OBJECTIVE: To verify the effect of Matrigel, a ECM complex from Engelbreth-Holm-Swarm(EHS) mouse sarcoma on the preimplantation development and apoptosis of mouse fertilized eggs. METHOD: Late Pronucleus stage eggs were cultured through the blastocyst stage in the presence of Matrigel (0.5%, v/v). Characteristics of apoptosis and cell number assessed by Hoecst staining and TUNEL labeling at the blastocyst stage, respectively. RESULTS: Morphological development, number of cells per embryo was significantly increased but rate and number of TUNEL positive nuclei of the embryo were decreased in the presence of Matrigel. CONCLUSION: This result suggested that at low concentration of Matrigel improves both viability and morphological development in the preimplantation mouse embryos.
Animals ; Apoptosis* ; Blastocyst ; Cell Count ; Eggs ; Embryonic Structures* ; In Situ Nick-End Labeling ; Mice* ; Ovum ; Sarcoma ; Zygote

Bile* ; Choledochal Cyst* ; Humans ; Infant* ; Peritonitis*

A total of 72 cases of internal biliary drainage procedure has been done percutaneously at Departement ofRadilogy, Seoul Natinal Universtiy Hospital for recent 4 and half years since August 1981. Five different types ofinternal drainage including endoprosthesis were applied to various conditions with different obstruction levels.The different method of procedure in each type of internal drainage was described and the results were analysed.1. Among the clinical diagnosis in 72 cases, carcinoma of bile duct was in 37 cases as the most frequent one. Thenext was pancreas head carcinoma in 16 cases, followed by metastatic carcinoma and other disease. 2. Type Iinternal drainage, classical internal and external type with distal end in duodenum, was applied in 43 cases asthe most comon one. Type II, modification of type I with distal end in CBD, was applied in 17 cases. Type III,endoprosthesis distal end into duodenum, was applied in 7 cases. Type IV, endoprosthesis just across theobstruction in bile duct, was applied in 2 cases, Type V, combined type of any internal drainage with externaldrainage, was applied in 3 cases. 3. According to various obstruction level, prefered type could be selected. Forproximal obstruction type II and IV were preferred and for distal obstruction type III was chosen. However, type Icould be applied in any situation. 4. Early complication occurred in 9 cases(12.5%). Clinical imporvement withdecrease in bilirubin level was observed 17 of 21 cases in which follow-up data was available over 2 months. 5.Though the experience with those 72 cases it is recommended that appropriate type of internal biliary drainageshould be selected according to prognosis, obstruction level and clinical findings of each patient.
Bile Ducts ; Bilirubin ; Diagnosis ; Drainage ; Duodenum ; Follow-Up Studies ; Head ; Humans ; Methods ; Pancreas ; Prognosis ; Seoul

A fracture of the affected extremity in patients with Klippel–Trenaunay–Weber syndrome can be fatal due to massive bleeding and show poor results. A 42-year-old male presented with an old fracture of the right femoral shaft with metal failure. We planned an operation to remove the previously fixed plate and to perform re-fixation using an intra-medullary nail. Preoperative angiography was performed and the arteriovenous malformations were embolized in order to reduce the risk of bleeding. After angiography, the previously fixed plate was removed.After the operation, a second angiography was performed immediately and the venous malformation was embolized. One week after the first operation, a second operation was performed in order to reduce the fracture and to perform re-fixation using an intramedullary nail. The patient is being followed without major complication over a period of seven years after surgery. We recommend careful planning of preoperative and postoperative angiography and embolization in order to reduce the risk of bleeding in patients with Klippel–Trenaunay–Weber syndrome.

AIDS, which is being spreaded world-wide, would have the number of the patients and the infected persons to several million persons since the first patient had been reported among the homosexualists in 1981, and in our country patients of 323 persons had been found till 1993, therfore we could have significant concern and warning. Accordingly, this writer intended to present basic data to the blood Project and AIDS preventive movement by investigating knowledge, attitude and concern regarding AIDS including blood donors, and analyzed data of 1,920 pages by questionaire method from March to August 1994. According to the results, it is rather well-known as far as general knowledge, attitude and concern regarding AIDS are concerned, however, items which are 20-40% wrongly known are as followings: AIDS is infected by virus. It is infected by insects such as mosquito. It is infected with tight kiss with the person infected with AIDS. Risk of being infected with AIDS will be reduced if using condom when sexual intercourse. It may be infected through the foods. It may be infected by the same bacteria attacking venereal disease. And WHO(world Health Organization) designated Dec. 1st as AIDS'Day. Items which are 40-60 wrongly known are as followings: AIDS can be infected by blood donation, Now vaccine preventing AIDS has been developed. In our country, Preventive law of AIDS is established. And in the field of the attitude and concern, it was surveyed as important as being hahdied by the broadcasting and newspaper, and 60470% of the persons answered that they would avoid AIDS infected person, and 76% of male and 87.1% of female gave the reply thet AIDS infected persons should be administered extraordinarily by isolated accommodation by the nation.
Bacteria ; Blood Donors* ; Coitus ; Condoms ; Culicidae ; Female ; Humans ; Insects ; Jurisprudence ; Male ; Periodicals ; Sexually Transmitted Diseases ; World Health Organization

Histopathological diagnosis of brain stem glioma should be performed for the purpose of the determination of its management and clinical course, but its surgical biopsy has been followed by high mortality and morbidity. We performed the tissue sampling for histological examination with BRW stereotaxic system under local anesthesia successfully.
Anesthesia, Local ; Biopsy* ; Brain Stem* ; Brain* ; Diagnosis ; Glioma* ; Mortality

No abstract available.
Lymph Nodes* ; Uterine Cervical Neoplasms*

No abstract available.
Colony-Stimulating Factors* ; Transplantation*

No abstract available.

No abstract available.
Humans ; Orthognathic Surgery*