No abstract available.
Korea* ; Orientia tsutsugamushi* ; Rickettsia*

No abstract available.
Alleles* ; Base Sequence* ; DNA* ; HLA-DR4 Antigen* ; HLA-DRB1 Chains*

No abstract available.
HLA-DR Antigens* ; Polymerase Chain Reaction*

No abstract available.
HLA-DR Antigens* ; Polymerase Chain Reaction* ; Spermatozoa*

14-3-3 proteins are cytoplasmic proteins of about 29 kDa and have a minimum of seven isoforms. This protein is important in signal transduction with the ability of binding with phosphoserine of many signalling proteins. We expressed 14-3-3 protein tagged with 6 histidine residues in E. coli and purified the protein by nickel affinity chromatography. Using this purified protein as an antigen, we made rabbit antisera and mouse monoclonal antibodies to 14-3-3 zeta isoform. We subcloned cDNA of 14-3-3 zeta isoform derived from HeLa cell lamda gt 11 library into an E. coli expression vector which is designed to express heterologous protein with N- terminal 6 hidtidine tag. BALB/c mice were immunized with purified 14-3-3 protein and the hybridoma clones which produce monoclonal antibodies angainst 14-3-3 protein were selected. These monoclonal antibodies reacted with the recombinant protein expressed in E. coli as well as the 29-kDa native protein in various cell lines. However, they did not immunoprecipitate 14-3-3 protein. The monoclonal antibodies produced in this study can be valuable tools for the identification of the 14-3-3 in signal transduction study.
14-3-3 Proteins ; Animals ; Antibodies, Monoclonal ; Cell Line ; Chromatography, Affinity ; Clone Cells ; Cytoplasm ; DNA, Complementary ; Escherichia coli* ; Escherichia* ; HeLa Cells ; Histidine ; Humans* ; Hybridomas ; Immune Sera ; Mice ; Nickel ; Phosphoserine ; Protein Isoforms ; Signal Transduction ; Staphylococcal Protein A

It has been emphasized that the treatment of choice for the trochantric fracture of the femur is open reduction and rigid internal fixation to reduce complications by early ambulation. The incidence of trochanteric fracture of the femur in the young age group has been considerably increased in recent years because of increased traffic and industrial accidents. The author treated 38 cases of trochanteric fracture of the femur in the year 1974 through 1979, at the Department of Orthopaedic Surgery, School of Medicine, Soon Chun Hyang College. The results were as follows: 1. In sex distribution, 24 out of 38 were males and 14 were females. 16 cases out of 24 male patients were in the age group 20-40 and 9 cases out of 14 female were over 60 years of age. Over all mortality was 7.9%. 2. The numbers of patients of type I and III were 11 cases in each type out of 38 trochanteric fracture. 3. 26 cases out of 38 cases were treated by open reduction and internal fixation and the others were by traction and cast. 4. The applied metal devices were 3 types: Smith-Peterson nail and Thornton or McLaughlin plate, compression hip screw, and multiple pinning. 5. Mean duration of bony union is shorter in the group of open reduction and internal fixation (13 weeks) than the group of traction and cast (15 weeks). The cause of difference is that the 6 out of 9 cases of type IV and V were included in conservative group. 6. The incidece of the complications such as coxa vara, slipping screw, long nail and traction palsy occurred higher in the group fixed with Smith-Peterson nail and plate than the group fixed with compression hip screw.
Accidents, Occupational ; Coxa Vara ; Early Ambulation ; Female ; Femur ; Hip ; Humans ; Incidence ; Male ; Mortality ; Paralysis ; Sex Distribution ; Traction

No abstract available.
Orientia tsutsugamushi* ; Rickettsia* ; Ulsan*

BACKGROUND: Although exact mechanism of adult respiratory distress syndorme (ARDS) is not known, many evidences suggest that inflammatory mediators such as protease and oxygen radicals may be the final common pathway to the endothelial injury. It is gradually accepted that activated neutrophils play a major role in the process. Pentoxifylline is a methylated xanthine with a wide range of actions that make it theoretically useful in the treatment of ARDS. Studies show that it decreases neutrophil phagocytosis and superoxied production. The aim of this experiment is to determine whether pentoxifylline has a preventive effect on oleic acid-induced lung injury. METHODS: Oleic acid (0.08 ml/kg) was infused in 10 mongrel dogs in order to induce acute lung injury. Pentoxifylline (20 mg/kg) was administered 40 mins before oleic acid injection. The author made a comparative studies on the effects of pentoxifylline on hemodynamics and gas exchange before oleic acid injection and at 15, 30, 45, 60, 90 mins after oleic acid injection. RESULTS: There were not significant changes in mean arterial pressure, cardiac output, systemic vascular resistence, pulmonary wedge pressure intrapulmonary shunt in both groups. There were statistical significance in arterial oxygen tension, pulmonary artery pressure, pulmonary vascular resistance between both groups (p<0.05). CONCLUSIONS: Pentoxifylline is a noteworthy drug that could be a candidate as a therapy to help prevent effect in lung injuries that share a common mechanism with oleic acid-induced lung injury.
Acute Lung Injury ; Adult ; Animals ; Arterial Pressure ; Cardiac Output ; Dogs ; Hemodynamics ; Humans ; Lung Injury* ; Lung* ; Neutrophils ; Oleic Acid ; Oxygen ; Pentoxifylline* ; Phagocytosis ; Pulmonary Artery ; Pulmonary Wedge Pressure ; Reactive Oxygen Species ; Vascular Resistance ; Xanthine

Orientia tsutsugamushi, the causative agent of scrub typhus, is an obligate intracellular parasite. Previously we have shown that it persistently infect the human endothelial cell line ECV304. In this study, we have investigated the mechanism of in vitro persistent infection, which could be maintained for over a year without addition of normal cells. The persistently infected cultures exhibited cyclic changes in the host cell number, which resulted in a net increase of cell number. Floating cells detached from the culture plate bottom were filled with rickettsial cells and lost normal morphology. Only part of attached EVC304 cells was infected with O. tsutsugamushi. Some of ECV304 cells that still attached to the bottom were free of rickettsial cells. Rickettsial cells in the floating cells could not effectively infect new EVC304 cells while those in the attached cell could infect new cells with high efficiency. Host cells that allowed vigorous rickettsial multiplication and resultantly lost viability, and low of infectivity of the bacteria in the dead cells might have allowed in vitro persistent infection of O. tsutsugamushi.
Apoptosis ; Bacteria ; Cell Count ; Endothelial Cells ; Humans ; Orientia tsutsugamushi* ; Parasites ; Scrub Typhus

Combined anesthesia has been associated with less sedation, earlier ambulation, higher pulmonary flow rates, improved oxygenation, blunting of stress response and better pain control in the postoperative period than general anesthesia. Total intravenous anesthesia has many advantages compared with inhalation anesthesia, but also has several disadvantages such as hypertension, inappropriate anesthetic, delayed recovery and emergence delirium For improvement of this problems, the authors tried epidural anesthesia combined with continuous intravenous infusion of propofol which is a short acting intravenous anesthetic and has characteristics of rapid and clear-headed recovery. Fifty-three patients undergoing elective thoracic operation were randomly assigned to receive anesthesia with N2O-O2-enflurane (n=23), epidural anesthesia combined with N2O-O2-propofol infusion (3 mg/kg/hy; n=15), or epidural anesthesia combined with medical air-O2-propofol infusion (6 mg/kg/hy; n=15). We studied the hemodynamic changes and occurence of awareness and recovery time on those three groups. Although there were significant changes in the hemodynamics among the groups except CVP, but all values were within normal limit and there were no difference in the recovery time. We concluded that epidural anesthesia with medical air-O2-propofol infusion (6 mg/kg/hr) is acceptable altemative method for thoracic surgery.
Anesthesia ; Anesthesia, Epidural* ; Anesthesia, General ; Anesthesia, Inhalation ; Anesthesia, Intravenous ; Delirium ; Hemodynamics ; Humans ; Hypertension ; Infusions, Intravenous* ; Oxygen ; Postoperative Period ; Propofol* ; Thoracic Surgery* ; Walking