1.Prevalence of Zoonotic Metacercariae in Two Species of Grouper, Epinephelus coioides and Epinephelus bleekeri, and Flathead Mullet, Mugil cephalus, in Vietnam.
Dung The VO ; Darwin MURRELL ; Anders DALSGAARD ; Glenn BRISTOW ; Dung Huu NGUYEN ; Thanh Ngoc BUI ; Dung Thi VO
The Korean Journal of Parasitology 2008;46(2):77-82
Fishborne zoonotic metacercariae have not been reported from brackish water and marine fish from Vietnam waters although these parasites are common in the country's freshwater fish. Both wild-caught and cultured grouper (Epinephelus coioides and Epinephelus bleekeri), and mullet (Mugil cephalus) from brackish and marine waters locate in Khanh Hoa province in central coastal Vietnam were examined, and found positive for zoonotic trematode metacercariae. From grouper, Heterophyopsis continua and Procerovum varium were recovered. The prevalence of H. continua ranged from 2.0 to 6.0% and that for P. varium ranged from 11.6 to 15.8%. Mullet were infected with Pygidiopsis summa and H. continua both of these species are new records for Vietnam. The prevalence of P. summa in mullet was generally high, ranging from 17.6 to 75.5%, and was significantly higher than the prevalence of H. continua (2.5 to 32.4%). There were no significant differences in the prevalence of metacercariae between grouper from natural or cultured habitats, indicating that the highest risk of infection occurs in the wild-caught state prior to their placement in culture. Further, there was no difference in metacercarial prevalence between the 2 species of grouper. Infected wild-caught seed were only observed from January to October. Monthly variation in prevalence suggests seasonal variation in mullet infections occurs in this region with the highest transmission taking place from October to December. Basic investigations on the ecology and epidemiology of these intestinal flukes need to be carried out to determine their significance as a public health problem and the aspects of their biology that may be vulnerable to control interventions.
Animals
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Bass/*parasitology
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Fish Diseases/*epidemiology/parasitology
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Fresh Water/parasitology
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Humans
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Prevalence
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Seawater/parasitology
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Smegmamorpha/*parasitology
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Time Factors
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Trematoda/*isolation & purification
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Trematode Infections/epidemiology/parasitology/*veterinary
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Vietnam/epidemiology
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Zoonoses/*epidemiology/parasitology
2.Morphological and Molecular Characterization of Lymnaeid Snails and Their Potential Role in Transmission of Fasciola spp. in Vietnam.
Bui Thi DUNG ; Pham Ngoc DOANH ; Dang Tat THE ; Ho Thi LOAN ; Bertrand LOSSON ; Yannick CARON
The Korean Journal of Parasitology 2013;51(6):657-662
Freshwater snails of the family Lymnaeidae play an important role in the transmission of fascioliasis worldwide. In Vietnam, 2 common lymnaeid species, Lymnaea swinhoei and Lymnaea viridis, can be recognized on the basis of morphology, and a third species, Lymnaea sp., is known to exist. Recent studies have raised controversy about their role in transmission of Fasciola spp. because of confusion in identification of the snail hosts. The aim of this study is, therefore, to clarify the identities of lymnaeid snails in Vietnam by a combination of morphological and molecular approaches. The molecular analyses using the second internal transcribed spacer (ITS2) of the nuclear ribosomal DNA clearly showed that lymnaeids in Vietnam include 3 species, Austropeplea viridis (morphologically identified as L. viridis), Radix auricularia (morphologically identified as L. swinhoei) and Radix rubiginosa (morphologically identified as Lymnaea sp.). R. rubiginosa is a new record for Vietnam. Among them, only A. viridis was found to be infected with Fasciola spp. These results provide a new insight into lymnaeid snails in Vietnam. Identification of lymnaeid snails in Vietnam and their role in the liver fluke transmission should be further investigated.
Animals
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Cluster Analysis
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DNA, Ribosomal Spacer/chemistry/genetics
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Fasciola/*isolation & purification
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Lymnaea/*anatomy & histology/genetics/*parasitology
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Molecular Sequence Data
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Phylogeny
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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Vietnam
3. Down-regulated expression of NPM1 in IMS-M2 cell line by (-)-epigallocatechin-3-gallate
Hoang Thanh CHI ; Phu Chi DUNG ; Phan Thi XINH ; Bui Thi Kim LY ; Hoang Anh VU ; Phan Thi XINH ; Yuko SATO
Asian Pacific Journal of Tropical Biomedicine 2014;4(7):570-574
Objective: To investigate the inhibited effect of epigallocatechin-3-gallate (EGCG) on the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Methods: Cell proliferation assay was performed to test the effects of EGCG on cell growth of IMS-M2 cells harboring the NPM1 mutations. Western blot analysis were performed to test the protein expression of NPM1, AKT, those associated with apoptosis. Results: EGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Moreover, EGCG also suppressed the cell proliferation and induced apoptosis in IMS-M2 cells. Conclusions: The results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.
4.Down-regulated expression of NPM1 in IMS-M2 cell line by (-)-epigallocatechin-3-gallate.
Hoang Thanh CHI ; Bui Thi Kim LY ; Hoang Anh VU ; Yuko SATO ; Phu Chi DUNG ; Phan Thi XINH ;
Asian Pacific Journal of Tropical Biomedicine 2014;4(7):570-574
OBJECTIVETo investigate the inhibited effect of epigallocatechin-3-gallate (EGCG) on the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations.
METHODSCell proliferation assay was performed to test the effects of EGCG on cell growth of IMS-M2 cells harboring the NPM1 mutations. Western blot analysis were performed to test the protein expression of NPM1, AKT, those associated with apoptosis.
RESULTSEGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Moreover, EGCG also suppressed the cell proliferation and induced apoptosis in IMS-M2 cells.
CONCLUSIONSThe results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.