In canine acute pancreatitis (AP), inappropriate release and activation of zymogen proteases within the pancreas results in the consumption of serum antiproteases. The aim of this study was to examine whether the serum concentrations of α₂-macroglobulin (A2MG), α₁-antitrypsin (A1AT), and C-reactive protein (CRP) differ between dogs with AP and healthy dogs. Twenty healthy dogs and 20 dogs with AP were included in this study. Concentrations of A2MG, A1AT, and CRP were measured in the sera of healthy dogs and dogs diagnosed with AP. Serum A2MG and A1AT concentrations were significantly lower in dogs with AP than in healthy dogs, whereas the serum CRP concentration was significantly higher. In addition, the concentrations of A2MG and A1AT were significantly higher in AP survivors than in AP non-survivors, while the CRP concentration was significantly lower. However, in both AP survivors and non-survivors, the CRP concentrations showed a negative correlation with A2MG concentrations but not with A1AT. These findings indicate that serum antiproteases and CRP concentrations might be associated with the mortality rate of AP in dogs.
Animals ; C-Reactive Protein ; Dogs ; Humans ; Mortality ; Pancreas ; Pancreatitis ; Peptide Hydrolases ; Protease Inhibitors ; Survivors ; Trypsin

Abstract: Portable blood glucose meters (PBGMs) are widely used because of their practicality. However, the accuracy of PBGMs has frequently been questioned. The objectives of this study were to evaluate factors that might interfere with measurements made using PBGMs, and to assess the clinical utility of 6 PBGMs. The glucose concentrations measured using the PBGMs were compared with those obtained using a reference method. The agreement between the measured values was assessed using Spearman correlation analysis, Passing-Bablok regression analysis, Bland-Altman plots, and consensus error grid analysis. Mann-Whitney and KruskalWallis tests were performed to identify the parameters affecting glucose measurement. The results indicated that all of the PBGMs tested perform adequately for use in veterinary practice. In most cases, measurements made using PBGM corresponded well with the blood glucose values obtained using the reference method. Error grid analysis revealed that most of the PBGM values fell within zones A and B. However, some measurements of blood glucose concentrations < 80 mg/dL fell into zone C. PCV, and triglyceride and total protein concentration, significantly affected the output of some of the PBGMs. Therefore, clinicians should be aware of the characteristics of the PBGM that they use.

In canine acute pancreatitis (AP), inappropriate release and activation of zymogen proteases within the pancreas results in the consumption of serum antiproteases. The aim of this study was to examine whether the serum concentrations of α₂-macroglobulin (A2MG), α₁-antitrypsin (A1AT), and C-reactive protein (CRP) differ between dogs with AP and healthy dogs. Twenty healthy dogs and 20 dogs with AP were included in this study. Concentrations of A2MG, A1AT, and CRP were measured in the sera of healthy dogs and dogs diagnosed with AP. Serum A2MG and A1AT concentrations were significantly lower in dogs with AP than in healthy dogs, whereas the serum CRP concentration was significantly higher. In addition, the concentrations of A2MG and A1AT were significantly higher in AP survivors than in AP non-survivors, while the CRP concentration was significantly lower. However, in both AP survivors and non-survivors, the CRP concentrations showed a negative correlation with A2MG concentrations but not with A1AT. These findings indicate that serum antiproteases and CRP concentrations might be associated with the mortality rate of AP in dogs.

Three small breed dogs were referred for the evaluation of neurologic deficits. Upon physical and neurologic examination, all dogs displayed hyperesthesia, pain, and neck stiffness. Magnetic resonance imaging was performed on the brain and spinal cord, and all three dogs presented Chiari-like malformations and syringomyelia. These dogs were treated with prednisolone and furosemide, and showed rapid improvement of clinical signs. Chiari malformations and syringomyelia were not improved because of congenital disorders. This case report demonstrates the clinical and diagnostic features of Chiari-like malformations and syringomyelia in three small breed dogs.
Animals ; Anti-Inflammatory Agents/therapeutic use ; Arnold-Chiari Malformation/pathology/*veterinary ; Body Size ; Diuretics/therapeutic use ; Dog Diseases/drug therapy/*pathology ; Dogs ; Female ; Furosemide/therapeutic use ; Male ; Prednisolone/therapeutic use ; Syringomyelia/drug therapy/pathology/*veterinary

The prevalence of feline herpesvirus-1 (FHV-1), feline calicivirus (FCV), and Chlamydophila (C.) felis was studied in cats of an animal shelter in Korea. Total 78 cats without ocular and upper respiratory tract disease were examined. Specimens were obtained from ocular conjunctiva and oropharynx. Using multiplex polymerase chain reaction (PCR) and reverse transcription PCR, three pathogens were simultaneously detected. In examined 78 cats, 49 (63%) cats were positive for FHV-1. However, all specimens were negative for C. felis and FCV. In conclusion, many cats recovered from FHV-1 infection remain subclinical carriers in shelter environment.
Animals ; Caliciviridae/genetics ; Caliciviridae Infections/epidemiology/*veterinary ; Cat Diseases/*epidemiology/*microbiology/*virology ; Cats ; Chlamydophila/genetics ; Chlamydophila Infections/epidemiology/*veterinary ; DNA Primers/genetics ; Herpesviridae/genetics ; Herpesviridae Infections/epidemiology/*veterinary ; Housing, Animal ; Korea/epidemiology ; Prevalence ; Reverse Transcriptase Polymerase Chain Reaction

Allergic disorders are exaggerated immune responses to foreign antigens, regardless of the mechanism, while atopic disorders are exaggerated IgE-mediated immune responses (type I hypersensitivity). Allergic dermatitis is a common pathological condition of skin in humans and dogs. Canine allergic dermatitis presents with clinical signs similar to those reported in humans, and its causes are complex; therefore, diagnostic tests and treatments may need to be adjusted for each patient. Dogs with allergic dermatitis can suffer from secondary infections, which must be considered and confirmed or excluded for successful treatment. In this report, 35 cases of canine allergic dermatitis diagnosed using variable methods, including histological and cytological examination, are described. Patients were treated with oral or topical medications (antimicrobials, anti-inflammatories, immune modulators, topical ointments, and medicated shampoos), and their diets and environmental surroundings were also modified. This report provides an analysis of the breed, gender, age of onset, clinical signs, diagnostic methods, and treatments for canine allergic dermatitis. The information on canine allergic dermatitis presented here could be helpful in the study of human cases because these two species often share living spaces, environments, and lifestyles more closely than other animals. However, previous reports have suggested that human and canine allergies differ in some features, such as involvement of histamine in induction of pruritus, and in histopathological characteristics such as cutaneous structures.
Age of Onset ; Animals ; Anti-Inflammatory Agents ; Coinfection ; Dermatitis* ; Diagnostic Tests, Routine ; Diet ; Dogs* ; Histamine ; Humans ; Hypersensitivity ; Life Style ; Methods ; Ointments ; Pruritus ; Retrospective Studies* ; Skin

This study assessed the effects of probiotics in canine atopic dermatitis (AD). We enrolled 11 client-owned dogs with AD and randomly allocated them to two groups. The probiotics group (n = 7) was prescribed with Bifidobacterium longum while the control group (n = 4) received a placebo powder once a day for 12 weeks. In both groups, the degree of skin lesions was evaluated based on the Canine Atopic Dermatitis Extent and Severity Index (CADESI)-4. We also measured the transepidermal water loss (TEWL). We assessed pruritus severity using the pruritus visual analog scale (PVAS). Alteration of consumed drug doses was converted into medication scores. All the evaluation indices were surveyed every 4 weeks. In the probiotics group, there was a significant decrease in the CADESI-4 score at 4, 8, and 12 weeks compared to that of the baseline score (p < 0.05). There was no significant difference in TEWL, PVAS, and medication score at each time point and between groups. Although these results showed that Bifidobacterium longum did not reduce pruritus, TEWL, and the dosage of drugs for canine AD, it was effective in improving skin lesions, therefore, probiotics could be considered in canine AD with severe skin symptoms.

Pruritus is one of the most important symptoms of allergic inflammatory skin disease. Conjugated linoleic acid (CLA) has been reported to have preventive effects against allergic inflammation. The objective of this study was to determine whether or not oral administration of CLA suppresses pruritus induced by compound 48/80 (composed of N-methyl-p-methoxy phenethylamine with formaldehyde) in mice, and if so, whether or not this effect is associated with serum histamine and prostaglandin (PG) E2 levels. Liquid CLA mixture (36.25% 9c-11t CLA, 36.95% 10t-12c CLA, 1.12% 9c-11c, and 1.94% t9-t11 CLA) was emulsified in 0.5% carboxymethyl cellulose (CMC) sodium salt and orally administered to mice at doses of 200 mg/kg once per day for 3 days. Similarly, disodium chromoglycate (DSCG), an antipruritic substance, was administered orally at the same concentrations as the negative control. Compound 48/80, a pruritus-inducing reagent, was subcutaneously injected 30 minutes after final administration of CLA. Scratching behavior of mice was counted just after compound 48/80 injection. Serum histamine and PGE2 concentrations were evaluated individually. Mice administered with CLA showed reduced frequency of scratching behavior compared to those without CLA. Antipruritic activities in CLA-treated and DSCG-treated groups were 48.5% and 26.8%, respectively. CLA and DSCG also diminished serum concentrations of histamine and PGE2 compared to compound 48/80 alone, respectively. This result suggests that dietary CLA has an antipruritic effect by down-regulating serum histamine and PGE2 levels for relief of compound 48/80-induced scratching behavior in mice, which will be useful in allergic pruritus as a preventive medicine.
Administration, Oral ; Animals ; Carboxymethylcellulose Sodium ; Dinoprostone ; Histamine ; Inflammation ; Linoleic Acid* ; Mice* ; Preventive Medicine ; Pruritus* ; Skin Diseases ; Sodium

A-7-month-old, intact male Shih-Tzu dog was referred with facial dermatitis and stifle pain with 7 days duration. Erythema, hemorrhage, and crusted nodular lesions of the face, swollen eyelids, and otitis externa were observed. A painful response was noted on palpation of the right stifle joint. Impression smears and skin biopsies revealed pyogranulomatous inflammation consistent with canine juvenile cellulitis (CJC). Skin lesions and pain were greatly improved following immunosuppressive corticosteroid therapy. This report firstly describes clinical and histopathological findings as well as treatment responses of CJC in a 7-month-old, domestic dog.
Animals ; Biopsy ; Cellulitis* ; Dermatitis ; Dogs* ; Erythema ; Eyelids ; Hemorrhage ; Humans ; Infant* ; Inflammation ; Joints ; Male ; Otitis Externa ; Palpation ; Skin ; Stifle

A 5-year-old, 2.7 kg, spayed female Scottish Fold cat presented with hematemesis after administration of oral zaltoprofen, a non-steroidal anti-inflammatory drug, by the owner. Diagnostic imaging and blood analyses indicated development of acute kidney injury (AKI) resulting from zaltoprofen ingestion. To correct dehydration and anemic conditions, the cat received intravenous fluid therapy with whole blood transfusion and peroral N-acetylcysteine. Clinical signs resolved, but persistent azotemia was unresolved indicating that AKI could progress to chronic kidney disease. This case suggests that although zaltoprofen may have low adverse effects on humans, administration of zaltoprofen in cats can have serious adverse effects.
Acetylcysteine ; Acute Kidney Injury* ; Animals ; Anti-Inflammatory Agents, Non-Steroidal ; Azotemia ; Blood Transfusion ; Cats* ; Child, Preschool ; Dehydration ; Diagnostic Imaging ; Eating ; Female ; Fluid Therapy ; Hematemesis ; Humans ; Renal Insufficiency ; Renal Insufficiency, Chronic