0.05)and the treatment costs were 1 104.60 yuan,2 049.24 yuan and 3 247.99 yuan,respectively(P

Parkinson’s disease is resulted from the progressive degeneration and from the loss of dopaminergic neurons in Substantia Nigra. Current researches focuson the optimum conditions in vivo and in vitro associating the differentiation as well as survival of dopaminergic neurons,in order to get sufficient dopaminergic neurons for cell-replacement therapy in Parkinson’s disease. This paper reviewed influential factors of neural stem cells differentiate into dopaminergic neurons in vivo and in vitro.

Objective To observe biological character iistcs of cultivated cornea limbal epithelial cells of rabbit.Methods Cultivated cornea limbal epithelial cells with tissue inoculation were observed by inverted microscope every two days; ultrastructure of cell was observated by electron microscope; Proliferative ability of cell was examined by flow cytometry; the cell growth curve was examined by monotetrazolium(MTT) colorimetry.Results Cultivated limbal cornea epithelial cells formed monolayer at six day,the cultivated conea limbal epithelial cells had high metabolism and proliferative ability, growth of cornea limbal epithelial cells reached climax after 4 days by passage culture. Conclusion Cultivated limbal epithelial cells during the first and second growth generation can keep cornea epitheliam property and high proliferative ability.

Objective To investigate the clinical and imaging features of the reversible posterior leukoencephalopathy syndrome(RPLS).Methods The clinical and imagig data of 9 patients with RPLS were analyzed retrospectively.Results Among the patients,4(44%)were secondary to pregnancy-induced hypertension syndrome,1(11%)was second to short bowel syndrome,1(11% )was second to acute lymphoblastic leukemia,1( 11% )was second to IgA nephropathy,and 2(22%)were second to hypertensive encephalopathy.Their mean age of symptom onset was 23.4 ± 9.9 years.Their main clinical manifestations were headaches,seizures,blurred vision,and consciousness disturbance.Magnetic resonance imaging(MRI)showed symmetrical lesions of bilateral white matter in posterior brain.T1 weighted imaging and diffusion-weighted imaging showed low or isointense signal.T2 weighted imaging,fluid attenuated inversion recovery(FLAIR),and apparent diffusion coefficient(ADC)map showed high-intensity signal.The symptoms of the patients disappeared after the treatment.MRI reexamination showed that the lesions disappeared completely or almost completely.Conclusions The causes of RPLS were different.The imaging mainly showed the signal of vasogenic brain edema on the posterior white matter of the brain.After timely treatment,the symptorms and imaging features were improved rapidly.The patients with young-onset RPLS are their important clinical feature.[Key wonds]Posterior leukoencephalopathy syndrome;Magnetic resonance imaaging;Hypertension;Age factors were improved rapidly.The patients with young-onset RPLS are their important clinical feature.

Objective To investigate the effect of acute or chronic administration of eseitalopram(ESC)on behavior of mice,which suffered from chronic unpredictable mild stress(CUMS).Methods Mice were exposured to CUMS for 8 weeks,then the behavior of mice were investigated every 2 weeks,and the effect of acute and chronic administration of escitalopram on behavior was compared.Results (1)The body weight of all stressed mice were lighter than control group(18.5±1.1)g from 2nd to 8th week(P＜0.01).After chronic administration of escitalopram,the body weight of cms+Cesc group(18.5±0.6)g were increased relative to other stressed groups(P＜0.01).(2)In open-field test for 15min,the total distance and central distance of stressed groups were further,and numbers of rear were more than control group(P＜0.05)from 2nd to 4th week,but then these indexes were decreased from 6th to 8th week(P＜0.05).After chronic administration,the total distance(57.4±11.0)m,central distance(12.0±3.0)m and numbers of rear(74.2±6.1)of cms+Cesc group increased(P＜0.05).(3)In sucrose preference test,the preference percentage of stressed groups was less than control group(P＜0.05)from 6th to 8th week at 8th week,the preference percentage of cms + Cesc group((79.0±2.7)%)was more than other stressed groups(P＜0.05).(4)In forced swimming test,compared with control group,immobility time of stressed groups became longer from 4th to 8th week..After chronic administration,immobility time of cms + Cesc group((124.7±8.00)s)was shorter than other stressed groups.(5)After acute administration,the behavior of cms + Aesc group was not different relative to pure stressed group(P＞0.05).Conclusion Chronic administration of escitalopram show up efficacy on depression behavior of CUMS mice,but acute administration is ineffective.

Objective:With the development of science and technology, large medical equipments have been universally applied in clinical diagnosis. It is important in hospital management to search for the way to obtain the maximum economic and social benefits by efficiently analyzing cost benefit of large medical equipment. Methods:To ascertain cost benefit of medical equipment by profit margin of investments and cost-redemption periods. Results:Proposals on purchase and management of department medical equipment were presented. Conclusion:Scientific evaluation and analysis on large medical equipment would play a positive role in purchase, use and economic returns of department medical equipment.

Objective To establish a management information system for bacteria ( virus ) preservation in order to improve the management efficiency and quality of bacteria ( virus) preservation organizations to the largest possible extent . Methods Client/Server(C/S) structure was adopted to design the system with Visual Basic 6.0 and SQL Server2000 as the platform of development .A bacteria ( virus) information database was established with the E-R model and 3NF.The system was developed by face to object language program .Results Devices needed were supplied .Functional modules of bacteria ( virus ) information management , scientific research project management , user management and system maintenance were constructed .Visiting permission was installed on the platform and database to maximize the protection of bacteria ( virus ) information safety .Conclusion Bacteria ( virus ) information flow management , involving storage , classification, preservation, collection and record, is achieved.All the resources of the preservation organization are integrated.Bacteria (virus) management efficiency is increased and bacteria (virus) information safety is ensured.

OBJECTIVE To cut down the occurring rate of the hospital infections in the neonate ward. METHODS To summarize the experiences and to approach the methods for the infection control and the nursing management for hospital infections in neonate ward in last ten years(1995-2004). RESULTS Thermostats,blue-ray boxes,resuscitators,respirator tubes and some other devices which usually applying in neonate ward were easily lead to the hospital infections. CONCLUSIONS The nursing department plays an important role in controlling the hospital infections,and the head nurse is the key to the question,and all the members mobilization is the guarantee for putting the measures into effect.

Background: The quality of life has been greatly influenced and the cost of medical expenses is very high in patients with irritable bowel syndrome (IBS). The etiology and pathogenesis of IBS are still unclear, and the prevention and treatment of this disease still lack of effective methods. Objective: To explore and analyze the effects of Tiaohe Ganpi Hexin Decoction (TGHD), a compound traditional Chinese herbal medicine for regulating the liver and spleen, on IBS patients with diarrhea. Design, setting, participants and interventions: All 40 IBS patients came from the First Hospital of Jinan University, Guangzhou Red Cross Hospital, and the First Affiliated Hospital of Guangzhou University of Chinese Medicine, and were randomly divided into two groups. Patients in the treatment group (n=20) were given TGHD, while those in the control group (n=20) were prescribed oral pinaverium with a four-week treatment period. Main outcome measures: Traditional Chinese medicine (TCM) syndrome score, total obviously effective rate, disappearance rate of symptoms, and clinical symptom score in the two groups were evaluated before and after four-week treatment. Results: After the treatment, TCM syndrome scores in both groups were decreased (P<0.01), and the TCM syndrome score in the treatment group was significantly lower than that in the control group (P<0.01). There was a significant difference in the total obviously effective rate between the two groups (P<0.01), and the total obviously effective rates in the treatment and control groups were 85%(17/20) and 45%(9/20) respectively. The disappearance rates of abdominal pain, abdominal distention, poor stool output, stool frequency, stool character and mucous stool in the treatment group were higher than those in the control group (P<0.05). The symptom scores of abdominal pain, abdominal distention, poor stool output, stool frequency, stool character and mucous stool in the treatment group were lower than those in the control group (P<0.05). Conclusion: TGHD can significantly improve the clinical symptoms in IBS patients with diarrhea.

Objective Resveratrol can improve nonalcoholic fatty liver disease , but its action mechanisms remain unclear . This study aimed to investigate the protective effect of resveratrol against the free fatty acid ( FFA)-induced apoptosis of human hepatic L02 cells and its possible mechanisms . Methods Human hepatic L02 cells were incubated with FFA and resveratrol for 24 hours.The prepared cells were divided into a blank control , an FFA ( 2 mmol/L) , and a resveratrol group ( 50 μmol/L resveratrol +2 mmol L/FFA).After treatment, we measured the triglyceride (TG), glutathi-one (GSH), and malonaldchyde (MDA) contents and caspase3 ac-tivity in the hepatocytes , determined the apoptosis of the cells by flow cytometry , and detected the protein expression of silent information regulator 1 (SIRT1) by Western blot as well as the mRNA expressions of catalase (CAT), Mn superoxide dismucase (MnSOD), Bcl-2, and Bax by qRT-PCR. Results The TG content and caspase 3 activity in the hepatocytes were significantly increased in the FFA ([3518.±64.2] μmol/L and [5.97 ±0.78] U/g) and the resveratrol group ([201.1 ±60.1] μmol/L and [3.60 ±0.73] U/g) as compared with those of the blank control ([40.2 ±7.4] μmol/L and [2.56 ±0.49] U/g) (both P<0.05), but the caspase3 ac-tivity was markedly decreased in the resveratrol group in comparison with that of the FFA group (P<0.05).Both early and late apop-tosis rates of the hepatocytes were remarkably higher in the FFA ([6.75 ±0.81]%and [8.52 ±0.54]%) and the resveratrol group ([4.94 ±0.44]%and [6.52 ±0.61]%) than those in the blank control ([3.38 ±0.33]% and [2.72 ±0.19]%) ( both P<0.05), with statistically significant differences between the former two groups (P<0.05).The resveratrol group showed significant differences in the GSH content ([100.2 ±8.8] nmol/g), the MDA level ([2.36 ±0.82] mg/g), and the relative expression of SIRT1 (0.84 ±0.04) from the FFA group ([73.8 ±13.1] nmol/g, [3.77 ±0.92] mg/g, and 0.61 ±0.07) and the control ([113.7 ±13.8] nmol/g, [1.85 ±0.41] mg/g, and 0.90 ±0.02) (all P<0.05).The resveratrol group also exhibited statistically significant differences in the relative expressions of the MnSOD , CAT, and Bax genes from the FFA and control groups (P<0.05). Conclusion Resveratrol attenuates FFA-induced apoptosis of human hepatic L 02 cells by activating SIRT1 and reducing the oxidative stress of hepatocytes .