No abstract available.
Adult* ; Humans ; Purpura, Thrombocytopenic, Idiopathic*

No abstract available.
Adult* ; Humans ; Purpura, Thrombocytopenic, Idiopathic*

Peripheral blood stem cells(PBSC) were collected for autologous transplantation for the first time in Korea wish Fenwal CS-3000 Plus blood cell separator. Twenty-nine procedures were performed for 7 patients without any incidents. GM-CSF(400ug) was administered daily for 5 days before PBSC collection and the collections were performed 3-6 successive days. A total of 3.5-11.4 x 109 mononuclear cells(MNCs) were collected from 7,000mL blood processed per procedure. The number of MNCs collected was mainly dependent on the concentration of MNCs in patient's blood(r = 0.68, p<0.01). We concluded that the PBSC trans plantation program could be set up without difficulty in Korea since the Fenwal CS-3000 Plus blood cell separator is already available in many institutions and the procedure used in this study is easy to follow.
Autografts ; Blood Cells* ; Humans ; Korea ; Stem Cells* ; Transplantation, Autologous

No abstract available.
Blood Platelets* ; Blood Transfusion*

Three Listeria monocytogenes strains isolated from clinical cerebrospinal fluid samples. A PCR was performed by using oligonuclotide primers derived from the invasion-associated protein(iap) related gene sequence. As a result of PCR, we obtained 287-bp DNA fragment of specific product to L. monocytogenes species. The chromsomal DNA of genera Listeria species were subjected to southern blot hybridization with HRP-labeled 287-bp DNA probe to produce a L. monocytogenes banding pattern. In the cases of 10-fold dilution of L. monocytogenes cells, 8 x 10(2) cells/ml were detected by PCR.
Blotting, Southern ; Cerebrospinal Fluid ; DNA ; Listeria monocytogenes* ; Listeria* ; Polymerase Chain Reaction*

No abstract available.
Classification* ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive*

No abstract available.
Cytarabine* ; Cytosine* ; Drug Therapy, Combination* ; Idarubicin* ; Leukemia, Myeloid, Acute*

PURPOSE: Peripheral T-cell lymphomas(PTCL) show diverse clinical and histological characteristics and should be understood as mixtures of heterogeneous entities. Although many clinical and biological parameters have been proposed for classifying PTCL into different prognostic groups, few parameters have turned out to be appropriate for classification. To investigate the clinical significance of FUO presentation in PTCL, comparisons of clinical parameters were performed using non-FUO presentation as a control. MATERIALS AND METHODS: 66 cases of Korean PTCL were divided into FUO group and non-FUO group according to the presentation and compared with each other. RESULTS: Among 66 patients of PTCL, 19 patients presented with FUO. Compared with non-FUO group, FUO group showed no significant age and sex ratio differences. FUO group showed more advanced stage, worse performance status than non-FUO group. Predominant sites of definite diagnosis were skin, gastrointestinal tract and liver in FUO group and nasal cavity and paranasal sinus in non-FUO group. There were no significant differences between histologic classifications of both groups. Survival analysis revealed significant differences between both groups. FUO group showed significantly shorter survival. Prognostic factor analysis(multivariate) was done with stage, LDH level, performance status, and FUO status. FUO status, stage and performance status were significant determinants of survival, but LDH level proved to have no prognostic implication. CONCLUSION: PTCL with FUO presentation showed such distinct characteristics that the authors propose fever of unknown origin(FUO) as a clinical parameter for classifying PTCL. Further studies are needed to identify biological parameters which characterize PTCL with FUO presentation.
Classification ; Diagnosis ; Fever of Unknown Origin* ; Fever* ; Gastrointestinal Tract ; Humans ; Liver ; Lymphoma* ; Nasal Cavity ; Prognosis ; Sex Ratio ; Skin ; T-Lymphocytes

No abstract available.
Drug Therapy, Combination* ; Melphalan* ; Multiple Myeloma* ; Prednisone* ; Vincristine*

BACKGROUND: Flow cytometric measurement of DNA can reveal G0/G1, S, G2/M phases of cell cycle, and BrdU labeling can determine the percentage of cells in active DNA synthesis. A monoclonal antibody (MoAb), Ki-67, recognizes a protein that is present only in the nucleus of cycling cells but absent in resting cells. We analyzed whether the resting and the proliferating fraction could be differentiated by double staining with Ki-67 MoAb and propidium iodide (PI), and observed the effects of GM-CSF on cell cycle in acute myelogenous leukemia (AML) cells by Ki-67 MoAb. METHODS: Blast cells were prepared from 9 AML patients. The cells were incubated for 48 hours with or without GM-CSF. Cells were stained with BrdU/PI and Ki-67/PI. Cell cycle was analyzed by flow cytometry. RESULTS: The average fraction of G0/G1, S, and G2/M phases was 84.6%, 10.9%, and 4.5 % by BrdU/PI and 87.8%, 8.6%, and 3.7% by Ki-67/PI, respectively. Ki-67/PI staining dis-criminated between G0 and G1 phases and the average was 71.5% and 16.3%, respectively. In cells incubated with GM-CSF, BrdU/ PI method showed that the average S phase fraction (SPF) significantly increased from 10.9 to 16.2% (P=0.01) and the fraction of G0/G1 phase decreased from 84.6% to 78.4% (P= .02). Ki-67/PI method showed that the median SPF significantly increased from 8.6% to 13.7% (P=0.05) and G0 fraction decreased from 71.5% to 58.1% (P=0.02) but G1 fraction increased from 16.3% to 22.3% (P=0.01). CONCLUSION: Cell cycle analysis by Ki-67 MoAb and PI in AML is rapid and simple. It is especially useful to determine the growth fraction and G0 fraction compared to BrdU/PI staining.
Bromodeoxyuridine ; Cell Cycle* ; DNA ; Flow Cytometry ; G0 Phase ; G1 Phase ; Granulocyte-Macrophage Colony-Stimulating Factor ; Humans ; Ki-67 Antigen ; Leukemia, Myeloid, Acute* ; Propidium ; S Phase