2.Research and industrialization of biobased materials in China.
Chinese Journal of Biotechnology 2015;31(6):955-967
This paper reviews the research and commercialization progresses of biobased polymeric materials including polyhydroxyalkanoates (PHA), polylactides (PLA), poly (butylene succinate) (PBS) and its monomer succinate, and CO2 copolymer poly (propylene carbonate), especially these efforts made in China.
Bioengineering
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Butylene Glycols
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China
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Polyesters
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Polyhydroxyalkanoates
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Polymers
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Propane
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analogs & derivatives
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Succinic Acid
3.Chemical constituents from roots of Machilus yaoshansis.
Bo LIU ; Mingtao LIU ; Maoluo GAN ; Feng ZHAO ; Xiuli WU ; Yang YU ; Zhenggang YUE ; Sheng LIN ; Sujuan WANG ; Chenggen ZHU ; Jiangong SHI
China Journal of Chinese Materia Medica 2012;37(9):1227-1231
To study chemical constituents contained in ethanol extracts from roots of Machilus yaoshansis. Fifteen compounds were separated from the roots of M. yaoshansis by using various chromatographic techniques. Their structures were identified on the basis of their physicochemical properties and spectral data as twelve lignans(+)-guaiacin (1), kadsuralignan C (2), (+)-isolariciresinol (3), 5'-methoxy-(+)-isolariciresinol (4), (7'S, 8R, 8'R)-lyoniresinol (5), meso-secoisolariciresinol (6), isolariciresinol-9'-O-beta-D-xylopyranoside (7), 5'-methoxy-isolariciresinol-9'-O-beta-D-xylopyranoside (8), lyoniresinol-9'-O-beta-D-xylopyranoside (9), (2R, 3R) -2, 3-dihydro-2-(4-hydroxy-3-methoxyphenyl)-7-methoxy-3-methyl-5-(E)-propenylbenzofuran (10), 3, 5'-dimethoxy-4', 7-epoxy-8, 3'-neolignan-4, 9, 9'-triol (11), nectandrin B (12), and three flavanes(+)-catechin (13), (-)-epicatechin (14), and bis-8, 8'-catechinylmethane (15). All of the compounds 1-15 were separated from M. yaoshansis for the first time.
Butylene Glycols
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chemistry
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Catechin
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chemistry
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Lauraceae
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chemistry
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Lignans
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chemistry
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Lignin
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chemistry
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Naphthols
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chemistry
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Plant Roots
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chemistry
4.Enzymes related with NAD synthesis promote conversion of 1,4-butanediol to 4-hydroxybutyrate.
Chinese Journal of Biotechnology 2011;27(12):1749-1754
Besides medical application, 4-hydroxybutyrate (4-HB) is a precursor of P3HB4HB, a bioplastic showing excellent physical properties and degradability. Escherichia coli S17-1 (pZL-dhaT-aldD) can transform 1, 4-butanediol (1,4-BD) into 4HB with participation of cofactor NAD. To enhance productivity, nicotinic acid phosphoribosyltransferase (PncB) and nicotinamide adenine dinucleotide synthetase (NadE) were overexpressed to increase intracellular nicotinamide adenine dinucleotide concentration and promote reaction process. The shake flask fermentation result showed that the conversion rate increased by 13.03% with help of PncB-NadE, leading to 4.87 g/L 4HB from 10 g/L 1,4-BD, and productivity was increased by 40.91% to 1.86 g/g. These results demonstrated that expression of PncB and NadE is beneficial for conversion of 1,4-BD to 4HB.
Amide Synthases
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metabolism
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Butylene Glycols
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chemistry
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metabolism
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Escherichia coli
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metabolism
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Fermentation
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Hydroxybutyrates
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chemistry
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metabolism
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Pentosyltransferases
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metabolism
5.Production of 2, 3-butanediol and succinic acid by Salinivibrio YS.
Yuansheng XUE ; Gulsimay AIBAIDULA ; Guoqiang CHEN
Chinese Journal of Biotechnology 2011;27(12):1742-1748
The production of 2, 3-butanediol and succinic acid by a moderate halophile under anaerobic condition was investigated. This halophile, termed Salinivibrio YS, was isolated from the solid samples collected from Aydingkol Lake. Based on the single factor experiment, the parameters and their values for the production were obtained. Then, the optimum values of these parameters by the orthogonal experiments were obtained: temperature, 33 degrees C; initial pH of fermentation, 8.0; the pH during fermentation, 7.0; the concentration of acetic acid was 3 g/L and NaC1 was 10 g/L. Finally, a 3-L fermentation based on these conditions was carried out. After 108 h of fermentation under anaerobic condition, 35.05 g/L of 2, 3-butanediol and 22.46 g/L of succinic acid were obtained. About 50% of total glucose conversion was achieved. The study on 2, 3-butanediol and succinic acid by a halophile under anaerobic condition will expand the applications of halophiles and open a new area of production of 2, 3-butanediol and succinic acid.
Anaerobiosis
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Butylene Glycols
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metabolism
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Fermentation
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Halobacteriales
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metabolism
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Succinic Acid
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metabolism
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Vibrionaceae
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metabolism
6.Effects of Cross-Linking Agents on the Stability of Human Acellular Dermal Matrix.
Nak Heon KANG ; Young Mook YUN ; Jong Seol WOO ; Jae Hyung AHN ; Jin Young KIM
Journal of the Korean Society of Plastic and Reconstructive Surgeons 2008;35(3):248-254
PURPOSE: Human acellular dermal matrix(ADM) is widely used in the treatment of congenital anomalies and soft tissue deficiencies. But it is rapidly degraded in the body and does not provide satisfactory results. There is a need to improve collagen fiber stability through various methods and ultimately regulate the speed of degradation. METHODS: The ADMs were added with various cross- linking agents called glutaraldehyde, dimethyl 3,3'-dithiobispropionimidate to produce cross-linked acellular dermal matrices. 1,4-butanediol diglycidyl ether solution was applied with a pH of 4.5 and 9.0, respectively. The stability of cross-linked dermal matrix was observed by measuring the shrinkage temperature and the degradation rates. The cross- and non-cross linked dermis were placed in the rat abdomen and obtained after 8, 12 and 16 weeks. RESULTS: The shrinkage temperature significantly increased and the degradation rate significantly decreased, compared to the control(p<0.05). All of cross- linked dermises were observed grossly in 16 weeks, but most of non-cross linked dermis were absorbed in 8 weeks. Histologically, the control group ADM was found to have been infiltrated with fibroblasts and most of dermal stroma were transformed into the host collagen fibers. However, infiltration of fibroblasts in the experiment was insignificant and the original collagen structure was intact. CONCLUSION: Collagen cross-linking increases the structural stability and decreases degradation of acellular dermis. Therefore, decrease in body absorption and increase in duration can be expected.
Abdomen
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Absorption
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Acellular Dermis
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Animals
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Butylene Glycols
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Collagen
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Dermis
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Ether, Ethyl
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Fibroblasts
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Glutaral
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Humans
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Hydrogen-Ion Concentration
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Imidoesters
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Rats
7.Effect of acetic acid, furfural and 5-hydroxymethylfurfural on production of 2,3-butanediol by Klebsiella oxytoca.
Jing WU ; Keke CHENG ; Wenying LI ; Jie FENG ; Jian'an ZHANG
Chinese Journal of Biotechnology 2013;29(3):350-357
To get the tolerability and consumption of Klebsiella oxytoca on major inhibitors in lignocelluloses hydrolysate, we studied the effect of acetic acid, furfural and 5-hydroxymethylfurfural on production of 2,3-butanediol by Klebsiella oxytoca. The metabolites of furfural and 5-hydroxymethylfurfural were measured. The results show that when acetic acid, furfural and 5-hydroxymethylfurfural was individually added, tolerance threshold for Klebsiella oxytoca was 30 g/L, 4 g/L and 5 g/L, respectively. Acetic acid was likely used as substrate to produce 2,3-butanediol. The yield of 2,3-butanediol increased when acetic acid concentration was lower than 30 g/L. In the fermentation, more than 70% 5-hydroxymethylfurfural was converted to 2,5-furandimethanol. All furfural and the rest of 5-hydroxymethylfurfural were metabolized by Klebsiella oxytoca. It showed that in the detoxification process of 2,3-butanediol production using lignocelluloses hydrolysate, furfural should be given priority to remove and a certain concentration of acetic acid is not need to removal.
Acetic Acid
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chemistry
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Butylene Glycols
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metabolism
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Fermentation
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Furaldehyde
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analogs & derivatives
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chemistry
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Klebsiella oxytoca
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metabolism
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Lignin
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chemistry
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metabolism
8.Comparison of 2,3-butanediol production by several strains and optimization of the fermentation medium.
Yuanquan SONG ; Ruchun WU ; Yunzhen XU ; Ming FAN ; Dehua LIU
Chinese Journal of Biotechnology 2011;27(3):489-492
Five Klebsiella pneumonia strains (including two strains whose genes for lactic acid were knocked out) were used to produce 2,3-butanediol, in which K. pneumonia HR521 LDH (gene for lactic acid was knocked out) was the best for the production, and then the fermentation medium was optimized by orthogonal design. The optimum compositions were as follows: glucose 90 g/L, (NH4)2HPO4 3 g/L, CLSP 6 g/L, sodium acetate 5 g/L, KCl 0.4 g/L, MgSO4 0.1 g/L, FeSO4 x 7H2O 0.02 g/L, MnSO4 0.01 g/L. Under the above conditions, final concentration of acetone and 2,3-butanediol could reach 37.46 g/L, 10 g/L higher than that under the initial conditions, the yield was 90.53% of the theory, and the productivity was 1.5 g/(L-h), and no lactic acid was detected, which could be benefit for the downstream processing and industrial application.
Butylene Glycols
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metabolism
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Culture Media
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chemistry
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Fermentation
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Gene Knockout Techniques
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Glucose
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metabolism
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Klebsiella pneumoniae
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classification
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genetics
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growth & development
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metabolism
9.Effects of pH and oxygen supply on production of 2,3-butanediol from biodiesel-derived glycerol by Bacillus amyloliquefaciens.
Taowei YANG ; Zhiming RAO ; Xian ZHANG ; Meijuan XU ; Zhenghong XU
Chinese Journal of Biotechnology 2013;29(12):1860-1864
Bacillus amyloliquefaciens B10-127 was used to produce 2,3-butanediol (2,3-BD) from residual glycerol obtained from biodiesel synthesis. Important variables for 2,3-BD fermentation, pH and dissolved oxygen, were studied. When pH was maintained constant, the yield of 2,3-BD was inhibited. The highest 2,3-BD yields were achieved by fermentation without any pH control with an optimized initial pH 6.5. Batch fermentative production of 2,3-BD by B. amyloliquefaciens was investigated using various oxygen supply methods by changing agitation speed. Based on the analysis of three kinetic parameters including specific cell growth rate (micro), specific glucose consumption rate (q(s)) and specific 2,3-BD formation rate (q(p)), a three-stage agitation speed control strategy was proposed, aimed at achieving high concentration, high yield and high productivity of 2,3-BD. Maximum concentration of 2,3-BD reached 38.1 g/L, with the productivity of 1.06 g/(L x h), which were 14.8% and 63.1% over the best results from constant agitation speeds. In a pulse fed-batch fermentation, 2,3-BD concentration and productivity were significantly improved to 71.2 g/L and 0.99 g/(L x h), respectively. To our knowledge, these results were the highest for 2,3-BD production from biodiesel-derived glycerol.
Bacillus
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classification
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metabolism
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Biofuels
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analysis
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Bioreactors
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Butylene Glycols
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metabolism
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Fermentation
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Glycerol
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metabolism
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Hydrogen-Ion Concentration
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Industrial Microbiology
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Oxygen
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analysis
10.Analysis of GHB and Its Precursors in Urine and Their Forensic Application.
Yan SHI ; Xiao-pei CUI ; Ping XIANG ; Bao-hua SHEN
Journal of Forensic Medicine 2015;31(3):200-203
OBJECTIVE:
To establish the method to analyze γ-hydroxybutyric acid (GHB) and its precursors 1,4-butanediol (1,4-BD) and gamma-butyrolactone (GBL) in urine through LC-MS/MS and provide evidence for related cases.
METHODS:
GHB-d6 and MOR-d3 were used as the internal standard. The urine sample was separated by LC after protein precipitation with methanol. The electrospray ion source was for ionization. Each compound was detected through multiple-reaction monitoring (MRM) mode.
RESULTS:
The limits of detection of GHB and its precursors 1,4-BD and GBL were 0.1, 0.1 and 2 μg/mL. The accuracy was 87.6%-98.1%. The intra-day and inter-day precisions were less than 15% and matrix effects were higher than 80%.
CONCLUSION
The method is high sensitive, simple, rapid, specific and with high reliability. This study has provided technical support and basic data for forensic cases involving GHB.
4-Butyrolactone/urine*
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Butylene Glycols/urine*
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Chromatography, Liquid
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Forensic Sciences
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Humans
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Hydroxybutyrates/urine*
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Mass Spectrometry
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Reproducibility of Results
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Tandem Mass Spectrometry