Objective To investigate the effct of PTTG gene on the growth and sensitivity to 5-FU of cholangiocarcinoma cell.Methods The pcDNA3.1-PTTGas that contained full-length antisense PTTG,and pcDNA3.1(+) was transfected into the cholangiocarcinoma cell line QBC939.We successfully established and identified cell sublines tQBC939(PTTG-),tQBC939(pcDNA3.1).Cell growth curve was mapped out,cell proliferation rate was assessed by MTT assay and cell cycle percentage was counted by flow cytometry.After treatment with 5-FU,cell survival rate was assessed by MTT assay and IC50 was calculated.Flow cytometry and Hoechst staining were used to demonstrate discrepancy of cell apoptosis rates.Results Compared with other two groups,tQBC939(PTTG-) grew more rapidly and had a higher proportion of cells in S phase,whereas less cells in G2/M phase(P

OBJECTIVE: To investigate the effects of purslane herb aquenous extracts (PHAS) on the stress ability of aging mice induced by D-galactose. METHODS: We observed the survival time to hypoxia and heat survival rate of the mice treated with different doses of PHAS and vitamin E. The contents of lipofuscin and malondialdehyde (MDA), and the activity of superoxide dismutase (SOD) and catalase (CAT) in the brain and liver of the mice were tested. RESULTS: As compared with vitamin E, three doses of PHAS (1.6, 0.8 and 0.4 ml/d) prolonged the survival time to hypoxia and the pole climbing time and increased the heat survival rate, and the 0.8 ml/d PHAS had the best effect. In the group of 0.8 ml/d PHAS, the activity of SOD and CAT decreased less, and the contents of lipofuscin and MDA decreased significantly. The effect of vitamin E was not as good as the PHAS. CONCLUSION: PHAS can prevent the stress ability of the aging mice. One of its mechanisms may be increasing the activity of SOD and CAT, hence decreasing the damage of the oxidation products to the body.

Objective To analyze the significance of higher vocational nursing students participating in opening professional social practice,and to explore the effective way of combining social practice with professional learning and clinical practice.Methods After completing the opening professional social practice in summer,the reflection diaries of 30 nursing students were analyzed and the concerns were refined.Questionnaire survey about self assessment of comprehensive effect of the practice of nursing students were conducted.Results Nursing operation and soft skills were mostly concerned by nursing students' in opening professional social practice.Vocational learning,occupation of responsibility,and the occupation ability were improved by practical activities.Conclusions Nursing students who participate in opening professional social practice before clinical work,can re-understand the professional learning,adapt and integrate themselves into clinical practice through the perception of clinical nursing work.

The construction of smart library needs the support of good,multiple and effective information resources system.The necessities for information resources system in smart library were outlined in aspects of starting from the development of library holdings,sharing and open access of resources,deep revealing of resources by making full use of the discovery service system,strengthening management and assessment of resources.

Gene Expression Profiling ; Humans ; Laser Therapy ; Lung Neoplasms ; genetics ; Microdissection ; methods ; RNA, Neoplasm ; isolation & purification

To cement the 5 years’ staged achievement of new medical reform so as finally achieve the objective of medical reform, public hospital would become the aim of reform in the following stage. On one hand , canceling “using drug to supple health care” in public hospitals has came into the crucial stage; on the other hand, the government needed to increase financial input and ensure the implementation of security fund. What was the relation between these two reforms? Therefore , based on the survey of county-level public hospitals in G Province, relationship among government compensation method, hospital public profit operation mechanism and profit-driven operation mechanism were analyzed; besides, different financial insurance mode had different operation efficiency. Because the construction of hospital expense under public profit operation mechanism was more reasonable , the required government insurance fund was less, which only accounted 32.9%of the required fund under the profit-driven operation mechanism. Therefore, the policy suggestions that government need to “buy the mechanism” and “be brave to implement ‘subtraction reform’” were proposed.

Objective To establish HPLC method for the determination of the content of caffeic acid in Chanhou Tieshijin. Methods The determination was performed on HangBang C18 column (200 mm?4.6 mm, 5 ?m). The mobile phase was methanol-buffer phosphate (23∶77) at a flow rate of 1.0 mL/min. The UV detection wavelength was set at 323 nm and the sample size was 10 ?L. Results The linear range of caffeic acid was 0.052～0.26 ?g (r=0.999 8), with average recovery rate of 97.44% (RSD=0.78%). Conclusion The established method is simple, rapid and accurate, and suitable for the quality control of Chanhou Tieshijin.

Forty rats were selected for the experiment, which were divided into three groups including experimental ulcer group, normal saline and normal control groups. The pancreatic tissue were prepared for immunohistochemical staining at 6th, 10th, 14th, and 21st day after operation. Sternberger's PAP method was used to show the glucagon cell (A cell) and insulin cell (B cell) in order to observe their changes during the experimental peptic ulcer. In normal control group, the percentage of A cells is 23.31?1.91 (X?SD) and that of B cells is 73.1?4.01. But in experimental ulcer group, the A cell percentage is raised up to 42.6?5.59 and the B cell percentage is lowered down to 57.5?4.55 on the 10th day after operation. Comparing with normal saline group and normal group their differences show statistical outstanding differences. The result may point out that both A and B cells of pancreatic islet take part in the body's natural resistance against the disease, especially A cells.

Objective To explore the extent of information needs and influencing factors among breast cancer patients.Methods Choose patients by random with a questionnaire for malignant breast cancer patients(Chinese edition),and analyze the influencing factors in a multiple linear regression.Results The total extent of information needs among the patients is 65.615±20.854,with much stronger needs among those with younger age,higher income and/or suitable for surgery.Conclusion Information needs of the patients were found to depend on their various social attributes.Based on this founding,recommendations were made in terms of contents of information needs,care for vulnerable groups,and channels of information communication.

Theancientandmodernprocessingmethods,activeingredientresearch,andqualitycomparisonbeforeandafterthe processing of medicinal leech were reviewed in the paper. Combined with the results of anticoagulation test on Whitmania pigra Whit-man, the article drew a conclusion that different varieties of medicinal leech had different active ingredients of anticoagulation, Whitma-nia pigra Whitman had active ingredients of anticoagulation with promising heat stability, and the processing technology should be dealt with according to the clinic use.