1.Cryopreservation of Testicular Spermatozoa using Mouse Zona Pellucida in Intracytoplasmic Sperm Injection Program.
Tae Kwang SUH ; Byeong Gyun JEON ; Eun Kyung RYU ; Eun Sook LEE ; Zae Yoong RYOO ; Sea Hwan SOHN ; Jin Soo MOON ; Kwang Chull KIM
Korean Journal of Fertility and Sterility 1997;24(2):187-192
The survival rate and motility recovered after cryopreservation of testicular spermatozoa in testicular sperm extraction (TESE)-ICSl program is low. The purpose of this study was to assess the availability and efficiency of mouse empty zona pellucida in cryopreserving human TESE spermatozoa. Mouse empty zonae pellucidae were obtained by extraction of cytoplasm with or without cytochalasin B treatment. Motile sperm from proven-fertile donor and two azoospermic patients after TESE were individually inserted into empty zona pellucida and cryopreserved. Two to five days after cyropreservation, the frozen sperm were thawed and the rates of recovery and motility were observed. The ooplasmic extraction rates of control (N=80) and cytochalasin B treated oocytes (N=80) were 94.0% and 96.2%, respectively (p>0.05). The post-thaw recovery rates of spermatozoa and rates of motility recovery of ejaculate (N=70) and testicular (N=70) sperm were 97.1%, 97.1% and 95.7%, 94.3%, respectively (p>0.05). The results of this study showed that the mouse zone pellucida is useful for cryostorage of single testicular spermatozoa.
Animals
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Cryopreservation*
;
Cytochalasin B
;
Cytoplasm
;
Herpes Zoster*
;
Humans
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Mice*
;
Oocytes
;
Sperm Injections, Intracytoplasmic*
;
Spermatozoa*
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Survival Rate
;
Tissue Donors
;
Zona Pellucida*
2.Diagnostic value of prostate health index in patients with no index lesion on mpMRI or negative previous combined biopsy
Seong Soo JEON ; Wan SONG ; Minyong KANG ; Hyun Hwan SUNG ; Hwang Gyun JEON ; Byeong Chang JEONG ; Seong Il SEO ; Jae Hoon CHUNG
Investigative and Clinical Urology 2025;66(2):124-129
Purpose:
To assess the effectiveness of the prostate health index (PHI) in patients with no index lesions on multiparametric magnetic resonance imaging (mpMRI) or with negative findings on past prostate biopsy if there was an index lesion on mpMRI.
Materials and Methods:
Patients without an index lesion on MRI or with a negative result on combined biopsy for index lesions were assessed. Patients who underwent transperineal mapping biopsy among those suspected of having prostate cancer (PCa) due to persistently elevated prostate-specific antigen (PSA) levels were analyzed.
Results:
Of the 291 patients, 82 (28.2%) were diagnosed with PCa. Sixty-five of 291 patients had negative finding in previous combined biopsy. In total, 226 patients did not have any index lesions. The mean age of the PCa group was 64.33±8.88 years and that of the non-cancer group was 59.88±10.26 years (p<0.001). The PHI was 46.75±28.22 in the PCa group and 37.74±17.37 in the noncancer group (p=0.001), and the prostate volume was 41.52±15.77 mL in the PCa group and 50.78±23.97 mL in the non-cancer group (p=0.001). In multivariate analysis, age (odds ratio [OR] 1.096, p<0.001), PHI (OR 1.021, p=0.005), and prostate volume (OR 0.954, p<0.001) were identified as significant factors for PCa detection. The optimal cutoff value of the PHI for PCa detection was 44.6 and the PHI density (PHID) was 0.88.
Conclusions
In patients with elevated PSA levels but no index lesions on mpMRI or negative biopsy findings, PHI and PHID demonstrated significant potential for improving PCa detection.
3.Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI).
Taek Hoo LEE ; Hang Jin KIM ; Gun Ho SONG ; Dae Geun KIM ; Sang Sik CHUN ; Yoon Kyu PARK ; Tae Kwang SUH ; Byeong Gyun JEON ; Eun Kyung RYU ; Eun Sook LEE ; Jin Soo MOON ; Kwang Chull KIM
Korean Journal of Obstetrics and Gynecology 1997;40(12):2741-2746
No abstract available.
Blastocyst
;
Coculture Techniques
;
Fertilization
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Oocytes*
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Sperm Injections, Intracytoplasmic
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Sperm Retrieval*
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Spermatozoa*