No abstract available.
Breast Neoplasms* ; Breast* ; Eflornithine* ; Estrogens ; Humans* ; MCF-7 Cells ; Phosphorylation* ; Polyamines

BACKGROUNDS: Polyamines are known to be essential for cell growth and differentiation. Recently, possible roles of the polyamine in signal transduction as neurotransmitter, modulator, or second messenger are suggested in many studies. Furthermore, it is widely studied that possible roles of polyamine are involved in the action of hormone. Thus, it was to investigate the effect of polyamines in the cell proliferation and secretion of GH from the GH cells. METHODS: Cells(5*10 cells/mL) were incubated for 3 days in DMEM containing test drugs and labeled with 20pCi/mL of [S]-methionine for 2 hr. Proteins secreted into the medium were separated by 13% SDS-gel electrophoresis, then autoradiography was performed to identify radiolabeled proteins. [S]-methionine labelled GH was identified by radioimmuno-precipitation. Total protein synthesis was determined from the radioactivity of the cell homogenate by liquid scintillation counter. The intracellular polyamine content was determined by HPLC. RESULTS: Externally added polyamines(putrescine, spermidine, spermine) induced cell proliferation in a dose-dependent manner at proper concentrations, specifically 50pM putrescine increased GH secretion, DFMO or MGBG, which is polyamine biosynthetic inhibitor, inhibited GH secretion in a dose-dependent fashion, In the cells treated with 20mM or 0.01mM MGBG, total protein synthesis were decreased only to 90 or 76% of the control levels and cell proliferation was also slightly inhibited. However the secretion of GH was severely blocked to 37% or 35% of the control. Hydrocortisone at 5 pM stimulated the secretion of GH to 153% of basal secretion, also doubled intracellular putrescine content. CONCLUSION: The present data show that externally added polyamines induced cell proliferation and GH secretion. Also, extemally added putrescine stimulated GH secretion significantly. GH secretion was inhibited by polyamine metabolic inhibitor in a dose-dependent manner and polyamine metabolic inhibitors, at proper concentrations, specifically blocked GH secretion without any significant influence on the total protein synthesis. The above results imply the involvement of polyamine in GH secretion.
Autoradiography ; Cell Proliferation ; Chromatography, High Pressure Liquid ; Electrophoresis ; Growth Hormone ; Hydrocortisone ; Mitoguazone ; Neurotransmitter Agents ; Polyamines ; Putrescine ; Radioactivity ; Scintillation Counting ; Second Messenger Systems ; Signal Transduction ; Spermidine

A 28-year-old woman had a 13-month history of skin-colored, well-circumscribed, hyperkeratotic plaques on both heels. Histological examination showed hyperkeratosis and acanthosis in the epidermis. Special stain for elastic tissue revealed marked fragmentation, diminution and thickening of elastic fibers in the dermis. We report a rare case with the lesions of acrokeratoelastoidosis on both heels.
Adult ; Dermis ; Elastic Tissue ; Epidermis ; Female ; Heel ; Humans

Fracture of capitellum humeri, so called Kocher fracture, was first described by Haln in 1853. It is rare and has been occured 0.5% to 1% of incidence of all elbow fracture. The mechanism of this fracture mostly results when one tries to break a fall and lands on the hand with the elbow in some degree of flexing or falls directly on the elbow in a position of full flexion. This case had been occured in a secondary motion
Accidental Falls ; Athletes ; Elbow ; Hand ; Humans ; Incidence ; Methods ; Weight Lifting

No abstract available.
Delusions* ; Schizophrenia*

Distal radial fractures are recognized as very complex injuries with a variable prognosis that depends upon the fracture type and the treatment method. The purpose of this study is to compare radiological and clinical result between external fixation and external fixation with primary bone graft in intraarticular comminuted fracture of distal radius. From January 1993 to December 1995, 32 patients with comminuted intraarticular fractures of the distal radius were treated using a external fixation. 15 cases were treated with ligamentotaxis without bone graft and 17 cases were treated with additional primary bone graft. In last follow-up examination, the mean loss of radial length, radial inclination and volar tilt were less in with bone graft group than without bone graft group. According to Demerit point system Excellent to good results were obtained in 88.2% in cases of external fixation and bone graft group, 66.6% in cases of only extemal fixation group. External fixation combined with primary bone graft is more useful method for the reconstruction and treatment of comminuted intraarticular fractures of the distal radius than closed reduction and external fixation because cancellous bone graft not only provides early mechanical stability but also helps to reduce and supports the depressed intraarticular fragments. Furthermore, this method not only prevents late collapse of fracture but also enhance fracture healing.
External Fixators* ; Follow-Up Studies ; Fracture Healing ; Fractures, Comminuted ; Humans ; Intra-Articular Fractures* ; Prognosis ; Radius* ; Transplants*

In 1941, Jaffe and coworkers studied a lesion with histologic appearance of fibrous stroma, pigmented deposition and histiocytic infiltration as well as giant cell, for which they named pigmented villonodular synovitis, bursitis and tenosynovitis. Thereafter many authors have discussed etiology, clinical and radiological features, pathology and treatment regarding the disease. Almost all, this disease is monoarticular and knee joint is most common site. We experienced a case of localized pigmented villonodular synovitis involving an shoulder joint which was treated by local excision and obtained good result.
Bursitis ; Giant Cells ; Knee Joint ; Pathology ; Shoulder Joint ; Shoulder ; Synovitis, Pigmented Villonodular ; Tenosynovitis

No abstract available.
Anaphylaxis* ; Histamine Release* ; Histamine* ; Mast Cells*

No abstract available.
Axis, Cervical Vertebra*

Traumatic dislocation of the knee joint occurs very infrequently, but is one of the true emergencies in the orthopedic field. It is a serious injury, associated with extensive soft tissue demage and the danger of neurological and vascular involvement. There is a lot of theories about the mechanism of injury, the treatment and the incidence of complications. Generally they accept that in irreducible cases operative reduction is essential, but there is no uniformity of opinion on the treatment of the uncomplicated cases. This paper is based on 7 traumatic knee dislocations treated at Kang Nam Sacred Heart Hospital, Hallym College during the period from January 1980 till December 1984. The results of the study are as follows: 1. The patients are mostly injured due to the traffic accident and show even age distribution from twenties to fifties. 2. 5 cases out of 7, reduced at our hospital, were all anteriorly dislocated. 3. The posterior capsule and the posterior cruciate ligament were injured in all cases. 4. There were no significant differences between primary repair of soft tissues and the late reconstruction.
Accidents, Traffic ; Age Distribution ; Clinical Study ; Clothing ; Dislocations ; Emergencies ; Heart ; Humans ; Incidence ; Knee Dislocation ; Knee Joint ; Knee ; Orthopedics ; Posterior Cruciate Ligament