Objective To compare the prevention effect of atrial fibrillation recurrence in hypertensive patients by telmisartan versus nifedipine.Methods Ninety-eight cases of hypertensive with paroxysmal atrial fibrillation patients were enrolled and divided into telmisartan group and nifedipine group with 49 cases each by random digits table method.Telmisartan group was given telmisartan 80 mg/d,nifedipine group was given nifedipine 30 mg/d.Dose of the drug needed to be adjusted according to individual condition until achieving the target.Followed-up for 18 months.Observed two groups of recurrence of atrial fibrillation and the recurrence time for the first time,recorded before and after the treatment of cardiac parameters:including left atrial diameter (LAD),left ventricular end diastolic diameter(LVDD),left ventricular posterior wall thickness (LVPWT),interventricular septum thickness (IVST),left atrial volume index(LAVI) and left ventricular mass index(LVMI).Results The blood pressure after treatment in two groups was lower than that before treatment,there was statistically significant difference (P＜ 0.05),but there was no statistically significant difference between two groups (P ＞ 0.05).After treatment,the recurrence of atrial fibrillation,atrial fibrillation recurrence time for the first time in two groups had no statistical significance (P ＞ 0.05).But the incidence of persistent atrial fibrillation in telmisartan group was significantly lower than that in nifedipine group [4.1％ (2/49) vs.16.3％ (8/49)],there was statistically significant difference (P =0.045).The cardiac parameters between two groups before treatment had no significant difference (P ＞ 0.05).After treatment,the cardiac parameters in two groups were signifcantly reduced,there were statistically significant differences (P ＜ 0.05).Moreover,the LAD,LVPWT,LAVI and LVMI in telmisartan group after treatment were significantly lower than those in nifedipine group [(37.6 ± 2.9) mm vs.(38.9 ± 2.4) mm,(11.6 ± 1.0) mm vs.(12.0 ± 0.9) mm,(22.0 ± 3.6) ml/m2 vs.(23.9 ± 4.2)ml/m2,(120.4 ± 18.2) g/m2 vs.(129.5 ± 19.2) g/m2],there were statistically significant differences (P ＜ 0.05).Conclusions Nifedipine and telmisartan can effectively lower the blood pressure.But telmisartan is superior to preventing atrial fibrillation recurrences and developing in hypertensive with paroxysmal atrial fibrillation patients,promoting cardiac parameters and postponing myocardial remodeling.

Competitive fluid phase radioimmu-noassay was conducted with eightanti-apoB-100 monoclonal antibodieswhich had been established in our laboratory.We found thatthere were at least three differenttypes of epitopes on the hydratedsurface of the lipoproteins-VLDL,IDL and LDL.These observations were of certainsignificance in studies on antigenicstructures of apoB,as well asassays for apoB in various fractionsof lipoproteins.

OBJECTIVE:To observe the clinical efficacy of Compound jianghuang decoction in preventing nonproliferate diabetic retinopathy(NPDR) from developing into proliferate diabetic retinopathy(PDR).METHODS:The clinical data of 123 patients(208 eyes) with NPDR receiving Compound jianghuang decoction(n = 64 cases,treatment group) or low dose of enteric - coated aspirin(n = 59,control group) in addition to hypoglycemic drugs administered in both groups to control blood sugar level were analyzed retrospectively.The clinical efficacy in the two groups was observed by fundus examination and fundus fluorescein angiography.RESULTS:The proportion of developing into PDR in the treatment group was significantly lower than in the control group.CONCLUSION:Compound jianghuang decoction is effective in suppressing retinal neovascularization and preventing NPDR from developing into PDR.

Cell culture medium is one of the essential raw materials in the field of life and health.In recent years,the performance and quality of domestic cell culture media have been improving,and the market share of domestic vendors has steadily increased from 19.2% in 2017 to 33.7% in 2021.However,there are also some problems and shortcomings in industrial development,mainly including:technology and process accumulation need to be strengthened;product quality need to be improved;lack of industry standards and norms.Based on literature research,special topic discussion and expert interview,this paper reviews the development history of cell culture medium,deeply analyzes and systematically combs the opportunities and challenges faced by the industry development from the basic situation,current situation and trend of the development of cell culture medium industry in China,and puts forward relevant countermeasures and suggestions.

OBJECTIVETo analyze the factors in the non-operative treatment of cervical spinal cord injury without fracture or dislocation.

METHODSThe clinical data of 122 patients with cervical spinal cord injury without fracture or dislocation from January 2009 to December 2012 treated by non-operative treatment were retrospectively reviewed. There were 84 males and 38 females, aged from 18 to 83 years with an average of (52.37 +/- 13.27) years. The clinical features, such as age, gender, cause of injury, time from injury to treatment, ASIA grade of spine cord injury, MRI type of spine cord injury, range of spine cord injury, effective cervical spinal canal ratio, Pfirrmann grade of intervertebral disc herniation, segment of intervertebral disc herniation, intervertebral disc ligament complex injury, treatment of high-dose methyiprednisolone, were observed. Single factor and multiple factor Logistic regression analysis were used in the clinical data in order to analyze the influencing factors of above items to prognosis.

RESULTSFor univariate analysis, the factors such as MRI type of spine cord injury, extent of spine cord injury, effective cervical spinal canal ratio, Pfirrmann grade of intervertebral disc herniation, segment of intervertebral disc herniation, ASIA grade of spine cord injury, associated with prognosis (P < 0.05). Multiple linear regression analysis showed that the main prognostic factors including MRI type of spine cord injury, range of spine cord injury, effective cervical spinal canal ratio, Pfirrmann grade of intervertebral disc herniation, ASIA grade of spine cord injury according to its effective intension (P < 0.05).

CONCLUSIONThe main prognostic factors on non-operative treatment of cervical spinal cord injury without fracture or dislocation were MRI type and extent of spine cord injury, meanwhile, correlate with effective cervical spinal canal ratio, Pfirrmann grade of intervertebral disc herniation, ASIA grade of spine cord injury. It is deliberative to choose no-operative treatment, for it only refers to the patients with the mild localized edema type or no signal change of spinal cord in MRII. Operative treatment shoud be recommended for other patients.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Cervical Cord ; diagnostic imaging ; injuries ; surgery ; Female ; Humans ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Prognosis ; Radiography ; Retrospective Studies ; Spinal Cord Injuries ; diagnosis ; diagnostic imaging ; surgery ; Young Adult

Objective To investigate the role of tracheotomy in children with severe hand foot and mouth disease.Methods Eight children with severe hand foot and mouth disease collected from July 2009 to August 2011 in PICU were retrospectively reviewed.Clinical manifestation and laboratory data were analyzed and followed up.Results Eight patients were enterovirus 71 (EV71) positive.Hormones,intravenous immune globulin,mechanical ventilation and vasodilators had been administered in patients with cardiopulmonary failure.They had severe neurologic sequelae,requiring prolonged intubation.MRI imaging of 7 cases showed abnormal brainstem,pons and medulla oblongata,1 case showed swelling of brain tissue.A total of 8 patients underwent a tracheotomy in mean (25.63 ± 8.86) d.The older was 22 months,the younger was 8 months and the mean age was (13.13 ±4.22) months.Four cases died,the other survived.Two cases were successfully decannulation,the other two were still in the tracheotomy.Conclusion Tracheostomy play an active role in reducing airway resistance,clearing airway secretions,providing the opportunity for subsequent rehabilitation.Eight patients were followed up for three years,neurological sequelae could not be restored.Four cases repeatedly suffer from pneumonia,atelectasis,leading to a serious decline in quality of life.

BACKGROUND:Knee cartilage injury is difficult to heal, which is an urgent clinical problem to solve. OBJECTIVE:To summarize the advantages of synovial mesenchymal stem cel s in the treatment of knee cartilage injury. METHODS:A computer-based search of PubMed was performed for articles related to synovial mesenchymal stem cel s for knee cartilage injury using the keywords of“knee joint, cartilage, synovial membrane, tissues, injuries and repair”. A total of 48 articles were retrieved initial y, and 35 articles were included in result analysis. RESULTS AND CONCLUSION:Synovial mesenchymal stem cel s for treatment of knee cartilage injury can achieve more ideal outcomes. Mesenchymal stem cel s inherent in the damaged tissue are the optimal seed cel s for tissue repair.

AIM:To investigate the relationship of autophagy and reactive oxygen species ( ROS) in multiple myeloma cell line RPMI-8226 stimulated with doxorubicin.METHODS:The RPMI-8226 cells were stimulated with doxo-rubicin at different doses, and untreated cells were used as control.The protein expression of beclin 1 and LC3 was detec-ted by Western blot.ROS production was analyzed by DCFH-DA fluorescence staining.After treated with or without 3-methyladenine (3-MA), the ROS production and apoptosis in RPMI-8226 cells were determined by DCFH-DA and flow cy-tometry, respectively.After treated with or without antioxidants tempol and N-acetyl-L-cysteine ( NAC) , the expression of beclin 1 and LC3 in RPMI-8226 cells was determined by Western blot.RESULTS:The protein levels of beclin 1 and LC3Ⅱ/LC3Ⅰwere increased in the RPMI-8226 cells stimulated with doxorubicin compared with untreated group.The ROS production was increased in the RPMI-8226 cells stimulated with 2 mg/L doxorubicin compared with untreated group.After treated with 3-MA, the ROS production and apoptosis in the RPMI-8226 cells stimulated with doxorubicin were increased compared with doxorubicin group.After treated with antioxidant NAC or tempol, the expression of beclin 1 and LC3 II/I in the RPMI-8226 cells stimulated with doxorubicin was decreased compared with doxorubicin group.CONCLUSION:The autophagy and ROS levels are increased in RPMI-8226 cells stimulated with doxorubicin.Inhibition of autophagy increases the ROS production and apoptosis of RPMI-8226 cells stimulated with doxorubicin.Inhibition of ROS production reduces doxorubicin-induced autophagy in multiple myeloma cells.