1.Processing evolution,chemical components and pharmacological effects of Bufonis Venenum.
Jin-Qin YING ; Ming YANG ; Pu-Zhao ZHANG ; Jin-Lian ZHANG
China Journal of Chinese Materia Medica 2021;46(14):3529-3539
Bufonis Venenum,the dried secretion of Bufo bufo gargarizans or B. melanostictus,is toxic and hard with the efficacy of removing toxicity for detumescence and relieving pain. The processing of Bufonis Venenum dates back to the Song dynasty. In addition to the wine-processing,milk-processing and talcum powder-processing,there were some other kinds of processing methods in ancient times,such as baking,calcining,water-soaking and vinegar-processing. Modern studies have shown that the Bufonis Venenum has the main chemical components of bufadienolides,indole alkaloids sterols,and other compounds. It has the pharmacological effects of antitumor,cardiac,antibacterial,and analgesic activities,local anesthesia,and so on. This paper reviews the processing evolution,chemical components and pharmacological effects of Bufonis Venenum,providing references for its special processing and modern research as well as the theoretical basis for the research on its processing mechanism and quality control.
Animals
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Bufanolides/pharmacology*
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Bufonidae
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Quality Control
3.Chemical constituents of bufadienolides in cinobufacino injection.
Ling-Yu HAN ; Nan SI ; Jun-Qiu LIU ; Hai-Yu ZHAO ; Jian YANG ; Bao-Lin BIAN ; Hong-Jie WANG
Acta Pharmaceutica Sinica 2014;49(11):1574-1577
Cinobufacino injection is purified from water extraction of the skin of Bufo bufo gargarizans, which has been widely used for various cancers in clinic with significant anti-tumor effects. Bufadienolides were regarded as the main active constituents of cinobufacino injection in previous reports. In present study, 6 bufadienolides were isolated and purified from Cinobufacino injection. Their structures were identified as 3-epi-ψ-bufarenogin (1), ψ-bufarenogin (2), 3-epi-arenobufagin (3), arenobufagin (4), 3-epi-gamabufotalin (5), and 3-oxo-arenobufagin (6), separately. Among them, 1 and 3 were new compounds, 5 and 6 were new natural products. Compounds 1, 2 and compounds 3, 4 were two pairs configuration isomers at C-3, separately.
Animals
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Bufanolides
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chemistry
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isolation & purification
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Bufo bufo
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Injections
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Skin
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chemistry
4.Determination of apparent aqueous solubility and apparent oil-water partition coefficients of three bufadienolides composition.
China Journal of Chinese Materia Medica 2008;33(11):1256-1258
OBJECTIVETo investigate apparent aqueous solubility and apparent oil-water partition coefficients of three bufadienolides composition.
METHODA high performance liquid chromatography method was established to determine apparent aqueous solubility of three bufadienolides composition; apparent oil-water partition coefficients of three bufadienolides composition was determined by shaking flask method.
RESULTThe solubility of three bufadienolides composition reached the top value, 76.29 microg x mL(-1) (RBG), 51.85 microg x mL(-1) (CBG), 32.76 microg x mL(-1) (BL) respectively and a total of 160.9 microg x mL(-1) in the condition of 37 degrees C and pH 7.0, therefore three bufadienolides composition can be defined as insoluble products. The solubility of the three decreased in weak acid or base environment to some extent. RBG, CBG, BL are liposoluble components and the sequence of log P is RBG > BL > CBG.
CONCLUSIONRBG, CBG, BL is water-insoluble and hydrophobic. Surfactants can be applied to increase the solubility of three bufadienolides composition.
Bufanolides ; chemistry ; Calibration ; Hydrogen-Ion Concentration ; Logistic Models ; Oils ; chemistry ; Solubility ; Temperature ; Water ; chemistry
5.Effect of cinobufacini injection on the tumor growth of tumor bearing rats of different constitutions.
Shuang-shuang WANG ; Xiao-feng ZHAI ; Bai LI
Chinese Journal of Integrated Traditional and Western Medicine 2011;31(8):1101-1103
OBJECTIVETo observe effects of cold- and hot-property herbs and effects of hot and cold constitutions on the tumor growth of tumor bearing rats, and to observe the effect of Cinobufacini Injection (CI) on the tumor growth of tumor bearing rats of different constitutions.
METHODSEighty healthy male Wistar rats were randomly divided into eight groups, i.e., the tumor bearing control group, the tumor bearing heat syndrome group, the tumor bearing cold syndrome group, the heat syndrome tumor bearing group, the cold syndrome tumor bearing group, the tumor bearing CI group, the heat syndrome tumor bearing CI group, and the cold syndrome tumor bearing CI group, respectively. The weight and volume of rats' subcutaneous tumor were measured 14 days after tumor inoculation.
RESULTSThe weight and volume of tumor in the heat syndrome tumor bearing CI group [(3.55 +/- 1.12) g, (2864.44 +/- 1430.51) mm3] and the tumor bearing CI group [(4.29 +/- 1.14) g, (3397.19 +/- 1701.13) mm3] were significantly lower than those of the tumor bearing control group [(6.01 +/- 2.45) g, (6218.91 +/- 3837.64) mm3] and the cold syndrome tumor bearing CI group [(6.90 +/- 1.57) g, (6168.42 +/- 2457.03) mm3], showing statistical difference (P<0.05). There was insignificant difference among other groups.
CONCLUSIONSCI showed better tumor inhibition effects on tumor bearing rats of heat syndrome constitution, which indicated CI was of cold property. It might be possibly used in tumor bearing rats of heat syndrome constitution.
Animals ; Bufanolides ; pharmacology ; therapeutic use ; Injections ; Male ; Neoplasms, Experimental ; drug therapy ; pathology ; Rats ; Rats, Wistar
6.Comparison of the chemical composition between fresh and dried venenum bufonis by UPLC-Orbitrap MS.
Jing HE ; Yan LI ; Nan SI ; Hai-Yu ZHAO ; Bao-Lin BIAN ; Hong-Jie WANG
Acta Pharmaceutica Sinica 2014;49(10):1446-1450
To identify the active components in Bufo melanostictus Schneider and clarify the difference between fresh and dried Venenum Bufonis, a UPLC-Orbitrap MS method has been established. The separation was performed with gradient elution of acetonitrile and water (with 0.1% formic acid) as mobile phase. By comparing their retention time and high resolution mass data of Venenum Bufonis extracts, 39 effective components were primarily identified by MS/MS analysis in positive ion mode. Twenty-six of them were bufadienolides. There were significant differences in the main composition between fresh and dried Venenum Bufonis. There are fewer bufadienolides in fresh toad venom.
Amphibian Venoms
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chemistry
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Animals
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Bufanolides
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chemistry
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Bufonidae
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Chromatography, High Pressure Liquid
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Tandem Mass Spectrometry
7.Determination of plasma protein binding rate of bufalin.
Ying LIU ; Zhiqiang CHEN ; Beilei TAO ; Nianping FENG ; Jihui ZHAO
China Journal of Chinese Materia Medica 2009;34(21):2817-2820
OBJECTIVETo study plasma protein binding rate of bufalin.
METHODHPLC was employed to determine the concentration of bufalin. Plasma protein binding rate studies were conducted by equilibrium dialysis method. The influence of drug concentration and plasma in different species on plasma protein binding rate were studied.
RESULTThere was no significant difference in the plasma protein binding rates at low, middle and high bufalin concentrations in dilution medium. The protein binding rate of bufalin in human plasma was higher than in the rat plasma.
CONCLUSIONBufalin has higher protein binding extent with both rat plasma and human plasma.
Animals ; Blood Proteins ; chemistry ; Bufanolides ; chemistry ; Humans ; Kinetics ; Male ; Protein Binding ; Rats ; Rats, Sprague-Dawley
8.Study on quality standards for Lingmaoxiang Jiedu pills.
Cheng ZHENG ; Binbin ZHENG ; Tongwei YAO
China Journal of Chinese Materia Medica 2010;35(3):305-307
OBJECTIVETo establish the quality standards of Linmaoxiang Jiedu pills.
METHODCinobufagin and bufogenin were determined by HPLC simultaneously.
RESULTThe average recoverys of Cinobufagin and bufogenin was 100.5% and 100.3%, their linear range were 48.74-731.10 ng and 49.90-748.50 ng, respectively.
CONCLUSIONThe method for quantification is reproducible and realizable. The method can be used to control quality of Linmaoxiang Jiedu pills as the quality standards.
Bufanolides ; analysis ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; analysis ; Reproducibility of Results
9.Bufadienolides from venom of Bufo bufo gargarizans.
Peng-Wei ZHANG ; Ren-Wang JIANG ; Wen-Cai YE ; Hai-Yan TIAN
China Journal of Chinese Materia Medica 2014;39(5):841-845
Twelve compounds were isolated from the venom of Bufo bufo gargarizans. On the basis of their physical and chemical properties and spectral data, their structures were identified as resibufagenin (1), bufotalin (2), desacetylcinobufagin (3), 19-oxodesacetylcinobufotalin (4), cinobufotalin (5), 1beta-hydroxylbufalin (6), 12alpha-hydroxybufalin (7), bufotalinin (8), Hellebrigenin (9), telocinobufagin (10), hellebrigenol (11) and cinobufagin-3-hemisuberate methyl ester (12), respectively. Compounds 7 and 12 are new natural products.
Animals
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Bufanolides
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chemistry
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Bufo bufo
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Medicine, Chinese Traditional
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Molecular Structure
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Venoms
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chemistry
10.Anti-tumor effect and its related mechanisms of cinobufotalin combined with cisplatin on H22 liver cancer mice.
Pei-Pei WANG ; Yong-Hui WANG ; Li-Sen WANG ; Tao WU
China Journal of Chinese Materia Medica 2020;45(16):3945-3951
In order to observe the anti-tumor effect of cinobufotalin on H22 liver cancer mice and to explore its regulatory mechanism, 50 Kunming mice were subcutaneously inoculated with H22 intraperitoneal passage cells under the armpit to establish H22 hepatocellular carcinoma model. They were then randomly divided into model group, cinobufotalin low dose group, cinobufotalin high dose group, cisplatin group and cisplatin+cinobufotalin group, which received 0.01% ethanol solution, 1 mg·kg~(-1) cinobufotalin, 5 mg·kg~(-1) cinobufotalin, 5 mg·kg~(-1) cisplatin, 5 mg·kg~(-1)cisplatin + 5 mg·kg~(-1) cinobufotalin respectively for 10 days. The general condition of mice during the intervention was observed, and the inhibition rate, tumor mass, thymus index, histopathological changes of the tumors, apoptotic rate of the tumors, the expressions of phosphatidylinositol 3-kinase(PI3 K), protein kinase B(Akt), apoptosis related gene(Fas), Fas ligand(FasL) mRNA and protein phosphorylated Akt(pAkt) protein in the tumors of each group were compared. The results showed that during the modeling period, the mice showed a decline in food intake, dark fur, poor mental status, and gradually worsened over time. The mental status of mice in each intervention group was improved gradually, especially in the cisplatin+cinobufotalin group. As compared with the model group, the tumor mass of each intervention group was lower(P<0.05). As compared with the cinobufotalin low dose group, the tumor mass was lower and inhibition rate was higher in the cinobufotalin high dose group, cisplatin group and cisplatin+cinobufotalin group(P<0.05). As compared with the cinobufotalin high dose group and the cisplatin group, the tumor mass was lower and the inhibition rate was higher in cisplatin+cinobufotalin group(P<0.05). As compared with the model group, the thymus index was higher in cinobufotalin high dose group and cisplatin + cinobufotalin group, while was lower in cisplatin group(P<0.05). As compared with the cinobufotalin low dose group, the thymus index was higher in the cinobufotalin high dose group and lower in the cisplatin group(P<0.05). As compared with the cinobufotalin high dose group, the thymus index was lower in cisplatin group(P<0.05). As compared with cisplatin group, the thymus index was higher in cisplatin+cinobufotalin group(P<0.05). Pathological staining showed that a large number of heterogeneous cells and mitotic phenomena were observed in the model group. Cell fragments and neutrophils were observed in the tumor tissues of the intervention groups, showing diffuse necrosis, and the diffuse necrosis was more obvious in the cisplatin+cinobufotalin group. As compared with the model group, the apoptotic rate of the tumors and the relative expressions of Fas mRNA and protein were higher in the intervention groups, while the relative expressions of PI3 K, FasL mRNA and protein and the relative expression of pAkt protein were lower in the intervention groups(P<0.05). As compared with the cinobufotalin low dose group, the apoptotic rate of the tumors and relative expression of Fas and protein were higher in the cinobufotalin high dose group, cisplatin group and cisplatin+cinobufotalin group, while the relative expressions of PI3 K, FasL mRNA and protein and pAkt protein were lower(P<0.05). As compared with the cinobufotalin high dose group and the cisplatin group, apoptotic rate of the tumors and the relative expression of Fas mRNA and protein were higher in the cisplatin+cinobufotalin group, while the relative expressions of PI3 K, FasL mRNA and protein and pAkt protein were lower in the cisplatin+cinobufotalin group(P<0.05). In summary, cinobufotalin has significant anti-tumor effect on H22 liver cancer mice, and can enhance the immune function of mice and synergistically enhance the effect of chemotherapy. Its mechanism may be associated with regulating PI3 K/Akt/Fas/FasL signaling pathway related genes and protein expression.
Animals
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Apoptosis
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Bufanolides
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Carcinoma, Hepatocellular
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Cisplatin
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Fas Ligand Protein
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Liver Neoplasms
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Mice