sICAM-1 protein was sequentially detected by using ELISA in serum and bile of 43 patients receiving liver transplantation. In AR group, the expression levels of sICAM-1 protein were increased 3 days before and immediately on the establishment of AR diagnosis, and there was significant difference in the expression of bile between AR group and control group (P<0.01). After reversion of AR with hormone intensive therapy, there was significant difference in the sICAM-1 protein expression of serum and bile between AR group and control group. It was concluded that the sequential detection of sICAM-1 protein level in serum and bile was a reliable and noninvasive method for the early diagnosis of AR after liver transplantation and was valuable to observe the curative effects of anti-AR therapy.

Objective:To investigate the role of anaphylatoxin C 5 a in patients with asthma .Methods:A prospective study was performed between September 2006 and February 2007.A total of 33 patients with acute exacerbation of asthma and 13 healthy subjects were recruited into the study .The patients with acute exacerbation of asthma were also studied when they returned to the remission state .Levels of lung function, levels of C5a in induced sputum and cell differential count in induced sputum were determined . Results:The level of C5 a in induced sputum was significantly higher in patients with acute exacerbation of asthma [0.85(0.68-2.13) μg/L] than that in patients with stable asthma [0.45(0.26-0.88)μg/L, Z=-2.193, P=0.013];Sputum C5a levels in stable asthma patients were significantly higher than those in healthy controls [0.14(0.06-0.45) μg/L, Z=-2.141, P=0.015].The level of C5a in patients with severe exacerbation [2.21(1.27 -9.0) μg/L] was significantly higher than those in patients with mild exacerbation [0.34(0.17-0.63) μg/L] and moderate exacerbation [0.85(0.55-1.67) μg/L,χ2 =12.330, P=0.001].The level of C5a in induced sputum was positively correlated with the number of total cells count (r=0.797, P=0.004), neutrophils (r=0.504, P=0.032) and macrophages ( r =0.424, P=0.036 ) in acute exacerbation of asthma .Conclusion: C5a levels in induced sputum could be identified as an important prognostic biomarker , which involved in asthma ’ s pathogenesis .

Objective:To investigate the role of endogenous hydrogen sulfide(H2S)in patients with asthma.Methods:Levels of serum H2S,lung function and cell differential count in induced sputum were studied in 44 patients with acute exacerbation of asthma,33 patients with stable asthma and 12 health subjects.Of the 33 patients with stable asthma,3 failed to achieve induced sputum.Results:The serum H2S level was(75.2?13.0)?mol/L in controls(12 cases),(55.8?13.6)?mol/L in patients with stable asthma(33 cases),(57.8?6.3)?mol/L in patients with mild of acute exacerbation asthma(9 cases),(40.8?5.1)?mol/L in that with moderate of acute exacerbation asthma(13 cases),and(31.3?2.9)?mol/L in that with severe of acute exacerbation asthma(22 cases,F=44.592,P

Objective: To determine the role of cross-talk between calcineurin-dependent signal transduction pathway and protein kinase C (PKC), mitogen-activated protein kinase (MAPK) and protein kinase A (PKA) in airway remodeling in asthma. Methods: Male guinea pigs were sensitized with intraperitoneal injections of ovabumin (OVA), then treated with cyclosporin A (CsA,5 mg/kg), an inhibitor of calcineurin, then inhaled OVA for 2 weeks 14 days later. Activities of calcineurin, PKC, MAPK, and PKA were was analyzed by phosphorylation and dephosphorylation. In primary cultures of rat airway smooth muscle cells (ASMC), activities of calcineurin, PKC, MAPK, and cross-talk induced by urotensin Ⅱ (UⅡ), a recently identified strong mitogen, were measured. Results: (1) The activities of calcineurin, MAPK and PKC increased by 19% (P0.05). (4) CsA 10 -6 mol/L inhibited UⅡ-stimulated PKC activity by 14% (P0.05). Conclusion:The signal transduction pathways between calcineurin and other protein kinases such as PKC, MAPK and PKA have cross-talk in airway remodeling in asthma.

Objective:To investigate the changes and clinical implications of serum procalcitonin in exacerbation of chronic obstructive pulmonary disease(COPD).Methods:We have evaluated PCT measurement in 45 patients with an exacerbation of COPD(group A) and 25 patients with stable COPD(group B),quantitative sputum culture was performed,too.PPMs were only regarded as significant if they reached a growth of ≥10~7cfu/mL,indicating the presence of bacterial infection.Results:In patients with an exacerbation,15 patients,sputum yielded a high(≥10~7cfu/mL) bacterial load(group A_1),30 patients,sputum yielded a low(0.05).Using a cut-off point of 0.155?g/L for PCT,the sensitivities and specificities for bacterial infection in patients with an exacerbation of COPD were 93.3% and 60% respectively.Conclusion: Serum procalcitonin measurements in patients of an exacerbation of chronic obstructive pulmonary disease play a role in the diagnosis of bacterial infection.

Drugs, Chinese Herbal ; Humans ; Rhabdomyolysis

A rapid and simple flow-injection-catalytic spectrophotometric method using stopped-flow technique for determination of trace phenols is described. This methods is based on the catalytic effect of phenols on the reaction of rhodamine B and potassium bromate in sulfuric acid medium. The optimal experimental conditions are obtained by using the controlled and weighed centroid simplex method. The linear range of the determination is 0.60～4．00 mg/L for phenols, and the detection limit is 0.1 mg/L with the sample throughput of 34/h. The relative standard deviation of eleven replicate measurements is less than 2.0%. It has been applied for the determiantion of phenols in waste water with satisfactory results

A rapid and sensitive flow-injection spectrophotometric method was proposed for the determination of metoclopramide. This method was based on the diazotisation of metoclopramide with sodium nitrite in acidic medium and coupling with α-naphthylamine. The optimal experimental condition was obtained by using the simplex optimization method. The linear range was 0.0 ～ 30 mg/L, and the sample throughput was 160 samples h-1. The relative standard deviation of eleven replicate measurements was less than 0.6%. The method was used to determine metoclopramide in tablets and injections. The results were statistically compared with those obtained by pharmacopoeia method.