1.Early results of the sternocostal elecation for pectus excavatum.
The Korean Journal of Thoracic and Cardiovascular Surgery 1992;25(6):621-629
No abstract available.
Funnel Chest*
2.An evaluation of the thoracotomy in spontaneous pneumothorax.
Byoung Hee AHN ; Won Chae HANG
The Korean Journal of Thoracic and Cardiovascular Surgery 1993;26(5):390-394
No abstract available.
Pneumothorax*
;
Thoracotomy*
3.Follow up Study of Outcome in Severe Hyperbilirubinemic Newborns Treated with Exchange Transfusion and Phototherapy.
Byoung Sun AHN ; Hyun Min PARK ; Baeck Hee LEE
Journal of the Korean Child Neurology Society 1999;7(1):96-106
PURPOSE: Cerebral palsy or hearing disability of hyperbilirubinemic complication was reduced by blood exchange transfusion(BET) and phototherapy(PT). But in spite of these treatment, abnormal Auditory Brainstem evoked Response(ABR) finding after BET or PT and neurodevelopmental defect due to chronic bilirubin encephalopathy were observed. So we have studied risk factors and outcome of chronic bilirubin encephalopathy after BET, and treatment of hyperbilirubinemia. METHODS: We have analyzed clinical characteristics, the finding and change of ABR after BET in 17 hyperbilirubinemic neonates, and in 8 hyperbilirubinemic neonates who were treated by phototherapy and 15 normal control neonates. RESULTS: 1) Mean bilirubin concentraion were 27.5+/-4.1mg/dL in BET group and 22.1+/-2.3 mg/dL in PT group. There were no difference of clinical findings between BET and PT group. 2) Change of ABR (1) Wave I loss resulted in 4 neonates, wave III loss in 3 neonates, and wave V loss in 2 neonates in BET group(P<0.05). (2) Wave I peak latency and hearing threshold in BET group were significantly increased more than normal control group(P<0.01). 3) In 10 neonates(58.8%) among 17 BET group, 4 neonates(50%) in 8 PT group were observed abnormal initial ABR finding after jaundice treatment. Age at treatment and duration of jaundice(interval between onset of jaundice and treatment) in abnormal ABR group were significant prolongation compared with normal ABR group(P<0.05). 4) Chronic bilirubin encephalopathy(CBE) was observed in 3 neonates(17.6%) among 17 BET group and showed higher of bilirubin level than normalized group after BET (31.1mg/dL vs 26.6mg/dL), other clinical findings showed no significant differences. CONCLUSION: Bilirubin level was significantly elevated in CBE more than in BET group and duration of jaundice, age at treatment were longer in abnormal ABR group than in normal ABR group. So not only bilirubin level but also duration of jaundice shoud be considered at jaundice treatment, and ABR has a potential utility in detection of acute brain toxicity of bilirubin and follow up evaluation of bilirubin encephalopathy.
Bilirubin
;
Brain
;
Brain Stem
;
Cerebral Palsy
;
Follow-Up Studies*
;
Hearing
;
Humans
;
Hyperbilirubinemia
;
Infant, Newborn*
;
Jaundice
;
Kernicterus
;
Phototherapy*
;
Risk Factors
4.Electrophysiological Effects of Purinergic Receptor Agonists on Atrial Muscle Fiber under Normal and Ischemic Conditions.
Jae Ha KIM ; Byoung Hee AHN ; Jung Chaee KANG
Korean Circulation Journal 1994;24(4):645-654
BACKGROUND: The electrophysiological effects of purinergic receptor agonists, adenosine triphosphate(ATP) and adenosine were examined using conventional microelectrode technique in rat atrial muscle fibers under superfused with a normal or a simulated ischemic(hypoxic, hyperkalemic and acidotic) physiologic salt solution(PSS) in vitro. METHODS: Action potential parameters, such as maximal diastolic potential(MDP), action potential amplitude(APA), rate of phase 0 depolarization(dv/dtmax) and action potential duration(APD90) were measured in electrically paced, physiologic salt solution(Tyrode's) superfused left rat atrium. In the experiment of ischemic simulation in vitro, normal physiologic salt solutions(NPSS0 were modified(MPSS) and superfused in substitute for normal Tyrode's solution. To investigate the effects of purinergic receptor agonists, ATP or adenosine was added to the superfused tyrode's solutions(NPSS or MPSS) in molar concentration. RESULTS: Under superfused with normal PSS, ATP(10(-3), 10(-4)M) elicited slight hyperpolarization in MDP, and both ATP(10(-6)-10(-3)M) and adenosine(10(-6)-10(-3)M) shortened the duration of normal action potential in a dose-dependent manner. The other paramaters were not affected by the drugs. Superfusion with ischemic PSS caused reductions in MDP as well as APA, dv/dtmax and, especially, APD90. The effects produced by the initial 10 minutes of superfusion with ischemic PSS almost completely disappeared during a subsequent period of continued superfusion with normal PSS, but, those by the initial 20 min lasted in some degree. Both ATP(10(-4)M) and adenosine(10(-4)M) attenuated the reduction in the rate of phase 0 depolarization and the amplitude of the action potential amplitude produced by the ischemic PSS. CONCLUSION: Purinergic receptor agonists, ATP and adensoine, caused a concentration-dependent shortening of the action potential duration in rat atrial muscle fibers and they attenuated the reductions in the rate of phase 0 depolarization and action potential amplitude in fibers superfused with ischemic PSS.
Action Potentials
;
Adenosine
;
Adenosine Triphosphate
;
Animals
;
Ischemia
;
Microelectrodes
;
Molar
;
Purinergic Agonists*
;
Rats
;
Receptors, Purinergic
5.Analysis of Histological Findings and Estrogen and Progesterone Receptor Status in Luteal Phase Defect Caused by Delayed Ovulation and Clomiphen Treatment.
Kyu Rae KIM ; Hee Jeong AHN ; Jai Hyang GO ; Dong Hee CHOI ; Byoung Goo YOON
Korean Journal of Pathology 1996;30(12):1106-1115
Luteal phase defect (LPD) is an ovulatory disorder of considerable clinical importance that leads to delayed endometrial maturation and inadequate endometrium for blastocyst implantation. This disorder is implicated in infertility and recurrent spontaneous abortion. We analyzed the endometrial histological maturation, and the estrogen receptor(ER) and progesterone receptor(PR) status in patients with unexplained delayed ovulation, and in patients with ovulation induction by clomiphen citrate. The purpose of this study was to determine whether the length of the follicular phase influences the endometrial histological maturation and the hormonal receptor status, and to know the effect of clomiphen citrate on the endometrium. In the delayed ovulation group, the endometrium was characterized by an irregular outpouching and dilated lumina of the glands, a decreased secretory activity and predecidualization, and a decreased number of granulocytes in the stroma. In the clomiphen citrate-ovulation induction group, glandular proliferation was markedly diminished with poorly convoluted and narrow glands, secretory activity was decreased, stroma was undecidualized, and there was an absence of granulocytes. ER expression was increased in the glandular epithelium in the delayed ovulation group, and both ER and PR expressions were markedly decreased in the glandular epithelium and stromal cells in the ovulation induction group. Endometrial maturation and differentiation may be diminished by increased estrogenic and relatively deficient progesterone effects in patients with delayed ovulation. The hormonal receptor status might also be influenced by hormonal changes. Clomiphen citrate successfully induced ovulation in patients with delayed ovulation, however, endometrial proliferation and maturation were markedly suppressed. This might be related to the lower pregnancy rate and higher abortion rate in patients with clomiphen citrate induced ovulation.
Pregnancy
;
Female
;
Humans
6.Clinical Observation on Admitted Patient in Nursery.
Back Hee LEE ; Byoung Hoon LEE ; Don Hee AHN ; Keun Chan SOHN
Journal of the Korean Pediatric Society 1989;32(4):478-485
No abstract available.
Humans
;
Nurseries*
7.The Findings of 2D and M-mode Echocardiography in Young Insulin-Dependent Diabetes Mellitus.
Byoung Rei CHO ; Jae Wook KO ; Young Jin HONG ; Don Hee AHN ; Keun Chan SOHN
Journal of the Korean Pediatric Society 1990;33(1):88-93
No abstract available.
Diabetes Mellitus, Type 1*
;
Echocardiography*
8.A Case of Pierre Robin Syndrome.
Hyun Hwa KIM ; Hae Sook CHA ; Byoung Hai AHN ; Young Hee YOU ; Hyun Sook LEE
Journal of the Korean Pediatric Society 1984;27(5):488-492
No abstract available.
Pierre Robin Syndrome*
9.Serotyping and Phylogenetic analysis of Enteroviruses Isolated from Patients with Aspetic Meningitis.
Jung Hee LEE ; Byoung Yoon AHN ; Sung Hwan BAN ; Sang Hyun KIM ; Eui Chong KIM
Korean Journal of Clinical Microbiology 2000;3(2):121-131
BACKGROUND: The determination of serotype of enteroviruses is useful for the discrimination between sporadic and epidemic infections. The conventional serotyping method is time-consuming and labor-intensive. Recently, molecular method was introduced for the serotyping of enteroviruses. The aim of this study was to establish a method to isolate and analyze enteroviruses from various specimens utilizing molecular biological techniques and to determine which strains were phylogenetically related to clinical samples. METHODS: Clinical samples in this study included 164 cerebrospinal fluid (CSF), 136 stool, 15 sera, 6 throat swab, 5 urine, and 4 sputa, which were obtained from hospitalized patients, primarily infants or children presenting symptoms of aseptic meningitis in 1998. RD cells were used for enterovirus isolation. RT-PCR was performed with RD cell lysate showing CPE. The primers 011 and 012 were used for the VP1 region, and the primers EN1 and EN2 for 5'-UTR. The nucleotide sequences of VP1 region were determined and analyzed with BLAST program. RESULTS: Among 333 samples, only 23 samples produced CPE: 17 samples at first and six samples at the second blind passage. Fifteen isolates were related to coxsackievirus B2 two to echovirus 4, three to echovirus 6, and three to echovirus 18. All 23 viral isolates displayed a nucleotide sequence identity of 80-95%, compared with the reference serotypes. However, the identity was increased up to 93-100% when the VP1 region was translated into amino acids CONCLUSIONS: Since CB2 type was 55% among enteroviral isolates, the CB2 was determined as the major causative serotype of enteroviral meningitis in 1998. CB2 type was emerged between June and July, EC4 and EC6 was limited to July, and EC18 was in August.
Amino Acids
;
Base Sequence
;
Cerebrospinal Fluid
;
Child
;
Discrimination (Psychology)
;
Echovirus 6, Human
;
Enterovirus B, Human
;
Enterovirus*
;
Humans
;
Infant
;
Meningitis*
;
Meningitis, Aseptic
;
Pharynx
;
Serotyping*
10.Serotyping and Phylogenetic analysis of Enteroviruses Isolated from Patients with Aspetic Meningitis.
Jung Hee LEE ; Byoung Yoon AHN ; Sung Hwan BAN ; Sang Hyun KIM ; Eui Chong KIM
Korean Journal of Clinical Microbiology 2000;3(2):121-131
BACKGROUND: The determination of serotype of enteroviruses is useful for the discrimination between sporadic and epidemic infections. The conventional serotyping method is time-consuming and labor-intensive. Recently, molecular method was introduced for the serotyping of enteroviruses. The aim of this study was to establish a method to isolate and analyze enteroviruses from various specimens utilizing molecular biological techniques and to determine which strains were phylogenetically related to clinical samples. METHODS: Clinical samples in this study included 164 cerebrospinal fluid (CSF), 136 stool, 15 sera, 6 throat swab, 5 urine, and 4 sputa, which were obtained from hospitalized patients, primarily infants or children presenting symptoms of aseptic meningitis in 1998. RD cells were used for enterovirus isolation. RT-PCR was performed with RD cell lysate showing CPE. The primers 011 and 012 were used for the VP1 region, and the primers EN1 and EN2 for 5'-UTR. The nucleotide sequences of VP1 region were determined and analyzed with BLAST program. RESULTS: Among 333 samples, only 23 samples produced CPE: 17 samples at first and six samples at the second blind passage. Fifteen isolates were related to coxsackievirus B2 two to echovirus 4, three to echovirus 6, and three to echovirus 18. All 23 viral isolates displayed a nucleotide sequence identity of 80-95%, compared with the reference serotypes. However, the identity was increased up to 93-100% when the VP1 region was translated into amino acids CONCLUSIONS: Since CB2 type was 55% among enteroviral isolates, the CB2 was determined as the major causative serotype of enteroviral meningitis in 1998. CB2 type was emerged between June and July, EC4 and EC6 was limited to July, and EC18 was in August.
Amino Acids
;
Base Sequence
;
Cerebrospinal Fluid
;
Child
;
Discrimination (Psychology)
;
Echovirus 6, Human
;
Enterovirus B, Human
;
Enterovirus*
;
Humans
;
Infant
;
Meningitis*
;
Meningitis, Aseptic
;
Pharynx
;
Serotyping*