1.Primary diffuse large B-cell lymphoma of the heart: a clinicopathological study.
Zheng-rong WU ; De-sheng WENG ; Yan-qing DING ; Hui-xia HAN ; Mei-gang ZHU
Journal of Southern Medical University 2006;26(10):1481-1483
OBJECTIVETo define the clinicopathological features of primary cardiac large B-cell lymphoma.
METHODA case of primary cardiac large B-cell lymphoma was studied with conventional histopathological and immunohistochemical staining in combination with literature review.
RESULTSThe lesion appeared to originate in the right atrium and involved the venae cavae and the left atrium. Microscopic examination showed diffuse proliferation of large atypical lymphocytes with abundant cytoplasm, vestiealer nuelei, thick nuclear membrane and conspicuous nucleoli. Giant tumor cells scattered in the lesion. The neoplastic cells were positive for CD20 and CD79a.
CONCLUSIONPrimary cardiac lymphoma is extremely rare, and its pathogenesis remains unclear. With non-specific clinical manifestations, the majority of primary cardiac lymphomas are of B-cell lineage and a bad prognosis.
Aged ; Antigens, CD20 ; analysis ; CD79 Antigens ; analysis ; Female ; Heart Neoplasms ; metabolism ; pathology ; Humans ; Lymphoma, Large B-Cell, Diffuse ; metabolism ; pathology
3.Burkitt's lymphoma of the spermatic cord: a case report and review of the literature.
Qian ZHOU ; Dong-Ni LENG ; Zheng-Xiang ZHANG ; Hang-Bo ZHOU ; Qun-Li SHI ; Xiao-Jun ZHOU
National Journal of Andrology 2008;14(7):624-627
OBJECTIVETo investigate the clinicopathological characteristics of primary Burkitt's lymphoma (BL) in the spermatic cord.
METHODSA case of BL of the spermatic cord was studied by histopathology and immunohistochemical techniques. The clinical data and the related literature were reviewed.
RESULTSThe patient was a 4-year-old boy, who was accidentally found with a bump in the scrotum. Surgery showed it to be a tumor located in the left spermatic cord and 5 cm x 3 cm x 2 cm in size, gray and fish-like on cross-sectional imaging. Histologically, it was characterized by monotonous infiltration of medium-sized cells with round nuclei, coarse chromatin, 2-5 basophilic nucleoli, and an appreciable rim of basophilic cytoplasm, in a typically starry-sky pattern imparted by interspersed tangible-body macrophages. Immunohistochemically, the tumor cells were diffused, positive for CD20 and CD79, some for CD10 and about 95% with the nuclear expression of Ki-67, but negative for CD3, CD43, bcl-2 and TdT as well as for EBER in situ hybridization.
CONCLUSIONPrimary spermatic cord BL is extremely rare, highly aggressive and with poor prognosis. Diagnosis of the tumor relies on its pathological characteristics and immunohistochemical staining. It is essential to differentiate BL from other types of lymphomas and malignant small-cell tumors of the non-lymphatic system.
Antigens, CD20 ; analysis ; Burkitt Lymphoma ; metabolism ; pathology ; CD79 Antigens ; analysis ; Child, Preschool ; Genital Neoplasms, Male ; metabolism ; pathology ; Humans ; Immunohistochemistry ; Ki-67 Antigen ; analysis ; Male ; Neprilysin ; analysis ; Spermatic Cord
4.Testicular plasmacytoma: a case report and review of the literature.
Yi-min WANG ; Fang-yin LI ; Jin-dan LUO ; Jun LI ; Li-ping XIE ; Guo-sheng YANG
Chinese Medical Journal 2008;121(10):956-958
CD79 Antigens
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analysis
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Humans
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Immunohistochemistry
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Male
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Middle Aged
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Mucin-1
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analysis
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Plasmacytoma
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metabolism
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pathology
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Testicular Neoplasms
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metabolism
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pathology
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Testis
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chemistry
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pathology
5.Application of a four antibody (cMPO/cCD79aalpha/cCD3/CD45) combination to the diagnosis of acute leukemia expressing cross-lineage antigens.
Yu-Jie WU ; Jian-Yong LI ; Ming-Qing ZHU ; Jun-Hong SONG ; Wen-Juan ZHENG
Chinese Journal of Hematology 2006;27(7):449-451
OBJECTIVETo explore the diagnostic value of intracellular antibody combination in acute leukemia (AL) expressing cross-lineage cell-surface antigens.
METHODSFlow cytometric immunophenotyping using intracellular antibody combination (cMPO/cCD79alpha/cCD3/CD45) was performed additionally in 60 patients who expressed cross-lineage antigens from 269 previously untreated adult AL.
RESULTSFifty-four of 269 previously untreated adult AL patients who expressed only one kind of intracellular antigen were diagnosed as cross-lineage AL, the percentage of cross-lineage AL in T cell acute lymphoblastic leukemia (T-ALL), B-ALL and acute myeloid leukemia (AML) was 28.6%, 43.6% and 13.4%, respectively. The positive rate of CD7, CD19, CD5 and CD20 in cross-lineage AML was 65.4%, 15.4%, 11.5%, and 7.7%, respectively. The positive rate of CD13, CD33 and CD15 in cross-lineage ALL was 89.3%, 21.4% and 3.6%, respectively. Six (2.3%) patients expressed two-lineage intracellular antigens were diagnosed as biphenotypic AL: 2 of T/B type and 4 B/M (B/myeloid) type.
CONCLUSIONIntracellular antibodies possess lineage specificity and four-color combination flow cytometric immunophenotyping can provide fast and multi-parameter data. To ensure accuracy of the results, CD45/SSC gating and normal cells as internal reference should be used in the immunophenotyping of abnormal cells.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antibodies, Monoclonal ; Antigens, Surface ; immunology ; CD3 Complex ; immunology ; CD79 Antigens ; immunology ; Cross Reactions ; Female ; Flow Cytometry ; Humans ; Immunophenotyping ; Leukocyte Common Antigens ; immunology ; Male ; Middle Aged ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; diagnosis ; immunology ; Sensitivity and Specificity
6.Identification of Gene Expression Signatures in Korean Acute Leukemia Patients.
Kyung Hun LEE ; Se Won PARK ; Inho KIM ; Sung Soo YOON ; Seonyang PARK ; Byoung Kook KIM
Genomics & Informatics 2006;4(3):97-102
BACKGROUND: In acute leukemia patients, several successful methods of expression profiling have been used for various purposes, i.e., to identify new disease class, to select a therapeutic target, or to predict chemo-sensitivity and clinical outcome. In the present study, we tested the peripheral blood of 47 acute leukemia patients in an attempt to identify differentially expressed genes in AML and ALL using a Korean-made 10K oligo-nucleotide microarray. METHODS: Total RNA was prepared from peripheral blood and amplified for microarray experimentation. SAM (significant analysis of microarray) and PAM (prediction analysis of microarray) were used to select significant genes. The selected genes were tested for in a test group, independently of the training group. RESULTS: We identified 345 differentially expressed genes that differentiated AML and ALL patients (FWER < 0.05). Genes were selected using the training group (n=35) and tested for in the test group (n=12). Both training group and test group discriminated AML and ALL patients accurately. Genes that showed relatively high expression in AML patients were deoxynucleotidyl transferase, pre-B lymphocyte gene 3, B-cell linker, CD9 antigen, lymphoid enhancer-binding factor 1, CD79B antigen, and early B-cell factor. Genes highly expressed in ALL patients were annexin A 1, amyloid beta (A4) precursor protein, amyloid beta (A4) precursor-like protein 2, cathepsin C, lysozyme (renal amyloidosis), myeloperoxidase, and hematopoietic prostaglandin D2 synthase. CONCLUSION: This study provided genome wide molecular signatures of Korean acute leukemia patients, which clearly identify AML and ALL. Given with other reported signatures, these molecular signatures provide a means of achieving a molecular diagnosis in Korean acute leukemia patents.
Amyloid
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Antigens, CD79
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Antigens, CD9
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B-Lymphocytes
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Cathepsin C
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Diagnosis
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DNA Nucleotidylexotransferase
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Gene Expression*
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Genome
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Humans
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Leukemia*
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Leukemia, Myeloid, Acute
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Lymphoid Enhancer-Binding Factor 1
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Muramidase
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Peroxidase
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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Precursor Cells, B-Lymphoid
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Prostaglandin D2
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RNA
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Transcriptome*
8.Individualized leukemia cell-population profiles in common B-cell acute lymphoblastic leukemia patients.
Jian-Hua YU ; Jing-Tao DONG ; Yong-Qian JIA ; Neng-Gang JIANG ; Ting-Ting ZENG ; Hong XU ; Xian-Ming MO ; Wen-Tong MENG
Chinese Journal of Cancer 2013;32(4):213-223
Immunophenotype is critical for diagnosing common B-cell acute lymphoblastic leukemia (common ALL) and detecting minimal residual disease. We developed a protocol to explore the immunophenotypic profiles of common ALL based on the expression levels of the antigens associated with B lymphoid development, including IL-7Rα (CD127), cytoplasmic CD79a (cCD79a), CD19, VpreB (CD179a), and sIgM, which are successive and essential for progression of B cells along their developmental pathway. Analysis of the immunophenotypes of 48 common ALL cases showed that the immunophenotypic patterns were highly heterogeneous, with the leukemic cell population differing from case to case. Through the comprehensive analysis of immunophenotypic patterns, the profiles of patient-specific composite leukemia cell populations could provide detailed information helpful for the diagnosis, therapeutic monitoring, and individualized therapies for common ALL.
Adult
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Antigens, CD19
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metabolism
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B-Lymphocytes
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immunology
;
metabolism
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CD79 Antigens
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metabolism
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Female
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Humans
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Immunoglobulin Light Chains, Surrogate
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metabolism
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Immunophenotyping
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Male
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Middle Aged
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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immunology
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pathology
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Receptors, Interleukin-7
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metabolism
9.Intravascular large B-cell lymphoma: report of two autopsy cases with literature review.
Xia XU ; Zong-guo PANG ; Wei-ping LIU ; Shang-fu ZHANG ; Yuan TANG ; Dian-ying LIAO ; Gan-di LI
Chinese Journal of Pathology 2008;37(6):377-383
OBJECTIVETo study the clinicopathologic features of intravascular large B-cell lymphoma (IVLBCL).
METHODSTwo autopsy cases of IVLBCL were retrieved from the archival file. The clinicopathologic features, immunohistochemistry and molecular findings were studied.
RESULTSThe deceased were 70-year-old and 50-year-old males. Both of them had complained of a sudden onset of weakness and numbness of lower extremities. The clinical course deteriorated rapidly, with multi-organ failure. They died 85 days and 44 days after the presentation, respectively. Post-mortem examination did not reveal any mass lesion, except the presence of multiple skin and epicardium nodules, ranging from 0.5 cm to 2.5 cm in diameter, in the first patient. Pericardial effusion, ascites and pleural effusion were also observed. Histologically, neoplastic lymphoid cells filled up the small vessel lumina in many organs, including brain, hypophysis, spinal cord, spinal nerve roots, heart, lungs, kidneys, liver, spleen, digestive tract, pancreas, adrenal, thyroid, testes and lymph nodes. The tumor cells were relatively monotonous and of medium to large in size with round vesicular nuclei and 1 to 3 small basophilic nucleoli. Immunohistochemical study showed that the lymphoma cells expressed B-cell markers CD20 and CD79a, occasionally positive for CD5 and bcl-2 but negative for CD3, bcl-6, CD10, CD30, myeloperoxidase and cytokeratin. In-situ hybridization for Epstein-Barr virus-encoded RNA was negative. The proliferative index, as demonstrated by Ki-67 staining, was about 80%. Molecular study showed the presence of immunoglobulin heavy chain gene rearrangement in both cases, T-cell receptor-gamma gene rearrangement was not found.
CONCLUSIONSIVLBCL may present as neurological disturbance and carries distinctive morphologic characteristics, immunophenotype and molecular findings. The prognosis of this disease is often dismal.
Aged ; Antigens, CD20 ; analysis ; Autopsy ; B-Lymphocytes ; pathology ; virology ; CD79 Antigens ; analysis ; Herpesvirus 4, Human ; isolation & purification ; Humans ; Immunohistochemistry ; Lymphoma, B-Cell ; immunology ; pathology ; virology ; Lymphoma, Large B-Cell, Diffuse ; immunology ; pathology ; virology ; Male
10.Primary cardiac lymphoma of non-Hodgkin's lymphoma located in the right atrium: report of a case.
Yong-li GAN ; Xiang-lei HE ; Ya-jun RUAN
Chinese Journal of Pathology 2007;36(5):355-356
Antigens, CD20
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metabolism
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CD79 Antigens
;
metabolism
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Heart Atria
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Heart Neoplasms
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diagnostic imaging
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metabolism
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pathology
;
surgery
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Humans
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Immunohistochemistry
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Lymphoma, Large B-Cell, Diffuse
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diagnostic imaging
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metabolism
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pathology
;
surgery
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Male
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Middle Aged
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Tomography, X-Ray Computed