1.Electrophysiological Effects of Purinergic Receptor Agonists on Atrial Muscle Fiber under Normal and Ischemic Conditions.
Jae Ha KIM ; Byoung Hee AHN ; Jung Chaee KANG
Korean Circulation Journal 1994;24(4):645-654
BACKGROUND: The electrophysiological effects of purinergic receptor agonists, adenosine triphosphate(ATP) and adenosine were examined using conventional microelectrode technique in rat atrial muscle fibers under superfused with a normal or a simulated ischemic(hypoxic, hyperkalemic and acidotic) physiologic salt solution(PSS) in vitro. METHODS: Action potential parameters, such as maximal diastolic potential(MDP), action potential amplitude(APA), rate of phase 0 depolarization(dv/dtmax) and action potential duration(APD90) were measured in electrically paced, physiologic salt solution(Tyrode's) superfused left rat atrium. In the experiment of ischemic simulation in vitro, normal physiologic salt solutions(NPSS0 were modified(MPSS) and superfused in substitute for normal Tyrode's solution. To investigate the effects of purinergic receptor agonists, ATP or adenosine was added to the superfused tyrode's solutions(NPSS or MPSS) in molar concentration. RESULTS: Under superfused with normal PSS, ATP(10(-3), 10(-4)M) elicited slight hyperpolarization in MDP, and both ATP(10(-6)-10(-3)M) and adenosine(10(-6)-10(-3)M) shortened the duration of normal action potential in a dose-dependent manner. The other paramaters were not affected by the drugs. Superfusion with ischemic PSS caused reductions in MDP as well as APA, dv/dtmax and, especially, APD90. The effects produced by the initial 10 minutes of superfusion with ischemic PSS almost completely disappeared during a subsequent period of continued superfusion with normal PSS, but, those by the initial 20 min lasted in some degree. Both ATP(10(-4)M) and adenosine(10(-4)M) attenuated the reduction in the rate of phase 0 depolarization and the amplitude of the action potential amplitude produced by the ischemic PSS. CONCLUSION: Purinergic receptor agonists, ATP and adensoine, caused a concentration-dependent shortening of the action potential duration in rat atrial muscle fibers and they attenuated the reductions in the rate of phase 0 depolarization and action potential amplitude in fibers superfused with ischemic PSS.
Action Potentials
;
Adenosine
;
Adenosine Triphosphate
;
Animals
;
Ischemia
;
Microelectrodes
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Molar
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Purinergic Agonists*
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Rats
;
Receptors, Purinergic
2.Echocardiographic Evaluation of Sequential Change of Cardiac Function in Normal Neonates.
Ji In PARK ; Chul Ha KIM ; Byoung Hoon YOO
Journal of the Korean Pediatric Society 1990;33(11):1533-1539
No abstract available.
Echocardiography*
;
Humans
;
Infant, Newborn*
3.A Case of Congenital Hypofibrinogenemia.
Ji In PARK ; In Seok LIM ; Chul Ha KIM ; Byoung Hoon YOO
Journal of the Korean Pediatric Society 1990;33(7):1009-1011
No abstract available.
4.Renovascular Hypertension in Children.
Byoung Chul KANG ; Il Soo HA ; In One KIM ; Hae Il CHEONG ; Yong CHOI ; Kwang Wook KO
Journal of the Korean Society of Pediatric Nephrology 1997;1(2):101-108
The application of fine needle aspiration (FNA) cytology to the soft tissue tumors had been neglected. In recent years, however, FNA has been used increasingly in the preoperative diagnosis of these tumors due to its usefulness and accuracy. We present 3 cases of liposarcoma, myxoid, myxoid with round cell, and pleomorphic, diagnosed by FNA cytology with histologic confirmation. Good correlation between his- tologic and FNA cytologic findings was found. Although the cytologic appearances of liposarcomas varied with histologic type, the main criterion was the presence of atypical multivacuolated lipoblast with characteristically scalloped nuclei.
Biopsy, Fine-Needle
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Child*
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Diagnosis
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Humans
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Hypertension, Renovascular*
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Liposarcoma
;
Liposarcoma, Myxoid
;
Pectinidae
5.A Case of Idiopathic Long QT Syndrome with 2:1 Atrioventricular Block.
Kwang In LEE ; Chul Ha KIM ; In Seok LIM ; Dong Keun LEE ; Byoung Hoon YOO
Journal of the Korean Pediatric Society 1995;38(12):1701-1705
No abstract available.
Atrioventricular Block*
;
Long QT Syndrome*
6.Clinical Analysis of Serum and Urine N-Acetyl-B-D-Glucosaminidase(NAG) in Renal Disease.
Yo Han CHUNG ; In Seok LIM ; Chul Ha KIM ; Byoung Hoon YOO ; Tae Sub SHIM
Journal of the Korean Pediatric Society 1994;37(3):383-389
Recently, NAG activity has gained increasing importance as and aid in the diagnosis of renoparenchymal diseases. Elevation of urine NAG activity has been found to be an indicator of renoparenchymnal diseases. To evaluate the diagnostic value of the NAG activity test in the renal disease, we carried out clinical study on 31 cases of renal disease patients who had been admitted to the Department of Pediatrics, Chung-Ang University Hospital between March 1992 and February 1993. We analyese by two data: (1) Stastical Package for the Social Science. (2) Students'T test. The results were as follows 1) The urine NAG activity significantly increased (p<0.05) to 69.9+/-5165.69U/hr/mg Creatinine (U/hr/mg Cr) in the renal disease group compared to 3.6+/-1.91U/hr/mg Cr in the control group. 2) The serum NAG activity was 11.69+/-5.18U/L in the renal disease group and 10.58+/-4.04U/L in the control group. There was no significant difference in the serum NAG sctivity between two groups. 3) In the renal disease group, the serum NAG activity was 10.78+/-3.32U/L in male 12.53+/-6.47U/L in female. There was no significant difference between both sexes. In the renal disease, the urine NAG activity was 33.62+/-30.67U/hr/mg Cr in male and 114.05+/-241.62U/hr/mg Cr female. There was no significant difference between both sexes (p<0.05). 4) In the renal disease group, the urine NAG activity increased 314.73+/-420.39U/hr/mg Cr in the 2 years old group but there was no significant difference of urine NAG activity compared to above 2 years old group . 5) The urine NAG activities were 93.1+/-0193.04U/hr/mg Cr in the poteinuria subgroup and 13.3+/-47.62U/hr/mg Cr in the nonproteinuria subgroup and 3.66+/-1.91U/hr/mg Cr in the control group. There was no significant difference between the proteinuria subgroup and the nonproteinuria subgroup. but there was significant difference between nonproteinuria subgroup and control grouop(p<0.005).
Child, Preschool
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Creatinine
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Diagnosis
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Female
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Humans
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Male
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Pediatrics
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Proteinuria
;
Social Sciences
7.A Case of Thyroid Papillary Cancer Derived from Diffuse Goiter in a Patients with Acromegaly
Dong Hee KIM ; Jick Hwa NAM ; Byoung Ho SIN ; Ye Kyung SEO ; Jung Guk KIM ; Sung Woo HA ; Bo Wan KIM ; Young Ha LEE ; In Su SEO
Journal of Korean Society of Endocrinology 1996;11(3):311-317
Patients with acromegaly have a reduced life expectancy rnainly due to cardiovascular, respiratory or cerebrovascular diseas-. Malignancy also seems to occur with greater than the expected incidence. In particular, the published retrospective or prospective studies have suggested a strong association of colonic neoplasia with acromegaly. But, there were a few reports of thyroid cancer in acrornegaly. We report a case of thyroid papillary cancer derived from diffuse goiter in acromegaly, sugge- sting the possible carcinogenic role of growth hormone.
Acromegaly
;
Bites and Stings
;
Colon
;
Goiter
;
Growth Hormone
;
Humans
;
Incidence
;
Life Expectancy
;
Prospective Studies
;
Retrospective Studies
;
Thyroid Gland
;
Thyroid Neoplasms
8.BaSDAS: a web-based pooled CRISPR-Cas9 knockout screening data analysis system
Young-Kyu PARK ; Byoung-Ha YOON ; Seung-Jin PARK ; Byung Kwon KIM ; Seon-Young KIM
Genomics & Informatics 2020;18(4):e46-
We developed the BaSDAS (Barcode-Seq Data Analysis System), a GUI-based pooled knockout screening data analysis system, to facilitate the analysis of pooled knockout screen data easily and effectively by researchers with limited bioinformatics skills. The BaSDAS supports the analysis of various pooled screening libraries, including yeast, human, and mouse libraries, and provides many useful statistical and visualization functions with a user-friendly web interface for convenience. We expect that BaSDAS will be a useful tool for the analysis of genome-wide screening data and will support the development of novel drugs based on functional genomics information.
9.BaSDAS: a web-based pooled CRISPR-Cas9 knockout screening data analysis system
Young-Kyu PARK ; Byoung-Ha YOON ; Seung-Jin PARK ; Byung Kwon KIM ; Seon-Young KIM
Genomics & Informatics 2020;18(4):e46-
We developed the BaSDAS (Barcode-Seq Data Analysis System), a GUI-based pooled knockout screening data analysis system, to facilitate the analysis of pooled knockout screen data easily and effectively by researchers with limited bioinformatics skills. The BaSDAS supports the analysis of various pooled screening libraries, including yeast, human, and mouse libraries, and provides many useful statistical and visualization functions with a user-friendly web interface for convenience. We expect that BaSDAS will be a useful tool for the analysis of genome-wide screening data and will support the development of novel drugs based on functional genomics information.
10.A case of malignant atrophic papulosis (Degos' disease).
Sung Ku LEE ; In Seok LIM ; Chul Ha KIM ; Dong Keun LEE ; Eung Sang CHOI ; Byoung Hoon YOO ; Gae Yong SONG
Journal of the Korean Pediatric Society 1991;34(12):1724-1729
No abstract available.
Malignant Atrophic Papulosis*