No abstract available.
Progeria*

No abstract available.
Child* ; Humans ; Immunotherapy*

No abstract available.
Child* ; Goiter* ; Humans

No abstract available.
Periodicity* ; Skin*

No abstract available.
Congenital Hypothyroidism*

BACKGROUND AND OBJECTIVE: Buckwheat is considered one of the most important food allergens in Korea. Although a very small amount is ingested or inhaled, it can cause serious allergic reactions. However, the major allergens of buckwheat still remain to be elucidated. The aim of our study was to identify and characterize the major allergen of buckwheat seed. MATERIAL AND METHOD: Dialysis membrane with a cut-off MW 1kD was used for the preparation of crude buckwheat seed allergen extract. SDS-PAGE under reducing conditions and IgE immunoblotting were performed using sera from 15 buckwheat sensitive subjects. Isoelectric focusing and lectin blotting assay were done. RESULT: Western blot analysis showed more than 15 IgE-reactive buckwheat proteins. Among them, a 24kD protein was shown to be the most frequently bound to sera from allergic subjects (54%). Isoelectric point of 24kD protein was around 5.9. In lectin blotting assay, 24kD protein did not bind to Con A nor five other lectins. CONCLUSION: A 24kD protein was the most frequently recognized allergenic component in buckwheat seed. Isoelectric point was around 5.9. Glycosylation was not detected in 24kD of buckwheat protein.
Allergens ; Blotting, Western ; Dialysis ; Electrophoresis, Polyacrylamide Gel ; Fagopyrum* ; Glycosylation ; Hypersensitivity ; Immunoblotting ; Immunoglobulin E ; Isoelectric Focusing ; Isoelectric Point ; Korea ; Lectins ; Membranes

PURPOSE: Buckwheat is one of the most common food allegen in Korea and frequently elicit severe allergic reactions. However, up to now, only few reports on buckwheat allergens have been reproted. The purpose of this study was to isolate mRNA for the production of recombinant buckwheat allergens. METHODS: After the isolation of mRNA from ripening buckwheat seeds, in vitro translation was performed. The proteim patters of in vitro traslate products were identified using SDS-PAGE. RESULTS: A260/A260 ratio total RNA was 1.9 and mRNA was 2.0. In vitro translate products showed towered molecular buckwheat proteins such as 1, 3, and 14kD, while other high molecular weight protein of buckwheat seed were not shown in SDS-PAGE. CONCLUSION: mRNA of buckwheat was purified only from ripening seeds. The composition of mRNA was different according to the ripening periods. It is believed that this finding can give a clue to the basic research of buckwheat allergen.
Allergens ; Electrophoresis, Polyacrylamide Gel ; Fagopyrum* ; Hypersensitivity ; Korea ; Molecular Weight ; RNA ; RNA, Messenger

PURPOSE: Mold allergy is the one of the major causes of atopic diseases including asthma, and the Alternaria exists year-round in Korea is recognized as an important cause of many atopic asthma. Recent studies stress the significance of Alternaria as a cause of respiratory problems although detailed knowledge on this organism is limited, especially the relationship of the fungi to respiratory ailments as well as the allergenic nature of this organism. METHODS: The authors studied the purification methods of Alternaria allergen and examine how the allergen acts on atopic asthma. The Alternaria samples were cultured in potato-dextrose broth for 10 days, and a crude extract was obtained after dialysis and filtration. The crude extract was subjected to SDS-PAGE followed by staining, and the allergen was analyzed. Three subjects who showed positive allergy skin test to Alternaria were subjected to RAST and allergy skin test with the crude extract. RESULTS: SDS-PAGE of the curde extract showed more than 30 allergenic bands, and among those, 5 kD, 28 kD, 31 kD and 34 kD bands were the major allergens, indicating that the purification method used in the present study produced an extract with almost all major allergens. In 3 patients with Alternaria allergy we carried out allergy skin test with crude extracts. All three subjects showed positive allergy skin test. CONCLUSION: The crude extract obtained major allergens including 70 kD, 65 kD, 34 kD, 31 kD, 28 kD and 16 kD allergens, and like the results reported outside Korea. Alternaria was proved to be a major allergen in atopic asthma in Korea as well.
Allergens* ; Alternaria* ; Asthma ; Complex Mixtures ; Dialysis ; Electrophoresis, Polyacrylamide Gel ; Filtration ; Fungi ; Humans ; Hypersensitivity ; Korea ; Skin Tests

PURPOSE: Right middle lobe symdrome is characterized by a spectrum of disease from recurrent atelectasis and pneunomitis to brobchiectasis of the Right middle lobe symdrom. It was first reported gy Grahm describing 12 patients with middle loge atelectasis and bronchiectasis due to enlarged lymph nodes. The incidence of Right middle lobe syndome seems tobe increasing in children, byt there have been only a few studies of right middlelobe syndrome in Korea. METHODS: Twenty-five children with RMLS who had been admitted during the last 10 years were evaluated with particular attention to clinical features, laboratory results, bronchographic findings, and treatment RESULTS: All patients were symptomatic and complained of chronic cough(25), sputum(20), fever(16), dyspnea(3), vomiting(2), and foreign body in the bronchus(2). Most of the patients had recurrent pneumonia: 6 patients had Mycoplasma pneumonia, and 6 patients had ashma and allergic disorders. Only 5 out of the 25 patients showed sufficient obstruction on bronchography and 6 patients took computed tomography scans. Chest radiography, bronchography and computed tomography scans were evaluated for review in 25 patients showing consolidation(17), patchy infiltration(14), atelectasis(12), hyperinflation(5), bronchiectasis(2), and air bronchogram(2). Most patients were improved by conservative medical management and only 2 patients had closed thoracostomy. CONCLUSION: These 25 patients who had been diagnosed as Right middle lobe syndrome were improved after 2 week treatment of antibiotics and conservative management and their prognosis were good during the follow-up period.
Anti-Bacterial Agents ; Bronchiectasis ; Bronchography ; Child ; Follow-Up Studies ; Foreign Bodies ; Humans ; Incidence ; Korea ; Lymph Nodes ; Middle Lobe Syndrome* ; Pneumonia ; Pneumonia, Mycoplasma ; Prognosis ; Pulmonary Atelectasis ; Radiography ; Thoracostomy ; Thorax

PURPOSE: A Cow's milk allergy (CMA) is one of common problems during first year of life. Though milk caused allergic problems, their parents didn't know it, and gave milk to their children. But the parents don't give milk to their children if they know it. Therefore it may cause problems of growth and development in children. The purpose of this study is to analyze those clinical features of milk allergy confirmed by oral provocation test. METHODS: We carried out the retrospective study on 21 patients who had been performed oral milk provocation test at Yonsei university children's allergic clinic. RESULTS: 9 out of 21cases (43%) showed positive milk oral provocation test. The most common clinical symptom & sign was urticaria, followed by respiratory symptoms and abdominal symptoms. The rate which past history and positive skin test corresponded to oral provocation test was very high (100%). CONCLUSION: The most valuable diagnosis of food allergy is oral provocation test. However, predictive value of allergy skin test and past history was very high in milk allergy.
Child ; Diagnosis ; Food Hypersensitivity ; Growth and Development ; Humans ; Hypersensitivity ; Milk Hypersensitivity ; Milk* ; Parents ; Retrospective Studies ; Skin Tests ; Urticaria