Objective: To investigate the expression ofVEGF-Aand the correlation with the sentinel lymph nodes lymphangiogenesis and its metastasis in non-small cell lung cance(rNSCLC).Methods: The expression of VEGF-A was detected in 65 patients with NSCLC by SP immunohistochemistay. The expression of LYVE-1 was detected by immunofluorescence. The new lymphatic vessel density was counted by LYVE-1 and analyzed with clinicopathologic parameters. Results: The positive rate of VEGF-A was 72.3%(47/65),which was positively correlated with sentinel lympha nodes metastasis(r=0.453,P

0.05).Conclusion:QDs show no effect on cytobiological behavior of living MG-63 cells.

Objective To observe clinical effect of treating helicobacter pylori infection with self-designed gastritis granule. Methods 37 patients of Helicobacter Pylori (Hp) infection with chronic gastritis accompanied by gastric mucosa atrophy and erosion were randomly recurited into a control group and a treatment group. The control group was treated with PPI trigeminy therapy, and the treatment group was treated with self-designed gastritis granule combined with PPI trigeminy therapy. Both groups were treated for continuous four weeks. Results The eradication rate of Hp in treatment group was significantly higher than that of the control group (P<0.05) .Conclusion The treatment of self-designed gastritis granule on chronic gastritis accompanied by gastric mucosa atrophy and erosion has effective results.

OBJECTIVE:To evaluate the utilization of blood lipid-lowering drugs in Haikou area.METHODS:The consumption sum,DDDs and DDC of blood lipid-lowering drugs in 2 hospitals of Haikou area from 2007 to 2009 were analyzed retrospectively.RESULTS:The consumption sum of blood lipid-lowering drugs in Haikou area increased year by year,up by 41.25% in 2008 than in 2007 and 28.96% in 2009 than in 2008.Blood lipid-lowering drugs were mainly atorvastatin,pravastatin,fluvastatin,simvastatin,bezafibrate.Atorvastatin took up the first place of the list of consumption sum and DDDs in past 3 years.CONCLUSION:Statins occupy the predominate place among blood lipid-lowering drugs and show potential in the market.

Periprosthetic joint infection (PJI) is a devastating complication after total joint arthroplasty.Currently,PJI is the main cause for knee revision and the third reason for hip revision.Accurate diagnosis is of quite importance for patients with potential in revision surgery.However,there is no test alone can achieve both perfect sensitivity and specificity in diagnosis of PJI.The following diagnostic methods still have irreplaceable role,including erythrocyte sedimentation rate (ESR),C reactive protein (CRP) test,synovial white blood cell count and polymorphonuclear percentage,bacteria culture and histology analysis.Some novel diagnostic techniques,such as leucocytes esterase strip test,show advantages in diagnosing as fast,convenient and cost-saving.Synovial biomarkers,such as α-defensin,have promising prospect with both high sensitivity and specificity.Among various diagnostic methods,bacteria culture and drug sensitivity test can guide the therapy of PJI.Thus,it is crucial to improve the positive rate of bacteria culture.With the development of technology,sonication and polymerase chain reaction (PCR) will be the future research highlights.Although the imaging technologies,such as labeled leukocyte imaging and 18F-fluorodeoxyglucose positron emission tomography (18F-FDG PET),have been progressed in the past years,most of them are still in controversy.Further researches are still needed in the future.

The experiments were designed to study the relation between endotoxamia and renal insufficiency and the protective effects of RSM on galactosamine-induced acute hepatic failure. The results indicated that galactosamine-treated rats which all accompanied with endotoxamia exhibited significant renal dysfunction (Per, BUN elevated; UV, Ccr, U_(Na)V fell; FE_(H2O), FE_(Na) enhanced). After treatment of RSM, the endotoxemia was reduced while renal functions were improved. It suggested that there was a close relationship between endotoxemia and renal insufficiency. The experiment proved that the endotoxin was an important factor in the occurance and development of HRS. RSM possessed marked preventive and theraputic effects for HRS. In addition, the model of galactosamine-induced hepatic failure could be used to study the pathogenesis, prevention and therapy of HRS simultaneously.

Objective To analyze the effect of Kangfuxin Liquidon on gingiva groove liquid interleukin 1β(IL-1β), prostaglandin E2 (PGE2), soluble adhesion molecule-1 (sICAM)in fixed orthodontic patients with gingivitis. Methods 96 cases of patients with fixed orthodontic gingivitis consult the draw method were divided into control group and experimental group, 48 cases in each group. The control group were treatedby gums clean, experimental group based on the control group were treattedby Kangfuxin Liquidon. The IL-1β, PGE2, sICAM-1 levels, periodontal status, the grade of swelling and pain, the clinical curative effect were compared between two groups. Results After treatment, the IL-1β, PGE2, sICAM levels of experimental group were lower than the control group (10.54±1.41) ng/L vs.(11.85±1.71)ng/L, (284.62±35.21) ng/L vs.(314.65±39.48)ng/L, (150.49±18.11) μg/L vs.(162.83±20.26) μg/L,the differences were statistically significant (P<0.05).The periodontal status, swelling and pain grading ofexperimental group were better than control group (P<0.05). Theeffective rate of experimental group was higher than the control group (95.83%vs.79.17%) (P<0.05). Conclusion Kangfuxin Liquidon can reduce fixed orthodontic patients with gingivitis gingiva groove IL-1β, PGE2, sICAM-1 levels, improve the periodontal status, relieve swelling and pain, improve the clinical curative effect.

Recurrence after surgery for common bile duct stones has not been solved properly,and recurrence of calculi often requires reoperation,which brings patients great pain and economic burden.Various risk factors may mediate the recurrence of common bile duct stones,including biliary tract infection,bile duct stenosis,cholangiectasis,cholestasis,Oddi sphincter dysfunction,abnormal bile composition,and surgical procedure.These risk factors are analyzed and related preventive measures are summarized.Current research findings show that prevention and treatment of risk factors for recurrence of calculi can reduce the recurrence rate of calculi and play an important role in preventing the recurrence of common bile duct stones.

OBJECTIVE:To enhance the competitive edge of Chinese pharmaceutical enterprises.METHODS:Patents problems confronted by Chinese pharmaceutical enterprises were analyzed in terms of the research and development and patent protection of the original new drugs,the administrative protection and patent protection of drugs,patent documentation in?formation,patent strategy and patent talents,etc.RESULTS&CONCLUSION:Chinese pharmaceutical enterprises should highlight the patent problems and apply patent strategy to achieve competitive advantage.

OBJECTIVE: To prepare methazolamide eye drops and establish its quality control method.METHODS: Methazolamide eye drops was prepared using inorganic nanoparticles as carrier and methazolamide as principal agent.The content of methazolamide was determined by ultraviolet spectrophotometry.The stability of the preparation was investigated. RESULTS: The preparation was white suspension andin conformity with the description ofChina Pharmacopeia(2005 edition) inidentification and test.The linear concentration range of methazolamide was4~12?g.mL-1(r=0.999 9) and the average recovery was100.10% (RSD=1.11%).Methazolamide eye drops was unstable under highlight , while all the test items had no significant change in6-month accelerated test.CONCLUSION: The preparative method is si mple and feasible and the quality of the preparationis stable and controllable.But the preparation should be stored away formlight.