The pharmacologic actions of ketamine in human volunteers were reported by Domino et al in 1965 and it was used in 130 patients by Corssen Domino in 1966. Chodoff and Stella were the first to investigate ketamines suitabilityas an in anesthetic in childbirth. Since then several authors reported that ketamine has several advantages over conventional anesthetics in obstetric anesthesia. Ketamine was used as the sole anesthetic agent for forceps delivery in 50 women who were selected randomly. Ketamine was administered intravenously just before delivery in doses of 30 to 60 mg and after delivery dosage was not limited, The following results were observed: 1) During delivery, a rapid and intense analgesic effect was sufficiently maintained with a small dose of ketamine. 2) With the use of ketarnine it is possible to shorten the second stage of labor with a short induction-delivery-interval because of the advantages of forceps delivery. 3) Ketamine could be used without intubation during with a short fasting time because protective laryngeal quate airway could be maintained. 4) Ketamine did not appear to induce an increase of Very. delivery even in patients and pharyngeal reflexes and an ade uterine bleeding during or after deli 5) The use of ketamine during delivery appeared to have almost no affect on the Apgar score. 6) The use of ketamine was accompanied by mild complications but they were not significant.
Anesthesia, Obstetrical* ; Anesthetics ; Apgar Score ; Fasting ; Female ; Gagging ; Healthy Volunteers ; Humans ; Intubation ; Ketamine* ; Parturition ; Pharmacologic Actions ; Surgical Instruments ; Uterine Hemorrhage

Porphyromonas gingivalis is strongly implicated in the pathogenesis of adult periodontitis, the major cause of tooth loss in adults. Use of an antibacterial agent controlling P. gingivalis as a periodontal therapeutic agent has been rationalized. The present study was performed to observe the antibacterial effect of inorganic polyphosphates (polyP) on P. gingivalis. P. gingivalis 2561 was grown in half-strength brain-heart infusion broth containing hemin and vitamin K with or without polyP. Minimal inhibitory concentration (MIC) of polyP with various chain lengths was determined by measuring the absorbance of the grown cells at 540 nm. MIC of polyP for the bacterium was determined to be 0.05%. The effect of polyP with a chain length of 75 (polyP 75) was further examined. PolyP 75 added to the growing culture of P. gingivalis at its exponential phase was as effective in inhibiting the growth of P. gingivalis as polyP 75 added at the very beginning of the culture. More than 99% of the cells lost their viability determined by viable cell count when polyP 75 was added to the culture of growing P. gingivalis at the concentration of 0.06%, suggesting that polyP 75 has a bactericidal effect on the bacterium. Intracellular nucleotide release from the cells was increased by approx. 20% in the presence of polyP 75 but was not reversed by the addition of divalent cations like Ca++ and Mg++. Under the transmission electron microscope, only a small number of the growing P. gingivalis cells were actually lysed. However, the majority of the cells appeared to be atypical in their shape, demonstrating accumulation of highly electron-dense granules and bodies of condensed nucleic acid-like material in the cytoplasm. In the presence of polyP 75, the protein profile of P. gingivalis was changed as determined by SDS-polyacrylamide gel electrophoresis and immunoblot, and the proteolytic activity of the bacterium demostrated on the zymograms was decreased. The overall results suggest that polyP have a strong bactericidal activity against P. gingivalis in which lysis in relation to chelation may not play the major role but unknown mechanism that possibly affects the viability of the bacterium may be involved. PolyP may be used as an agent for prevention and treatment of periodontitis.
Adult ; Cations, Divalent ; Cell Count ; Chronic Periodontitis ; Cytoplasm ; Electrophoresis ; Hemin ; Humans ; Periodontitis ; Polyphosphates* ; Polyps ; Porphyromonas gingivalis* ; Porphyromonas* ; Tooth Loss ; Vitamin K

OBJECTIVE: Psychiatric disorders such as depression, anxiety and alcohol dependence are associated with serotonin metabolism. We assessed the methylation level of the serotonin transporter (5-HTT) promoter region in control and alcohol dependent patients. METHODS: Twenty seven male patients who met the Diagnostic and Statistical Manual of Mental Disorder IV (DSM-IV) criteria for alcohol dependence were compared with fifteen controls. Polymerase chain reaction (PCR) assays of bisulfate-modified DNA were designed to amplify a part of the CpG island in the 5HTT gene. Pyrosequencing was performed and the methylation level at seven CpG island sites was measured. RESULTS: We found no differences in the methylation patterns of the serotonin transporter linked promoter region (5-HTTLPR) between alcohol-dependent and control subjects. CONCLUSION: Our negative finding may be because 5-HTT epigenetic variation may not affect the expression for 5-HTT or there may be other methylation site critical for its expression. To find out more conclusive result, repeating the study in more methylation sites with a larger number of samples in a well-controlled setting is needed.
Alcoholism ; Anxiety ; CpG Islands ; Depression ; DNA ; Epigenomics ; Humans ; Male ; Mental Disorders ; Methylation ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; Serotonin ; Serotonin Plasma Membrane Transport Proteins

No abstract available.
Animals ; Hypothermia* ; Rats*

BACKGROUND/AIMS: Gout is a common inf lammatory arthritis triggered by the crystallization of uric acid in the joints. Serum uric acid levels are highly heritable, suggesting a strong genetic component. Independent studies to confirm the genetic associations with gout in various ethnic populations are warranted. We investigated the association of polymorphisms in the ABCG2 and SLC2A9 genes with gout in Korean patients and healthy individuals. METHODS: We consecutively enrolled 109 patients with gout and 102 healthy controls. The diagnosis of gout was based on the preliminary criteria of the America College of Rheumatology. Genomic DNA was extracted from whole blood samples. We identified single nucleotide polymorphism (SNP) changes in the ABCG2 and SLC2A9 genes using a direct sequencing technique. rs2231142 in ABCG2 and rs6449213 and rs16890979 in SLC2A9 and nearby regions were amplified by polymerase chain reaction. RESULTS: Patients with gout had significantly higher A/A genotype (29.3% vs. 4.9%, respectively) and A allele (52.8% vs. 26.5%, respectively) frequencies of rs2231142 in ABCG2 than did controls (chi2 = 29.42, p < 0.001; odds ratio, 3.32; 95% confidence interval, 2.11 to 5.20). We found novel polymorphisms (c.881A>G and c.1002+78G>A) in the SLC2A9 gene. The univariate logistic regression analysis revealed that the c.881A>G and c.1002+78G>A SNPs were significantly higher in patients than in controls. CONCLUSIONS: We demonstrated a significant association between rs2231142 in the ABCG2 gene and gout and identified novel SNPs, c.881A>G and c.1002+78G>A, in the SLC2A9 gene that may be associated with gout in a Korean population.
ATP-Binding Cassette Transporters/*genetics ; Arthritis, Gouty/blood/diagnosis/ethnology/*genetics ; Asian Continental Ancestry Group/genetics ; Biomarkers/blood ; Case-Control Studies ; Chi-Square Distribution ; Gene Frequency ; Genetic Association Studies ; Genetic Predisposition to Disease ; Glucose Transport Proteins, Facilitative/*genetics ; Haplotypes ; Humans ; Logistic Models ; Neoplasm Proteins/*genetics ; Odds Ratio ; Phenotype ; *Polymorphism, Single Nucleotide ; Republic of Korea ; Risk Factors ; Uric Acid/blood

PURPOSE: This study was performed to identify the mediating effect of empowerment in the relationship between head nurses' servant leadership and organizational commitment among clinical nurses. METHODS: A cross-sectional survey design was used. A convenience sample of 249 nurses was recruited from three hospitals located in J province, South Korea. Data were collected by self-report questionnaires, including general characteristics, servant leadership, empowerment, and organizational commitment. Data were analyzed with descriptive statistics, Pearson correlation coefficients, independent t-test, One-way ANOVA, and linear regression using the SPSS 22.0 program. RESULTS: Head nurses' servant leadership perceived by clinical nurses significantly influenced their organization commitment. Empowerment partly mediated the effect of servant leadership on organizational commitment. CONCLUSION: The findings suggest the importance of improving nurse managers' servant leadership and empowering nurses to increase their organizational commitment.
Cross-Sectional Studies ; Head* ; Korea ; Leadership* ; Linear Models ; Negotiating* ; Power (Psychology)*

The first step in the commissioning procedure of a treatment planning system is always verification of the basic beam data. In this work, we have measured PDD curves and beam profiles between 1x1 cm2 and 40x40 cm2. In an attempt, Pinnacle 7.4f detect discrepancies between predicted dose distribution and delivered dose distribution. The discrepancies between measurement data and caculation data was found. The delivered dose was underestimated in field but overestimated out of field. The Dmax depth of 1x1 cm2 was reduced about 2 mm. For the larger field size (> or = 4x4 cm2), the beam profile and PDD curve showed good agreement between measurement data and calculation data.

Ureterocele, congenital dilatation of the terminal or intramural portion of the ureter, may be classified as either simple or ectopic and has a broad spectrum of presentation, anatomy and treatment must be individualized. From February, 1986 to March, 1994, our experiences with 8 single system ureteroceles and 12 duplex system ureteroceles were reviewed. Their presentation, radiographic findings, operative management and postoperative results were discussed. The patients in this series were distribution from 1 year old to 66 years old and the male and female ratio was 4:16. The most presenting symptom was flank pain, affecting 7 cases, and ureterocele was associated with ureteral stone: 7 cases, VUR: 2 cases, renal cyst: 1 case, and IgA nephropathy:1 case. In 8 cases of single system ureterocele, Transurethral incision(TUI) of ureterocele in 1 case, ureterocelectomy with ureteroneocystostomy in 3 cases were done. In 12 cases of duplex system ureterocele, TUI of ureterocele in 2 cases, heminephrectomy with partial ureterocelectomy in 2 cases were done. After operation was done, there was no evidence of complication. We conclude that the surgical approach to the problems associated with a ureterocele is modified by patient age, renal anomaly and the pathological condition of the lower urinary tract.
Aged ; Dilatation ; Female ; Flank Pain ; Humans ; Immunoglobulin A ; Male ; Ureter ; Ureterocele* ; Urinary Tract

Clinical analysis and review were made on the 65 aneurysmal patients who had intraventricular hemorrhage(IVH) confirmed on preoperative brain CT among a total of 268 patients operated on intracranial aneurysms. The study was made on aneurismal sites, the severity of IVH, postoperative ventricular dilatation and the result of treatment. The frequency of IVH among the 268 aneurysmal patients with direct clipping was 24.3%. Aneurysms originated from anterior communicating artery had the highest frequency(49.2%), followed by from posterior communicating artery(15.4%). On the severity of IVH, the frequency of severe IVH was 49.2%, the highest. The second was mild IVH, 32.3%. Forty five percent accounted for ventricular dilatation. By ventricular size index, mild dilatation was 7.7%;moderate, 13.8%;and severe dilatation, 20%. 11 patients(16.9%) undersent CSF shunting procedure. The treatment for the 65 aneurysmal patients with IVH ended in:survivor, 58 patients(98.3%), good recovery, 32.3% and 7 deaths, the rate of mortality was 10.7%.
Aneurysm ; Arteries ; Brain ; Dilatation* ; Hemorrhage* ; Humans ; Hydrocephalus ; Intracranial Aneurysm* ; Mortality

The authors reviewed retrospectively the records of 27 cases of posterior fossa tumor in patients below the age of 60 among the 387 brain tumor cases admitted to Kyungpook University Hospital during the last 10 years. The posterior fossa tumors were 34.2% of the total 79 cases of pediatric brain tumors. The most frequently involved age group was 6~8 years. Headache(40.7%) and vomiting(37.0%) were the most common initial presenting symptoms. Cerebellar signs, Cranial nerve palsy and motor weakness were found in 66.7%, 9.6% and 11.1% of the cases respectively. Cerebellar midline was the most frequently involved site(63.0%). In order of frequency, the pathological diagnoses were medulloblastoma, ependymoma, primitive neuroectodermal tumor(PNET), and astrocytoma. Hydrocephalus was noted in 70.4%, and shunt surgery was done in 28.6%. Surgery followed by radiation therapy was used in most of the cases.
Astrocytoma ; Brain Neoplasms ; Child* ; Cranial Nerve Diseases ; Diagnosis ; Ependymoma ; Gyeongsangbuk-do ; Humans ; Hydrocephalus ; Infratentorial Neoplasms* ; Medulloblastoma ; Neural Plate ; Retrospective Studies