Objective:To investigate the effect of pcDNA3.1 vector on house keeping gene expression and set guide to further study work in using pcDNA3.1. Methods:5 cell lines were transfected with pcDNA3.1,48 h post transfection total RNA was extracted and applied for real time PCR to detect the RNA level of GAPDH、 ?-actin、18sRNA difference.Meanwhile,extract total protein and analyze the GAPDH and?-actin expression by Western blot.Results:RNA and protein levels all show of a decrease in 5 cell lines after transfection,with the most obviously ?-actin.Conclusion:pcDNA3.1 might have non specifinhibition effect to cell,when using pcDNA3. 1 for gene function research,it is not recommend to use ?- actin as reference gene.

OBJECTIVE To compare the detection rates of Ureaplasma urealyticum(Uu),by 4 kinds rits and find out the best one.METHODS Using four kinds of mycoplasma detection kits,namely are French mycoplasmaIST2,Zhuhai Livzon′s ICS,Dark horse′s IES and Shanghai Its Prachanda,under the same conditions,the mycoplasma of the Clinical specimens for 260 cases were tested.RESULTS The detection rate of mycoplasma(Uu) for 48 hours by four kinds of brands kits about mycoplasma liquid culture was as follows 45.4% for French MycoplasmaIST2,36.2% for Zhuhai Livzon′s ICS,37.7% for Dark horse′s IES and 36.8% for Shanghai Its prachanda.The results of susceptibility test: the reagent of IST2 had.the lowest resistance to josamycin,doxycycline,clarithromycin,ofloxacin and azithromycin,and had the better results than the other three kinds of reagents.Mycoplasma reagents form different companies had significant differences in resistance rates to five kinds of antibiotics.CONCLUSIONS The results suggest that when we cultivate clinical mycoplasma should be careful to select of the mycoplasma liquid culture identification kit.

Drugs, Chinese Herbal ; administration & dosage ; standards ; Humans ; Injections, Intravenous ; Pharmaceutical Preparations ; administration & dosage ; standards ; Quality Control

Strokc is one of the most feared perioperative complications.This article systematically reviews the incidence, mechanisms, risk factors stratification,and relief of perioperative stroke.Aiming at the risk factors and pathogeneses,it is hopeful to improve from the aspect of operative methods.techniques,and medication,and thus to decrease the risks of perioperative stroke and improve prognosis.

The morbidity and mortality of intracerebral hemorrhage (ICH) are very high. Brain injury involves in several mechanisms after ICH, including the direct tissue destruction by the mechanical force on brain tissue surrounding the hernatoma in the process of hematoma formation, the release of clot-derived factors (thrombin, hemoglobin degradation products), inflammatory response and complement cascade reaction, etc. This article reviews the above mechanisms

Achievement ; Integrative Medicine ; Periodicals as Topic

Blood Circulation ; drug effects ; Cardiovascular Agents ; therapeutic use ; Cardiovascular Diseases ; prevention & control ; China ; Humans ; Medicine, Chinese Traditional

Academies and Institutes ; organization & administration ; China ; Combined Modality Therapy ; methods ; trends ; Drugs, Chinese Herbal ; therapeutic use ; Education, Medical, Continuing ; Humans ; Integrative Medicine ; legislation & jurisprudence ; trends ; International Cooperation ; Medicine, Chinese Traditional ; methods ; trends ; Western World

Critical Pathways ; Integrative Medicine

Esophageal Neoplasms ; mortality ; surgery ; Humans ; Quality of Life ; Survival Analysis