Objective To explore the effect of century cloud weight loss(CCWL)follow-up system on the dietary behavior compliance,follow-up rate and weight loss effect of patients after bariatric surgery.Methods A total of 222 obese patients undergoing Beijing Shijitan Hospital Affiliated to Capital Medical University in 2022 were selected as the study subjects.A total of 108 patients from January to June 2022 were included in control group,and routine follow-up management mode was adopted.A total of 114 patients from July to December 2022 were included in intervention group and were followed up by CCWL follow-up system.The dietary behavior compliance,postoperative follow-up rate,percentage of excess weight loss(%EWL),glucose and lipid metabolism indexes were compared between two groups.Results At 1 month,3 months and 6 months after surgery,the eating behavior after bariatric surgery(EBBS)score,follow-up rate and%EWL of patients in intervention group were significantly higher than those in control group(P<0.05).At 1 month after surgery,there was no significant difference in triacylglycerol(TG),high-density lipoprotein cholesterol(HDL-C),fasting blood glucose(FBG)levels in two groups(P>0.05),and 2-hour postprandial blood glucose(2hBG)in intervention group was significantly lower than that in control group(P<0.05).At 3 months and 6 months after surgery,TG,FBG and 2hBG in intervention group were significantly lower than those in control group,while HDL-C was significantly higher than that in control group(P<0.05).Conclusion The application of CCWL follow-up system in patients with bariatric surgery can improve postoperative eating behavior,increase postoperative follow-up rate,and strengthen the effect of postoperative weight loss.

RNA-binding proteins (RBPs) are ubiquitous components within cells, fulfilling essential functions in a myriad of biological processes. These proteins interact with RNA molecules to regulate gene expression at various levels, including transcription, splicing, transport, localization, translation, and degradation. Understanding the intricate network of RBP-RNA interactions is crucial for deciphering the complex regulatory mechanisms that govern cellular function and organismal development. Ultravidet (UV) cross-linking and immunoprecipitation (CLIP) stands out as a powerful approach designed to map the precise locations where RBPs bind to RNA. By using UV light to create covalent bonds between proteins and RNA, followed by immunoprecipitation to isolate the protein-RNA complexes, researchers can identify the direct targets of specific RBPs. The advent of high-throughput sequencing technologies has revolutionized CLIP, enabling the identification of not only the types but also the exact sequences of RNA bound by RBPs on a genome-wide scale. The evolution of CLIP has led to the development of specialized variants, each with unique features that address specific challenges and expand the scope of what can be studied. High-throughput sequencing CLIP (HITS-CLIP) was one of the first advancements, significantly increasing the throughput and resolution of RNA-protein interaction mapping. Photoactivatable-ribonucleoside-enhanced CLIP (PAR-CLIP) introduced the use of photoactivatable ribonucleosides to enhance cross-linking efficiency and specificity, reducing background noise and improving the detection of low-abundance RNA-protein interactions. Individual-nucleotide resolution CLIP (iCLIP) further refined the technique, achieving unprecedented precision by resolving individual nucleotides involved in RBP binding, which is particularly valuable for studying the fine details of RNA structure and function. Despite the remarkable progress, there remains room for improvement in CLIP technology. Researchers continue to seek methods to increase sensitivity, reduce technical variability, and improve the reproducibility of results. Advances in sample preparation, data analysis algorithms, and computational tools are critical for addressing these challenges. Moreover, the application of CLIP to more diverse biological systems, including non-model organisms and clinical samples, requires the development of tailored protocols and the optimization of existing ones. Looking forward, the field of RNA biology is poised to benefit greatly from ongoing innovations in CLIP technology. The exploration of non-canonical RNA-protein interactions, such as those involving long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), promises to reveal new layers of cellular regulation and may lead to the discovery of novel therapeutic targets. Furthermore, integrating CLIP data with other omics approaches, such as proteomics and metabolomics, will provide a more comprehensive understanding of the dynamic interplay between RNA and its binding partners within the cell. In conclusion, the continuous refinement and expansion of CLIP techniques have not only deepened our knowledge of RNA biology but have also opened up new avenues for investigating the molecular underpinnings of health and disease. As the technology matures, it is expected to play an increasingly pivotal role in both basic and applied research, contributing to the advancement of medical science and biotechnology.