No abstract available.
Emergencies* ; Emergency Medicine* ; Humans

PURPOSE: The purpose of this study is to analyze the mode of recurrence and it's relating factors in gastric adenocarcinoma. MATERIALS AND METHODS: 1,446 patients who had been undergone gastrectomy for gastric adeno carcinoma from September 1983 to December 1996 (Department of Surgery, Korea University) were studied (median follow-up was 2.9 years). Of them, 243 patients who had been proven recurrence were studied on recurrence mode and clinicopathological factors. RESULTS: The mean duration to recurrence was 22 months. The modes of recurrence were locore gional (30.0%), peritoneal (23.0%), hematogenous (19.3%) and distant lymph node metastasis (4.1%) in order. In 23.5%, more than 2 recurrent pattern were combined. Age, gross type, tumor size and stage were statistically significant to the mode of recurrence. The disease free survival was calculated: regional lymph node, number of lymph node, stage, and tumor emboli had statistical significance in all types of recurrence and in locoregional recurrence, tumor size was significant. Multivariate analysis showed that regional lymph node influenced the disease free survival period in all cases and tumor size in locoregional recurrence. CONCLUSION: The number of positive regional lymph node and tumor size are important factors predicting the timing of recurrence after curative resection for gastric adenocarcinoma.
Adenocarcinoma* ; Disease-Free Survival ; Follow-Up Studies ; Gastrectomy ; Humans ; Korea ; Lymph Nodes ; Multivariate Analysis ; Neoplasm Metastasis ; Recurrence* ; Stomach Neoplasms

PURPOSE: Gastric cancer is the most common malignancy in Korea. Although treatment such as surgery, chemotherapy, and immunotherapy has greatly improved, the mortality rate of gastic cancer is still high, A new therapeutic trial is necessary to improve the cure rate of gastric cancer. Therefore we investigated the pre-clinical significance of HSV-tk gene therapy using retroviral vector for gastric cancer cell lines. MATERIALS AND METHODS: LNC/HSV-tk retroviral vector and PA317/LNC/HSV-tk producer cell line were constructed. HSV-tk gene transduction and expression were detected by PCR. An in vitro ganciclovir(GCV) sensitivity test was performed by MTT assay. To evaluate in vivo GCV sensitivity, GCV was intraperitoneally injected after tumor formation in the nude mice. Bystander effect was observed in vitro MTT assay using YCC- S-2 cell line and in vivo using N87 and YCC-S-2 cell lines. RESULTS: The in vitro GCV sensitivity test showed that the growth inhibition was 30~32% with 0.5 uM GCV and 52~77% with 500 uM GCV in the HSV-tk transduced cell line in comparison with 0- 5% with 0.5 and 500 uM GCV in the parent cell line. The in vivo GCV administration showed that the tumors induced by HSV-tk transduced N87 cell line and YCC-S-2 cell line decreased completely, while the tumors with the parent cell lines continued to grow in nude mice. We observed no tumor cells in tissue specimen of the tumor induced by the N87/HSV-tk cell line after. GCV administration. In vitro and in vivo bystander effects were observed in HSV-tk/GCV system due to the resultant cell death exceeding the proportion of HSV-tk transduced cells in the mixtures of HSV-tk transduced and parent cells. CONCLUSION: HSV-tk transduced gastric cancer cell lines showed sensitivity to GCV and a bystander effect was observed. These results suggested that HSV-tk/GCV system should be evaluated in the clinical settings.
Animals ; Bystander Effect ; Cell Death ; Cell Line* ; Drug Therapy ; Ganciclovir* ; Genetic Therapy ; Herpes Simplex* ; Humans ; Immunotherapy ; Korea ; Mice ; Mice, Nude ; Mortality ; Parents ; Polymerase Chain Reaction ; Stomach Neoplasms* ; Thymidine Kinase* ; Thymidine* ; Zidovudine

Granular cell tumors (GCT) are found in virtually any body site, including the tongue, skin, subcutaneous tissue, breast, rectum and vulva. However, they are rarely seen in the abdominal wall. We report here on a rare case of GCT in the rectus muscle of the abdominal wall. A 44-year-old woman presented with a non-tender, hard mass in the right lower abdominal wall. Upon microscopic examination, the tumor was found to comprise of large polygonal cells with an abundant eosinophilic granular cytoplasm and round to oval nuclei. Upon immunohistochemical staining, the large cells showed S-100 and CD68 positive granular aggregates in the cytoplasm. Many lysosomes of variable size were observed in the cytoplasm.
Abdominal Neoplasms/metabolism/*pathology ; Adult ; Female ; Granular Cell Tumor/metabolism/*pathology ; Humans ; Immunohistochemistry ; Rectus Abdominis/metabolism/*pathology ; S100 Proteins/metabolism

Granular cell tumors (GCT) are found in virtually any body site, including the tongue, skin, subcutaneous tissue, breast, rectum and vulva. However, they are rarely seen in the abdominal wall. We report here on a rare case of GCT in the rectus muscle of the abdominal wall. A 44-year-old woman presented with a non-tender, hard mass in the right lower abdominal wall. Upon microscopic examination, the tumor was found to comprise of large polygonal cells with an abundant eosinophilic granular cytoplasm and round to oval nuclei. Upon immunohistochemical staining, the large cells showed S-100 and CD68 positive granular aggregates in the cytoplasm. Many lysosomes of variable size were observed in the cytoplasm.
Abdominal Neoplasms/metabolism/*pathology ; Adult ; Female ; Granular Cell Tumor/metabolism/*pathology ; Humans ; Immunohistochemistry ; Rectus Abdominis/metabolism/*pathology ; S100 Proteins/metabolism

Tumor hypoxia contributes to the progression of a malignant phenotype and resistance to ionizing radiation and anticancer drug therapy. Many of these effects in hypoxic tumor cells are mediated by expression of specific set of genes whose relation to therapy resistance is poorly understood. In this study, we revealed that DNA-dependent protein kinase (DNA-PK), which plays a crucial role in DNA double strand break repair, would be involved in regulation of hypoxia inducible factor-1 (HIF-1). HIF-1beta-deficient cells showed constitutively reduced expression and DNA-binding activity of Ku, the regulatory subunit of DNA-PK. Under hypoxic condition, the expression and activity of DNA- PK were markedly induced with a concurrent increase in HIF-1alpha expression. Our result also demonstrated that DNA-PK could directly interact with HIF- and especially DNA-PKcs, the catalytic subunit of DNA-PK, could be involved in phosphorylation of HIF-1alpha, suggesting the possibility that the enhanced expression of DNA- PK under hypoxic condition might attribute to modulate HIF-1alpha stabilization. Thus, the correlated regulation of DNA-PK with HIF-1 could contribute to therapy resistance in hypoxic tumor cells, and it provides new evidence for developing therapeutic strategies enhancing the efficacy of cancer therapy in hypoxic tumor cells.
Antibodies/immunology ; Cell Hypoxia ; Cell Line, Tumor ; DNA Helicases/immunology/metabolism ; DNA-Binding Proteins/genetics/*metabolism/*physiology ; Deferoxamine/pharmacology ; Drug Resistance, Neoplasm/*physiology ; Humans ; Immunoprecipitation ; Neoplasms/enzymology/*metabolism ; Nuclear Proteins/genetics/*metabolism/physiology ; Phosphorylation ; Protein-Serine-Threonine Kinases/metabolism/*physiology ; Research Support, Non-U.S. Gov't ; Transcription Factors/genetics/*metabolism/physiology ; Up-Regulation

BACKGROUND/AIMS: We explored Korean physicians’ policies for surveillance of colorectal cancer (CRC) after curative surgery. METHODS: Web-based self-report questionnaires were developed. Invitations to participate were emailed to physicians who diagnosed and treated CRC from October 1 to November 15, 2015. The questionnaire consisted of the role doctors played in the surveillance, examination of surveillance, and duration of postoperative surveillance according to CRC stage or primary site of the cancer. RESULTS: Ninety-one physicians participated in the online survey, and 78 completed the survey. Sixty-seven participants (13%) answered “up to 5 years” for stage I surveillance duration; and 11 (13%) responded with a duration of > 5 years for stage I. A total of 61 (75%) responded with a surveillance duration of up to 5 years for stage II; and 19 (24%) responded with a duration of > 5 years for stage II. Sixty-seven (97%) and 61 (91%) physicians monitored patients with stage II/III every 3 or 6 months by laboratory examination and by abdominopelvic computed tomography scan for the first year, respectively. A total of 43 (53%) responded with a surveillance duration of up to 5 years for stage IV; and 46 (46%) responded with a duration of > 5 years for stage IV after curative resection. CONCLUSIONS: Korean physicians mostly followed up CRC using intensive postoperative surveillance. In preference to monitoring over a comparatively shorter period of time, the physicians tended to prefer monitoring patients post-operatively over a > 5 year period, particularly in cases of advanced-stage CRC.
Colorectal Neoplasms* ; Electronic Mail ; Follow-Up Studies ; Humans ; Surveys and Questionnaires

Multiple lymphomatous polyposis (MLP) is a term used to describe malignant lymphoma of the gastrointestinal tract that manifest as polyposis. MLP is believed to represent gastrointestinal involvement by mantle cell lymphoma (MCL), primarily based on its histologic and immunophenotypic similarities with MCL. We have experienced a case of MCL presented as a peculiar feature of multiple lymphomatous polyposis in a 49-year old male patient. He had been diagnosed diffuse large B cell lymphoma of nasopharynx and treated by radiation therapy and CHOP chemotherapy. He newly developed MCL after 10 years in complete remission of diffuse large B cell lymphoma. After newly developed lymphoma was compared with former diagnosed lymphoma, we concluded that they were not same type each other. We reviewed a total of 13 patients including this case, of MLP, which were reported in various Korean literature, and literatures are reviewed.
B-Lymphocytes* ; Drug Therapy ; Gastrointestinal Tract ; Humans ; Lymphoma* ; Lymphoma, B-Cell ; Lymphoma, Mantle-Cell ; Male ; Middle Aged ; Nasopharynx

Burkitt's lymphoma is a rare disease that belongs to the aggressive non-Hodgkin's lymphomas. Burkitt's lymphoma usually occurs in children or younger persons. There is a strong association between endemic Burkitt's lymphoma and Epstein-Barr virus, whereas the association is weaker in the sporadic form occurring in Western countries. Burkitt's lymphoma of the gastrointestinal tract occurring in adults has not been reported in Korea. We herein report an unusual case of Burkitt's lymphoma presenting with a palpable abdominal mass in a 52-year-old man infected with Epstein-Barr virus. A computed tomographic scan showed marked low density wall thickening of the terminal ileum. An endoscopy revealed a narrow lumen, shallow ulcerations, and irregular nodularity of the terminal ileum. Small bowel resection and hemicolectomy were performed and a histologic examination of the resected specimen confirmed the diagnosis. Six cycles of adjuvant chemotherapy were given and the patient has been doing well without evidence of recurrence.
Adult* ; Burkitt Lymphoma* ; Chemotherapy, Adjuvant ; Child ; Diagnosis ; Endoscopy ; Gastrointestinal Tract ; Herpesvirus 4, Human ; Humans ; Ileum ; Korea ; Lymphoma, Non-Hodgkin ; Middle Aged ; Rare Diseases ; Recurrence ; Ulcer

The Cronkhite-Canada Syndrome (CCS) is a monfamilial disorder of adults characterized by diffuse gastro-intestinal polyposis, ectodermal changes consisting of alopecia, dystrophy of nails and cutaneous hyper-pigmentation. The pathogenesis and the causes of CCS remain unknwon but the symptoms such as diarrhea and malnutrition are generally progressive, and the prognosis is knwon to be poor. Though the definitive treatment is not well known, it has been reported that the conservative management is the most important treatment, and that the clinical sourse can be reversible. One case was experienced involving Cronkhite-Canada Syndromen in a patient who was managed by conservative treatment and an other who was treated by corticosteroid. These two cases are reported herein with a review of corresponding literature.
Adult ; Alopecia ; Canada* ; Diarrhea ; Ectoderm ; Humans ; Intestinal Polyposis ; Malnutrition ; Prognosis