For demonstrating the changes of lectin biding pattern in the developing rat, nine biotinylated lectin were applied with ABC method. The affinities for SBA, PNA, RCA-1, sWGA, and Con A were observed on 15 day fetal rat, and an additional UEA-1 affinty on 17 day fetal rat was detected. From the next developing rats, lectin binding pattern was signicantly altered and these changes were greater in suckling rats than others. The notable changes of the duodenal mucosa in developing rats were as follows : A new affinities for SBA, sWGA and UEA-1 in the villous columnar cells and PNA, RCA-1, sWGA and Con A in the crypt columnar cells were detected from suckling rats. The SBA, PNA, BSL-1, RCA-1, sWGA and UEA-1 affinities in the Golgi zone of villous columnar cells showed a tendency to increase from the suckling rats, and a new affinities for SBA, PNA, BSL-1, RCA-1 and sWGA in that of crypt columnar cells revealed from same rats. All lectin affinities used except DBA and Con A were observed in the brush border and maintained a higher level until adults, however, no BSL-1 affinity was detected from the weaning rats. The UEA-1 affinity of goblet cells was detected from 19 day fetal rat and not observed from the weaning rats. And the affinity for DBA and SBA of the goblet cells was demonstrated from the suckling and weaning rats respectively. All lectin affinities examined were observed in the duodenal gland and increased from the suckling rats.
Adult ; Animals ; Duodenum ; Goblet Cells ; Humans ; Microvilli ; Mucous Membrane* ; Rats* ; Weaning

According to repeated measurements and correction procedures, the accuracy of the phase-shifting profilometry was developed. At first, after 20 final models for maxillary complete denture were duplicated, the mucosa surfaces of models were measured with the phase-shifting profilometry and each mirror view of these was calibrated. Maxillary cats were divided into 4 groups of 5 casts per each, and wax dentures with 2 sheets of baseplate wax and artificial teeth were made and then cured according to the curing method of each group. Group I ; quick curing with QC-20 acrylic resin Group II ; 9 hour curing with QC-20 acrylic resin Group III ; SR-Ivocap system Group IV ; metal base and quick curing with QC-20 acrylic resin After curing, polishing, and storing at 37degrees C n saline for 30 days, the forms of the impression surface of the dentures were measured with the phase-shifting profilometry. Then, the impression surface form of each denture was placed in the optimal position of comparison with the mirror view of the same final cast. The amount and direction of distortion of each denture was analyzed and the effects of polishing and storage in each denture were compared. The obtained results were as follows : 1. In Group I, the denture was observed as the appearance distorted in the opposite direction of the mucosa and the postero-lateral part of palatal portion of the denture was observed as the appearance separated from the mucosa. Also, the buccal flanges of the denture were observed as the appearance distorted in the direction of the mucosa. 2. In Group II, the postero-lateral part of palatal portion of the denture was observed as the appearance separated slightly from the mucosa. The bilateral buccal flanges of denture were observed as the appearance distorted severely in the direction of the mucosa. 3. In Group III, the bilateral part of the residual ridge crest portions and the buccal flanges of the denture were observed as the appearance distorted in the direction of the mucosa, and specially, the buccal flanges of the maxillary tuberosities were distorted severely. 4. In Group IV, the acrylic resin base of the buccal portion of the denture was observed as the appearance distorted in the opposite direction of the mucosa. 5. The phase-shifting profilometry, done with repeated measurements and correction procedures, was effective in comparing the amount and direction of distortion at every position after the laboratory work and the delivery of maxillary complete denture.
Animals ; Cats ; Denture, Complete ; Dentures* ; Mucous Membrane ; Tooth, Artificial

No abstract available.
Gastrin-Secreting Cells* ; Gastrins* ; Gastrointestinal Tract*

Estrous cycle -related histological and histochemical changes in the vaginal epithelium of mature female rats were studied with PAS (periodic acid Schiff) alcian blue pH 2.5 and biotinylated lectins (DBA, SBA, PNA, BSL -1, sWGA, UEA -1, RCA -1, Con A and LCA).The prominent characteristic changes that occured during the estrous cycle were mucinous transformation in proestrus and cornification in estrus. In proestrus, the superficial mucinous cells of the epithelium were increased in number and enlarged in size, and the amount of acid and neutral mucosubstances was more increase in proestrus than in diestrus and metestrus. About the binding pattern of all lectins examined to the superficial mucinous cells, in diestrus, the binding pattern of these cells showed a similar affinity as in metestrus with intense DBA and UEA -1 reactivity. In proestrus, however, these cells were reactive with seven lectins examined except LCA and PNA, and DBA, SBA, BSL -1, RCA -1 and UEA -1 reacted more strongly than in diestrus and metestrus. In estrus, the superficial cornified cell layers showed a weak reactivity of SBA, BSL -1 and PNA. In diestrus and metestrus, the mucinous cells in the intermediate layers of the basal portion of vaginal fold stained with eight lectins examined except LCA and showed the same binding pattern to the superficial mucinous cells. About the distribution of glycoconjugates in the intermediate layer, the upper spindle cells showed different binding pattern according to the estrous stages. In diestrus, estrus, and metestrus, these cells showed a affinity for all lectins examined. In proestrus, however, DBA and PNA staining were not observed, and stained more intensely with sWGA, SBA and UEA -1, and less intensely with BSL -1 and RCA - 1. In estrus, DBA and PNA reactivity reappeared as trace, and RCA -1 and sWGA reactivity increased. In metestrus, sWGA reactivity reduced and BSL -1 and UEA -1 increased continually. The lower rounded cells of the intermediate layers stained with all lectins examined in estrus, with six lectins examined except Con A, DBA and UEA -1 in proestrus and with five lectins examined except DBA, UEA -1, sWGA and BSL -1 in diestrus and metestrus. BSL -1 reactivity for the layers increased in proestrus, estrus and metestrus, and PNA reactivity increased in estrus and reduced in metestrus. The basal layer of the vaginal epithelium showed different binding pattern to the different portion of vagina, and showed faint staining of BSL -1, SBA and RCA -1, and moderately staining of BSL -1 in proestrus and estrus. In conclusion, alpha /-N -acetyl -D -galactosamine, alpha /-D -galactose and alpha -L -fucose participate in the mucinous transformation of the vaginal epithelium, and beta -N -acetyl -D -glucosamine participates in the cornification of the vaginal epithelium.
Alcian Blue ; Animals ; Diestrus ; Epithelium* ; Estrous Cycle* ; Estrus ; Female ; Glycoconjugates ; Humans ; Hydrogen-Ion Concentration ; Lectins ; Metestrus ; Mucins ; Proestrus ; Rats* ; Vagina

Nevus lipomatosus cutaneous superficialis is a very rare skin disease which usually is present from birth. This uncommon condition is due to collections of ectopic. fat cells within the upper and mid dermis. There are two clinical varieties. The first is a lesion of zonal distribution, present from birth or childhood, usualIy on the buttocks or the lower back. The second form, a dome or sessile, papule, begins. in adult life and is less restricted in distribution. Cerebriform plaques tend to form from the coalescence of soft, yellowish papules. The authors observed two cases of typical nevus lipomatosus cutaneous superficialis. The first case is a 25-year-old female who has had asymptomatic, soft, skin colored, and huge confluent nodules on the right infragluteal fold and upper thigh for 15 years; and the second case is a 15-year-old male who has had multiple, skin colored and soft papules or nodules in zosteriform distribution affecting the right side of the lower back and coccygeal area and right buttock for 4 years. The authors made the diagnosis of nevus lipomatosus cutaneous superficialis from the characteristic clinical and histopathological findings.
Adipocytes ; Adolescent ; Adult ; Buttocks ; Dermis ; Diagnosis ; Female ; Fluconazole ; Humans ; Male ; Nevus* ; Parturition ; Skin ; Skin Diseases ; Thigh

This is a case of laryngeal obstruction with progressive dyspnea and hoarseness due to cricoary tenoid arthritis following endotracheal intubation, which was performed for surgery in a 41 year old male suffering from liver abscess.
Adult ; Arthritis* ; Dyspnea ; Hoarseness ; Humans ; Intubation, Intratracheal* ; Liver Abscess ; Male

No abstract available.
Causality* ; Humans ; Infant, Newborn* ; Pneumothorax*

No abstract available.
Intussusception* ; Peutz-Jeghers Syndrome*

Congenital lobar emphysema has the clinical features of an air block' syndrome with- out the evidence of pulmonary infection or intrabronchial foreign body. The hyperinflated lung causes a compression of uninvolved lobes creating respiratory distress, cyanosis within the first weeks of life. We experienced a case of congenital lobar emphysema diagnosed incidentally by chest reontgenogram in an infant with frequent upper respiratory infection within a few weeks of life. Chest X-ray revealed extensive emphysematous changes in the left upper lobe, shifting of heart and medistinum to the right and compression of the right lung. Respiratory distress, cyanosis and chest wall retraction ensued and left upper lobe Lobectomy was performed successfully.
Cyanosis ; Emphysema* ; Foreign Bodies ; Heart ; Humans ; Infant ; Lung ; Thoracic Wall ; Thorax

The authors have experienced, during general anesthesia with methoxyflurane-nitrous oxide- oxygen for explorative laparatomy to perform appendectomy, a case of acute pulmonary edema, which is rare and it's etiology is various. The patient had no specific past or family history and the only predisposing factor was myocardial ischemia caused by cardio-vascular collapse due to severe dehydration. In this case, the authors believed that pre-existing myocardial ischemia, rapid transfusion of 5% dextrose and myocardial depressants (methoxyflurane and d-tubocurarine) were the causative factors. For this reason at least, myocardial depressants should be avoided in severely dehydrated patients. There are many preventive measures and treatments for acute pulmonary edema during general anesthesia. As in this case, we believe that prompt recognition and attention by the anesthesiologists are the most important preventive and therapeutic measures.
Anesthesia, General* ; Anti-Arrhythmia Agents ; Appendectomy ; Causality ; Dehydration ; Edema* ; Glucose ; Humans ; Myocardial Ischemia ; Oxygen ; Pulmonary Edema