No abstract available.

No abstract available.
Animals ; Dogs* ; Lung*

Traumatic brain injury (TBI) is one of the leading causes of death in the pediatric population in Korea. In addition, it can cause disability in children and adolescents, with physical and mental consequences. This causes a substantial burden on the health care system and occurs globally and not just in Korea. We searched and reviewed current data on the epidemiologic characteristics of pediatric TBI in Korea. Our review provides the recent epidemiological trend mainly focusing on incidence and mortality along with worldwide reported data. This review will be helpful to understand the global epidemiology of pediatric TBI and its differences between countries.

The aim of this investigation was to determine the incidence of sperm antibodies in serum using indirect immunobead test (IBT) in 113 patients of scrotal disease. The results of this survey showed that 17.7% of 113 men had antisperm antibodies of IgG and/or IgA class bound to the surface of at least 20% of motile spermatozoa of normal donor. There were positive immunobead test in 16 of 36(44.4% ) vasovasostomy patients, 1 of 18 testicular trauma, 1 of 15 primary infertility, 1 of 4 obstructive infertility, 1 of 7 hydrocele. There were no statistical differences in sperm count, sperm morphology between the groups of patients with positive or negative IBT results. As for the immunoglobulins, the most frequent class was IgG, and in terms of morphological sites, tail binding was most frequent. The results of this investigation therefore suggest that the IBT is an excellent test for sperm antibodies in scrotal disease.
Antibodies* ; Humans ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulins ; Incidence ; Infertility ; Male ; Sperm Count ; Spermatozoa ; Tissue Donors ; Vasovasostomy

One of the purposes of this experiment is to observe the structure of crystalline lens with cataract, which is formed artificially, using the light microscope and electron microscope. The other is to observe the differences of structural variations in the cataract developed inside body. Twelve eyes of six pigs were used for this experiment. Two of them are normal crystalline lens, five are cystalline lens in distilled water, and the rest are in balanced salt solution through intact or ruptured capsule. We examined the time of cataract formation and compared the ultrastructural changes. Ruptured capsule and high osmotic pressure difference induced more rapid opacity. In case of distilled water, the capsule is maintained but it is very difficult to distinguish between epithelium and lens fibers. Also, there is a severe crack in the lens fibers. In electron microscopic, as the cataract progresses, the osmotic swelling becomes more prominent. One of the striking changes was a marked intercellular cyst formation. Lens cells became irregular in size and density and were extensively vacuolated. The swelling of the lens cells continuously induced large intracellular vacuoles and liquefied the cytoplasmic protein. In conclusion, the structural change of cataract, which was seen by an electron microscope, resulted from change in osmolarity from previously announced in vivo experiment and structural change resulted from this experiment are similar.
Cataract* ; Crystallins* ; Cytoplasm ; Epithelium ; Lens, Crystalline* ; Microscopy, Electron ; Osmolar Concentration ; Osmotic Pressure ; Strikes, Employee ; Swine ; Vacuoles ; Water

BACKGROUND AND OBJECTIVES: Allergic rhinitis (AR) and rhinitis medicamentosa (RM) have different mucosal color and pathophysiology. To investigate whether the mucosal color and nasal blood flow are different between the diseases in spite of same symptoms, we designed this study. Materials and Methods: 20 patients with allergic rhinitis and 21 patients with rhinitis medicamentosa were compared with 20 normal volunteers using mucosal color grading and Laser Doppler flowmetry. The Laser Doppler flowmetry was performed with a Periflux 4001 (Perimed, Jrtlla, Sweden) and perfusion unit (PU), velocity unit (VU), and concentration Unit (CU) were measured. The Laser Doppler flowmetry data in AR and RM were compared with those of the normal subjects, and between AR and RM. RESULTS: The perfusion score of AR and RM were lower than the control (p<0.05) and it was statistically significant that the mucosal color of AR were pale and of RM were reddish, comparing to the control group (p<0.05). CONCLUSION: The nasal blood flow was decreased with AR and RM compared to control but the mucosal color of AR and RM were different because of the difference of pathophysiology of diseases. When diagnosing RM, observation of mucosal color and measurement of nasal blood flow will be helpful besides the history of long-term use of nasal decongestant.
Healthy Volunteers ; Humans ; Laser-Doppler Flowmetry* ; Perfusion ; Rhinitis*

Gliomas are the most common pediatric tumors of the central nervous system. In this review, we discuss the clinical features, treatment paradigms, and evolving concepts related to two types of pediatric gliomas affecting two main locations: the optic pathway and thalamus. In particular, we discuss recently revised pathologic classification, which adopting molecular parameter. We believe that our review contribute to the readers' better understanding of pediatric glioma because pediatric glioma differs in many ways from adult glioma according to the newest advances in molecular characterization of this tumor. A better understanding of current and evolving issues in pediatric glioma is needed to ensure effective management decision.
Adult ; Brain Neoplasms ; Central Nervous System ; Classification ; Glioma ; Humans ; Optic Tract ; Pediatrics ; Thalamus ; Treatment Outcome

We expressed anti-HBsAg human antibody fragment (B7 Fd) using pRSET-A vector and BL21(DE3)pLysS strain of E. coli. B7 Fd is composed only of variable domain (VH) and CH1 constant domain of IgG heavy chain molecule. This Fd molecule was solubilized using guanidine salt and then expressed in the form of inclusion body and successfully refolded into functional antibody molecule by rapid dilution in refolding buffer. B7 Fd reacted with d epitope of hepatitis B virus surface antigen (HBsAg). Its affinity was determined by competition enzyme-linked immunosorbent assay (competition ELISA). The K value of B7 Fd was 3.3 * 10.
Antigens, Surface ; Enzyme-Linked Immunosorbent Assay ; Guanidine ; Hepatitis B virus ; Humans* ; Immunoglobulin G ; Inclusion Bodies

BACKGROUND: Numerous studies of safe, long term preservation for lung transplantation have been performed using ex vivo models or in vivo single lung transplantation models. However, a safe preservation time which is applicable for clinical use is difficult to determine. We prepared LPDG solution for lung preservation study. In this study we examined the efficacy of LPDG(low potassium dextran glucose) solution in 24-hour lung preservation by using a sequential bilateral canine lung allotransplant model. MATERIAL AND METHOD: Seven bilateral lung transplant procedures were performed using weight-matched pairs(24 to 25kg) of adult mongrel dogs. The donor lungs were flushed with LPDG solution and maintained hyperinflated with 100% oxygen at 10C degrees for a planned ischemic time of 24 hours for the lung implanted first. After sequential bilateral lung transplantation, dogs were maintained on ventilators for 3 hours: arterial resistance were determined if the recipients hourly after bilateral reperfusion and compared with pretransplant-recipient values, which were used as controls. After 2hours of reperfusion, the chest X-ray, computed tomogram and lung perfusion scan were performed for assessmint of early graft lung function. Pathological examinations for ultrastructural findings of alveolar structure and endothelial structure of pulmonary artery were performed. RESULT: Five of seven experiments successfully finished the whole assessments after bilateral reperfusion for three hours. Arterial oxygen tension in the recipients was markedly decrased in immediate reperfusion period but gradually recovered after reperfusion for three hours. The pulmonary artery and pulmonary vascular resistance showed singificant elevation(p<0.05 versus control values) but also recovered after reperfusion for three hours(p<0.05 versus immediate period value). The ultrastructural findings of alveolar structure and endothelial structure of pulmonary artery showed reversible mild injury in 24 hours of lung perservation and reperfusion. CONCLUSION: This study suggests that LPDG solution provides excellent preservation in a canine model in which the dog is completely dependent on the function of the transplanted lung.
Adult ; Animals ; Dextrans ; Dogs ; Humans ; Lung Transplantation ; Lung* ; Organ Preservation ; Oxygen ; Perfusion ; Potassium ; Pulmonary Artery ; Reperfusion ; Thorax ; Tissue Donors ; Transplants ; Vascular Resistance ; Ventilators, Mechanical

BACKGROUNDS: The aim of this retrospective study was to compare patients treated for complicated peptic ulcers with special reference to the morbidity and the mortality rates and its determining factors according to the type of surgery. METHODS: From Jan. 1988 to May 1996, a total of 168 patients with complicated peptic ulcers underwent surgical treatment. Among them, 39 patients were lost during the follow up period and 10 cases were operated on due to pyloric obstruction, so we analyzed 119 cases according to the type of surgery (definitive or minimal). Definitive surgery means acid-decreasing procedures such as a gastrectomy or a truncal vagotomy with drainage procedure. Minimal surgery means life-saving procedures such as primary closure or bleeding vessel ligation. The type of operation was determined according to the preference of the individual consultant surgeons at our institute. RESULTS: There were 70 definitive and 49 minimal operations (6:4). The mean follow-up period was 20.6 months (range: 11-73 months). The risk factors affecting the morbidity and the mortality were comparable for both groups. In spite of little statistical significance, the results of definitive surgery were superior to those of minimal surgery in morbidity (15.7%:20.4%), mortality (0%:6.1%), recurrence (5.5%:12.2%), and Visick grading scale 1 & 2 (92.8%:81.6%). The determining factor affecting morbidity was the presence of combined disease (p=0.001) only; other factors such as age, anemia, leukocytosis, presence of preoperative shock, transfusion amount, and type of operation did not affect the morbidity rate. CONCLUSION: Considering that there were no differences in postoperative morbidity and mortality between definitive and minimal surgery, it is better to perform definitive surgery on patients who have no serious combined disease.
Anemia ; Consultants ; Drainage ; Follow-Up Studies ; Gastrectomy ; Hemorrhage ; Humans ; Leukocytosis ; Ligation ; Mortality ; Peptic Ulcer* ; Recurrence ; Retrospective Studies ; Risk Factors ; Shock ; Vagotomy, Truncal