Objective:To investigate the effect of pcDNA3.1 vector on house keeping gene expression and set guide to further study work in using pcDNA3.1. Methods:5 cell lines were transfected with pcDNA3.1,48 h post transfection total RNA was extracted and applied for real time PCR to detect the RNA level of GAPDH、 ?-actin、18sRNA difference.Meanwhile,extract total protein and analyze the GAPDH and?-actin expression by Western blot.Results:RNA and protein levels all show of a decrease in 5 cell lines after transfection,with the most obviously ?-actin.Conclusion:pcDNA3.1 might have non specifinhibition effect to cell,when using pcDNA3. 1 for gene function research,it is not recommend to use ?- actin as reference gene.

Cardiovascular Diseases ; Cardiovascular System ; Diet, Vegetarian ; Humans ; Integrative Medicine ; Vegetarians

Drugs, Chinese Herbal ; Education, Medical ; Hematologic Diseases ; therapy ; Humans ; Medicine, Chinese Traditional ; Wills

China ; Humans ; Integrative Medicine ; trends

Critical Pathways ; Integrative Medicine

OBJECTIVE:To systematically review the effectiveness and safety of combination prescription of lidocaine with ropivacaine for anesthesia,and provide evidence-based reference for developing new compound preparation. METHODS:Retrieved from CJFD,Wanfang Database,VIP,PubMed and EBSCO,randomized controlled trials (RCT) about lidocaine combined with ropivacaine versus lidocaine alone or ropivacaine alone for anesthesia were collected. Meta-analysis was performed by using Rev Man 5.0 software provided by the Cochrane Collaboration after data extracting and quality evaluating. RESULTS:Totally 30 RCT were included,involving 2 442 patients. Results of Meta-analysis showed,lidocaine+ropivacaine vs. lidocaine:onset time was 6.77 min vs. 7.11 min [MD=-0.29,95%CI(-0.43,-0.16),P<0.001],maintenance time was 5.11 h vs. 2.11 h [MD=3.13,95%CI (3.02,3.25),P<0.001] and the incidence of adverse reactions was 28.6% vs. 44.6% [MD=0.43,95%CI(0.31,0.62),P<0.001];lidocaine+ropivacaine vs. ropivacaine:onset time was 9.49 min vs. 15.85 min [MD=-4.43,95%CI(-4.63,-4.22),P<0.001];maintenance time was 4.84 h vs. 6.79 h [MD=-0.61,95%CI(-0.71,-0.51),P<0.001];the incidence of adverse reac-tions was 44.8% vs. 48.1% [MD=0.82,95%CI(0.56,1.19),P=0.30]. Sensitivity analysis showed that the results were in stabili-ty. CONCLUSIONS:Lidocaine combined with ropivacaine shows rapid onset and long duration in anesthesia,and it can reduce ad-verse reactions play their respective advantages.

OBJECTIVE:To explore therapeutic efficacy and safety of Puerarin injection for elderly female patients with osteo-porosis and unstable angina pectoris,and its effects on serum inflammatory factors. METHODS:Totally 107 elderly women with osteoporosis and unstable angina were analyzed retrospectively and divided into control group(53 cases)and observation group(54 cases) according to drug use. Control group was given routine treatment as Caltrate D,Alendronate sodium tablet,Glyceryl trini-trate tablet ,Metoprolol tartrate tablet,Atorvastatin calcium tablet,Trimetazidine hydrochloride tablet. On the basis of control group, observation group was additionally given Puerarin injection 4 mL added into 5% Glucose injection 500 mL intravenously,once a day. Treatment course of 2 groups lasted for 2 weeks. Therapeutic efficacies of 2 groups were observed as well as the levels of se-rum inflammatory factors(hs-CRP,TNF-α,MMP-9,sCD40L),and the occurrence of ADR. RESULTS:The total response rates of angina pectoris and osteoporosis pain in observation group were significantly higher than control group,with statistical signifi-cance (P<0.05). Before treatment, there was no statistical significance in serum inflammatory factors as hs-CRP, TNF-α, MMP-9,sCD40L between 2 groups (P>0.05). After treatment,the levels of hs-CRP,TNF-α,MMP-9 and sCD40L in 2 groups were significantly lower than before,and the observation group was significantly lower than the control group,with statistical sig-nificance(P<0.05). No obvious ADR was found in 2 groups during treatment. CONCLUSIONS:Based on routine treatment,Pu-erarin injection shows significant therapeutic efficacy for senile female osteoporosis patients with unstable angina and can significant-ly reduce the levels of serum inflammatory factors with good safety.

Esophageal Neoplasms ; mortality ; surgery ; Humans ; Quality of Life ; Survival Analysis

Chronic Disease ; drug therapy ; prevention & control ; Humans ; Longevity ; Medicine, Chinese Traditional

Decision Making ; Esophageal Diseases ; diagnosis ; therapy ; Humans ; Physician's Role ; Thoracic Surgical Procedures