2.Preimplantation Development and Apoptosis of Mouse Embryos in the Medium Containing Extracellualr Matrix.
Byung Moon KANG ; In Pyo SOHN ; Byung Mok CHUNG ; Kyoo Wan CHOI ; Myung Chan GYE
Korean Journal of Fertility and Sterility 2000;27(3):253-259
OBJECTIVE: To verify the effect of Matrigel, a ECM complex from Engelbreth-Holm-Swarm(EHS) mouse sarcoma on the preimplantation development and apoptosis of mouse fertilized eggs. METHOD: Late Pronucleus stage eggs were cultured through the blastocyst stage in the presence of Matrigel (0.5%, v/v). Characteristics of apoptosis and cell number assessed by Hoecst staining and TUNEL labeling at the blastocyst stage, respectively. RESULTS: Morphological development, number of cells per embryo was significantly increased but rate and number of TUNEL positive nuclei of the embryo were decreased in the presence of Matrigel. CONCLUSION: This result suggested that at low concentration of Matrigel improves both viability and morphological development in the preimplantation mouse embryos.
Animals
;
Apoptosis*
;
Blastocyst
;
Cell Count
;
Eggs
;
Embryonic Structures*
;
In Situ Nick-End Labeling
;
Mice*
;
Ovum
;
Sarcoma
;
Zygote
3.Percutaneous transhepatic biliary drainage: an analysis on 72 cases of internal drainage
Jae Hyung PARK ; Byung Ihn CHOI ; Chan Sup PARK ; Kyu Bo SUNG ; Byung Hee LEE ; Man Chung HAN
Journal of the Korean Radiological Society 1986;22(3):311-316
A total of 72 cases of internal biliary drainage procedure has been done percutaneously at Departement ofRadilogy, Seoul Natinal Universtiy Hospital for recent 4 and half years since August 1981. Five different types ofinternal drainage including endoprosthesis were applied to various conditions with different obstruction levels.The different method of procedure in each type of internal drainage was described and the results were analysed.1. Among the clinical diagnosis in 72 cases, carcinoma of bile duct was in 37 cases as the most frequent one. Thenext was pancreas head carcinoma in 16 cases, followed by metastatic carcinoma and other disease. 2. Type Iinternal drainage, classical internal and external type with distal end in duodenum, was applied in 43 cases asthe most comon one. Type II, modification of type I with distal end in CBD, was applied in 17 cases. Type III,endoprosthesis distal end into duodenum, was applied in 7 cases. Type IV, endoprosthesis just across theobstruction in bile duct, was applied in 2 cases, Type V, combined type of any internal drainage with externaldrainage, was applied in 3 cases. 3. According to various obstruction level, prefered type could be selected. Forproximal obstruction type II and IV were preferred and for distal obstruction type III was chosen. However, type Icould be applied in any situation. 4. Early complication occurred in 9 cases(12.5%). Clinical imporvement withdecrease in bilirubin level was observed 17 of 21 cases in which follow-up data was available over 2 months. 5.Though the experience with those 72 cases it is recommended that appropriate type of internal biliary drainageshould be selected according to prognosis, obstruction level and clinical findings of each patient.
Bile Ducts
;
Bilirubin
;
Diagnosis
;
Drainage
;
Duodenum
;
Follow-Up Studies
;
Head
;
Humans
;
Methods
;
Pancreas
;
Prognosis
;
Seoul
4.Femoral Shaft Fracture in Klippel–Trenaunay–Weber Syndrome Patients – What to Do to Reduce Bleeding Risk: A Case Report
Byung-Chan CHOI ; Byung-Woo MIN ; Kyung-Jae LEE
Hip & Pelvis 2022;34(4):262-268
A fracture of the affected extremity in patients with Klippel–Trenaunay–Weber syndrome can be fatal due to massive bleeding and show poor results. A 42-year-old male presented with an old fracture of the right femoral shaft with metal failure. We planned an operation to remove the previously fixed plate and to perform re-fixation using an intra-medullary nail. Preoperative angiography was performed and the arteriovenous malformations were embolized in order to reduce the risk of bleeding. After angiography, the previously fixed plate was removed.After the operation, a second angiography was performed immediately and the venous malformation was embolized. One week after the first operation, a second operation was performed in order to reduce the fracture and to perform re-fixation using an intramedullary nail. The patient is being followed without major complication over a period of seven years after surgery. We recommend careful planning of preoperative and postoperative angiography and embolization in order to reduce the risk of bleeding in patients with Klippel–Trenaunay–Weber syndrome.
5.A BRW Stereotaxic Biopsy of Brain Stem Glioma.
Seung Chan BEAK ; Byung Yon CHOI ; Choong Bae MOON ; Yong Chul CHI ; Soo Ho CHO
Yeungnam University Journal of Medicine 1986;3(1):343-349
Histopathological diagnosis of brain stem glioma should be performed for the purpose of the determination of its management and clinical course, but its surgical biopsy has been followed by high mortality and morbidity. We performed the tissue sampling for histological examination with BRW stereotaxic system under local anesthesia successfully.
Anesthesia, Local
;
Biopsy*
;
Brain Stem*
;
Brain*
;
Diagnosis
;
Glioma*
;
Mortality
6.Role of Redistribution and 24 Hour Reinjection Images to assess Myocardial Viability in Patients with Acute Myocardial Infarction.
Seok Nam YOON ; Moon Sun PAI ; Chan H PARK ; Myung Ho YOON ; Byung Il CHOI
Korean Journal of Nuclear Medicine 1998;32(4):325-331
PURPOSE: We evaluated the importance of redistribution and 24 hour reinjection images in Tl-201 SPECT assessment of myocardial viability after acute myocardial infarction (AMI). MATERIALS AND METHODS: We performed dipyridamole stress-4 hour redistribution-24 hour reinjection Tl-201 SPECT in 43 patients with recent AMI (4-16 days). The myocardium was divided into 16 segments and perfusion grade was measured visually with 4 point score from 0 to 3 (absent uptake to normal uptake). A perfusion defect with stress score 2 was considered moderate. A defect was considered severe if the stress score was 0 or l (absent uptake or severe perfusion decrease). Moderate defect on stress image were considered viable and segments with severe defect were considered viable if they showed improvement of 1 score or more on redistribution or reinjection images. We compared the results of viability assess-ment in stress-redistribution and stress-reinjection images. RESULTS: On visual analysis, 344 of 688 segments (50%) had abnormal perfusion. Fifty two (15%) had moderate perfusion defects and 292 (85%) had severe perfusion defects on stress image. Of 292 severe stress defects, 53 were irreversible on redistribution and reversible on reinjection images, and 15 were reversible on redistribution and irreversible on reinjection images. Two hundred twenty four of 292 segments (76.7%) showed concordant results on stress-redistribution and stress-reinjection images. Therefore 24 hour reinjection image changed viability status from necrotic to viable in 53 segments of 292 severe stress defect (18%). However, myocardial viability was underestimated in only 5% (15/292) of severe defects by 24 hour reinjection. CONCLUSION: The 24 hour reinjection imaging is useful in the assessment of rnyocardial viability. It is more sensitive than 4 hour redistribution imaging. However, both redistribution and reinjection images are needed since they complement each other.
Complement System Proteins
;
Dipyridamole
;
Humans
;
Myocardial Infarction*
;
Myocardium
;
Perfusion
;
Tomography, Emission-Computed, Single-Photon
7.Clinical study of Outcome and Predisposing Factors for Spontaneous Pneumothorax in the Neonates.
Byung Joo CHOI ; Jo Won JUNG ; Seon Chan BAE ; Moon Sung PARK ; Jeong In YANG
Korean Journal of Perinatology 2000;11(4):438-444
No abstract available.
Causality*
;
Humans
;
Infant, Newborn*
;
Pneumothorax*
8.The study of soft tissue changes in orthognathic surgery patients.
Byung Wook CHO ; Yong Chan LEE ; Baek Jin KOH ; Chang Won CHOI
Journal of the Korean Association of Oral and Maxillofacial Surgeons 1991;17(2):40-43
No abstract available.
Humans
;
Orthognathic Surgery*
9.A Case of Pulmonary Squestration.
Byung Sook PARK ; Jin Oh KIM ; Beyong Sang CHOI ; Chan Uhng JOO ; Dae Yeol LEE
Journal of the Korean Pediatric Society 1988;31(10):1390-1393
No abstract available.
10.Prognostic evaluation of regional lymph node morphology in uterine cervical cancer.
Dong Hee CHOI ; Byung Seok LEE ; Tchan Kyu PARK ; Chan Il PARK
Korean Journal of Obstetrics and Gynecology 1991;34(11):1583-1591
No abstract available.
Lymph Nodes*
;
Uterine Cervical Neoplasms*