Objective To study the effect of tripterygium glycosides(TG)in treating urticaria chronica(UC).Methods 91 cases of UC in our hospital from February 2012 to March 2013 were randomly divided into the observation group(45 cases)and the con-trol group(46 cases)according to the digital method.The control group was treated with fexofenadine,while on this basis the obser-vation group was added with TG.The UAS,VAS and LFS scores before and after treatment,curative effects,serum IL-4,IFN-γand IgE levels change and adverse reactions were compared between the two groups.Results The VAS,LFS and UAS scores after treatment in the observation group were significantly lower than those before treatment and the control group after treatment;the curative effect after 4-week treatment in the observation group was significantly higher than that in the control group;the IL-4 and IgE levels after treatment in the observation group were significantly lower than those before treatment and the control group after treatment;the IFN-γlevel was significantly higher than that before treatment and the control group after treatment,all the differ-ences above were statistically significant(P<0.05).Adverse reactions had no statistically significant difference between two groups (P>0.05).Conclusion TG combined with fexofenadine in the treatment of UC has better effect.

Neonatal hypoglycemia is a common metabolic problem. The definition and clinical management of neonatal hypoglycemia remains controversial. The concerned reasons are mainly as follows:the course of a physiological decrease and recovery in the level of blood glucose is usually existed in newborn infants after birth;asymptomatic hypoglycemia(many newborn infants' blood glucose level is very low but without any clinical signs or symptoms);and the lack of the complete comprehension for the relationship between neonatal hypoglycemia and the long-term neurologic prognosis. The review describes the etiology,definition and clinical management of hypoglycemia in newborn infants.

ObjectiveTo observe the effect of probucol combined with atorvastatin in treatment of stenosis carotid atherosclerosis.MethodsEighty-six patients with stenosis carotid atherosclerosis were separated into two groups with 43 cases each by random digits table.The patients were treated with probucol combined with atorvastatin in observation group and atorvastatin only in control group.The changes of arteriosclerotic plaque and blood fat pre- and poet-treatment were observed.Results There was no significant difference in the levels of lumen area (LN) and intima-media thickness (IMT) pre-treatment between two groups (P＞ 0.05).Compared with pre-treatment,the levels of LN and IMT post-treatment were significantly decreased in observation group(P ＜ 0.05 ),while no significant difference in control group (P ＞0.05).There was significant difference in the levels of LN and IMT post-treatment between two groups [(0.59 ±0.19) cm2 vs.(0.72 ±0.28) cm2,(2.76 ±0.41 ) mm vs.(3.27 ±0.57) mm](P＜ 0.05).There was no significant difference in the levels of total cholesterol (TC),triacylglycerol (TG),low density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C) pre-treatment between two groups (P ＞0.05).Compared with pre-treatment,the levels of TC,TG,LDL-C post-treatment were decreased,HDL-C was increased,there were significant differences (P ＜ 0.05 ).Post-treatment,the levels of TC,TG,LDL-C in observation group were lower and HDL-C was higher than those in control group (P ＜ 0.05).Conclusion Probucol combined with atorvastatin in treatment of stenosis carotid atherosclerosis can improve arteriosclerotic plaque and low blood fat,which can be applied in clinic.

Objective To observe the effects of endovascular radiation (ER) on the proliferation and apoptosis of medial smooth muscle cells (SMC) and to discuss the possible mechanisms of radiation in the prevention of vascular restenosis (RS) in rabbits after carotid endarterectomy (CEA).Methods Forty rabbits undergoing CEA were randomly divided into four groups (each group=10) and given a radiation dose of 0, 10, 20 and 40 Gy 32 P respectively. Rabbits were killed on the 3rd, 7th, 14th, 28th and 56th day after operation. The specimens were collected and histopathologic examinations were done.Results Proliferation apparently occurred in the intima and media of carotid the lumen became narrow in the control group on the 14 th, 28 th and 56 th day after operation. While in the radiation groups, proliferation was apparently suppressed and the lumen was much less narrowed ( P

Objective:To investigate the clinical value of neck vascular ultrasound and homocysteine (Hcy) level in patients with cerebral infarction and carotid stenosis.
Methods: Our research included 2 groups. Cerebral infarction group, n=115 patient who were treated in our hospital from 2012-01 to 2013-12, and Control group, n=110 healthy subjects from physical check-up. Neck vascular ultrasound was conducted to classify the degree of carotid stenosis as ①no stenosis, ②mild degree, the stenosis rate≤29%, ③moderate degree, the stenosis rate at 30%~69%,③severe degree, the stenosis rate at 70%~99%,④complete occlusion. Carotid plaque formation and vascular intima-media thickness (IMT) were examined by neck ultrasound. Blood levels of Hcy and triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) were
measured in clinical laboratory.
Results: Compared with Control group, Cerebral infarction group had the higher rate of carotid stenosis 89.56%vs 10.91%, more plaque formation 73.91%vs 6.08%and increased IMT (1.85 ± 0.42) mm vs (0.75 ± 0.21) mm;higher blood levels of Hcy (42.32 ± 12.02) μmol/L vs (6.32 ± 2.45) μmol/L and higher rate of high blood Hcy syndrome 71.30%vs 3.63%;higher levels of TC (4.25 ± 1.12) mmol/L vs (1.63 ± 0.74) mmol/L and LDL-C (5.96 ± 1.36) mmol/L vs (2.32 ± 0.82) mmol/L, P<0.05. In Cerebral infarction group, the patients with complete carotid occlusion and severe stenosis had the higher rate of plaque formation and increased IMT;higher levels of Hcy and more high blood Hcy syndrome;higher levels of TG and LDL-C than the patients with mild and moderate carotid stenosis, P<0.05. Logistic analysis indicated that the levels of IMT, Hcy and LDL-C were the independent risk factors for cerebral infarction and carotid stenosis.
Conclusion: The patients with cerebral infarction and carotid stenosis have obviously increased blood level of Hcy. Hcy may predict various degree of carotid stenosis in relevant patients.

AIM:To investigate the genotype distributions of HPA-3 in Han people from Beijing and Hebei province,and to study the association of the platelet glycoprotein IIb polymorphism with coronary heart disease(CHD).METHODS:Two hundred and twelve patients with coronary heart disease and 106 healthy controls were enrolled in this case-control study.The number of occlusive coronary artery was performed in all subjects.The genotypes of HPA-3 were determined by TaqMan probe technology.RESULTS:In CHD patients(older than 45 years)carriers of HPA-3b were over-represented compared with healthy controls(P

OBJECTIVE: To investigate the differential gene expression profiles in patients with blood stasis syndrome by oligonucleotide microarray technique. METHODS: Sixteen patients with blood stasis syndrome were divided into patients with coronary heart disease (CAD) (n=8) and non-CAD patients (n=8) by using coronary angiography. The sex- and age-matched eight healthy persons were enrolled as control group. Venous bloods were collected for extracting RNA. Test-3 chip was first employed to examine the quality of samples. Then the samples were hybridized with Affymetrix U133 Plus 2.0 array to compare the gene expression profiles among the three groups. Gene-array scanner and gene chip operating software were applied to screen hybridization signals and analyze gene expression respectively. Based on the comparison of the three groups of samples, the differential genes related with blood stasis syndrome were analyzed by Gene Ontology (GO) and pathway, and confirmed by real-time reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Forty-eight differential genes were found being associated with blood stasis syndrome, including 26 up-regulated genes and 22 down-regulated genes. Five of the forty-eight genes (10.4%) were related to inflammatory reaction and immune response through the GO analysis. In the pathway analysis, five of ten significant pathways were referred to inflammation and immune response. The results of real-time RT-PCR proved the accuracy of the gene chip. CONCLUSION: Inflammatory- and immune-related genes have a remarkable predominance in blood stasis syndrome gene expression profiles, which may explain the function of inflammation and immune response in the occurrence and development of blood stasis syndrome.

To investigate the genotype distributions of PLA1/PLA2 polymorphism in Chinese Han population from Beijing and Hebei Province and to study the correlation between the platelet membrane glycoprotein IIIa polymorphism and coronary heart disease (CHD) or CHD with blood-stasis syndrome.

Objective:To observe the effect of timosaponin AⅢon the proliferation of cultured melanoma B16 and A375 cells and nitrite produced from macrophage activation. Methods:MTT assay was adopted to detect the cell growth inhibition. The morphological changes of B16 and A375 cells were observed under an inverted microscope. Nitrite production of activated mouse macrophages induced by lipopolysaccharide ( LPS) plus IFN-γ was measured by Griess assay. Results: Timosaponin AⅢ could significantly inhibit the growth of B16 cells at the concentration of 16 μmol·L-1 after the 48- and 72-hour treatment, and significantly inhibit the growth of A375 cells at the concentration ranged from 4 to 16 μmol·L-1 after the 48-and 72-hour treatment. Shrinkage, vacuoles and necrosis of B16 and A375 cells were observed after the 48-and 72-hour treatment by 16μmol·L-1 timosaponin AⅢ,the other concentration of timosaponin AⅢ showed no notable effect on B16 cells and vacuoles of A375 cells appeared at the concentration from 4 to 16 μmol· L-1. Compared with RAW 264. 7 stimulated LPS plus IFN-γ,timosaponin AⅢ could significant inhibit nitrite production of macro-phage inflammatory cell model at the concentration of 10μmol·L-1(P<0. 01). Conclusion:Timosaponin AⅢcan inhibit the prolif-eration of melanoma B16 and A375 cells and macrophage inflammation,suggesting it has anti-tumor and anti-inflammatory effects . The anti-tumor effect of timosaponin AⅢ may be related to the inhibition of tumor inflammation.

Objective To study the practicability of the Chemically-Activated Luciferase eXpresson(CALUX)bioassay used as a method for determination of dioxin in environmental epidemiological surveys.Methods The toxicity equivalents of dioxin in the same samples of body fat,blood and mothers milk were determined by high resolution gas chromatography/mass spectrometry(HRGC/MS )and the CALUX bioassay respectively and the relativity of the results obtained by the two different methods was analyzed.Results The correlation coefficients of the results of dioxin toxicity equivalents in body fat and mothers milk determined by the two methods were0.8675and0.9581respectively,which had significant positive correlation(P