No abstract available.
Neurofibroma*

To know the amplification pattern according to relative concentration ratio in mixed samples, two STRloci, vwF locus and MBP locus and two VNTR loci, D1S80 locus and d17S5 locus were amplified in DNA with various concentration of two individuals were easily identified. But when the concentration of one person were lowered to 1/20-1/40 of the other's the intensity of product bands diminshed and hardly discernible. Also different amplification efficiency according to the template length was noted, especially in VNTR loci. Using automatic sequencer and RFLP scan program, the intensity OD of each PCR product band could be calculated, and this correlates the felative amplification efficiency of each allele. By using this we could construct quantitative PCR for the mixed samples. This could be used in practical case work for forensic purpose, and also be a valuable candidate for 'chimerism detection' in case of bone marrow transplatation.
Alleles ; Bone Marrow ; DNA ; Humans ; Minisatellite Repeats ; Polymerase Chain Reaction* ; Polymorphism, Restriction Fragment Length

No abstract available.
Friction* ; Hyperpigmentation* ; Skin Diseases*

No abstract available.
Paralysis* ; Radial Nerve*

No abstract available.
Carcinoma, Basal Cell ; Facial Dermatoses* ; Keratosis, Actinic ; Skin*

No abstract available.
Herpes Simplex* ; Immunocompromised Host* ; Immunosuppression ; Simplexvirus*

No abstract available.
Porokeratosis*

Nontuberculous mycobacteria usually cause systemic disease and often appear as a primary pulmonary infection. However, a cutaneous lesion may be the first or only sign of infection. The most frequent human diseases caused by Mycobacterium fortuitum are cutaneous or soft tissue infections, usually preceded by injection, trauma or surgery. We report a case of a cutaneous lesion due to Mycobacterium(M.) fortuitum occuring in a 50-year-old man with a subcutaneous flat nodule on his left anterior tibia. He had received a small injury to his left shin in a swimming pool. The culture from the skin biopsy specimen on Lowenstein-Jensen medium yielded clolnies after 5 days. The microorganism was subsequently identified as M. fortuitum by selected biochemical reactions. Therapy was instituted with minocycline for 5 months.
Biopsy ; Humans ; Middle Aged ; Minocycline ; Mycobacterium fortuitum* ; Mycobacterium* ; Nontuberculous Mycobacteria ; Skin ; Soft Tissue Infections ; Swimming Pools ; Tibia

Intracranial fibro-osseous lesion, also reported as calcifying pseudoneoplasm of the neural axis, is an uncommon lesion of the central nervous system. Since the discovery of this entity by Rhodes and Davis in 1978, there have been a total of 21 cases reported in the literature. We encountered one such case in a 28 year old male, who presented with left hemiparesis for 1 year. By the MR images, a 1.5 cm sized round mass was found at right parietal lobe near motor cortex. The mass lesion enhanced well, homogenously and revealed clear, slightly irregular margin. Excisional biopsy of the mass was performed. Microscopically the lesion was composed of calcified fibrous tissue with an amorphous gray-blue, coarsely fibrillar to chondromyxoid nodular areas. Sparse spindle cells, immunohistochemically negative for GFAP, vimentin and S-100, were scattered within the amorphous material. Palisading spindle or polygonal cells were present at the more cellular periphery of the lesion, which were vimentin positive but S-100 negative. There was no evidence of the pilocytic astrocytes, Rosenthal fibers, or GFAP positive hypertrophic astrocytes. Intracranial fibro-osseous lesions are apparently slow-growing with generally excellent prognosis after wide excision. The etiology remains unclear, but most investigators favor a reactive rather than neoplastic process.
Adult ; Astrocytes ; Axis, Cervical Vertebra ; Biopsy ; Central Nervous System ; Humans ; Male ; Motor Cortex ; Paresis ; Parietal Lobe ; Prognosis ; Research Personnel ; Vimentin

Diagnosis of adenocarcinoma of the prostate based purely on digital rectal examination will be correct in only 70% of cases. Transitional cell carcinoma also arises in the prostate, as may more rarely sarcoma. The fibrous prostate, granulomatous prostatitis and stone may also result in a prostate that feels firmer than normal. Estrogen or orchiectomy will be of no benefit in these condition and may cause harm. Histological or cytological proof of the diagnosis is essential. Examination of prostatic tissue obtained by biopsy has been used to confirm malignancy or any type, judge the response of a tumor to either hormonal or cytotoxic manipulation and bacteriologic confirmation. The transrectal prostatic biopsy was performed on 48 cases of oriental, from May 1979 to February 1982. The results are as follows. 1. The patients ranged in age from 25 to 82 years old and the percentage of patients more than 50 years old is 85.4% (38 cases). 2. The highest reason of referral for prostatic biopsy is obstructive symptoms (62.5% - 30 cases). 3. As shown in table 3, only in 2 cases the specimens obtained with Silverman needle proved inadequate for a reliable diagnosis even though in 46 cases the specimens were thought to be adequate. In 46 cases, the highest disease is B.P.H. (73.9% - 34 cases), 2nd prostatic adenocarcinoma (17.5% - 8 cases), 3fd chronic prostatitis (4.3% - 2 cases) and normal prostate (4.3% - 2 cases). 4. There were 7 complications of transrectal prostatic biopsy and the hematuria (4 cases) was the most common.
Adenocarcinoma ; Aged, 80 and over ; Biopsy* ; Carcinoma, Transitional Cell ; Diagnosis ; Digital Rectal Examination ; Estrogens ; Hematuria ; Humans ; Middle Aged ; Needles ; Orchiectomy ; Prostate ; Prostatitis ; Referral and Consultation ; Sarcoma