Abstract Comprehensive sexuality education is a curriculum based teaching process that explores the cognitive, emotional, physical and social aspects of sexuality and equips children and adolescents with certain knowledge, skills, attitudes and values to ensure their health, well being and dignity. Three to six years of age is an important stage for children s sexual development. Kindergarten is an important place for children to receive sexuality education. Based on the perspective of comprehensive sexuality education, sexuality education practice in kindergartens in China was analyzed, and the findings revealed the following:the policy that supports sexuality education practice in kindergartens is not comprehensive, sexuality education in kindergartens lacks teaching guidelines, kindergartens are facing a shortage of sexuality education teachers, sexuality education in kindergartens lacks the necessary family support. Based on these existing difficulties and challenges, this paper puts forward suggestions for promoting the development of sexuality education in kindergartens in consideration of the following four aspects:improving policies, formulating localized sexuality education guidelines, carrying out teacher training and strengthening publicity.