Adult ; Bronchi ; pathology ; Foreign Bodies ; etiology ; Humans ; Male ; Neck Injuries ; etiology ; Trachea ; injuries

Tracheobronchial disruption is an uncommon injury associated with blunt chest trauma. We report CT features and pathologic findings of two pediatric cases in which a bronchial injury was unnoticed initially but was diagnosed later by appearance of delayed bronchostenosis with distal atelectasis after blunt chest trauma in recent motor vehicle accidents. Pathologically, obliteration of the bronchial lumen was caused by dense fibrous overgrowth and granulation tissue.
Wounds, Nonpenetrating/*diagnosis ; Tomography, X-Ray Computed/*methods ; Time Factors ; Thoracic Injuries/*diagnosis ; Male ; Humans ; Constriction, Pathologic ; Child, Preschool ; Child ; Bronchial Neoplasms/*pathology ; Bronchi/*injuries/pathology ; Accidents, Traffic

Adolescent ; Airway Obstruction ; Bronchi ; pathology ; Bronchography ; Child ; Diagnosis, Differential ; Foreign Bodies ; Humans ; Inhalation ; Male ; Obesity, Morbid ; complications ; Prader-Willi Syndrome ; complications ; Radiography, Thoracic ; Sleep Apnea, Obstructive ; complications ; diagnosis ; Thoracic Injuries ; diagnosis ; Tomography, X-Ray Computed ; Trachea ; pathology

To investigate the roles of glycogen synthase kinase 3beta (GSK3beta) and adenomatous polyposis coli (APC) protein in wound repair of airway epithelial cells (AECs), we established a wound model of airway epithelium in vitro. Then the following tests were undertaken: (1) Western blot was used to detect the levels of total GSK3beta and phosphorylated GSK3beta in human bronchial epithelial (16HBE) cells; (2) The localizations of APC protein was observed by using immunofluorescence technique; (3) Immunoprecipitation was used to investigate the relationship between APC protein and GSK3beta during the repair of 16HBE cells. The results were as follows: (1) The level of phosphorylated GSK3beta increased 0.5 h after scratching (P<0.05), reached a maximum at 6 h (P<0.05), and maintained until 12 h, while the total level of GSK3beta remained constant; (2) Results of immunofluorescence study showed that APC protein clustered with tubulin in the region of the migrating leading cells 6 h after scratching, which was dissimilar with that in the cells 0 h after scratching; (3) GSK3beta and APC protein were immunoprecipitated and analysed on SDS-PAGE. We found that GSK3beta and APC protein were precipitated, indicating that the two proteins existed in a complex. After scratching, dissociation of the two proteins occurred. Taken together, we conclude that scratching caused a decrease in phosphorylation of GSK3beta, and that reduced phosphorylation of GSK3beta promoted APC protein to bind to the plus ends of microtubules and stabilize the growing ends. These observations suggest that APC protein and GSK3beta may synergistically play an important role in the repair of airway epithelium.
Adenomatous Polyposis Coli Protein ; physiology ; Bronchi ; cytology ; injuries ; Cell Line ; Epithelial Cells ; metabolism ; pathology ; Glycogen Synthase Kinase 3 ; physiology ; Glycogen Synthase Kinase 3 beta ; Humans ; Phosphorylation ; Wound Healing ; physiology