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Due to their similarities with humans in anatomy, physiology, and genetics miniature pigs are becoming an attractive model for biomedical research. We aim to establish and evaluate blood type O cells derived from Korean native pig (KNP), a typical miniature pig breed in Korea. Ten cell lines derived from 8 KNP piglets and one adult female KNP (kidney and ear tissues) were established. To confirm the presence of blood type O, genomic DNA, fucosyltransferase (FUT) expression, and immunofluorescence staining were examined. Additionally, fluorescence-activated cell sorting and somatic cell nuclear transfer were performed to investigate the normality of the cell lines and to evaluate their effectiveness in embryo development. We found no significant bands corresponding to specific blood group A, and no increase in FUT expression in cell lines derived from piglets No. 1, No. 4, No. 5, No. 8, and the adult female KNP; moreover, they showed normal levels of expression of α 1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase. There was no significant difference in embryo development between skin and kidney fibroblasts derived from the blood type O KNPs. In conclusion, we successfully established blood type O KNP cell lines, which may serve as a useful model in xenotransplantation research.
Adult ; Cell Line ; Cytidine ; DNA ; Ear ; Embryonic Development ; Female ; Fibroblasts ; Flow Cytometry ; Fluorescent Antibody Technique ; Genetics ; Heterografts ; Humans ; Kidney ; Korea ; Physiology ; Pregnancy ; Skin ; Swine ; Swine, Miniature ; Transplantation, Heterologous

10.Convulsion, Symptomatic Hyponatremia and Paralytic Ileus Following Tonsillectomy: A case report.

Il Young CHEONG ; Eui Cheol NAM ; Jun Yeon WON ; Sung Yeon AHN ; Ja Kyoung KIM ; Seong Sik KANG ; Hee Jeong SON ; Byeong Moon HWANG

Korean Journal of Anesthesiology 2005;49(1):101-105

Severe symptomatic hyponatremia shows high mortality in association with cerebral edema and central nervous system dysfunction. Postoperative hyponatremia is usually attributed to administration of hypotonic fluids while antidiuretic hormone is acting. However, we experienced a severe symptomatic hyponatremia in spite of infusion of lactated Ringer's solution perioperatively in a case of 4-year-old girl's tonsillectomy. Inappropriate secretion of ADH caused by pain, stress, anxiety, nausea, vomiting. Paralytic ileus developed several hours after surgery, severe hyponatremia (Na 119 mmol/L) with convulsion notified. After prompt infusion of sodium supplement and fluid restriction, the patient recovered uneventfully.
Anxiety ; Brain Edema ; Central Nervous System ; Child, Preschool ; Humans ; Hyponatremia* ; Intestinal Pseudo-Obstruction* ; Mortality ; Nausea ; Seizures* ; Sodium ; Tonsillectomy* ; Vomiting

1.Combined intrauterine and intraligamentary full term pregnancy after in vitro fertilization & embryo transfer.

2.A case of myasthenia gravis in pregnancy.

3.The study on successful therapy with electroejaculation and in vitro fertilization-embryo trnasfer.

4.Dermographic change of aging female population in Korea.

5.Dermographic change of aging female population in Korea.

6.Doppler blood flow velocity waveforms of the fetal descending thoracic aorta trough the nomal pregnancy.

7.A case of presacral teratoma.

8.A study on psychological strain IVF.

9.Establishment and identification of cell lines from type O blood Korean native pigs and their efficiency in supporting embryonic development via somatic cell nuclear transfer

10.Convulsion, Symptomatic Hyponatremia and Paralytic Ileus Following Tonsillectomy: A case report.

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