The factors influencing public access to life science information were analyzed from its scope, cost, chan-nels, mechanisms and laws with suggestions put forward for the access to life science information without any barrier.

Objective To investigate the antithrombotic effects of polydatin.Methods Injection of arachidonic acid into mouse,electrically stimulated carotid artery and inferior vein ligation in rats were used to evaluate polydatins antithrombotic effects respectively.Results Polydatin presented obvious antithrombotic effects in three thrombotic models and showed a good dose-effect relationship.Conclusion It is suggested that polydatin has evident antithrombotic effects in artery,vein and micro-circulation.

Chronic myeloid leukemia (CML) is a malignant clonal proliferative disease,which originates from multifunctional hematopoietic stem cells of bone marrow.Imatinib is used as the first-line treatment for CML currently,owing to its obvious curative effect for patients in chronic phase and survival time prolonged significantly for patients in accelerated phase and blast crisis.With the long-term application of imatinib in clinic,the drug resistance and intolerance gradually appear,resulting in decreased long-term effects of CML.This paper will review the current status of the pathogenesis and treatment of CML.

Medical clinical practice plays an important role in medical education, which influences the quality of medical peoples. In clinical practice teaching of respiratory medicine, many factors have a major impact on the quality of training. This article focuses on chnical practice teaching in influence factors and puts forward some countermeasures.

ObjectiveTo discuss clinical application value of contrast-enhanced ultrasound(CEUS) technology in the diagnosis of liver and spleen trauma. MethodsRetrospective analysis 25 cases of liver and spleen trauma patients, the conventional ultrasonic CEUS complete material, analysis of the liver and spleen damage CEUS performance,and with surgery and computed tomography(CT) strengthen comparative analysis of the sensitivity of the conventional ultrasonic, CEUS, specific degree and the classification accuracy. ResultsAmong the surgery or CT confirmed 23 cases for liver and spleen rupture,the pure 8 cases liver, injury 13 cases of pure spleen rupture ,liver and spleen composite injury in 2 cases. And surgery or CT comparison, the sensitivity of the CEUS and specific degrees were 90.5％ and 66.7％ ,significantly higher than conventional ultrasound 59. 1％ and 33.3％ (P ＜0.05) ;CEUS classification was 91.3％ (21/23), significantly higher than the rules of ultrasound was 65.2％ ( 15/23 ), both differences were statistically significant( P ＜ 0.05). ConclusionCEUS diagnosis liver and spleen, due to the sensitivity of the damage degree high, and the classification accuracy was higher, the diagnosis to accurately evaluate the extent of the damage to the liver and spleen in liver and spleen, the loss of the diagnosis and treatment of clinical guidelines choice had important value of clinical application.

The possible relationship of vesicular glutamate transporters ( VGluT1 and VGluT2 ) - and 5-HT-like immunoreactive (LI) terminals with mesencephalic trigeminal nucleus (Vme) neurons in the rat was examined by using triple-immunofluorescence histochemistry and double-labeled electron microscopic immunohistochemistry. Under confocal laser-scanning microscope, many neuronal cell bodies of Vme showed phosphate-activated glutaminase (PAG) -LI and the vast majority of them were large pseudounipolar neurons. A considerable number of VGluT1 -LI and VGluT2-LI terminals were widely distributed in Vme, the density of VGluT2-LI terminals was higher than that of VGluT1-LI. 5-HT-LI axonal varicosities had dense distribution in Vme, and some 5-HT-LI terminals also showed immunoreactivity for VGluT2. Some VGluT1-LI, VGluT2-LI, 5-HT-LI and VGluT2/5-HT-LI terminals were frequently observed in close apposition to the cell bodies of Vme neurons showing PAG-LI. Under electron microscope, VGluT1/VGluT2-LI and 5-HT-LI were visualized with silver grains and peroxidase products, respectively. Some terminals in Vme showed both VGluT2- and 5-HT-LI, these dual labeled varicosities usually made asymmetric contact with Vme neurons. Synaptic terminals that showed VGluT1-LI was also observed, but no coexpression of VGluT1 and 5-HT was found in Vme. The present results suggest that in the transmission of the proprioceptive sensory information from the orofacial regions to the higher center, glutamate and 5-HT may play important roles on the regulation of Vme neurons through complicated integration.

Objective To investigate the cause,prevention and treatment of bile leakage. MethodsThe clinical data of 22 cases with bile leakage treated from Jan.1993 to Dec.1998 were reviewed retrospectively. Results Of the 19 cases treated with nonoperative therapy,1 patient died,3 patients were transferred to be operated later, and the other 15 cases were cured. The cure rate of nonoperation was 79%(15/19). 3 patients were cured with emergent operation at the beginning of bile leakage. Of the 3 cases who were transferred to be operated later,2 cases were finally cured by operation while the other 1 patient was not cured.The total cure rate was 91%(20/22). ConclusionBile leakage often oocurs in cholecystectomy procedure and after removal of a T tube, which is mainly related to inflammation, adhesion, abnormality of anatomy and incorrect manipulation. In order to prevent it,surgeons should pay more attention to the 3 links of prirnary procedure, including preoperative preparation,operative management and postoperative treatment. Different treatments are optional according to the degree of leakage and the condition of patients.

ObjectiveTo explore the etiology of incision hernia of abdominal wall. Methods 72 cases with incision hernia were analyzed retrospectively in types of incision, technique of suture, materials, infection of incision, increase of intraabdominal pressure, age, nutrition and time of occurrence. ResultsLongitudinal incision, bad technique of suture,infection of incision, increase of intraabdominal pressure, aged, hypoalbuminemia and diabetes mellitus easily induced incision of hernia. ConclusionTransverse incision should be adopted if operation and safety are not affected. Prevent and treat infection or rupture of incision by strict aseptic manipulation, hemostasis and right use of antibiotics. Prevent increase of intraabdominal pressure in every aspect. Pay atention to nutrition supply of the elderly and promote healing of incision. Postoperative half year is high incidence stage of incision hernia and all inducing factors should be avoided.

Objective To test the reliability and validity of the Chinese version Diabetea Self-Care Behavior(SDSCA)Questionnaire,and thus to provide an effective and convenient way of self-care behavior assessment for patients with type 2 diabetes.Methods The questionnaire based on the translation of SDSCA wag used in 20 cases of type 2 diabetes patients,in order to analyze the retest rehability,internal consistency and scale construct validity. Results The retest reliability of each dimension wag between 0.763 to 1.000;the scale correlation coeflicients between items in specific diet dimension Wag lowest(0.104),and the others were moderate to high degree of correlation between 0.555～0.933;exploratory factor analysis extracted 4 components,and the percentage of variance explained was 67.483% .Conclusions Chinese version SDSCA has a good reliability and validity,and is good for evaluating the self-care activities in type 2 diabetes patients.

Objective To observe the association of vesicular glutamate transporter of typeⅠ (VGluT1 ) like immunoreactive(LI) ,the differentiation-associated Na+ dependent inorganic phosphate cotransporter(DNPI) LI and glutami cacid decarboxy lase(GAD) LI terminals with GABAA receptor ?3 subunit(GABAAR ?3) LI neurons in mesencephalic trige minal nucleus of the rat.Methods Triple immun of luorescencehis to chemical staining technique and confo call aser scanning micros copy were used.Results Alargenumber of neuronal cell bodies showed GABAA R?3 LI immunoreactivity atallrostrocaudal level softhe Vme ,and most of GABAAR ?3 LI cells were large (2 5 5 0 ?m)pseudouni polarneurons.The dense VGluT1 LI,DNPL LI and GAD LIterminal sdistri buted widelyin Vme ,some VGlu T1 DNPI LI and GAD LIterminals surrounded the somata of the GABAAR ?3 LI Vmeneurons ,and made close contacts with them .Conclusion Proprioceptive sensory signals from the or of acialregionmight be modulated at the level of the primary afferent cell bodies in the Vme both by glutamatergic and GABAergic axonal terminals from other brain areas,and the effect of GABAergic terminals might be mediated by post synaptical GABAA receptors .