Glycine/analogs & derivatives* ; Humans ; Vasculitis, Leukocytoclastic, Cutaneous

<b>Objective:b> To investigate the rates on prevalence, awareness, treatment and control of hypertension in population older than 15 years of age in Beijing, 2013-2014. <b>Methods:b> A cross-sectional survey was conducted in Beijing between 2013-2014. Stratified multistage random sampling method was used to select representative sample of 13 057 Chinese individuals aged over 15 years, from the general population. Blood pressure was measured for three readings at sitting position after resting for at least five minutes with an average reading recorded. A standardized structured questionnaire was developed to collect history of hypertension and antihypertensive treatment. <b>Results:b> A total of 4 663 community residents aged over 15 years were hypertensive among the 13 057 individuals, with the standardized prevalence rate as 32.7%, in Beijing area. The age-standardized prevalence rates of hypertension appeared 34.6% in men and 30.8% in women. The age-and sexstandardized prevalence of hypertension rates were 33.3% in urban and 24.6% in rural areas. The prevalence of hypertension increased with age and appeared higher in men than in women, in urban than in rural residents. Among the hypertensive patients, rates of awareness, treatment and control were 66.8%, 64.6% and 31.6%, respectively. <b>Conclusion:b> High prevalence of hypertension with low rates on awareness and treatment and control, appeared in the general population of Beijing. Related strategies should be developed regarding prevention, control and management of hypertension, to reduce the burden of this disease.
Adolescent ; Adult ; Age Distribution ; Aged ; Antihypertensive Agents/therapeutic use* ; Asian People/statistics & numerical data* ; Awareness ; Blood Pressure ; Blood Pressure Determination ; China/epidemiology* ; Cross-Sectional Studies ; Female ; Health Knowledge, Attitudes, Practice ; Humans ; Hypertension/epidemiology* ; Male ; Middle Aged ; Prevalence ; Rural Population ; Sex Distribution ; Surveys and Questionnaires ; Urban Population ; Young Adult

［摘要］ 目的：探讨miR-183 对脑胶质瘤细胞放射敏感性的影响。方法：2020 年10 月至2021 年6 月，收集秦皇岛市第一 医院40 例脑胶质瘤组织标本，对T98G 细胞进行梯度剂量X 射线（0、2、4、6 Gy）照射。采用qPCR 检测miR-183、细胞质多腺 苷酸化元件结合蛋白1（cytoplasmic polyadenylation element-binding protein 1，CPEB1）在脑胶质瘤组织、T98G 细胞和经X 射线 照射的T98G 细胞中的表达量。将miR-183 inhibitor 转染T98G 细胞后下调miR-183 表达，经6 Gy X 射线垂直照射，CCK-8 法、 流式细胞术和WB 法，分别检测T98G 细胞增殖能力、细胞凋亡率及BAX 和Bcl2 蛋白表达量。Targetscan 软件预测和双荧光 素酶报告基因实验检测miR-183 与CPEB1 的靶向关系。下调CPEB1 表达后，经6 Gy X 射线照射，分别用CCK-8 法、流式细胞 术和WB 法检测T98G 细胞增殖能力、细胞凋亡率及BAX 和Bcl2 蛋白表达量。将pcDNA-CPEB1 或CPEB1 siRNA 质粒转染 T98G细胞，分别下调或过表达CPEB1 后，检测miR-183 通过CPEB1 对T98G细胞放射敏感性的影响。结果：脑胶质瘤组织和 细胞中miR-183 呈高表达，CPEB1 mRNA 呈低表达。T98G 细胞中miR-183 的表达量随着X 射线放射剂量增加而降低 （P<0.05），CPEB1 表达量随着X 射线放射剂量增加而升高（P<0.05）。6 Gy X 射线照射T98G 细胞后，下调miR-183 可降低细 胞增殖能力、增加细胞凋亡率，而过表达miR-183 则起到相反作用（P<0.05）。miR-183 靶向CPEB1 mRNA 且负调控CPEB1 表 达。下调CPEB1 表达后，经6 Gy X 射线照射可显著提高T98G 细胞增殖能力（P<0.05）、降低细胞凋亡率（P<0.05），miR-183 可 逆转CPEB1 过表达对细胞T98G 放射敏感性的促进作用（P<0.05）。结论：下调miR-183 的表达能够负调控CPEB1，从而增强 脑胶质瘤细胞的放射敏感性。

[Abstract] Objective: To observe the effect of allogeneic platelets transfusion on the invasion and metastasis of human lung cancer A549 cells, and to preliminarily explore its mechanism of action. Methods: Eighty-nine patients with advanced lung cancer, who had received platelet transfusion in the Chemotherapy Department of Fourth Hospital of Hebei Medical University between January 2017 and December 2018, were enrolled in this study. The study cells were randomized into Ctrl group (A549 cells co-incubated with culture medium), Before group, and After group (A549 cells co-incubated with plasma Before and After platelet transfusion, respectively). The migration and invasion of A549 cells co-cultured with plasma before and after platelet transfection were detected by Scratch and Transwell experiments. The expression of MMPs, TIMPs and epithelial-mesenchymal transition (EMT) related proteins E-cadherin, N-cadherin and Vimentin, as well as vascular endothelial growth factor (VEGF) and its receptor 2 (VEGFR2) were detected by Western blotting (WB) method. Results: The scratch healing ability of A549 cells in After group was significantly higher than that of Ctrl group and Before group [(73.67±2.60)% vs (58.33±2.33)%, (35.33±2.03) %; P<0.01, vs Ctrl group; P<0.05, vs Before group], and there was also a significant difference between Before group and Ctrl group (P<0.05). The results of cell migration experiment showed that the number of transmembrane cells in After group was significantly higher than that in Ctrl group and Before group [(69.67±7.84) vs (18±2.08) and (39.33±2.03), all P<0.01]. The cell invasion experiment showed that the number of transmembrane cells in After group was significantly higher than that in Ctrl group and Before group [(59.34±3.46) vs (18.34±1.56) and (37.58±2.79), all P<0.01]. When A549 cells were co-incubated with plasma before and after platelet transfusion for 48 h, it was found that the expressions of MMP9 and MMP2 were increased (P<0.05), while their inhibitors TIMP1 and TIMP2 were decreased (P<0.01); the expressions of EMT-related proteins N-cadherin and Vimentin were increased (P<0.05), but E-cadherin was decreased (P<0.01); the expressions of angiogenesis related proteins VEGF and VEGFR2 were increased (P<0.05). Conclusion: Alloplatelets transfusion can promote the invasion and metastasis of lung cancer A549 cells, which may be realized by regulation of the expressions of EMT, metallomatrix protease and vascular growth factor-related proteins.

<b>Objectiveb> To observe the effect of Huoxin pill （concentrated pill） combined with Baduanjin on the prognosis of patients with acute myocardial infarction complicated by percutaneous coronary intervention （PCI）. <b>Methodsb> 120 patients with acute myocardial infarction complicated with heart failure who received emergency interventional treatment in our hospital from January 2022 to January 2023 were randomly divided into western medicine treatment group and Traditional Chinese Medicine （TCM）comprehensive treatment group. Western medicine treatment: standard western medicine treatment + five prescriptions for cardiac rehabilitation; based on western medicine treatment, Huoxin pill （concentrated pill） combined with Baduanjin therapy was added to the TCM comprehensive treatment group, and the follow-up was 6 months. The observed indexes were exertion angina pectoris scale, Chinese Medicine Syndrome Scale, Chinese medicine syndrome treatment effect evaluation, 6-minute walking test （6MWT）, left ventricular ejection fraction （LVEF）, and brain natriuretic peptide precursor （pro-BNP）. <b>Resultsb> Sixty patients were enrolled in the two groups respectively. During the treatment, 2 cases fell off in the western medicine treatment group and 8 cases fell off in the TCM comprehensive treatment group, and a total of 110 cases were enrolled in the group. Compared with the western treatment group, TCM combined therapy significantly improved angina pectoris scale score, TCM Syndrome Scale score, pro-BNP, LVEF and 6MWT （P<0.001）. There were no significant differences in blood routine, liver and kidney function, potassium, blood glucose, blood lipids and cardiac Troponin I （cTnI） between the two groups （P>0.05）. No adverse cardiovascular events occurred during the entire treatment period in both groups. <b>Conclusionb> Huoxin pill （concentrated pill） combined with Baduanjin was more effective than western therapy in improving LVEF, 6MWT distance, exercise tolerance and cardiac function in patients with acute myocardial infarction complicated with heart failure.

[摘 要] 目的：探讨过氧化物酶体膜蛋白4（PXMP4）对肝细胞癌（HCC）细胞迁移和侵袭及上皮间质转化（EMT）进程的影响。方法:采用生物信息学和免疫组织化学方法分析HCC组织中PXMP4的表达，分析其与患者临床病理特征的相关性。在HCCLM3和MHCC97H细胞中干扰PXMP4，在Huh7和MHCC97L细胞中过表达PXMP4，利用WB和qPCR法验证干扰/过表达效率。利用CCK-8、划痕愈合实验和Transwell侵袭实验检测干扰/过表达PXMP4对HCC细胞增殖、迁移和侵袭能力的影响，采用WB法检测干扰/过表达PXMP4或0、5、10和20 μmol/L U0126处理对HCC细胞中N-cadherin、E-cadherin、vimentin、细胞外信号调节激酶（ERK）、p-ERK表达的影响。结果：生物信息学和免疫组织化学分析显示，HCC组织中PXMP4呈高表达（P<0.05）。临床病理分析发现，PXMP4的表达与肿瘤分化程度有关联（P<0.05）。在HCC细胞中，干扰PXMP4后抑制细胞增殖、侵袭和EMT进程（P<0.05）；反之，过表达PXMP4后促进HCC细胞增殖、侵袭及EMT进程（P<0.05）。此外，PXMP4通过激活ERK1/2信号通路促进HCC细胞EMT进程，U0126处理同样能够抑制HCC细胞EMT进程。结论：PXMP4在HCC组织中呈高表达且与HCC细胞分化有关联，PXMP4通过激活ERK1/2信号通路促进HCC细胞EMT进程。

Background & Objective: No clinical study of any interferon beta therapy has yet been successfully conducted in Chinese multiple sclerosis patients, probably due to the low incidence of this disease in China. The primary objective of this study was to demonstrate that treating multiple sclerosis patients of Chinese origin with interferon beta-1b has a beneficial effect on disease course, as measured by the decrease of newly active lesions on magnetic resonance imaging. Methods: Chinese patients diagnosed with relapsing-remitting or secondary-progressive multiple sclerosis were enrolled in this multicenter, open label, single-arm study. Following a 3-month pre-treatment phase, patients were treated with 250 µg interferon beta-1b subcutaneously every other day for 6 months. Patients had regular assessments for treatment safety and efficacy of the treatment. Results: Thirty seven patients completed the trial. Significant decreases in the number of newly active lesions were observed in the 6-month treatment period compared with the pre-treatment period (median decrease 1.5 lesions, p<0.001). Most adverse events were mild and transient and no serious ones were observed. Conclusions: Treatment with interferon beta-1b significantly reduced the occurrence of new lesions and was well tolerated in this Chinese population. These findings support the use of interferon beta- 1b for treating Chinese MS patients.

Adolescent ; Female ; Humans ; Sacrum ; pathology ; surgery ; Spondylolysis ; diagnosis ; pathology ; surgery

<b>Objective:b> To investigate the infection pattern and etiological characteristics of a case of human infection with highly pathogenic avian influenza A (H7N9) virus and provide evidence for the prevention and control of human infection with highly pathogenic avian influenza virus. <b>Methods:b> Epidemiological investigation was conducted to explore the case's exposure history, infection route and disease progression. Samples collected from the patient, environments and poultry were tested by using real time reverse transcriptase-polymerase chain reaction (RT-PCR). Virus isolation, genome sequencing and phylogenetic analysis were conducted for positive samples. <b>Results:b> The case had no live poultry contact history, but had a history of pulled chicken processing without taking protection measure in an unventilated kitchen before the onset. Samples collected from the patient's lower respiratory tract, the remaining frozen chicken meat and the live poultry market were all influenza A (H7N9) virus positive. The isolated viruses from these positive samples were highly homogenous. An insertion which lead to the addition of multiple basic amino acid residues (PEVPKRKRTAR/GL) was found at the HA cleavage site, suggesting that this virus might be highly pathogenic. <b>Conclusions:b> Live poultry processing without protection measure is an important infection mode of "poultry to human" transmission of avian influenza viruses. Due to the limitation of protection measures in live poultry markets in Guangzhou, it is necessary to promote the standardized large scale poultry farming, the complete restriction of live poultry sales and centralized poultry slaughtering as well as ice fresh sale.
Animals ; Chickens ; China ; Commerce ; Humans ; Influenza A Virus, H7N9 Subtype/pathogenicity* ; Influenza in Birds/virology* ; Influenza, Human/virology* ; Phylogeny ; Poultry/virology* ; Real-Time Polymerase Chain Reaction ; Zoonoses