1.Roles of CEBPA mutation and expression abnormality in acute myeloid leukemia - review.
Li-Mengmeng WANG ; Hao-Wen XIAO ; He HUANG
Journal of Experimental Hematology 2012;20(5):1256-1260
CCAAT enhancer binding protein A (CEBPA) and its product transcription factor CCAAT enhancer binding protein α (C/EBPα) play pivotal roles in early granulocyte development. C/EBPα induces the transition and keeps the balance of differentiation and proliferation of myeloid progenitors. The mutation and dysregulation of CEBPA at transcription, translation or post-translation level lead to differentiation block and over proliferation of immature hematopoietic cells, which are important mechanisms of acute myeloid leukemia (AML). The mutation and dysregulation of CEBPA also provide clues for evaluating the outcome of AML patients and potential targets for differentiation-inducing therapies. This review focus on CEBPA mutation and AML, dysregulation of C/EBPα protein expression and AML, as well as C/EBPα protein and targeting therapy.
CCAAT-Enhancer-Binding Proteins
;
genetics
;
metabolism
;
Humans
;
Leukemia, Myeloid, Acute
;
genetics
;
metabolism
;
Mutation
3.Expression and significance of C/EBPα and CK10 in nasal inverted papilloma.
Yibing YAUN ; Xianying MENG ; Xu WU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(5):437-440
OBJECTIVE:
The expression of C/EBPα, CK10 in nasal inverted papilloma (NIP) were detected in the study. Further discussed their significance in genesia, development and recurrence of NIP.
METHOD:
Three groups including nasal cavity mucosae (NM 10 cases), nasal polyp (NP 20 cases) and NIP (30 cases) were selected in the study. Expretion of C/EBPα, CK10 were detected by immunohistochemisty PV-6000 method.
RESULT:
(1) The different expression of C/EBPα and CK10 in the group of NM, NP and NIP was statistically significant (P < 0.05). (2) The different expression of C/EBPα, CK10 in the group of benign NIP and NIP with atypical hyperplasia was statistically significant (P < 0.05). (3) The different expression of C/EBPα and CK10 in the group of NIP with recurrence and NIP with no recurrence was statistically significant, P < 0.05, respectively. (4) Our result indicate that the relationship of C/EBPα and CK10 (r = 0.578, P < 0.01) was direct correlation. The difference was statistically significant.
CONCLUSION
In conclusion, the present results describe C/EBPα, CK10 expression in NIP and their possible implication in the regulation of tumor growth and differentiation. C/EBPα and CK10 production may prove useful in terms of a prognostic marker for the recurrence in nasal inverted papilloma.
CCAAT-Enhancer-Binding Proteins
;
genetics
;
metabolism
;
Humans
;
Keratin-10
;
genetics
;
metabolism
;
Nasal Polyps
;
genetics
;
metabolism
;
Neoplasm Recurrence, Local
;
Nose
;
Nose Neoplasms
;
genetics
;
metabolism
;
Papilloma, Inverted
;
genetics
;
metabolism
4.Clinical study of correlation between the expression of ICBP90 and hematopoietic suppression in patients with chronic benzene poisoning.
Sheng LUO ; Xiao-yan HUANG ; Qian HU ; Xu-dong HU ; Chong-yun XING ; Kang YU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2012;30(8):571-574
OBJECTIVETo observe the change of ICBP90 expression in patients with chronic benzene poisoning and explore the correlation between the expression of ICBP90 and benzene-induced hematotoxicity.
METHODSThe bone marrow samples were from 13 chronic benzene poisoning cases with hematopoietic suppression, 11 chronic benzene poisoning cases with hematopoietic regeneration and 10 controls. Western-blot was applied to detect the ICBP90 expression in bone marrow mononuclear cells (BMNCs). The correlation between ICBP90 expression and hematopoietic suppression in patients with chronic benzene poisoning was analyzed.
RESULTSThe ICBP90 expression of BMNCs in 13 chronic benzene poisoning cases with hematopoietic suppression was significantly lower than that in controls (P < 0.01). The ICBP90 expression of BMNCs in 11 chronic benzene poisoning cases with hematopoietic regeneration was significantly higher than those in controls and 13 chronic benzene poisoning cases with hematopoietic suppression (P < 0.05 or P < 0.01), respectively. There were good correlations between the expression of ICBP90 and white blood cell and platelet counts in patients with chronic benzene poisoning (r(1) = 0.555,P = 0.006; r(2) = 0.854,P < 0.01).
CONCLUSIONThe ICBP90 expression of BMNCs in the chronic benzene poisoning cases with hematopoietic suppression decreased significantly, and the ICBP90 expression of BMNCs in the chronic benzene poisoning cases with hematopoietic regeneration increased significantly. There was good correlation between hematopoietic suppression and ICBP90 expression in patients with chronic benzene poisoning.
Adult ; Benzene ; poisoning ; Blood Platelets ; metabolism ; Bone Marrow Cells ; metabolism ; CCAAT-Enhancer-Binding Proteins ; metabolism ; Case-Control Studies ; Female ; Hematopoiesis ; drug effects ; Humans ; Leukocytes ; metabolism ; Male ; Young Adult
5.The CEBPE rs2239633 genetic polymorphism on susceptibility to childhood acute lymphoblastic leukemia: an updated meta-analysis.
Liu JIN ; Weiling GU ; Xueqin LI ; Liang XIE ; Linhong WANG ; Zhongwen CHEN
Environmental Health and Preventive Medicine 2021;26(1):2-2
OBJECTIVES:
We performed an updated meta-analysis to clarify the relationship between the CEBPE rs2239633 polymorphism and the childhood acute lymphoblastic leukemia (CALL) susceptibility.
METHODS:
All the case-control studies were updated on October 5, 2020, through Web of Science, PubMed, Cochrane Library, Embase, and China National Knowledge Infrastructure (CNKI) electronic database. The heterogeneity in the study was tested by the Q test and I
RESULTS:
A total of 20 case-control studies were selected, including 7014 patients and 16,428 controls. There was no association of CEBPE rs2239633 polymorphism with CALL (CC vs CT + TT: OR = 1.08, 95% CI = 0.94-1.26; CC + CT vs TT: OR = 1.10, 95% CI = 0.94-1.30; C vs T: OR = 1.02, 95% CI = 0.92-1.13). In the subgroup analysis by ethnicity, there is no significant association of this polymorphism and CALL risks among Asian and Caucasian populations in the three genetic models (CC vs CT + TT, CC + CT vs TT, and C vs T).
CONCLUSION
This meta-analysis found no significant association between the CEBPE rs2239633 polymorphism and susceptibility to CALL.
Adolescent
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CCAAT-Enhancer-Binding Proteins/metabolism*
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Child
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Child, Preschool
;
Genetic Predisposition to Disease/genetics*
;
Humans
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Infant
;
Polymorphism, Single Nucleotide
;
Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics*
6.Effects of chrysophanol on expression of SREBPs and lipid metabolism in Huh-7 cells.
Jin-Mei LI ; Li-Li DING ; Bao-Liang SONG ; Li YANG ; Zheng-Tao WANG
Acta Pharmaceutica Sinica 2015;50(2):174-179
Rhubarb is a traditional Chinese medicines which possess laxative, lipid-lowering, and weight-loss activities, but the active compounds of lipid-lowering and underlying molecular mechanisms are not yet clear. This study aims to explore the effects of chrysophanol on the mRNA expressions of sterol regulatory element-binding proteins (SREBPs) and lipid metabolism in human liver carcinoma Huh-7 cells, which is one of the active compounds obtained from Rhubarb. A reporter gene assay was used to test the transcription of SREBP. The intracellular triglyceride and total cholesterol contents were measured by using commercially available test kits. The SREBPs target genes expressions were measured by Quantitative Real-Time PCR. Cell viability was evaluated by Cell Counting Kit-8. As the results shown, chrysophanol (40 μmol · L(-1), 16 h) could notably inhibited human SRE promoter activity in a dose-dependent manner and decrease intracellular cholesterol and triglyceride levels. Furthermore, the mRNA expressions of SREBPs target genes were significantly downregulated by chrysophanol treatment. However there are no significant differences on cell viability when compared with the control group. These results suggested that chrysophanol might improve lipid metabolism through suppressing the mRNA expressions of SREBPs target genes to attenuate intracellular lipid accumulation.
Anthraquinones
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pharmacology
;
CCAAT-Enhancer-Binding Proteins
;
Cell Line, Tumor
;
drug effects
;
Cholesterol
;
analysis
;
Down-Regulation
;
Gene Expression
;
Genes, Reporter
;
Humans
;
Lipid Metabolism
;
drug effects
;
Promoter Regions, Genetic
;
Sterol Regulatory Element Binding Proteins
;
pharmacology
;
Triglycerides
;
analysis
7.Identification and validation of novel C/EBPbeta-regulated genes in preadipocyte proliferation.
Chinese Medical Journal 2010;123(9):1190-1194
BACKGROUNDCCAAT/enhancer-binding protein beta (C/EBPbeta) is required for mitotic clonal expansion (MCE) during adipogenesis. It is still unclear how C/EBPbeta regulates MCE in the earlier differentiation programs of 3T3-L1 preadipocytes. The purpose of this paper was to understand why C/EBPbeta is required for preadipocyte proliferation, and identify new target genes of C/EBPbeta with chromatin immunoprecipitation (ChIP)-on-chip.
METHODSPostconfluent growth-arrested 3T3-L1 preadipocytes were induced to differentiation using a standard differentiation protocol. ChIP was performed at 20 hours after induction with specific anti-C/EBPbeta antibodies. The precipitated DNA was amplified, labeled and hybridized with a mouse promoter microarray. Compared with the control in which the ChIP experiment was performed with non-specific antibody, only the genes with a signal increasing more than 2 fold were considered as candidate genes.
RESULTSA total of 110 candidate genes were identified. BTG3 associated nuclear protein (SMAR1, Banp) and tripartite motif-containing 35 (Hls5, trim35) were two target genes among the 110 candidate genes which are involved in cell cycle regulation; the binding of C/EBPbeta to the promoter of banp and trim35 was verified by ChIP-PCR.
CONCLUSIONC/EBPbeta may regulate preadipocyte proliferation through activation of banp and trim35.
3T3-L1 Cells ; Adipocytes ; cytology ; Animals ; Apoptosis Regulatory Proteins ; genetics ; metabolism ; CCAAT-Enhancer-Binding Protein-beta ; genetics ; metabolism ; Cell Cycle Proteins ; genetics ; metabolism ; Cell Differentiation ; genetics ; physiology ; Cell Proliferation ; Chromatin Immunoprecipitation ; DNA-Binding Proteins ; genetics ; metabolism ; Mice ; Nuclear Proteins ; genetics ; metabolism ; Oligonucleotide Array Sequence Analysis ; Polymerase Chain Reaction ; Protein Binding
8.C/EBP binding activity to site F of the rat GLUT2 glucose transporter gene promoter is attenuated by c-Jun in vitro.
Experimental & Molecular Medicine 2002;34(5):379-384
The expression of the GLUT2 glucose transporter gene in liver is suppressed in cultured hepatoma cell lines and primary cultured hepatocytes. Earlier report showed that CCAAT/enhancer binding protein (C/EBP) regulates the promoter activity of the rat GLUT2 glucose transporter gene in liver cells. C/EBPa and C/EBPb activated the promoter activity by binding to at least two regions of the promoter and one of the C/EBP binding sites, named as site F, also has the AP-1 binding consensus. In this study, we investigated whether the AP-1 can influence on C/EBP binding to this site. The addition of recombinant c-Jun protein with liver extract caused the attenuation of C/EBP binding to site F with the appearance of a new shifted band. The shifted band was competed out with the addition of unlabeled AP-1 consensus oligonucleotide, indicating that c-Jun also can bind to site F. Another C/EBP site on GLUT2 promoter, site H, did not bind AP-1. Analysis of the DNA-protein complex revealed that C/EBP and c-Jun bind to site F in mutually exclusive manner rather than form heterodimeric complex with each other. From these results, it is suggested that the transcriptional activation of C/EBP may be influenced by c-Jun protein in certain status of the liver cells, such as acute phase response, as well as hepatocarcinogenesis.
Animals
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Base Sequence
;
Binding Sites
;
CCAAT-Enhancer-Binding Proteins/*metabolism
;
Cell Nucleus/metabolism
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Cells, Cultured
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Liver/cytology/metabolism
;
Male
;
Molecular Sequence Data
;
Monosaccharide Transport Proteins/*genetics/metabolism
;
Promoter Regions (Genetics)/*physiology
;
Proto-Oncogene Proteins c-jun/genetics/*metabolism
;
Rats
;
Rats, Sprague-Dawley
;
Recombinant Proteins/genetics/metabolism
;
Transcription Factor AP-1/genetics/metabolism
9.Human LZIP induces monocyte CC chemokine receptor 2 expression leading to enhancement of monocyte chemoattractant protein 1/CCL2-induced cell migration.
Ho Joong SUNG ; Yoon Suk KIM ; Hyereen KANG ; Jesang KO
Experimental & Molecular Medicine 2008;40(3):332-338
Chemokines and chemokine receptors play a role in migration of circulating leukocytes to the region of inflammation. Human LZIP is an uncharacterized transcription factor and is known to participate in leukotactin (Lkn)-1/CCL15-induced cell migration. We investigated the role of human LZIP in expression of CC chemokine receptors (CCRs) and its involvement in monocyte migration. RNase protection analysis showed that LZIP increased mRNA expression of CCR2 and CCR1 in THP-1 cells. Surface expressions of both CCR2 and CCR1 were also increased by LZIP. Results from an electrophoretic mobility shift assay showed that LZIP binds to the C/EBP element in the CCR2 promoter. LZIP also enhanced the chemotactic activities of monocyte chemoattractant protein-1/CCL2 and Lkn-1. These results suggest that LZIP regulates expression of chemokine receptors that are involved in monocyte migration.
Atherosclerosis/drug therapy/etiology
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CCAAT-Enhancer-Binding Proteins/genetics/immunology/*metabolism
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Cell Line, Tumor
;
Cell Movement/drug effects/*physiology
;
Chemokine CCL2/*pharmacology
;
Chemokines, CC/pharmacology
;
Cyclic AMP Response Element-Binding
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Humans
;
Macrophage Inflammatory Proteins/pharmacology
;
Monocytes/drug effects/metabolism
;
Promoter Regions, Genetic
;
Protein Binding
;
RNA, Messenger/analysis
;
Receptors, CCR1/biosynthesis/genetics
;
Receptors, CCR2/*biosynthesis/genetics
;
Transcriptional Activation/drug effects
;
Transfection
;
Transgenes
10.CCAAT/enhancer binding proteins play a role in oriLyt-dependent genome replication during MHV-68 de novo infection.
Jing QI ; Danyang GONG ; Hongyu DENG
Protein & Cell 2011;2(6):463-469
Murine gammaherpesvirus 68 (MHV-68), a member of the gammaherpesvirus family, replicates robustly in permissive cell lines and is able to infect laboratory mice. MHV-68 has emerged as a model for studying the basic aspects of viral replication and host-virus interactions of its human counterparts. Herpesvirus genome replication is mediated through a cis-element in the viral genome called the origin of lytic replication (oriLyt). A family of transcription factors, CCAAT/enhancer binding proteins (C/EBPs), assists in oriLyt-mediated DNA replication during gammaherpesvirus reactivation. In this study, we examined the role of C/EBPs in gammaherpesvirus DNA replication during de novo infection, using MHV-68 as a model. We found that C/EBP α and β bind to the CCAAT boxes in the MHV-68 oriLyt core region both in vitro and in vivo, as demonstrated by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. A dominant negative form of C/EBPs significantly impaired the lytic replication efficiency of MHV-68 on both the plasmid and genome levels in a replication assay, indicating that functional C/EBPs are required for maximal MHV-68 genome DNA replication. Collectively, our data demonstrate that C/EBPs interact with the oriLyt core region and play an important role in MHV-68 lytic DNA replication during de novo infection.
Animals
;
Base Sequence
;
CCAAT-Enhancer-Binding Proteins
;
genetics
;
metabolism
;
Cell Line
;
Chromatin Immunoprecipitation
;
Cricetinae
;
DNA Replication
;
DNA, Viral
;
chemistry
;
genetics
;
metabolism
;
Electrophoretic Mobility Shift Assay
;
Genome, Viral
;
Herpesviridae Infections
;
genetics
;
metabolism
;
virology
;
Humans
;
Mice
;
Molecular Sequence Data
;
Plasmids
;
Promoter Regions, Genetic
;
Protein Isoforms
;
genetics
;
metabolism
;
Replication Origin
;
Rhadinovirus
;
genetics
;
metabolism
;
Viral Proteins
;
genetics
;
metabolism
;
Virus Latency
;
genetics