1.Paraquat toxicity in pregnant mice and their fetuses.
Korean Journal of Obstetrics and Gynecology 1992;35(10):1448-1456
2.Superoxide Dismutase, Catalase and Glutathione Peroxidase Activities in Erythrocytes and Synovial Fluid of the Osteoarthritis of the Knee Joint
Dong Min SHIN ; Young Rae MOON ; Byoung Rai LEE
The Journal of the Korean Orthopaedic Association 1994;29(1):44-49
Superoxide dismutase(SOD), catalase and glutathione peroxidase and the levels of lipid peroxide were assayed in erythrocytes and synovial fluid isolated from 17 patients with osteoarthritis of the knee joints and 7 with healthy knee joints as a control groups. In the erythrocytes, SOD, catalase and glutathione peroxidase activities were significantly increased in osteoarthritis compared with normal control groups, but the changes of malonyldialdehyde level was not significant. The activity of SOD in synovial fluid was significantly decreased in osteoarthritis compared with normal control groups, but catalase activity was significantly increased in synovial fluid of osteoarthritis. This result suggested that the increament of antioxidant enzymes in erythrocytes were probably due to increased production of oxygen radicals in osteoarthritis. In osteoarthritis, knee joints might be injured more easily by oxygen radicals because of decreased activity of SOD in synovial fluid of osteoarthritis.
Catalase
;
Erythrocytes
;
Glutathione Peroxidase
;
Glutathione
;
Humans
;
Joints
;
Knee Joint
;
Knee
;
Malondialdehyde
;
Osteoarthritis
;
Osteoarthritis, Knee
;
Reactive Oxygen Species
;
Superoxide Dismutase
;
Superoxides
;
Synovial Fluid
3.A Case of Laurence-Moon-Biedl Syndrome Including Diabetic Mellitus.
Byoung Hoon LEE ; Byung Rai CHO ; Myoung Ik LEE ; Keun Chan SOHN ; Hyung Joon YOO
Journal of the Korean Pediatric Society 1989;32(6):857-861
No abstract available.
Laurence-Moon Syndrome*
4.A Case of Laurence-Moon-Biedl Syndrome Including Diabetic Mellitus.
Byoung Hoon LEE ; Byung Rai CHO ; Myoung Ik LEE ; Keun Chan SOHN ; Hyung Joon YOO
Journal of the Korean Pediatric Society 1989;32(6):857-861
No abstract available.
Laurence-Moon Syndrome*
5.Changes of Nitric Oxide Synthase (NOS) Isozymes in Cultured Human Colon Carcinoma Cell.
Jin Young PARK ; Cheong Yong KIM ; Byoung Rai LEE
Journal of the Korean Society of Coloproctology 2000;16(4):209-214
Nitric oxide (NO), the production of which is dependent on Nitric oxide synthase (NOS), has been shown to contribute to pathogeneses in various diseases. Recent investigations of NOS expression in tumor tissues indicate that NO may mediate one or more roles during the growth of human cancers. The aim of this study was to determine whether iNOS is expressed in human colon carcinoma cell lines and to determine the types of NOS isozymes in colon carcinoma cell lines with high and low metastatic potentials. METHODS: We measured the expressions of iNOS and eNOS and the formation of nitrotyrosine which indicates peroxinitrate production in highly metastatic colon cancer cell (KM1214) and lowly metastatic colon cancer cell (KM12C) by Western blots. RESULTS: The iNOS were detected in both KM1214 and KM12C by Western blot analysis. The expression of iNOS in KM1214 cells was significantly higher than in KM12C cells. The expression of iNOS was increased with lipopolysaccharide (LPS) in colon cancer cells but the rate of increase was higher in KM1214 cells than in KM12C cells. CONCLUSIONS: In human colon carcinoma cells, iNOS is expressed in cancer cells and expression of iNOS is higher in highly metastatic colon cancer cells than in lowly metastatic colon cancer cells and iNOS expression may have some role in colon cancer metastasis.
Blotting, Western
;
Cell Line
;
Colon*
;
Colonic Neoplasms
;
Humans*
;
Isoenzymes*
;
Neoplasm Metastasis
;
Nitric Oxide Synthase*
;
Nitric Oxide*
6.Effects of Glutathione on Reactive Oxygen Species-Induced Cytotoxicity in Human Retinal Pigment Epithelial Cell Line.
Jae Woong KOH ; Byoung Rai LEE ; Nam Chul JI
Journal of the Korean Ophthalmological Society 2002;43(9):1774-1780
PURPOSE: Oxidative stress is the well known causative factor for retinal damage. This study investigated the effects of glutathione on reactive oxygen species(ROS) induced injury in human retinal pigment epithelial(HRPE) cells. OBJECTS AND METHODS: HRPE cells (ATCC:CRL-2302) were cultured with DMEM media and exposed to oxidative stress (paraquat, hydrogen peroxide) and/or glutathione modulator[(buthionine sulfoximine (BSO), glutathione (GSH), 2-oxo 4-thiazolidine carboxylic acid (OTC)] for 2 days. The cell viability was determined by measuring the amount of reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT). RESULTS: The rate of MTT reduction of HRPE cells decreased by either paraquat or hydrogen peroxide treatment. BSO as a inhibitor of glutathione biosynthesis potentiated paraquat- or hydrogen peroxideinduced HRPE cells injury. On the other hand GSH or OTC reduced the rate of decrement of MTT reduction in HRPE cells by paraquat and hydrogen peroxide. CONCLUSIONS: Glutathione seemed to play some role in prevention of ROS-induced HRPE cells injury and OTC may be used as an agents for prevention of free radical induced HRPE cell injury.
Cell Survival
;
Epithelial Cells*
;
Glutathione*
;
Hand
;
Humans*
;
Hydrogen
;
Hydrogen Peroxide
;
Oxidative Stress
;
Oxygen*
;
Paraquat
;
Retinaldehyde*
7.The effect of pertussis vaccine on streptozotocin induced diabetic rats.
Kyung Rae MOON ; Kwang Rhun KOO ; Sang Kee PARK ; Yeong Bong PARK ; Byoung Rai LEE ; Keun Hong KEE
Journal of the Korean Pediatric Society 1991;34(7):897-906
No abstract available.
Animals
;
Pertussis Vaccine*
;
Rats*
;
Streptozocin*
;
Whooping Cough*
8.The Effects of High Glucose on Paraquat-induced Cell Injury in Human Retinal Pigment Epithelial Cells.
Jae Woong KOH ; Byoung Rai LEE ; Gwang Ju CHOI
Journal of the Korean Ophthalmological Society 2002;43(5):883-889
PURPOSE: Functional and structural alterations of retinal pigment epithelial cells were observed in experimental and clinical diabetes. To investigate the effects of high glucose on free radical-induced injury in retinal pigment epithelial cells, we determined the effects of high glucose on activities of antioxidant enzymes and paraquat-induced cytotoxicity in cultured human retinal pigment epithelial (HRPE) cells. METHODS: Human retinal pigment epithelial (HRPE) cell line (ATCC:CRL-2302) was cultured with high glucose (22.4 mM)-and normoglucose (5.6 mM)-contained DMEM for 3 days. The activities of superoxide dismutase (SOD), catalase and GSHPx were assayed by Crapo's method, Aebi's method and GUnzler's method, respectively. Paraquat-induced cytotoxicity was determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) method. RESULTS: CuZn-SOD activity of HRPE cells was decreased by 32% in high glucose (25.4 mM) media compared to normoglucose (5.6 mM) media. But the activities of catalase and GSHPx were not changed by high glucose. The paraquat-induced HRPE cells toxicity was increased by high glucose. Diethydithiocarbamate (DDC), as inhibitor of CuZn-SOD, also potentiated paraquat-induced HRPE cell CONCLUSIONS: Paraquat-induced HEPE cells injury was potentiated by high glucose and decreased CuZn-SOD activity by high glucose may be some roles in free radical-induced HRPE cell injury.
Catalase
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Cell Line
;
Epithelial Cells*
;
Glucose*
;
Humans*
;
Paraquat
;
Retinaldehyde*
;
Superoxide Dismutase
9.The Protective Effects of Green Tea Extract against L-arginine Toxicity to Cultured Human Mesangial Cells.
Byung Chul SHIN ; Hyun Ho RYU ; Jong Hoon CHUNG ; Byoung Rai LEE ; Hyun Lee KIM
Journal of Korean Medical Science 2009;24(Suppl 1):S204-S209
The aim of this study was to investigate whether green tea extract (GTE) has the protective effects on excess L-arginine induced toxicity in human mesangial cell. Human mesangial cells treated with L-arginine were cultured on Dulbecco's modified eagle medium in the presence and absence of inducible nitric oxide synthase (iNOS) inhibitor and GTE. The cell proliferation was determined by 3 (4,5-dimethylthiazol- 2-yl)-2, 5-diphengltetrqzolium bromide, a tetrazole assay. The iNOS mRNA and its protein expression were detected by reverse transcription polymerase chain reaction and Western blot, respectively. The concentration of nitric oxide (NO) was measured by NO enzyme-linced immuno sorbent assay kit. L-arginine significantly inhibited the proliferation of human mesangial cells, and induced the secretion of NO to the media. NO production by L-arginine was significantly suppressed by GTE and iNOS inhibitor (p<0.01). The expression level of iNOS mRNA and its protein that was significantly increased by L-arginine was decreased by iNOS inhibitor but not by GTE. GTE protected the mesangial cells from the NO-mediated cytotoxicity by scavenging the NO rather than by iNOS gene expression. Therefore, we conclude that GTE has some protective effect for renal cells against oxidative injury possibly by polyphenols contained in GTE.
Antioxidants/metabolism
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Arginine/metabolism/pharmacology/*toxicity
;
Cell Line
;
Cell Proliferation
;
Cell Survival
;
Flavonoids/metabolism
;
Glomerular Mesangium/cytology/metabolism
;
Humans
;
Mesangial Cells/*cytology/metabolism
;
Nitric Oxide/chemistry/metabolism
;
Nitric Oxide Synthase Type II/metabolism
;
Phenols/metabolism
;
RNA, Messenger/metabolism
;
Reverse Transcriptase Polymerase Chain Reaction
;
Tea
10.The Changes of Superoxide Dismutase Isozymes in the Placenta of Patients with Preeclampsia.
Tae Gyu AHN ; Hyuk JUNG ; Chang Hun SONG ; Sang Joon CHOI ; Byoung Rai LEE ; Sei Jun HAN
Korean Journal of Obstetrics and Gynecology 2003;46(6):1104-1109
OBJECTIVE: The aim of this article was investigated whether changes of superoxide dismutase isozymes in the placenta of patients with preeclampsia contribute to radical-induced tissue injury. METHODS: The activities of antioxidant enzymes (superoxide dismutase (SOD), catalase, glutathione peroxidase (GSHPx)) and the contents of thiobarbituric acid reactive substance (TBARS) in the erythrocytes and in the placenta were assayed from 35 women with preclampsia and 35 normotensive pregnant women. RESULTS: The superoxide dismutase (SOD) activities were significantly reduced in the erhtyrocytes and the placenta of patients with pre-eclampsia compared with normotensive pregnant women. The activity of catalase was increased in the erythrocytes of patients with preeclampsia but the statistically significant difference of catalase activity in the placenta and GSHPx activity in both erythrocytes and placenta were not observed. The contents of TBARS were increased significantly in the erythrocytes and placenta of patients with preeclampsia. In preeclamptic placenta, copper and zinc containing superoxide dismutase (CuZn-SOD) was decreased (3.9+/-0.5 vs 5.1+/-0.6 U/mg protein) whereas manganeus containing superoxide dismutase (Mn-SOD) was increased (2.0+/-0.3 vs 2.7+/-0.4 U/mg protein). CONCLUSION: In these results, the decreased CuZn-SOD activity may some roles in increment of TBARS contents in pre-eclamptic placenta and decreased CuZn-SOD activity may be more prone to oxidative stress in the placenta.
Catalase
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Copper
;
Erythrocytes
;
Female
;
Glutathione Peroxidase
;
Humans
;
Isoenzymes*
;
Oxidative Stress
;
Placenta*
;
Pre-Eclampsia*
;
Pregnant Women
;
Superoxide Dismutase*
;
Superoxides*
;
Thiobarbituric Acid Reactive Substances
;
Zinc