1.Electro-acupuncture promotes gut motility and alleviates functional constipation by regulating gut microbiota and increasing butyric acid generation in mice.
Ming-Min XU ; Yu GUO ; Ying CHEN ; Wei ZHANG ; Lu WANG ; Ying LI
Journal of Integrative Medicine 2023;21(4):397-406
OBJECTIVE:
Abnormalities in the gut microbiota and intestinal short-chain fatty acid (SCFA) levels are implicated in the pathogenesis of functional constipation (FC). Electro-acupuncture (EA) has been shown to improve constipation-related symptoms and rebalance the gut microbiota. However, it is currently unknown whether the gut microbiota is a key mechanistic target for EA or how EA promotes gut motility by regulating the gut microbiota and SCFAs. Therefore, we assessed the effects of EA in FC mice and pseudo-germfree (PGF) mice to address these questions.
METHODS:
Forty female Kunming mice were randomly separated into a normal control group (n = 8), an FC group (n = 8), an FC + EA group (n = 8), a PGF group (n = 8) and a PGF + EA group (n = 8). The FC group and FC + EA group were treated with diphenoxylate to establish the FC model; the PGF group and PGF + EA group were given an antibiotic cocktail to initiate the PGF model. After maintaining the model for 14 d, mice in the FC + EA and PGF + EA groups received EA stimulation at the ST25 and ST37 acupoints, once a day, 5 times per week, for 2 weeks. Fecal parameters and intestinal transit rate were calculated to assess the efficacy of EA on constipation and gastrointestinal motility. Colonic contents were used to quantify gut microbial diversity using 16S rRNA sequencing, and measure SCFA concentrations using gas chromatography-mass spectrometry.
RESULTS:
EA significantly shortened the first black stool defecation time (P < 0.05) and increased the intestinal transit rate (P < 0.01), and fecal pellet number (P < 0.05), wet weight (P < 0.05) and water content (P < 0.01) over 8 h, compared with the FC group, showing that EA promoted gut motility and alleviated constipation. However, EA treatment did not reverse slow-transit colonic motility in PGF mice (P > 0.05), demonstrating that the gut microbiota may play a mechanistic role in the EA treatment of constipation. In addition, EA treatment restored the Firmicutes to Bacteroidetes ratio and significantly increased butyric acid generation in FC mice (P < 0.05), most likely due to the upregulation of Staphylococcaceae microorganisms (P < 0.01).
CONCLUSION
EA-mediated resolution of constipation occurs through rebalancing the gut microbiota and promoting butyric acid generation. Please cite this article as: Xu MM, Guo Y, Chen Y, Zhang W, Wang L, Li Y. Electro-acupuncture promotes gut motility and alleviates functional constipation by regulating gut microbiota and increasing butyric acid generation in mice. J Integr Med. 2023; Epub ahead of print.
Mice
;
Female
;
Animals
;
Gastrointestinal Microbiome
;
Butyric Acid/pharmacology*
;
RNA, Ribosomal, 16S/genetics*
;
Constipation/therapy*
;
Acupuncture Therapy
;
Electroacupuncture/methods*
2.Effects of sodium butyrate on proliferation of human oral squamous carcinoma cell line and expression of p27Kip1.
Ling GONG ; Wen-mei WANG ; Yan JI ; Yu WANG ; Da-wei LI
Chinese Journal of Stomatology 2010;45(10):619-622
OBJECTIVETo investigate the effect of sodium butyrate (NaB) on inhibition of human oral squamous carcinoma cell line.
METHODSHuman oral squamous carcinoma cell line Tca8113 was treated with different concentration of NaB. Light microscope was used to observe the morphological changes of the carcinoma cells. The cell cycle was analyzed by flow cytometry. Expression of p27(Kip1) was determined with immunocytochemical assay.
RESULTSNaB significantly inhibited the proliferation of Tca8113 in a time- and dose-dependent manner. Tca8113 cells treated with NaB was arrested in G(0)-G(1) phase. The fraction of cells in G(0)-G(1) were (63.2 ± 2.4)% and (77.2 ± 3.8)% after treated with NaB at the concentration of 2 and 4 mmol/L alternatively, whereas (48.1 ± 2.4)% in G(0)-G(1) phase were observed in control group (P < 0.05). The expression of p27(Kip1) was markedly up-regulated after being treated with NaB.
CONCLUSIONSNaB treatment can inhibit the growth of oral squamous carcinoma cell line in vitro and induce cell cycle arrest, which might be associated with the increased expression of p27(Kip1) protein.
Apoptosis ; Butyric Acid ; pharmacology ; Carcinoma, Squamous Cell ; pathology ; Cell Cycle ; Cell Division ; Cell Line, Tumor ; Cyclin-Dependent Kinase Inhibitor p27 ; biosynthesis ; Humans ; Mouth Neoplasms ; pathology ; Up-Regulation
3.Transient folate deprivation in combination with small-molecule compounds facilitates the generation of somatic cell-derived pluripotent stem cells in mice.
Wen-tao HU ; Qiu-yue YAN ; Yu FANG ; Zhan-dong QIU ; Su-ming ZHANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(2):151-156
Induced pluripotent stem cells (iPSCs) can be propagated indefinitely, while maintaining the capacity to differentiate into all cell types in the body except for the extra-embryonic tissues. This iPSC technology not only represents a new way to use individual-specific stem cells for regenerative medicine but also constitutes a novel method to obtain large numbers of disease-specific cells for biomedical research. However, the low efficiency of reprogramming and genomic integration of oncogenes and viral vectors limit the potential application of iPSCs. Chemical-induced reprogramming offers a novel approach to generating iPSCs. In this study, a new combination of small-molecule compounds (SMs) (sodium butyrate, A-83-01, CHIR99021, Y-27632) under conditions of transient folate deprivation was used to generate iPSC. It was found that transient folate deprivation combined with SMs was sufficient to permit reprogramming from mouse embryonic fibroblasts (MEFs) in the presence of transcription factors, Oct4 and Klf4, within 25 days, replacing Sox2 and c-Myc, and accelerated the generation of mouse iPSCs. The resulting cell lines resembled mouse embryonic stem (ES) cells with respect to proliferation rate, morphology, pluripotency-associated markers and gene expressions. Deprivation of folic acid, combined with treating MEFs with SMs, can improve the inducing efficiency of iPSCs and reduce their carcinogenicity and the use of exogenous reprogramming factors.
Amides
;
pharmacology
;
Animals
;
Butyric Acid
;
pharmacology
;
Cell Differentiation
;
drug effects
;
Cell Line
;
Cell Proliferation
;
drug effects
;
Extraembryonic Membranes
;
cytology
;
drug effects
;
Folic Acid
;
pharmacology
;
Induced Pluripotent Stem Cells
;
cytology
;
drug effects
;
Kruppel-Like Transcription Factors
;
metabolism
;
Mice
;
Octamer Transcription Factor-3
;
metabolism
;
Proto-Oncogene Proteins c-myc
;
metabolism
;
Pyrazoles
;
pharmacology
;
Pyridines
;
pharmacology
;
Pyrimidines
;
pharmacology
;
SOXB1 Transcription Factors
;
metabolism
;
Thiocarbamates
;
pharmacology
;
Thiosemicarbazones
4.Protective effects of sodium butyrate against lung injury in mice with endotoxemia.
Acta Physiologica Sinica 2012;64(3):308-312
The aim of the present study was to investigate the effects of sodium butyrate (SB) on systemic inflammation, lung injury and survival rate of mice with endotoxemia. Balb/c mice were pre-treated with SB or vehicle, and then endotoxemia was induced by lethal dose of lipopolysaccharide (LPS, 20 mg/kg, i.p.) and the survival rate of mice was monitored. A separated set of animals were sacrificed at 18 h after LPS challenge, and blood samples were harvested for measuring TNF-α and IL-6 levels. Lung tissues were also harvested to determine the ratio of wet weight to dry weight of lung tissue and myeloperoxidase (MPO) activity in lung tissue. In addition, the formalin-fixed lung specimens were stained with HE routinely for morphologic evaluation. The results showed that pre-treatment with SB alleviated LPS-induced morphological damage in lung tissue. This was accompanied by reduced ratio of wet weight to dry weight of lung tissue and MPO activity in lung homogenates. Additionally, the up-regulation of pro-inflammatory cytokines TNF-α and IL-6 was also suppressed by SB, while the survival rate of mice with lethal endotoxemia was significantly increased by SB pre-treatment. The results suggest that SB effectively attenuates intrapulmonary inflammatory response and improves the survival of endotoxemic mice.
Animals
;
Butyric Acid
;
pharmacology
;
Endotoxemia
;
drug therapy
;
Inflammation
;
drug therapy
;
Interleukin-6
;
metabolism
;
Lipopolysaccharides
;
Lung
;
drug effects
;
pathology
;
Lung Injury
;
drug therapy
;
Male
;
Mice
;
Mice, Inbred BALB C
;
Peroxidase
;
metabolism
;
Tumor Necrosis Factor-alpha
;
metabolism
5.Sodium butyrate inhibits HMGB1 expression and release and attenuates concanavalin A-induced acute liver injury in mice.
Quan GONG ; Mao-Jian CHEN ; Chao WANG ; Hao NIE ; Yan-Xiang ZHANG ; Ke-Gang SHU ; Gang LI
Acta Physiologica Sinica 2014;66(5):619-624
The purpose of the present study is to explore the protective effects of sodium butyrate (SB) pretreatment on concanavalin A (Con A)-induced acute liver injury in mice. The model animals were first administered intraperitoneally with SB. Half an hour later, acute liver injury mouse model was established by caudal vein injection with Con A (15 mg/kg). Then, levels of serous alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using standard clinical method by an automated chemistry analyzer, tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) were measured by ELISA, and pathological changes in hepatic tissue were observed by using HE staining and light microscopy. The expression and release of high-mobility group box 1 (HMGB1) were assessed by using reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry and ELISA. The results showed that the pretreatment of SB significantly protected Con A-treated mice from liver injury as evidenced by the decrease of serum ALT, AST (P < 0.01) and reduction of hepatic tissues necrosis. SB also decreased levels of serous TNF-α and IFN-γ (P < 0.01). Furthermore, the expression and release of HMGB1 were markedly inhibited by SB pretreatment (P < 0.05 or P < 0.01). These results suggest that the attenuating effect of SB on Con A-induced acute liver injury may be due to its role of reducing the TNF-α and IFN-γ production, and inhibiting HMGB1 expression and release.
Alanine Transaminase
;
metabolism
;
Animals
;
Aspartate Aminotransferases
;
metabolism
;
Butyric Acid
;
pharmacology
;
Chemical and Drug Induced Liver Injury
;
drug therapy
;
Concanavalin A
;
adverse effects
;
Disease Models, Animal
;
HMGB1 Protein
;
metabolism
;
Interferon-gamma
;
metabolism
;
Liver
;
pathology
;
Mice
;
Tumor Necrosis Factor-alpha
;
metabolism
6.Sodium butyrate induces apoptosis of human colon cancer cells by modulating ERK and sphingosine kinase 2.
Min XIAO ; Yun Gang LIU ; Meng Chen ZOU ; Fei ZOU
Biomedical and Environmental Sciences 2014;27(3):197-203
OBJECTIVETo investigate the role of extracellular signal-regulated kinase (ERK) in apoptosis of human colon cancer (HCT116) cells.
METHODSAfter the HCT116 cells were pretreated with specific ERK inhibitor (U0126) or specific siRNA and exposed to 10 mmol/L sodium butyrate (NaBT) for 24 h, their apoptosis was detected by flow cytometry, levels of SphK2 and ERK protein were measured by Western blot, and translocation of SphK2 was assayed by immunofluorescence microscopy.
RESULTSThe U0126 and siRNAs specific for SphK2 blocked the export of SphK2 from nuclei to cytoplasm and increased the apoptosis of HCT116 cells following NaBT exposure. Over-expression of PKD decreased NaBT-induced apoptosis of HCT116 cells, which was reversed by U0126. Furthermore, transfection of HCT116 cells with constitutively activated PKD plasmids recovered the U0126-blocked export of SphK2.
CONCLUSIONERK regulates the export of SphK2 and apoptosis of HCT116 cells by modulating PKD. Modulation of these molecules may help increase the sensitivity of colon cancer cells to the physiologic anti-colon cancer agent, NaBT.
Apoptosis ; drug effects ; physiology ; Butyric Acid ; pharmacology ; Extracellular Signal-Regulated MAP Kinases ; metabolism ; HCT116 Cells ; drug effects ; Humans ; Phosphotransferases (Alcohol Group Acceptor) ; genetics ; metabolism ; Protein Kinase C ; genetics ; metabolism ; RNA, Small Interfering ; Signal Transduction ; drug effects
7.Butyrate regulates leptin expression through different signaling pathways in adipocytes.
Mohamed Mohamed SOLIMAN ; Mohamed Mohamed AHMED ; Alaa Eldin SALAH-ELDIN ; Abeer Abdel Alim ABDEL-AAL
Journal of Veterinary Science 2011;12(4):319-323
Leptin is an adipocytokine that regulates body weight, and maintains energy homeostasis by promoting reduced food intake and increasing energy expenditure. Leptin expression and secretion is regulated by various factors including hormones and fatty acids. Butyrate is a short-chain fatty acid that acts as source of energy in humans. We determined whether this fatty acid can play a role in leptin expression in fully differentiated human adipocytes. Mature differentiated adipocytes were incubated with or without increasing concentrations of butyrate. RNA was extracted and leptin mRNA expression was examined by Northern blot analysis. Moreover, the cells were incubated with regulators that may affect signals which may alter leptin expression and analyzed with Northern blotting. Butyrate stimulated leptin expression, and stimulated mitogen activated protein kinase (MAPK) and phospho-CREB signaling in a time-dependent manner. Prior treatment of the cells with signal transduction inhibitors as pertusis toxin, Gi protein antagonist, PD98059 (a MAPK inhibitor), and wortmannin (a PI3K inhibitor) abolished leptin mRNA expression. These results suggest that butyrate can regulate leptin expression in humans at the transcriptional level. This is accomplished by: 1) Gi protein-coupled receptors specific for short-chain fatty acids, and 2) MAPK and phosphatidylinositol-3-kinase (PI3K) signaling pathways.
Adipocytes/*metabolism
;
Azo Compounds
;
Butyric Acid/*pharmacology
;
CREB-Binding Protein/genetics/metabolism
;
Cell Differentiation
;
Cells, Cultured
;
Gene Expression Regulation/*drug effects/physiology
;
Humans
;
Leptin/genetics/*metabolism
;
Mitogen-Activated Protein Kinase Kinases/genetics/metabolism
;
Phosphatidylinositol 3-Kinases/genetics/metabolism
;
RNA, Messenger/genetics/metabolism
;
Signal Transduction/*physiology
;
Staining and Labeling