OBJECTIVE:To provide reference for enhancing and standardizing the storage,dispensing and clinical use of the photophobic drug. METHODS:The management system and directory of photophobic drugs were set up in our hospital,and photo-phobic drug were classified into drugs used away from light strictly and drugs used away from light appropriately. Special training for nursing staff and monthly inspection of photophobic drug storage and use by inspection group could help finding problems and doing timely rectification. The number of wards where the problems of clinical photophobic drug use were found were compared be-fore and after management. RESULTS & CONCLUSIONS:After 3-quarter management,the quantity of wards with problems de-creased from 69 to 2,decreasing by 97.1%. The management reinforce of photophobic drug can effectively promote the manage-ment of drugs to be standard and scientific and improve the safety and effectiveness of photophobic drug use.

The original meaning of Portfolio is briefcase, generally translated as study archives in our country, with learners as the center, through the process of goal setting, feedback learning, self-reflection, steps, to show yourself and target revision form of process evaluation with learners as the center, to continuously promote the personal comprehensive ability through reflective learning progress. Portfolio, introduced in clinical admissions bilingual teaching, through six aspects such as individual learning, classroom display, clinical teaching, practice process and result display and target changes, and through the learning process assessment (fuzzy comprehensive evaluation method) and professional knowledge assessment (mini) method evaluating the clinical practice, evaluates Portfolio, in order to improve the medical students' clinical thinking and their comprehensive ability of lifelong learning,and promote the medical students' all-round development.

Objective To establish a rat model of chronic atrophic gastritis and investigate the factors inducing gastric atrophy. Methods According to the repeated orthogonal design of L 8(2 7), 60% alcohol and 20 mmol/L sodium deoxycholate (served as factor A), 0.05%～0.1% ammonia water (factor B) and 0.05% indomethacin (factor C) were given, alone or in combination, to rats in three experiments for 3 months, 6 months and 9 months, respectively. Then the rats were sacrificed and pathologic changes of the gastric mucosa were studied by gross appearence and microscopy. Results Typical appearance of CAG, which could maintain over one month, were found in all rats treated with factor A,B,C alone or in combination for 6 or 9 months. Conclusion 60% alcohol, 20 mmol/L sodium deoxycholate, 0.05%～0.1% ammonia water and 0.05% indomethacin given to SD rats for 6 months could establish animal model of CAG with a 100% successful rate.

Molecular Probe Techniques ; Myocytes, Cardiac ; physiology ; Regeneration ; Stem Cells

Air Pollutants, Occupational ; analysis ; Carbon Tetrachloride ; analysis ; Chromatography, Gas ; Environmental Monitoring ; methods ; Workplace

Objective To compare the effects of different methods of honey sunburn on the contents of calycosin and formononetin in Hedysari Radix; To provide the basis for the establishment of the optimum processing technology. Methods By frying (traditional method), baking, and microwave methods put Hedysari Radix under honey sunburn. Agilent HC-C18 column (4.6 mm × 250 mm, 5 μm) was used; the mobile phase was acetonitrile-0.01% phosphoric acid solution, gradient elution (0–12 min, 30%–33% acetonitrile; 12–13 min, 31%–40% acetonitrile; 13–25 min, 40% acetonitrile) with velocity of 1.0 mL/min; detection wavelength was 248 nm;the column temperature was 30 ℃; sample volume was 10 μL. Results There was statistical significance in the contents of calycosin and formononetin of different methods of honey sunburn for Hedysari Radix. Among them, the contents of calycosin and formononetin in Hedysari Radix processed by honey roast were the highest, 7.9116 and 49.6996 μg/g, respectively; the contents of calycosin and formononetin in Hedysari Radix processed by traditional method were the lowest, 4.7767 and 37.2910 μg/g, respectively; the contents of calycosin and formononetin in raw Hedysari Radix and Hedysari Radix by honey microwave method were the same, 5.0802, 42.7989 μg/g, and 3.9839, 42.3145 μg/g, respectively. Conclusion Different honey sunburn methods for the contents of calycosin and formononetin in Hedysari Radix have certain effects, and honey roast method is the optimum method.

Objective To observe the affection of smoking on atherosclerosis. Methods The research objects were divided into two groups,smoking group and control group in 60 cases. intima-media thickness and the numbers of plaques were measured in both sides of common carotid arteries by gray-scale imaging.Results There was significant difference between smoking group and control group(P

Air Pollutants ; analysis ; Chromatography, High Pressure Liquid ; methods ; Paraquat ; analysis ; Workplace

Objective To study the design and fabricating method of orthosis for patients with leg length discrepancy. Methods Ischial weight bearing orthosis with prosthetic feet was made through efforts from weight,beauty and durabili-ty.The fabrication process included taking a negative plaster cast,modifying the positive model,forming,align-ment,and fitting the device to the patients. Results The orthosis was compensated for the patients'height with good appearance and convenience wearing. Conclusion Ischial weight bearing orthosis with prosthetic feet can help patients reconstruct walking function,therefore can be recommended.

Immobilized penicillin acylase was used for bioconversion of penicillin G into 6-APA in aqueous two-phase systems consisted of a light-sensitive polymer PNBC and a pH-sensitive polymer PADB.Partition coefficients of 6-APA was found to be:about 5.78,in the presence of 1% NaCl.Enzyme kinetic showed that reaction reached equilibrium at 7h or so.The 6-APA mole yields were 85.3%(pH 7.8,and 20 ℃) and this value was about 20%higher than control in reaction of single aqueous phase buffer.Partition coefficient of penicillin G(Na) washardly changeable,while partition coefficient of product,6-APA and phenylacetate acid was significantly changeable.Reason is due to Donnan effect of phase systems andhydrophobicity of products.The change of partition coefficients of products also affects bioconversion yield of products.In the aqueous two-phase systems,substrate,penicillin G,products 6-APA and phenylacetate acid are biased in top phase,while immobilized penicillin acylase is completely partitioned in bottom.Substrate,penicillin G enters into bottom phase,and it is catalyzed into 6-APA and phenylacetate acid,then the products enter into top phase.Finally,inhibition of substrate and products is removed to result in improvement of products yield.Moreover,immobilized enzymehashigher efficiency than immobilized cells and occupy smaller volume.Comparing with free enzyme,immobilized enzymehashigher stability,longer use life,completely partitioned in bottom phase and recycle.Bioconversion in two-phase systems using immobilized penicillin acylase showed outstanding advantage.The light-sensitive copolymer forming aqueous two-phase systems could be recovered by laser radiation at 488 nm or filtrated 450 nm light,while pH-sensitive polymer PADB could be recovered by isoelectric point(pH 4.1).The recovery of the two copolymers was 95%~99%.