1.Diagnostic value of sterility sputum aspirating tube and bronchoalveolar lavage by bronchofibroscope in mechanically ventilated patients with lower respiratory tract infection.
Huan YANG ; Ping CHEN ; Hui JIANG ; Hong LUO
Journal of Central South University(Medical Sciences) 2009;34(8):807-810
OBJECTIVE:
To compare the diagnostic value of sterility sputum aspirating tube with bronchoalveolar lavage in mechanically ventilated patients with lower respiratory infection.
METHODS:
Sixty-four mechanically ventilated patients with lower respiratory infection were selected to collect respiratory passage secretion to analyze the etiology and to evaluate the diagnostic value by using sterility tube aspirate sputum and bronchoalveolar lavage.
RESULTS:
The positive rate of sterility tube aspirate sputum and bronchoalveolar lavage was 71.88% and 78.13%, respectively, with no statistical difference between the 2 groups (P>0.05). The consistency rate was 81.25% for the 2 methods.
CONCLUSION
Sterility sputum aspirating tube can not only acquire accurate pathogen, provide evidence to select sensitive antibiotics in clinical practice, but also is a simple practice, safe and economical method for patients with lower respiratory tract infection and mechanical ventilation.
Adult
;
Aged
;
Aged, 80 and over
;
Bronchoalveolar Lavage Fluid
;
microbiology
;
Bronchoscopes
;
Female
;
Humans
;
Male
;
Middle Aged
;
Pneumonia
;
diagnosis
;
etiology
;
microbiology
;
Respiration, Artificial
;
adverse effects
;
Sputum
;
microbiology
;
Suction
;
instrumentation
;
methods
;
Young Adult
2.Pseudo-outbreak of Stenotrophomonas maltophilia Due to Contamination of Bronchoscope.
Gyun Yeol AHN ; Feng Nan YU ; Sook Jin JANG ; Dong Min KIM ; Geon PARK ; Dae Soo MOON ; Young Jin PARK
The Korean Journal of Laboratory Medicine 2007;27(3):205-209
BACKGROUND: We noticed an abrupt increase in the isolation of Stenotrophomonas maltophilia from bronchoalveolar lavage (BAL) specimens collected at Chosun University Hospital. We performed surveillance cultures in order to identify the source of what appeared to be a pseudo-outbreak. METHODS: To investigate a possible nosocomial outbreak of S. maltophilia, we performed culture of 11 environmental specimens obtained from a bronchoscopy room and two bronchoscopes. Pulsedfield gel electrophoresis (PFGE) was used to examine the genetic relatedness among the strains of S. maltophilia recovered from BAL specimens of 3 patients and 1 environmental sample, as well as 9 unrelated strains of S. maltophilia as a control. RESULTS: During a 7 day-period in March 2006, S. maltophilia was isolated from the BAL specimens of 7 of 13 (54%) patients, compared to only 5 of 188 (2.6%) patients during the 6-month period prior to that period. S. maltophilia was isolated from 1 of the 11 environmental samples, which was obtained from a fiberoptic bronchoscope suction channel. All 7 patient isolates and one environmental isolate exhibited similar antibiotic susceptibility patterns. PFGE analysis of the genomic DNA from epidemic strains demonstrated an identical banding pattern, whereas each of epidemiologically unrelated strains showed a unique electrophoretic pattern. CONCLUSIONS: Apparently one of the hospital bronchoscopes became contaminated with S. maltophilia during a bronchoscopic procedure. It is likely that subsequent specimen contamination occurred because the bronchoscope had been inadequately cleaned and disinfected. The pseudo-outbreak was controlled successfully by removing the source of infection.
Aged
;
Aged, 80 and over
;
Bronchoalveolar Lavage Fluid/microbiology
;
Bronchoscopes/*microbiology
;
*Disease Outbreaks
;
Electrophoresis, Gel, Pulsed-Field
;
*Equipment Contamination
;
Gram-Negative Bacterial Infections/diagnosis/*epidemiology/transmission
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Humans
;
Male
;
Microbial Sensitivity Tests
;
Middle Aged
;
Stenotrophomonas maltophilia/*genetics/isolation & purification