1.Primary Burkitt lymphoma of the seminal vesicle: a case report and review of the literature.
Jun OUYANG ; Hong-Lin YIN ; Zhen-Feng LU ; Hang-Bo ZHOU ; Xiao-Jun ZHOU
National Journal of Andrology 2009;15(8):733-737
OBJECTIVETo investigate the clinicopathological features of primary Burkitt lymphoma of the seminal vesicle.
METHODSWe reported the clinical characteristics, histological changes and the results of immunohistochemical staining and molecular in situ hybridization of 1 case of primary Burkitt lymphoma of the seminal vesicle. We also reviewed the related literature and studied the pathomorphological characteristics and differential diagnosis of the tumor.
RESULTSThe characteristic manifestations of the patient were frequent micturition with dysuria, followed by inguinal lymphadenectasis 2 months later. Medical imaging showed a diffuse and monotonous infiltration of neoplastic cells with scanty cytoplasm and a few mitosis images. Microscopy displayed a starry sky pattern. The tumor cells were positive for CD10, CD20, CD79alpha, Bcl-6 and EBER in situ hybridization, but negative for CD3, CD6 and Cyclin D1. The Ki-67 index was > 95%.
CONCLUSIONPrimary Burkitt lymphoma of the seminal vesicle is a very rare tumor with aggressive behavior. The pathological diagnosis of the tumor depends on histopathological examination and immunohistochemical techniques. However it should be differentiated from diffuse large B-cell lymphoma, lymphoblastic lymphoma and small cell carcinoma of the seminal vesicle or prostate gland.
Burkitt Lymphoma ; diagnosis ; pathology ; Diagnosis, Differential ; Genital Neoplasms, Male ; Humans ; Male ; Middle Aged ; Seminal Vesicles ; pathology
2.Prognostic significance of lymphocyte function associated anti-gen-3 (CD58) in childhood B cell-acute lymphocytic leukemia.
Chong XU ; Hui-Jun ZHAO ; Li-Ming JIANG ; Xiao-Jun YUAN ; Li LI ; Jing-Yan TANG ; Li-Song SHEN
Journal of Experimental Hematology 2006;14(4):717-721
This study was aimed to investigate the value of CD58 in evaluation of early therapeutic effect on childhood B-ALL. The expression features of CD58 in 135 cases of childhood B-ALL were analyzed by four-color flow cytometry; MRD detection protocol for B-ALL using CD58/CD10/CD34/CD19 combination was established; the correlation between the expression features of CD58 and MRD detection was analyzed for the early therapeutic response in childhood B-ALL. The results showed that the mean value of CD58 MFI in 135 cases of B-ALL was 113.08 +/- 63.33, which was significantly higher than that in 15 cases of normal bone marrow controls (14.68 +/- 5.26, P < 0.01). In addition, CD58 was over expressed in 51.9% (70/135) of B-ALL patients, indicating that CD58 could be an effective marker in MRD detection. The CD58/CD10/CD34/CD19 was the second most effective combination next to TdT/CD10/CD34/CD19 in B-ALL MRD detection with flow cytometry. Meanwhile, the positive rate of MRD detection by flow cytometry was significantly lower in CD58 over expression group (P < 0.05). It is concluded that CD58 may be used as an indicator for detection of MRD in B-ALL patients, which would enrich the combination of MRD detection. The CD58 over expression may be considered as a marker of a favorable prognosis in childhood B-ALL.
Biomarkers, Tumor
;
analysis
;
Burkitt Lymphoma
;
immunology
;
pathology
;
CD58 Antigens
;
analysis
;
Child
;
Humans
;
Neoplasm, Residual
;
Prognosis
3.Genetic analysis of two cases with MYC "negative" Burkitt lymphoma.
Rui LYU ; Yingchun ZHENG ; Gang AN ; Chengwen LI
Chinese Journal of Medical Genetics 2023;40(11):1340-1344
OBJECTIVE:
To carry out combined genetic analysis on two patients suspected for Burkitt lymphoma to facilitate their diagnosis and treatment.
METHODS:
G banded karyotyping and interphase and metaphase fluorescence in situ hybridization (FISH) were used to detect the specific sites of chromosomes by using separate and fusion probes.
RESULTS:
The separate probe showed no presence of MYC gene abnormality, while fusion probe confirmed the IGH::MYC translocation in the samples. Combined with the clinical features and pathological characteristics, the two patients were finally diagnosed with Burkitt lymphoma, which was confirmed by targeted capture next generation sequencing.
CONCLUSION
The separate probe for the MYC gene has some shortcomings and should be used together with dual fusion probe to improve the accuracy of diagnosis.
Humans
;
Burkitt Lymphoma/pathology*
;
In Situ Hybridization, Fluorescence
;
Genes, myc
;
Translocation, Genetic
;
Karyotyping
4.Clinical manifestation of lymphoma and its significance in pathological diagnosis.
Xiao-ge ZHOU ; Jian-lan XIE ; Yan JIN ; Yuan-yuan ZHENG
Chinese Journal of Pathology 2012;41(1):57-58
Burkitt Lymphoma
;
diagnosis
;
pathology
;
Female
;
Hodgkin Disease
;
diagnosis
;
pathology
;
Humans
;
Lymphoma
;
classification
;
diagnosis
;
pathology
;
Lymphoma, Extranodal NK-T-Cell
;
diagnosis
;
pathology
;
Lymphoma, Follicular
;
diagnosis
;
pathology
;
Lymphoma, Large B-Cell, Diffuse
;
diagnosis
;
pathology
;
Male
;
Precursor Cell Lymphoblastic Leukemia-Lymphoma
;
diagnosis
;
pathology
5.Lymphotic and hematological diseases pathology in China.
Chinese Journal of Pathology 2005;34(8):499-501
6.Burkitt's Lymphoma Representing Periportal Infiltrating Mass on CT.
Kae Young PARK ; Jeong Sik YU ; Sang Wook YOON ; Mi Sook PARK ; Ja Seung KOO ; Ki Whang KIM
Yonsei Medical Journal 2004;45(4):723-726
Imaging findings of secondary hepatic lymphoma have been reported as variable, ranging from single or multiple small nodules to diffuse infiltrative tumor patterns. We hear present a rare case report concerning aggressive B cells, secondary Burkitt's lymphoma in non-AIDS demonstrating a surprising periportal lymphoma infiltration, without upper abdominal lymphadenopathy or splenomegaly on the sonography and CT scans. Clinically, the case was characterized by atypical and highly aggressive course, with the patient presenting an abruptly developed obstructive jaundice with rapidly deteriorating hepatic function that could be indicative of cholestatic hepatitis, which differs in its clinical manifestations from hepatic lymphoma without functional deterioration in respect of its non-tissue destructive growth pattern. We suggest that hepatic lymphoma can sometimes be consistent with periportal infiltrating homogeneous mass, with no lymphadenopathy or splenomegaly on the imaging examination, with a predictable aggressive clinical course of the disease and poor prognosis.
Abdomen
;
Burkitt Lymphoma/*pathology/ultrasonography
;
Fatal Outcome
;
Female
;
Humans
;
Liver Neoplasms/*pathology/ultrasonography
;
Middle Aged
;
Prognosis
;
*Tomography, X-Ray Computed
8.Detection of B lymphoma cells undergoing apoptosis by Annexin-V assay.
Liming PENG ; Hong JIANG ; Chris BRADELY
Chinese Medical Sciences Journal 2002;17(1):17-21
OBJECTIVETo quantitatively analyze apoptotic and secondary necrotic cells under apoptosis conditions.
METHODSThe cells of Burkitt lymphoma (BL) cell line Raji were incubated with 1.0 micromol/L dexamethasone (DEX) for 2, 4 and 8 h respectively, then stained with Annexin V-FITC (fluorescein isothiocyanate conjugated) which was used to detect the exposed phosphatidylserine (PS) on the epimembrane resulting from a loss of phospholipid asymmetry in the early stage of apoptosis, and also stained with propidium iodide (PI) which allows analysis of secondary necrotic cells related with cell membrane and DNA damage that probably representlate stage of apoptosis, then apoptotic cells were quantified by flow cytometry (FCM). Furthermore, Annexin+ /PI- and Annexin+ /PI+ cells were sorted by fluoresence-activated cell sorter (FACS), and identified by electron microscopy (EM) and DNA gel electrophoresis.
RESULTSThe percentage of apoptotic cells was found to increase with the incubation time (r = 0.97). This method was sensitive with low detection limit (0.02%), and was reproducible with low coefficient variance (CV) (4.2%). Meanwhile, the Annexin+ /PI- and Annexin+ /PI+ cells were identified as apoptotic and necrotic cells under EM, and DNA extracted from the Annexin+ /PI- cells was characterized by "ladder pattern".
CONCLUSIONSAnnexin-V assay is a specific, sensitive, accurate, reproductive and quantitative method for analyzing apoptotic cells.
Annexin A5 ; analysis ; Apoptosis ; Burkitt Lymphoma ; pathology ; DNA Damage ; Humans ; Necrosis ; Phosphatidylserines ; metabolism ; Propidium ; analysis ; Tumor Cells, Cultured
9.Abdominal Burkitt's lymphoma diagnosed by fine needle aspiration cytology: a case report.
Na Hye MYONG ; Kyung Ja CHO ; Sang Wook CHOI ; Ja June JANG
Journal of Korean Medical Science 1990;5(2):97-99
A case of abdominal Burkitt's lymphoma diagnosed through aspiration cytology is described. This 9-year-old boy presented with abdominal pain and distention for three months accompanied by fever and night sweat during the last month. An abdominal sonography and CT scan showed hepatosplenomegaly and an intrahepatic mass with celiac lymph node enlargement, ascites, and pleural fluid. A peripheral blood smear showed a few blast cells. Aspiration of the abdominal mass revealed very cellular aspirates consisting of diffusely scattered small monotonous round cells. The cells had little cytoplasm, along with round nuclei that showed clear-cut nuclear membrane, coarse chromatin pattern, and multiple small prominent nucleoli. Differential diagnoses considered were small round cell sarcomas such as malignant lymphoma, neuroblastoma, Ewing's sarcoma, and rhabdomyosarcoma. Of these, malignant lymphoma of the small noncleaved cell type was most consistent with the results of several studies including immunohistochemical staining, peripheral blood smear, and bone marrow biopsy. The cells were positive for leukocyte common antigen (LCA) and showed finely vacuolated basophilic cytoplasm in both the peripheral blood smear and bone marrow biopsy, characteristic of Burkitt's lymphoma cells.
Biopsy, Needle
;
Burkitt Lymphoma/*diagnosis/pathology/radionuclide imaging
;
Child
;
Diagnosis, Differential
;
Humans
;
Male
;
Neuroblastoma/*diagnosis/pathology
;
Rhabdomyosarcoma/*diagnosis/pathology
;
Sarcoma, Ewing/*diagnosis/pathology
;
Tomography, X-Ray Computed
10.Pathological diagnosis of pediatric Burkitt lymphoma involving bone marrow.
Qi SUN ; Zhenping CHEN ; Enbin LIU ; Zhanqi LI ; Qingying YANG ; Fujun SUN ; Yue MA ; Hongju ZHANG ; Peihong ZHANG ; Kun RU
Chinese Journal of Pathology 2015;44(2):95-99
OBJECTIVETo investigate pathologic and differential diagnostic features of pediatric Burkitt lymphoma (BL).
METHODSA total of 20 cases of pediatric BL were retrospectively reviewed for their clinical and pathologic profiles. Bone marrow aspiration specimens were available in all cases and bone marrow biopsies were available for immunohistochemical study in 18 cases. Flow cytometry study was available in 16 cases. MYC translocation by FISH method was performed in 11 cases.
RESULTSAtypical lymphocytes with cytoplasmic vacuoles were found in bone marrow smears in all 20 cases and peripheral blood films in all 19 available cases. The bone marrow biopsies showed infiltration by uniform medium-sized atypical lymphocytes with multiple small nucleoli but without the starry-sky pattern in all 18 cases. Immunohistochemistry showed the following results in all 18 cases: positive for CD20, PAX-5, CD10, CD34 and TdT, but negative for bcl-2 and CD3 with Ki-67 > 95%.Flow cytometry showed CD19+CD20+CD10+FMC7+CD22+TdT-CD3- in 16 cases, including κ+ in 8 cases, λ+ in 7 cases, and κ-λ- in 1 case. MYC gene rearrangement by FISH was observed in 10 of the 11 cases.
CONCLUSIONSThe histopathology of BL is distinct, including atypical lymphocytes with cytoplasmic vacuoles in bone marrow aspirate, lack of starry-sky patternin bone marrow biopsy. Generally, the diagnosis should be made with a combined immunophenotype and FISH approach. Pediatric BL must be distinguished from DLBCL and B-cell lymphoma, unclassifiable, which has intermediate features between DLBCL and Burkitt lymphoma.
Biopsy ; Bone Marrow ; pathology ; Burkitt Lymphoma ; genetics ; pathology ; Child ; Diagnosis, Differential ; Female ; Flow Cytometry ; Genes, myc ; Humans ; Immunohistochemistry ; Immunophenotyping ; In Situ Hybridization, Fluorescence ; Lymphocytes ; pathology ; Lymphoma, B-Cell ; pathology ; Lymphoma, Large B-Cell, Diffuse ; pathology ; Male ; Retrospective Studies ; Translocation, Genetic