MicroRNA (miRNA) regulate the expression of target mRNA according to the specific base pairing. They are involved in proliferation,differentiation and apoptosis of cells. The expression of miRNA has distinct differences between normal tissues and cancers, miRNA can be oncogenes but also suppressor genes, they play an important role in cancer. And miRNA are promising to be a new therapeutic target. In this review, we will discuss the advance in the research of miRNA in cancer.

BACKGROUND: Cecropins are a kind of micromolecule protein with antibacterial activity. Eukaryotic cell-expressed or artificially synthesized Cecropins is characterized by low efficiency and high cost. OBJECTIVE: To clone and express an antibacterial peptide gene of Musca domestica Cecropin A, and to identify recombinant expression product. METHODS: Mature Musca domestica Cecropin A encoding nucleotide sequence was searched from the GenBank and amplified by RT-PCR. The gene of Musca domestica Cecropin A was cloned into prokaryotic expression vector pET32a and fused with gene of Thioredoxin (Trx) and expressed in E.coli BL2l (DE3). After induction by isopropyl-β-D-thiogalactoside, the sera of the immunized rabbits were collected after rabbits were immunized with the hemolymph of housefly larvae. Recombinant protein was identified by western blot analysis and N-[Tris(hydroxymethyl)methyl]glycine-sodium dodecylsulfate-polyacrylamide gel electrophoresis.RESULTS AND CONCLUSION: After induction by isopropyl-β-D-thiogalactoside, E.coli BL21 expressed mature Cecropin. Rabbit anti- housefly larvae sera, N-[Tris(hydroxymethyl)methyl]glycine-sodium dodecylsulfate-polyacrylamide gel electrophoresis and western blot analysis results confirmed that expression products were mature Cecropin. These suggest that prokaryotic expression system can be utilized to obtain natural mature Cecropin.

Objective: To observe the clinical effects of needling acupoints Shenting(GV 24),Neiguan(PC 6) and Sanyinjiao(SP 6) in the treatment of migraine. Methods: Thirty cases of Germen patients with migraine were treated mainly by Shenting(GV 24), bilateral Neiguan(PC 6) and Sanyinjiao(SP 6), as well as bilateral Hegu(LI 4) and Taichong(LR 3), Touwei(ST 8),Yangbai(GB 14), Cuanzhu(BL 2), Sizhukong(TE 23), Shuaigu(GB 8) and Sibai(ST 2) in the affected side of head, auricular Sympathetic (MA-AH 7), Er Shenmen (MA-TF 1), Heart (MA-IC) and Endocrine (MA-IC 3); another 28 cases were treated by routine acupuncture. The clinical results and immediate pain-relieving effects were observed. Results: The total effective rate was 90.0% in treatment group and 75.0% in control group, χ2= 4.57, P ＜ 0.05; the 24-hour pain-relieving effects were obviously better in treatment group than that in control group, χ2 = 10.11, P＜ 0.01. Conclusion: Needling Shenting(GV 24), Neiguan (PC 6) and Sanyinjiao(SP 6) has better effects than routine acupuncture in the treatment of migraine.

Atrial fibrillation is the most common arrhythmia in clinical practice.However,the therapeutic effects of traditional antiarrhythmic medicines are far from satisfactory,because of the high rate of arrhythmia recurrence and the potential proarrhythmia effect.Recently,with the further investigation of the mechanisms that initiate and perpetuate atrial fibrillation,and the support of extensive data from clinical trials and animal experiments,thera-peutics based on anti-inflammation,antioxidation,regulation on renin-angiotensin-aldosterone system(RAAS)and gap junction may be new therapeutic targets for the treatment of arrhythmia.In this paper,the potential molecular mechanisms involved in these processes and several clinical trials about some non-traditional antiarrhythmic medi-cines were reviewed,in order to propose a new idea about the development of antiarrhythmic medicines.

Aim To investigate the apoptosis of cervical cancer HeLa cells cultured in vitro transfectead by combining telomerase anti-hTR and anti-hTERT. Methods The activity of telomerase was tested by polymerase chain reaction-telomeric repeat amplification protocolenzyme-linked immunosorbent assay (PCR- TRAP- ELISA). Cell morphologies were observed by fluorescence microscope stained with acridine orange. Apoptosis and cell cycle were examined by Flow Cytometer method (FCM). Results The telomerase activity of HeLa cells transfected by antisense oligonucleotides were significantly decreased compared with those of nontreated control,sense oligonucleotides and oligofectamine TM(P

Objective: T o further investigate pathogenesis about vulvovaginal candi diasis (VVC). Methods: Human normal vaginal epithelial cells in vitro were cultured by tissue cultur e and keratinocyto serum-free medium. Passaging epithelial cells were coculture d with candida albicans medium in separate wells for 0, 3, 6, 12, 24, and 48 h. C ontr ol epithelial cells were cultured alone in separate wells. For examination o f cytokines and chemokines, at each time point, the coculture supernatant and th e control culture were collected. ELISA was applied to detect the levels of HBD -1, HBD-2 and SP-A protein expression in the vaginal epithelial cells (HBD-1 :F= 62.784,P=0.001; HBD-2: F=5127.984, P=0.000; F=542.210, P=0 .000). Also, we colle cted the vaginal doughing water of VVC patients and healthy women, and detected the levels of SP-A, then analysed the data. Results: In the tra il of human vagin al epithelial cells, in contrast to the control group, the medium group induced the epithelial cells to secrete more HBD-1, HBD-2 and SP-A at h48, which was co nsidered to be significant (P

Epidemiological and clinical studies have provided compelling evidence that suggests a link between the relative lack of infectious diseases and the increase in allergic disorders. Asthma usually starts early in life. The early occurrence of asthma suggests that prinatal influences are of importance.Prenatal or postnatal exposure to endotoxins and other bacterial products may protect against the development of asthma in the infant. We will briefly outline the current state of the relationship of the perinatal infection and asthma in the infant, and focus our review mainly on possible rneehanism of perinatal infection providing protection against asthma in the infant, such as Th1/Th2 immune system, transcription factors, protection by maternal antibodies, endotoxin tolerance, pulmonary inflammation and genetic influences.

Cell Research got all impact factor of 3. 426, which was the highest Chinese SCI journal, but ranked 4th by journal's h index. By analyzing the cites and citations of Cell Research from 1999-2006 using the method of bibliometrics, four main characteristics of Cell Research were found:①The quantity of papers published Oil Cell Research is low and their quality is hish. ②Advantageous opening, with authorship patterns, editorial members and peer reviewers from home and abroad. ③Journal published in English is easy to perform international academic exchange. ④International operation model also played an important role in Cell Research's success.

Causality ; Humans ; Occupational Exposure ; Vibration ; adverse effects

Severe acute pancreatitis is a relatively common surgical disease. Patients have a hypermeta-bolic state and nutritional support for patients with SAP is needed. Early enteral nutrition support is a viable option and can improve the nutritional status of patients, protect the gut barrier function, reduce bacteria translocation to regulate the inflammatory response and reduce hospitalization costs. Combined with our do-mestic and foreign literatures in recent years, this review the feasibility and practical implementation of enter-al nutrition in severe acute pancreatitis treatment summarizs.