The external genital organs were examined in 1822 male military recruiters from 2004 to 2008 in Beijing.The prevalence of redundant prepuce was 67.07%.that of phimosis was 2.36%and that of moderate to severe varicocele Writs 1.43%.Among all examinees 68(3.73%)recruiters failed physical examination due to phimosis or moderate to severe vailcocelel.We suggest a joint effort should be made by community health centers and urological specialists to check up genital organs of male youngsters,and health education should be provided in schools,so early cure would be possible and qualification rate of recruiters could be raised.

BRCA1-associated RING domain 1(BARD1) is composed with ring finger,ankyrin repeats and BRCT (BRCA1 C-terminus) domain.The special structure is involved in many essential biological processes such as DNA repair,cell survival and apoptosis,proteasome dependent protein degradation and so on.The abnormal expression of BARD1 is closely related with the occurrence and progression of carcinoma.Aberrant splice variants of BARD1 are very potential tumor markers for cancer diagnosis.

Objective To explore the clinical effect of atorvastatin on acute cerebral infarction treated with atorvastatin in the elderly.Methods 136 patients with acute cerebral infarction treated in our department from Oct.2011 to Nov.2012.And the patients were divided into observation group and control group.In observation group,68 cases were treated with conventional therapy plus Atorvastatin combined with aspirin.In control group,68 cases were treated with conventional therapy and aspirin.The clinical effect and safety were observed.Results In observation group versus control group,the cure rate (42.7％ vs.29.4％,x2 =2.584,P =0.108) and total effective rate (86.8％ vs.79.4％,x2 =1.821,P=0.177) showed no statistically significant differences.Posttreatment versus pre-treatment showed that neurological deficit score was improved both in observation group(t=18.621,P＜0.001) and in control group(t=15.026,P＜0.001) and activities of daily living improved both in observation group (t=10.544,P＜0.001)and in control group (t=8.815,P＜0.001).The differences of in neurological deficit score (t=1.179,P=0.120) and activities of daily living (t =0.321,P =0.374) were not statistically significance between the two groups.Before treatment,there were no significant differences in total cholesterol (TC),triglyceride (TG),low density-lipoprotein-cholesterol (LDL-C) and high-density-lipoprotein-cholesterol (HDL-C)between the observation group and the control group.After treatment,the difference in improvement of TC (t=8.446,P＜0.001),TG (t=4.145,P＜0.001),LDL-C (t=3.439,P=0.001) and HDL-C(t=2.718,P=0.004) between observation group and control group were statistically significant.Conclusions Atorvastatin combined with aspirin as add-on therapy to conventional therapy plus aspirin has better efficacy in the treatment of acute cerebral infarction in the elderly,and improve the blood lipid related indicators and life activity.

According to management status of apparatus in Lianyungang CDC applied for national laboratory approval and the claim of ISO/IEC 17025:2005,the application of Lianyungang CDC laboratory apparatus is investigated for improving the mode of management in future.

Following high velocity projectile wound to dog legs, some indexes reflecting hepatic functions were measured. It was found that at 24 hr after wounding the ATP and phospholipid levels of the liver decreased, and that the OCT activity in serum and liver MDA content increased. The results revealed that the hepatic function impaired at 24hr after injury. It suggested that the hepatic function should be monitored and maintained even in the early period of high velocity projectile injury.

Objective To investigate the common critical value (CV) of patients hospitalized in the department of spine surgery and to determine the corresponding management. Methods Medical records of patients between 2013 January and 2014 December were retrospectively reviewed. 47 cases with CV were noted. The following characteristics of CV were recorded and analyzed, including time-point at occurrence, type, reason and management. Results Most of the CVs (43/47, 91.5%) were detected between 8 am to 12 am, and between 17 pm and 21 pm. Overall, there were 24 cases of abnormal blood electrolyte (51.1%), 13 cases of abnormal routine blood test (27.7%), 5 cases of abnormal blood coagulation test (10.6%), and 1 case of abnormal TnT (2.1%). The remaining 4 cases including 2 cases detected in the night (1 case of abnormal blood gas test and 1 case of abnormal TnT) and 2 cases detected in the afternoon (1 case of positive blood bacteria culture and 1 case of low blood glucose level). Conclusions The most common CVs were summarized in the clinical practice. The findings have important clinical implications concerning the management of these CVs. In addition, preventive modality of daily nursing and standardization of medical management should be applied so as to ensure the safety of patients and rapid recovery.

In order to improve the optimum temperature of lipases, The Penicillum expansum lipase (PEL) gene was mutated by site-directed mutagenesis using overlap extension PCR technique. The recombinant plasmid pPIC3.5K-lip-E83V containing matant gene was expressed in Pichia pastoris GS115. The comparison experiments of the mutant PEL-E83V-GS with the wild-type PEL-GS showed that: the optimum temperature of the mutant (45℃) was higher by 5℃ than that of the wild type. The thermostability of the mutant was similar to the wild type. The enzymatic activity of the mutant was 188 U/mL at 37 ℃, which was 80% that of the wild type at the same conditions. The hydrophobic interaction may be enhanced in the surface region by the hydrophilic amino acid, Glu substituted with the hydrophobic amino acid, Val, and be responsible for the improvement of optimum temperature.

Objective To assess the application value of serum procalcitonin(PCT)for the differentiation between the neonates with or without septicemia.Methods PCT,C-reactive protein(CRP),blood and blood culture tests were done in 75 cases of neonatal septicemia as the therapy group,and 74 neonates without the signs of infection as the control group after the admission of our hospital One week later,the therapy group also re-examined the PTC and CRP tests.Furthermore,the results were analyzed.Results The levels of CRP(23.26±5.68)mg/L and PCT(7.61±4.53)ng/L in therapy group were dramatically reduced compared with(4.09±1.09)mg/L,(1.95±0.25)ng/L in therapy group,and(6.42±4.54)mg/L,(0.45±0.16)ng/L in the control group before therapy,the t values are 28.71,12.97 and 19.97,14.08 respectively,and p value are both less than 0.01.The positive rates of PTC,CRP and blood culture were 82.7％,48.0％ 28.0％ before the admition to hospital.The differences of PTC and CRP,PTC and blood culture had statistical significances(x2=5.64,P＜0.05;x2=10.40,P＜0.01).If 2.5 μg/L of PCT and 8mg/L of CRP would be regarded as cutoff point in our study,the sensitivity and specificity of PCT or CRP for diagnosis of neonatal septicemia was 82.7％ and 80.3％,48.7％ and 78.4％.Conclusion Our data suggested that the serum PTC is a valuable marker for the early diagnosis of neonatal septicemia.

Objective: To provide policy recommendations for improving price negotiation system of patented drugs in China. Methods: This paper comparatively analyzes the commonalities and characteristics between Korea’ and Germany’s price negotiation models for patented drugs from three aspects of their goals, procedures and effects. Results:The key objective of both Korea’ and Germany’s price negotiation systems for patented drugs is to efficiently improve the utilization of national health insurance services, and responsibilities are properly assigned among differ-ent institutions to ensure the equity and efficiency of negotiations. However, due to the differences in national cir-cumstances, there is a big difference in the selection of technical assessment criteria. Conclusion: This paper sug-gests China to strengthen the convergence between price negotiations for patented drugs and medical insurance reim-bursement policy, to establish a technical assessment system with the innovation extent for patented drugs as its core, and to develop scientific and rational negotiation procedures and division of responsibilities as well.

Objective To study the effect of prevention and treatment for patients with radiation proctitis by retention enema.Methods According to the digital table,120 patients with pelvic tumor treated with radiotherapy were randomly divided into two groups.The observation group was taken retention enema 2 to 14 days after radiotherapy once a day.The control group was taken retention enema when the patients with radiation proctitis after radiotherapy.The severity and frequency of radiation proctitis were observed in the two groups.Results The incidence of radiation proctitis in the observation group was 35.0％,which was significantly lower than that of the control group (83.3％) (χ2 =47.623,P =0.000).The severity of radiation proctitis was also significantly lower than that of control group.The incidence of Ⅱ and Ⅲ degree of radiation proctitis in the observation group were 3.3％,0,respectively,which were significantly lower than those of the control group (26.7％,6.7 ％) (χ2 =12.810、4.138,P =0.000、0.042).The total efficiency rate was 100.0％,which was significantly higher than that of the control group (72.0％)(χ2 =32.558,P =0.000).Conclusion Retention enema can effectively treat the radiation proctitis and the effect will be more significant if the retention enema starts soon after radiotherapy.