Kawasakd disease(KD) or incomplete KD(IKD) is a systemic vasculitis predominantly affecting young children.Clinical manifestations include fever with no obvious origin,skin damage,abnormalities of mucous membrane and lymph nodes.The disease is often accompanied by serious cardiovascular complications such as coronary artery aneurysm,therefore it attracts more and more attention.But arthritis or arthralgia complicates over one-third of KD or IKD patients.So early-onset arthritis is often indistinguishable from early juvenile idiopathic arthritis,especially systemic-onset juvenile idiopathic arthritis(SoJIA).KD and SoJIA are considered to be different diagnoses for children with long-term fever,rash,swollen lymph nodes,and more importantly,the treatment procedures are very different,but it is difficult to completely distinguish the two diseases,especially the IKD and early SoJIA.So in this paper,we will summarize the relationship between KD and arthritis in children,which aims to remind clinicians to pay attention for the diagnosis of KD and IKD.We suggest that physicians should be alert of the fact that they must individualize every patient's management,rather than merely care by the guidelines,which will delay the treatment.

AIM: To investigate the concentration of bufadienolides in Liushen Pill from two enterprises. METHODS: Four kinds of bufadienolides were simultaneously determined by using HPLC with DAD detector. RESULTS: Recoveries obtained from the four kinds of bufadienolides were higher than 96%,the linearity was in the range of 1 ?g/mL-100 ?g/mL.The concentration of bufadienolides in Liushen Pill was significantly higher than that in other related TCM compatibilities. CONCLUSION: The well-distributed compatibility in Liushen Pill has an influence on the solubilization of bufadienolides,may be in relation to eahancing to release.

FPGA is a kind of powerful programmable chip used in the development of digital system, and it has such advantages as large capacity, reliability and easy modification. The design of FPGA system is mainly through software method which is easy to grasp. Compared with legacy discrete circuit or ASIC, more and more FPGAs are adopted in digital systems for low cost and fast speed. On the other hand, digital system plays an important role in control system of medical equipments, so FPGA is expected to be widely used in almost all fields of medical equipment.

Objective To explore the effects of TMPRSS2 promoted suicide gene CD-5-FC combined with phytochemicals on the proliferation and apoptosis of prostate cancer (PCa) specific cell-line 22RV1.Methods From March 2016 to October 2016 TMPRSS2-VISA-CD/UPRT'vector was used to deliver pro-drug 5-FC (5-Fluorocytosine) in to PCa specific cell-line 22RV1.Transfection effect was verified by Western-blotting.5-FC,5-FU (5-Fluorouracil) and four phytochemicals epigallocatechin gallate (ECGC),Genistein,Daidzein,and Equol were selected in this study.MTS assay was performed to select dosages,which can kill 10％-40％ of 22RV1 cells,from their pre-set drug concentrations,respectively.According to their select concentrations,either 5-FC plasmid or 5-FU in combination with one of four phytochemicals were added in 22RV1 cells in orders,and then cultured them together.Besides,these four phytochemicals were added in 22RV1 cells individually regarding their selecting concentrations and cultured at the same time.Cell viability was detected by MTS assay on 24hrs,48hrs and 72hrs.Comparing the cell killing rates between the combination groups and each single drug groups on cell-line 22RV1.Results TMPRSS2-VISA-CD/UPRT'plasmid with 5-FC was transfected on PCa specific cell-line 22RV1 successfully.The most obvious transfection occurred on 48hrs.The selected concentration of the pro-drug 5-FC were 83.32 μmol/L and 833.20 μmol/L,5-FU were 76.87 mol/L and 768.70 μmol/L.The concentrations of four phytochemical agents were 2.00 μmol/L,10.00 μmol/L and 20.00 μmol/L.In term of the cell killing numbers,results showed that the combination treatment of 83.32 μmol/L 5-FC/ 76.87μmol/L 5-FU and 10.00 μmol/L/20.00 μmol/L four phytochemicals compared with 10.00 μmol/L/20.00 μmol/L single treatment of four phytochemicals,respectively,there was no significant difference (P ＞0.05),while the combination treatment of 833.20 μmol/L 5-FC/768.70 μ mol/L 5-FU and 2.00 μmol/L/10.00 μmol/L four phytochemicals compared with 2.00 μ mol/L/10.00 μmol/L single treatment of four phytochemicals,respectively,there was a significant difference (P ＜ 0.05).In addition,this study proved that 83.32 μmol/L 5-FC and 76.87 μmol/L 5-FU,833.20 μmol/L 5-FC and 768.70 μmol/L 5-FU reached the same cell killing effect,and there was no statistical difference (P ＞ 0.05).Thus the suicide gene transfection was successful,and its function was the same as the effect of cytotoxic drugs.Conclusions This study has proved that this plasmid with 5-FC suicide gene system can be successfully and efficiently transfected into PCa cells 22RV1.As the pro-drug of 5-FU,5-FC got similar treatment effect with 5-FU,and inhibited cell proliferation.There was no synergistic reaction of enhancing cell apoptosis after combined phytochemical drugs EGCG,Genistein,Daidzen,Equol with suicide gene therapy on 22RV1 cell-line.

Objective To set up a method for determining the content of anthraquinone in Liuwei Nengxiaosan.Methods The chromatogram column was Eclipse XDB C18 (150 mm×4.6 mm, 5μm), the mobile phase composition was methanol-0.1% orthphosphoric acid (88∶12), the detection wavelength was at 254 nm, the column temperature was 30℃, the flow rate was 1.0 mL/min. Results The linear range of aloe emodin, rhein, emodin, chrysophanol and physcion was 0.076-0.380μg (r=0.999 6), 0.068-0.340μg (r=0.999 8), 0.076-0.380μg (r=0.999 9), 0.070-0.349μg (r=0.999 9), 0.071-0.355μg (r=0.999 7), respectively. The average recovery rate respectively was 97.88% (RSD=0.72%), 97.52% (RSD=0.96%), 98.79% (RSD=0.95%), 97.77% (RSD=1.18%), 96.34% (RSD=2.00%).Conclusion The method is highly effective, quick and sensitive, suitable for quality control of Tibet traditional medicine Liuwei Nengxiaosan.

Objective: To further optimize the hospital inspection process, shorten the patient waiting time of examination and improve the overall utilization of medical equipment. Methods:HIS and PACS to realize the information system integration by using hospital information platform, and then develop a set of medical equipment reservation system. The doctor workstation through the system could real-time help patient appoint check items, and eliminate patients to check the front desk or reservation center reservation registration steps. Results:The successful application of medical equipment reservation system not only optimize hospital inspection process, shorten the patient waiting in line inspection time, but also increase the utilization rate of MR equipment by 35%. Conclusion: The use of medical equipment reservation system saves the patient queuing registration, waiting for the examination of the time, and improve the utilization rate of hospital medical equipment, create economic benefits for the hospital and brought widespread popularity and reputation for the hospital.

Objective To investigate the changes of platelet activating factor ( PAF) in serum from patients with hypertensive disorder complicating pregnancy .Methods The specimens from 138 women were obtained at Department of Obstetrics , Xinhua Hospital , School of Medicine , Shanghai Jiaotong Uni-versity, between June 2008 and March 2010.They were divided into two groups:women( n =63) with hy-pertensive disorder complicating pregnancy and normal pregnant women ( n =75 ) ( control group ) .All of them delivered until maturity .ELISA was used to detect the levels of PAF in those women's serum and in the umbilical cord serum .Results The levels of PAF in hypertensive disorder complicating pregnancy group (27.46 ±13.37 ) ng/ml were no significantly different from the control group (25.48 ±11.05 ) ng/ml( P >0.05 ) .With the hypertensive disorder complicating pregnancy more deteriorative , the levels of women ser-um PAF would be higher .The levels of women's serum PAF in hypertensive disorder complicating pregnancy group were significantly higher than the umbilical cord serum [0.00(0.00-8.83) ng/mL]( P <0.05). The levels of women's serum PAF in control group were also significantly higher than the umbilical cord ser -um[0.00(0.00-7.80)ng/mL]( P <0.05).Conclusion There are some relationships between PAF and hypertensive disorder complicating pregnancy .

BACKGROUND:Mesenchymal stem cells from transgenic animals carry green fluorescent protein that can be stably expressed in viable cells and can be used to rapidly screen modified cells in comparison with traditional virus and plasmids. OBJECTIVE:To study the biological features of bone marrow derived stromal cells from transgenic rats with green fluorescent protein gene. METHODS:Bone marrows were isolated from the bilateral long bones of 2-week-old transgenic rats with green fluorescent protein gene, and cultivated in conditioned culture medium to obtain bone marrow mesenchymal stem cells with green fluorescent protein gene. The passage 5 bone marrow mesenchymal stem cells with green fluorescent protein gene were analyzed by flow cytometry to detect cellsurface molecules, and were induced in calcium or adipogenic induction medium. After calcium induction, the alizarin red staining was performed to test the formation of calcium concentration. After adipogenic induction, bone marrow mesenchymal stem cells were detected with oil red O staining. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cells, stably expressing green fluorescent protein gene, were obtained successful y from the transgenic rat with green fluorescent protein gene. The cells expressed CD90, CD105 strongly, but did not express or weakly expressed CD14 andCD45. After 3 weeks of calcium induction, jacinth calcium salts could be detected in the cells by alizarin red staining. At 3 weeks of adipogenic induction, the cells showed positive oil red O staining. The green fluorescent protein gene, as report gene, exerts no effect on the cellsurface molecules and multilineage potential of bone marrow mesenchymal stem cells, which can be used as an efficient tracer.

Command of the JCI standards and lessons learnt from practice and experience in JCI accreditation preparation,can help identify the gap between the hospitals and JCI standards.These standards can also guide the clinical pathway system,pharmacy intravenous admixture services system and medical business intelligence system.The information system built under such guidance not only makes an effective solution to existing problems,but also proves innovative.All these pave the way for successful JCI accreditation.

Objective To evaluate the academic level and the popularity of Chinese Journal of Orthopaedics from the point view of citation. Methods According to the information of Chinese Medical Citation Index (CMCI), the amount and distribution of the original articles in Chinese Journal of Orthopaedics cited by the journals included by CMCI were statistically analyzed. The data analysis included the percentage of cited articles, the number of citation of a single article, authors of most cited articles, geological regions of cited articles, citing journals, the self citing rate and years of citation. Results The percentage of cited papers (663 articles, 3728 times) in all published 1 182 articles in Chinese Journal of Orthopaedics from 2005 to 2009 was an average of 56.09%. The average time of original articles cited by other researchers was 5.62. The highest one was citated by 104 times. 210 articles (31.68% of total papers) were cited 5 or more times, and the all cited times from them were 2835 (76.04% of total cited times). 663 cited articles were written by 485 authors from different countries. The most frequently cited author wrote 12 papers. The number of authors with one paper cited was 386 (79.58% of total authors). The cited authors are from the mainland, HongKong, and Taiwan in China, as well as USA, Canada, France. Beijing, Shanghai and Tianjin are leading cities in orthopaedic research. Beijing was the first place (32.16% of total authors). There were 445 citing journals. There was 217 items self cited; the self- citing rate is 0.058. Conclusion Chinese Journal of Orthopaedics has provided high quality articles and has a strong influence in the field of medical research. It has been become an important resource for orthopaedic researchers and a vital medical journal in China.