Objective To explore the effect of dexmedetomidine preconditioning on antioxidant ability in Rats Brain with focal cerebral ischemia/reperfusion.Methods Forty-two healthy male SD rats,weighted 250-280 g,were randomly divided into three groups (n = 14):sham-operation group (group Sham):in which carotid artery was exposed but MCAO was not performed;ischemia/reper-fusion group(group IR):NS were injected intraperitoneally at 30 minutes before the MCAO;dexme-detomidine group (group Dex):dexmedetomidine 100 μg/kg were injected intraperitioneally injected at 30 minutes before the MCAO.Focal cerebral ischemia-reperfusion (IR)model in rats was made by transient occlusion of the middle cerebral artery occlusion (MCAO)using a nylon thread with rounded tip inserted into internal carotid artery and advanced cranially until resistance was met.MCAO was maintained for 2 hours followed by 24 hours reperfusion.Neurologic deficit scores(NDS),the infarc-tion volume as well as the activities of endogenous antioxidants (such as superoxide dismutase(SOD), glutathione peroxidase(GSH-PX),glutathione reductase (GR),catalase (CAT))in ischemic brain were evaluated 24 h after reperfusion.Results Compared to group Sham,the neurologic deficit scores and the infarction volume in group Dex and IR were significantly higher,but the concentration of SOD,GSH-PX,GR,CAT were significantly lower(P <0.05).Compared to group IR,however,the neurologic deficit scores and the infarction volume in group Dex were significantly lower,but the concentra-tion of SOD,GSH-PX,GR,CAT were significantly higher (P <0.05).Conclusion Dexmedetomidine can protect the brain from focal cerebral ischemia-reperfusion injury,and its mechanism maybe relate to preserving the activities of endogenous antioxidants.

Objective:To carry out the service in the oncology by medical volunteers, provide more comprehen-sive and intimate quality nursing services guidance with nursing staff together for cancer patients. Methods:Making the medical volunteers service application system, service process and service standards. According to the patients to nursing job satisfaction questionaire by a third party, through telephone follow-up forms, carry out the satisfac-tion survey. Combined with their own advantages of TCM medical volunteers, to carry out the diet instruction, psy-chological counseling, massage, warm room service, telephone return services such as ward, score of life quality of tumor patients on admission and after the volunteer service. Results:Ward accompanying rate dropping, increasing patient satisfaction with nursing service, improving the degree of identification of volunteer service, tumor patients and improving the quality of life, and is conducive to building a perfect medical students social practice platform. Conclusion:Volunteer services improve the quality of nursing service, cultivating a correct outlook on life, values and morality of medical volunteers, strengthening medical volunteer service consciousness, achieving win-win for patients, departments, volunteers.

Objective To evaluate the effect of comprehensive intervention measures on reducing the bacterial colonization of the intensive care unit (ICU)bedside notebook computer keyboards and mice.Methods According to bacterial contamination of ICU bedside notebook computer keyboards and mice,comprehensive intervention meas-ures were taken (including wiping computer keyboards and mice with alcohol twice a day,intensifying hand hy-giene,and monitoring hand-washing compliance),specimens of keyboards and mice were taken,bacterial detection results were analyzed.Results Before intervention,bacterial counts of keyboards and mice of 8 specimens were all>5 CFU/cm2 ,detection rate of Bacillus subtilis was 64.71 %,Micrococcus ,coagulase negative staphylococcus, Acinetobacter baumannii ,and Klebsiella pneumoniae were 17.65%-58.82%,Escherichia coli and Acinetobacter calcoaceticus were both 5.88%.After comprehensive intervention measures were taken,bacterial counts of only two specimens of keyboards and mice were >5 CFU/cm2 ,the qualified rate of monitoring result after intervention was significantly higher than before intervention (88.24% vs 52.94%;χ2 =5.10,P <0.05).Conclusion Compre-hensive intervention measures can significantly reduce bacterial count on ICU bedside notebook computer keyboards and mice,and decrease the occurrence of healthcare-associated infection.

Intracranial arterial stenosis is the most common vascular lesions in patients with acute stroke in Chinese population,and most of them are middle cerebral artery (MCA) stenosis.The treatment of asymptomatic MCA stenosis is mainly to control the risk factors and treat with drugs,and the prognosis is better.Statins can reduce new stroke events.The treatment programs of symptomatic MCA stenosis are mainly to control the risk factors,treat with drugs and stenting.Now it is considered that anticoagulants are not superior to antiplatelet drugs in the treatment of MCA stenosis,but whether dual antiplatelet drugs are superior to monotherapy and stenting remains to be determined.The prognosis of symptomatic MCA stenosis is poor.The prognosis of acute phase mainly depends upon imaging features,and the risk of recurrent stroke is primarily associated with the MCA stenosis.Future research should focus on whether dual antiplatelet therapy is superior to monotherapy,and whether stenting is superior to drug treatment,as well as the relationship between the degree of MCA stenosis and prognosis of acute phase or the risk of recurrent stroke.

Objective To observe the effects of enzymolysis of human B like antigen by ? galactosidase treatment on the morphology and function of cynomolgus monkey red blood cell (RBC) and to evaluate the safety of the enzyme treated RBC in animal transfusion. Methods RBC with human B like antigen was treated by recombined ? galactosidase and the effect was evaluated by absorption elution test. Meanwhile, morphology and function of the treated RBC were examined and compared with pre treatment RBC. ? galactosidase treated RBC was labeled with FITC and then infused to blood group A cynomolgus monkeys. Flow cytometry was used to detect the survival of donor RBC in recipient’s blood. Blood chemistry and urinalysis of recipients were performed before and after transfusion. Results The human B like antigen of cynomolgus monkey RBC was obliterated by ? galactosidase treatment and the treated RBC maintained normal morphology and function. Survival at 24 h after transfusion was 84.6% and 68.1%. T 1/2 were 7d and 8d versus 13d in the controls. There were no change in blood chemistry and urinalysis of the recipients. Conclusion Enzymolysis of cynomolgus monkey RBC does not affect the cellular function and morphology. Transfusion of the enzyme treated human B like RBC into human A like cynomolgus monkeys is safe.

BACKGROUND: Serum uric acid level is associated with some chronic diseases and prognosis of severe infection. This study aimed to investigate the relationship between serum uric acid (SUA) and prognosis of infection in critically ill patients. METHODS: The data from 471 patients with infection admitted from January 2003 to April 2010 were analyzed retrospectively at Huashan Hospital Affiliated to Fudan University, Shanghai, China. The data of SUA, serum creatinine, blood urea nitrogen (BUN) and other relevant examinations within 24 hours after admission were recorded and the levels of SUA in those patients were described, then Student's t test was used to evaluate the relationship between SUA and pre-existing disorders. Different levels of SUA were graded for further analysis. The Chi-square test was used to examine the difference in the prognosis of infection. RESULTS: The mean initial level of SUA within 24 hours after admission was 0.232±0.131 mmol/L and the median was 0.199 mmol/L. Remarkable variations in the initial levels of SUA were observed in patients with pre-existing hypertension (t=–3.084,P=0.002), diabetes mellitus (t=–2.487, P=0.013), cerebral infarction (t=–3.061,P=0.002), renal insufficiency (t=–4.547,P<0.001), central nervous system infection (t=5.096,P<0.001) and trauma (t=2.875,P=0.004). SUA was linearly correlated with serum creatinine and BUN (F=159.470 and 165.059, respectively,P<0.001). No statistical correlation was found between the initial levels of SUA and prognosis of infection (χ2=60.892,P=0.100). CONCLUSION: The current study found no direct correlation between the initial levels of SUA after admission and prognosis of infection in critically ill patients.

This paper analyzes the reasons for quarantined patients' incomplete obedience to medical orders and comes up with relevant countermeasures as follows.1.External compulsory medical treatment should be combined with patients' internal self-consciousness.2.It is necessary to strengthen the mutual communication between doctors and quarantined patients,and observation for patients' condition.3.It is necessary to strengthen mental care and respect for patients' legal rights.4.A positive role should be taken in improving treating environment and give mental support from family members.

The structure of social network and its information spreading characteristics in 41 users of Sina microb-logs were studied , which showed that the exchange level was quite low in the 41 users of health information in Sina microblogs, usually in small groups.Although the 41 users were found in the core area, their exchange was sporadic even with no exchange in the core nodes or in other nodes, thus effective information sharing could not be achieved and information could not be widely spread .